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1MicroRNA let-7b targets important cell cycle molecules in malignant melanoma cells and interferes with anchorage-independent growth显示文摘一幅 microRNA 表示屏幕被执行从良性的 melanocytic nevi 在 laser-microdissected 纸巾分析 157 不同 microRNAs (n = 10 ) 并且主要恶意的黑瘤(n = 10 ) ,用量的即时 PCR。微分表示被作出对有利的裁决 72 microRNAs。microRNAs 的 let-7 家庭的成员是显著地, down 作为与良性的 nevi 相比在主要黑瘤调整了,为是的这些分子的一个可能的角色暗示肿瘤在恶意的黑瘤压制 ors。有趣地,类似的调查结果为肺和结肠癌被描述了。在黑瘤房间试管内 down 的 let-7b 的 Overexpression 调整了 cyclins 的表示 D1, D3,和 A,和 cyclin 依赖的 kinase (Cdk ) 4,所有哪个被描述了在黑瘤开发起一个作用。蛋白质表示上的 let-7b 的效果由于单个 mRNAs 的 3'-untranslated 区域(3'UTRs ) 指向,为 cyclin D1 由记者基因分析例示。与它细胞周期管理者上的 down modulating 效果一致, let-7b 禁止了细胞周期前进和黑瘤细胞的抛锚无关的生长。总起来说,这些调查结果不仅指向早黑瘤开发的新规章的机制,而且可以打开大街因为未来指向了这个肿瘤的治疗。Julia Schultz Peter Lorenz Gerd Gross Saleh Ibrahim Manfred Kunz 2008Cell Research2008,18,5:45
2Thulium:YAG VapoEnucleation of the prostate in large glands: a prospective comparison using 70- and 120-W 2-μm lasers显示文摘这研究比较了 70-w 和 120-w 2-µ 的功效; m 铥: 为有良性的 prostatic 阻塞(BPO ) 的病人的前列腺(ThuVEP ) 的钇铝柘榴石 VapoEnucleation。有征兆的 BPO 和 prostatic 增大的 84 个病人的未来的分析(≥ 60   ml ) 非随机经历了 70-w (n=44 ) 或 120-w ThuVEP (n=40 ) 的人,被执行。耐心的人口分布和 perioperative 和 12 月的后续数据被分析。吝啬的前列腺体积是 79.90 ± 27.49  在谁的病人的 ml 收到了 70-w ThuVEP,它是不到在里面收到了 120-w ThuVEP 的那些(88.53 ± 25.10;P=0.033 ) 。吝啬的 enucleation (resected 重量 / 激光时间, 2.16 ± 1.21   g min −1 对 1.23 ± 0.60   g min −1 ;P=0.013 ) ,操作效率(resected 重量 / 总数操作时间, 0.76 ± 0.35   g min −1 对 0.42 ± 0.27   g min −1 ;P=0.000 ) 并且 resected 织物的百分比(66.93 % ± 22.79 %对 45.41 % ± 23.33 %P=0.000 ) 与与 70-w ThuVEP 相比的 120-w 治疗是更高的。一个病人(1.2 %彻底的病人)( 在 120-w 组) 手术后地要求了输血。61 个病人(73 %) 为在 12 月的后续时间点的评论是可得到的。生活( QoL )的质量,国际前列腺症状分数( IPSS ),最大的尿流动率( Q 最大), postvoiding 剩余尿( PVR )和前列腺体积在治疗以后显著地改善了( P≤ ; 0.035 )并且不在与不同设备(70-w)和 120-w )对待的那些之间是显著地不同的。中部的前列腺体积减小是 81.70 %并且 82.19 %与 70-w 和 120-w ThuVEP,分别地。复杂并发症的发生是低的并且没在与不同设备对待的组之间不同。二个病人(2.4 %)( 一个每组) 在后续有膀胱颈挛缩。ThuVEP 是为征兆的 BPO 的治疗的一个安全、有效的过程。复杂并发症的发生与两台设备是低的。120-w 铥: 钇铝柘榴石设备关于 resected 织物和 enucleation/operation 效率的百分比提高 ThuVEP 的有效性。Christopher Netsch Thorsten Bach Thomas RW Herrmann Andreas J Gross 2012Asian Journal of Andrology2012,14,2:18
3微小RNA-1和微小RNA-29b对急性心肌梗死后不良心室重塑的诊断和预后价值显示文摘已知微小RNA(microRNAs,miRs)在急性心肌梗死(acute myocardial infarction,AMI)临床前期模型中可发挥纤维化和抗纤维化作用。为了评估miR-1,miR-21,miR-29b和miR-92a能否作为患者发生AMI后不良心室重塑(adverse ventricular remodeling,AVR)的循环生物标志物,刘莉 叶鹏 Grabmaier U Clauss S Gross L Klier I Franz WM Steinbeck G Wakili R Theiss HD Brenner C 2017中华高血压杂志2017,25,7:17
4基于热传导逆问题方法预测材料热物性参数显示文摘建立了一种应用LevenbergMarquardt迭代过程求解非线性热传导逆问题用于预测材料热物性的方法.以平板内的一维非稳态热传导为研究对象,在准确的数值计算结果上施加非均匀随机误差来模拟瞬态温度实验数据,应用该方法分别对热传导系数为常数、热传导系数和容积比热容为常数以及热传导系数和容积比热容为温度的函数3种情况进行了预测.参数预测结果表明,方法对基于瞬态实验方法进行热物性预测具有良好的适应性,并适用于分析多类热传导逆问题,该方法还能为优化实验提供有效信息.杨晨 Ulrich Gross 2005化工学报2005,56,12:13
5Metastatic type 1 gastric carcinoid:A real threat or just a myth?显示文摘AIM:To describe disease characteristics and treatment modalities in a group of rare patients with metastatic gastric carcinoid type 1(GCA1).METHODS:Information on clinical,biochemical,radiological,histopathological findings,the extent of the disease,as well as the use of different therapeutic modalities and the long-term outcome were recorded.Patients’data were assessed at presentation,and thereafter at 6 to 12 monthly intervals both clinically and biochemically,but also endoscopically and histopathologically.Patients were evaluated for the presence of specific symptoms;the presence of autoimmune disorders and the presence of other gastrointestinal malignancies in other family members were also recorded.The evaluation of response to treatment was defined using established WHO criteria.RESULTS:We studied twenty consecutive patients with a mean age of 55.1 years.The mean follow-up period was 83 mo.Twelve patients had regional lymph node metastases and 8 patients had liver metastases.The primary tumor mean diameter was 20.13±10.83mm(mean±SD).The mean Ki-67 index was 6.8%±11.2%.All but one patient underwent endoscopic or surgical excision of the tumor.The disease was stable in all but 3 patients who had progressive liver disease.All patients remained alive during the follow-up period.CONCLUSION:Metastatic GCA1 carries a good overall prognosis,being related to a tumor size of≥1 cm,an elevated Ki-67 index and high serum gastrin levels.Simona Grozinsky-Glasberg Dimitrios Thomas Jonathan R Strosberg Ulrich-Frank Pape Stephan Felder Apostolos V Tsolakis Krystallenia I Alexandraki Merav Fraenkel Leonard Saiegh Petachia Reissman Gregory Kaltsas David J Gross 2013World Journal of Gastroenterology2013,19,46:11
6逝去的时间:论晚期现代文化中的记忆与遗忘显示文摘一 记忆所承载的丰富内涵 自19世纪晚期以来,心理学家对记忆的研究富有成效,提出了记忆的几种类型:第一是语义记忆(semantic memories),它能使回忆词语及其意义成为可能,同样也能在这个词和那个词之间建立联系;第二是命题记忆(propositional memories),它能使人回忆起特定类型的信息。大卫·格罗斯(David Gross) 和磊(编译) 2011文化研究2011,,1:11
7Characterization of a critical role for CFTR chloride channels in cardioprotection against ischemia/ reperfusion injury显示文摘目的: 进一步描绘膀胱的纤维变性 transmembrane 传导力管理者(CFTR ) 的功能的角色在早、迟了(第二扇窗户) ischemic preconditioning (欧洲电脑生产厂商)- 并且 postconditioning (停靠港) 调停了对 ischemia/reperfusion (I/R ) 的 cardioprotection 损害。Sunny Yang XIANG Linda L YE Li-lu Marie DUAN Li-hui LIU Zhi-dong GE John A AUCHAMPACH Garrett J GROSS Dayue Darrel DUAN 2011Acta Pharmacologica Sinica2011,32,6:9
8Differing and isoform-specific roles for the formin DIAPH3 in plasma membrane blebbing and filopodia formation显示文摘血浆膜(下午) 水泡是发生的动态充满肌动朊的房间伸出,例如在胞质分裂期间,似变形虫的房间活动性和房间附件。用对 21 个肌动朊成核因素的一幅指向的 siRNA 屏幕,我们在房间粘附期间在下午 blebbing 识别一篇小说和人的透明 formin DIAPH3 的必要角色。DIAPH3 的抑制禁止了 blebbing 支持快速的房间传播包含 β 1-integrin。DIAPH3 的多重 isoforms 在 mRNA 和 isoforms, 3 和 7 是然而没导致充满肌动朊的伸出的形成的最大、很丰富的 isoforms 的蛋白质水平上被检测。更确切地说,下午 blebbing 明确地包含了低丰富 isoform 7 由透明自动调整的领域的删除引起了 filopodia 的形成的 isoform 的 1 DIAPH3 和激活。Dimerization 和肌动朊集会活动为由 DIAPH3 isoforms 的特定的房间伸出的正式就职是必要的 1 和 7。我们的数据建议包括 GTPase 有约束力的域的 N 终端区域象它在水泡和 filopodia 之间的伸出活动一样决定了 formin 的 subcellular 本地化。我们建议由 DIAPH3 的 isoform 选择的肌动朊汇编施加特定并且调整的差别在房间粘附和活动性期间工作。Jana Stastna Xiaoyu Pan Haicui Wang Alina Kollmannsperger Stefan Kutscheidt Volker Lohmann Robert Grosse Oliver T Fackler 2012Cell Research2012,22,4:6
9Extending the functions of the homeotic transcription factor Cdx2 in the digestive system through nontranscriptional activities显示文摘The homeoprotein encoded by the intestinal-specific Cdx2 gene is a major regulator of gut development and homeostasis,also involved in colon cancer as well as in intestinal-type metaplasias when it is abnormally expressed outside the gut.At the molecular level,structure/function studies have demonstrated that the Cdx2 protein is a transcription factor containing a conserved homeotic DNA-binding domain made ofthree alpha helixes arranged in a helix-turn-helix motif,preceded by a transcriptional domain and followed by a regulatory domain.The protein interacts with several thousand sites on the chromatin and widely regulates intestinal functions in stem/progenitor cells as well as in mature differentiated cells.Yet,this transcription factor also acts trough original nontranscriptional mechanisms.Indeed,the identification of novel protein partners of Cdx2 and also of a splicing variant revealed unexpected functions in the control of signaling pathways like the Wnt and NF-k B pathways,in double-strand break DNA repair and in premessenger RNA splicing.These novel functions of Cdx2 must be considered to fully understand the complexity of the role of Cdx2 in the healthy intestine and in diseases.Jean-Noel Freund Isabelle Duluc Jean-Marie Reimund Isabelle Gross Claire Domon-Dell 2015World Journal of Gastroenterology2015,21,5:5
10A systematic review and meta-analysis of clinical trials implementing aromatase inhibitors to treat male infertility显示文摘Aromatase activity has commonly been associated with male infertility characterized by testicular dysfunction with low serum testosterone and/or testosterone to estradiol ratio.In this subset of patients,and particularly in those with hypogonadism,elevated levels of circulating estradiol may establish a negative feedback on the hypothalamic–pituitary–testicular axis by suppressing follicle-stimulating hormone(FSH)and luteinizing hormone(LH)production and impaired spermatogenesis.Hormonal manipulation via different agents such as selective estrogen modulators or aromatase inhibitors to increase endogenous testosterone production and improve spermatogenesis in the setting of infertility is an off-label option for treatment.We carried out a systematic review and meta-analysis of the literature of the past 30 years in order to evaluate the benefits of the use of aromatase inhibitors in the medical management of infertile/hypoandrogenic males.Overall,eight original articles were included and critically evaluated.Either steroidal(Testolactone)or nonsteroidal(Anastrozole and Letrozole)aromatase inhibitors were found to statistically improve all the evaluated hormonal and seminal outcomes with a safe tolerability profile.While the evidence is promising,future prospective randomized placebo-controlled multicenter trials are necessary to better define the efficacy of these medications.Francesco Del Giudice Gian Maria Busetto Ettore De Berardinis Isabella Sperduti Matteo Ferro Martina Maggi Martin S Gross Alessandro Sciarra Michael L Eisenberg 2020Asian Journal of Andrology2020,22,4:5
11一种变刚度转臂节点设计及试验研究显示文摘设计了一种新型的适用于轨道车辆转向架的具有变刚度特性的轴箱转臂节点,它由橡胶主簧、橡胶辅簧和液压机构组成。介绍了该节点的设计原理,论述了主要构件的结构设计思路和参数计算方法。基于结构参数计算结果完成样件试制,动静态刚度试验结果验证了结构方案的合理性,样件实现了低频低刚度和高频高刚度的变刚度特性。所述结构方案和试验方法为液体橡胶复合减振技术在轨道车辆上的工程化应用奠定了理论基础。邹波 Josef Gross 王涛 卜继玲 丁行武 2018铁道机车车辆2018,38,6:5
12A review of human carcinogens—Part B: biological agents显示文摘Véronique Bouvard Robert Baan Kurt Straif Yann Grosse Béatrice Secretan Fatiha El Ghissassi Lamia Benbrahim-Tallaa Neela Guha Crystal Freeman Laurent Galichet Vincent Cogliano 2009Lancet Oncology2009,,4:4
13DDGS和酵母培养物对奶牛生产性能和乳成分的影响显示文摘选择16头泌乳天数为127±52d经产荷斯坦泌乳牛用于评价酵母培养物(达农威益康XP)和玉米酒糟及其可溶物(DDGS)在试验日粮中含有限草料纤维数量(草料中的中性洗涤纤维含量为19.3%,干物质基础)之情况下对产奶量和乳成分的影响及其它们之间的互作效应。本次试验采用4重复4×4拉丁方设计,试验时间为4个星期。处理组均按照2×2因子排列而设定,其中:1)日粮中不含酵母培养物和DDGS(对照组);2)日粮中含有20%DDGS,无酵母培养物(DDGS组);3)日粮中含有60g/d的酵母培养物益康XP(XP组);4)日粮中含有20%DDGS和60g/d的益康XP(XP+DDGS组)。本次试验结果表明:各处理组之间对奶牛体重和体况评分无显著的影响。日粮的处理并不影响干物质采食量(DMI),在使用草料纤维含量较低且添加20%DDGS的日粮情况下乳脂率会降低。添加益康XP的确能够提高产奶量及乳蛋白产量,但却不能够完全阻止因DDGS所造成的乳脂率下降的趋势。无论日粮中是否含有DDGS,益康XP都能够提高奶牛的生产性能。AR Hippen DJ Schingoethe KF Kalscheur P Linke K Gross DR Rennich IIkye Yoon 徐明 2010中国奶牛2010,,8:4
14Sensitivity and specificity of different staining methods to monitor apoptosis induced by oxidative stress in adherent cells显示文摘Objective To study the sensitivity and specificity of different staining methods to monitor apoptosis induced by oxidative stress in adherent cells.Methods Sensitivity and specificity of several common methods for apoptosis determination were evaluated (Apo2.7-expression, Annexin V-binding, TUNEL-reaction, poly-(ADP-ribose)-polymerase-(PARP) cleavage and single-stranded-DNA (ssDNA) staining). Apoptosis was induced by oxidative stress generated by hydrogen peroxide in 3 cultured cells types growing as adherent monolayer (MiaPaCa-2, Hep-G2 and human skin fibroblasts), necrosis was induced by depletion of cellular ATP using sodium azide. Cells positively stained by the respective apoptosis assay were quantified and alterations of cell morphology were monitored by fluorescence microscopy. The date was analyzed by one-way analysis of variance and significance test of correlation coefficient.Results One hour after apoptosis induction significant cell fractions were positively stained for ssDNA (33% with MiaPaCa-2 cells, 35% with Hep-G2 cells, 56% with human skin fibroblasts). PARP-cleavage was less sensitive compared to the ssDNA-staining. Apo2.7-expression, Annexin V-binding and TUNEL-reaction were not applicable to detect early apoptosis induced by oxidative stress (below 2 hours), but were efficiently monitoring late apoptosis. Specificity of ssDNA-staining was complete with each cell type even 4 hs after induction of necrosis by the highest sodium azide concentration. In contrast, the same experimental conditions resulted in 50%-90% positively stained necrotic cells by using Apo2.7-expression, TUNEL-reaction or AnnexinV-binding. Surprisingly, specificity of PARP-cleavage was highly depending on the respective cell type.Conclusions Our study prove that among the five methods investigated only ssDNA-staining allowed to completely differentiate apoptosis from necrosis, and is thus suitable to reliably detect early as well as late apoptosis. Therefore, the ssDNA-staining may be used as reference method to clearly identify apoptosis induced by oxidative stress in adherent cells. The TUNEL-reaction, annexin-V-binding and Apo-2.7-expression may be used to quantify the number of apoptotic and necrotic cells especially at later stages but without discrimination of apoptosis and primary or secondary necrosis.赵进顺 Alexandra Schmid-Kotsas Hans-Juergen Gross Adolf Gruenert Max G. Bachem 2003Chinese Medical Journal2003,,12:4
15股骨近端同种异体骨移植治疗全髋关节置换术后VancouverB3型股骨假体周围骨折手术技术显示文摘背景:全髋关节置换术后的股骨假体周围骨折的发生率日益增高,当股骨近端骨量不足并出现骨折时,能够选择的翻修手术方法则不多,其中一种方法就是股骨近端同种异体骨移植术。方法:我们回顾性评估采用股骨近端同种异体骨移植术治疗24例VancouverB,型假体周围骨折患者(25髋)的随访结果和并发症,平均随访时间为5.1年,临床效果采用Harris评分,放射学评估指标包括转子的融合程度、宿主骨和移植骨的融合程度、是否出现移植骨的吸收、是否出现假体的松动或断裂,以须行清除移植骨的翻修手术作为失败的标准。Catherine F. Kellett Petros J. Boscainos Anthony C. Maury Ari Pressman Barry Cayen Paul Zalzal David Backstein Allan Gross 史占军(译) 郭予立(译) 2008中华骨科杂志2008,28,3:4
16Online single particle analysis of chemical composition and mixing state of crop straw burning particles: from laboratory study to field measurement显示文摘新鲜稻草烧(SB ) 粒子被米饭的燃烧在实验室产生稻草和玉米稻草。化学作文和新鲜 SB 粒子的混合状态被一个喷雾器蒂米夫莱特·马斯分光计(ATOFMS ) 调查。基于团光谱模式, SB 粒子被聚类进四种主要类型:盐,器官的碳(OC ) ,元素的碳(EC ) ,和 EC 和 OC (EC-OC ) 的内部混合的粒子。另外,包含灰,多不的芳香的烃,重金属或烟碱的粒子也被观察。SB 粒子的物理、化学的变化立即与高度解决时间的数据在排放以后被分析。在变老的过程期间,平均粒子尺寸稳定地增加了。刚射出的有机化合物逐渐地在包含 OC 粒子被氧化到更多的氧化混合物。同时,重要排水量反应(2KCl + 那么 42 K 24+ 2Cl ) 因此被观察。为 SB 粒子的标记离子被优化并且适用在周围的空气识别 SB 粒子。ATOFMS 排序的周围的 SB 粒子的数字部分的变化与可溶的 K + 由联机离子层析测量了的水的同意很好,证明优化标记离子能是为在地大小的 SB 粒子的好 tracers。Juntao HUO Xiaohui LU Xinning WANG Hong CHEN Xingnan YE Song Gao Deborah S. Gross Jianmin CHEN Xin YANG 2016Frontiers of Environmental Science & Engineering2016,10,2:4
17急性儿茶酚胺暴露引起可逆的无心脏细胞再生的心肌细胞损伤显示文摘儿茶酚胺增加心脏收缩力,但暴露于高浓度儿茶酚胺或长时间暴露可引起心脏损伤。最近的一项研究表明,小鼠单次皮下注射异丙肾上腺素(isoproterenol,ISO;200 mg/kg)导致急性心肌细胞死亡(8%-10%),但在1个月之内心脏完整修复。心脏再生是通过内源性原癌基因蛋白质cKit+心脏干细胞介导新的心肌细胞形成。刘莉 叶鹏 Wallner M Duran JM Mohsin S Troupes CD Vanhoutte D Borghetti G Vagnozzi RJ Gross P Yu D Trappanese DM Kubo H Toib A Sharp TE Harper SC Volkert MA Starosta T Feldsott EA Berretta RM Wang T Barbe MF Molkentin JD Houser SR 2016中华高血压杂志2016,24,8:4
18Subsurface scattering using splat-based diffusion in point-based rendering显示文摘Point-based graphics has gained much attention as an alternative to polygon-based approaches because of its simplicity and flexibility. However, current point-based techniques do not provide a sufficient rendering quality for translucent materials such as human skin. In this paper, we propose a point-based framework with subsurface scattering of light, which is important to create the soft and semi-translucent appearance of human skin. To accurately simulate subsurface scattering in multilayered materials, we present splat-based diffusion to apply a linear combination of several Gaussian basis functions to each splat in object space. Compared to existing point-based approaches, our method offers a significantly improved visual quality in rendering human faces and provides a similar visual quality to polygon-based rendering using the texture space diffusion technique. We demonstrate the effectiveness of our approach in rendering scanned faces realistically.KIM Hyeon-Joong BICKEL Bernd GROSS Markus CHOI Soo-Mi 2010Science China(Information Sciences)2010,53,5:3
19Endocrine radionuclide scintigraphy with fusion single photon emission computed tomography/computed tomography显示文摘AIM: To review the benefits of single photon emission computed tomography(SPECT)/computed tomography(CT) hybrid imaging for diagnosis of various endocrine disorders.METHODS: We performed MEDLINE and Pub Med searches using the terms: 'SPECT/CT'; 'functional anatomic mapping'; 'transmission emission tomography'; 'parathyroid adenoma'; 'thyroid cancer'; 'neuroendocrine tumor'; 'adrenal'; 'pheochromocytoma'; 'paraganglioma'; in order to identify relevant articles published in English during the years 2003 to 2015. Reference lists from the articles were reviewed to identify additional pertinent articles. Retrieved manuscripts(case reports, reviews, meta-analyses and abstracts) concerning the application of SPECT/CT to endocrine imaging were analyzed to provide a descriptive synthesis of the utility of this technology.RESULTS: The emergence of hybrid SPECT/CT camera technology now allows simultaneous acquisition of combined multi-modality imaging, with seamless fusion of three-dimensional volume datasets. The usefulness of combining functional information to depict the biodistribution of radiotracers that map cellular processes of the endocrine system and tumors of endocrine origin, with anatomy derived from CT, has improved the diagnostic capability of scintigraphy for a range of disorders of endocrine gland function. The literature describes benefits of SPECT/CT for ^(99m)Tc-sestamibi parathyroid scintigraphy and ^(99m)Tc-pertechnetate thyroid scintigraphy, ^(123)I- or ^(131)I-radioiodine for staging of differentiated thyroid carcinoma, ^(111)In- and ^(99m)Tclabeled somatostatin receptor analogues for detection of neuroendocrine tumors, ^(131)I-norcholesterol(NP-59) scans for assessment of adrenal cortical hyperfunction, and ^(123)I- or ^(131)I-metaiodobenzylguanidine imaging for evaluation of pheochromocytoma and paraganglioma.CONCLUSION: SPECT/CT exploits the synergism between the functional information from radiopharmaceutical imaging and anatomy from CT, translating to improved diagnostic accuracy and meaningful impact on patient care.Ka-Kit Wong Arpit Gandhi Benjamin L Viglianti Lorraine M Fig Domenico Rubello Milton D Gross 2016World Journal of Radiology2016,8,6:3
20MicroRNAs in the Human Heart: A Clue to Fetal Gene Reprogramming in Heart Failure显示文摘Thomas Thum Paolo Galuppo Christian Wolf Jan Fiedler Susanne Kneitz Linda W. van Laake Pieter A. Doevendans Christine L. Mummery Jürgen Borlak Axel Haverich Carina Gross Stefan Engelhardt Georg Ertl Johann Bauersachs 2007Circulation2007,,3:3
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