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| 1 | Virulence factors of Enterococcus strains isolated from patients with inflammatory bowel disease显示文摘AIM: To determine the features of Enterococcus that contribute to the development and maintenance of the inflammatory process in patients with inflammatory bowel disease (IBD). METHODS: Multiplex polymerase chain reaction (PCR) was applied to assess the presence of genes that encode virulence factors [surface aggregating protein (asa1), gelatinase (gelE), cytolysin (cylA), extracellular surface protein (esp) and hyaluronidase (hyl)] in the genomic DNA of 28 strains of Enterococcus isolated from the intestinal tissues of children with IBD (n =16) and of children without IBD (controls; n = 12). Additionally, strains with confirmed presence of the gelE gene were tested by PCR for the presence of quorum sensing genes (fsrA, fsrB, fsrC) that control the gelatinase production. Gelatinase activity was tested on agar plates containing 1.6% gelatin. We also analysed the ability of Enterococcus strains to release and decompose hydrogen peroxide (using Analytical Merckoquant peroxide test strips) and tested their ability to adhere to Caco-2 human gut epithelium cells and form biofilms in vitro. RESULTS: A comparison of the genomes of Enterococcus strains isolated from the inflamed mucosa of patients with IBD with those of the control group showed statistically significant differences in the frequency of theasa1 gene and thegelE gene. Furthermore, the cumulative occurrence of different virulence genes in the genome of a single strain ofEnterococcus isolated from the IBD patient group is greater than in a strain from the control group, although no significant difference was found. Statistically significant differences in the decomposition of hydrogen peroxide and adherence to the Caco-2 epithelial cell line between the strains from the patient group and control group were demonstrated. The results also showed that profuse biofilm production was more frequent amongEnterococcus strains isolated from children with IBD than in control strains. CONCLUSION: Enterococcus strains that adhere strongly to the intestinal epithelium, form biofilms and possess antioxidant defence mechanisms seem to have the greatest influence on the inflammatory process. | Edyta Golińska Anna Tomusiak Tomasz Gosiewski Gra yna Wiecek Agnieszka Machul Diana Mikoajczyk Magorzata Bulanda Piotr B Heczko Magdalena Strus | 2013 | World Journal of Gastroenterology2013，19，23： | 8 |
| 2 | Structural basis for enabling T-cell receptor diversity within biased virus-specific CD8 T-cell re- sponses显示文摘 | Day E B Guillonneau C Gras S | 2011 | Proc Natl Acad Sci USA2011，108，23： | 1 |
| 3 | Clinical trial:a nutritional supplement Viusid,in combination with diet and exercise,in patients with nonalcoholic fatty liver disease显示文摘 | Vilar gomez E Rodriguez de miranda A Gra oramas B | | 0，，10： | 1 |
| 4 | Premature rupture of membranes before 32 weeks of gestation: Prenatal prognosis factors 显示文摘 | PaumierA Gras - Leguen C Branger B | 2008 | Gynecol Obstet Ferti12008，36，7： | 1 |
| 5 | Choristoma of visual organ in children显示文摘 | Niwald A Orawiec B Graek M | | 0，，7： | 1 |
| 6 | International Global At- mospheric Chemistry (IGAC) project' s First Aerosol Characteriza- tion Experiment ( ACE 1 ) : Overview 显示文摘 | Bates T S Huebert B J Gras J L | 1998 | J Geophys Res1998，103，16： | 1 |
| 7 | Contribution of the Chemical Structure of wheat starch to Japanese noodle quality显示文摘 | Batey I L Gras P W Curtin B M | 1997 | Journal of the Science of Food and Agriculture1997，74，： | 1 |
| 8 | The vesicular glutamate transporter VGLUT3 synergizes striatal acetylcholine tone 显示文摘 | Gras C Amilhon B Lepieard E M | 2008 | Nat Neurosci2008，11，3： | 1 |
| 9 | A low-cost high efficiency solar cell based on dye-sensitized colloidal TiO2 films 显示文摘 | O' REGAN B GRA TZEL M | 1991 | Nattwe1991，353，6346： | 1 |
| 10 | Inherent Increase of Apoptosis in Liver Tumors:Implication for Carcinogenosis and Tumor Regression显示文摘 | Gras Kraupp B Ruttay-Nedecky B Mlauer L | 1997 | Hepatology1997，25，4： | 1 |
| 11 | A low-cost,high-efficiency solar cell based on dye-sensitized colloidal TiO2 films显示文摘 | B Gra¨tzel M | 1991 | Nature1991，353，： | 1 |
| 12 | Milling properties as method of reversible reduction and oidization of doughs显示文摘 | Bekes F Gras P W Gupta R B | 1994 | Cereal Chemistry1994，71，： | 1 |
| 13 | Semaphorin 7A protein variants differentially regulate T-cell activity显示文摘 | Gras C Eiz-Vesper B Seltsam A | | 0，，02： | 1 |
| 14 | A new PMMA-microchip device for isotachophoresis with integrated conductivity detector显示文摘 | Graβ B Neyer A Joehnck M | 2001 | Sensors and Actuators B2001，72，3： | 1 |
| 15 | Electrochemical deposition of smooth and homogeneously mesoporous ZnO films from propylene carbonate electrolytes 显示文摘 | O'Regan B Sklover V Gra tzel M | 2001 | J Electrochem Soc2001，148，7： | 1 |
| 16 | Next generation sequencing for molecular diagnosis of neuromuscular diseases显示文摘 | Vasli N B(o)hm J Le Gras S | 2012 | Acta Neuropathol2012，124，2： | 1 |
| 17 | The mixed phase of gearbox synchromesh operation显示文摘 | Paffoni B Progri R Gras R | 2000 | Proceedings of the Institution of Mechanical Engineers Part J:Journal of Engineering Tribology2000，214，2： | 1 |
| 18 | Treat -ment of refractory congestive heart failure by cardiac resynchronization显示文摘 | Ruffy R Cebron JP Brunel P Leurent B Banus Y | 2003 | Minerva Cardioangiol2003，51，2： | 1 |
| 19 | Transformation of pasta wheat (Triticum turgidum L. var. durum) with high-molecular-weight glutenin subunit genes and modification of dough functionality显示文摘 | G.Y. He L. Rooke S. Steele F. Békés P. Gras A.S. Tatham R. Fido P. Barcelo P.R. Shewry P.A. Lazzeri | 1999 | Molecular Breeding1999，，4： | 1 |
| 20 | Contribution of the chemical structure of wheat starch to Japanese noodle quality 显示文摘 | BATEY I L GRAS P W CURTIN B M | 1997 | Sci Food Agric1997，74，4： | 1 |
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