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| 1 | Lipogenesis in myoblasts and its regulation of CTRP6 by AdipoR1/Erk/PPARγ signaling pathway显示文摘在 C2C12 myoblasts 的导致的 lipogenesis 和它的规定仍然保持大部分不清楚。这里,我们发现鸡尾酒 methodcould 显著地通过调整类脂化合物导致 lipogenesis 在 myoblasts 的新陈代谢的基因和 Erk1/2 phosphorylation。同时, CTRP6 的表示和分泌物在宫外的 lipogenesis 期间被增加。而且, CTRP6 击倒下面调整 lipogenic 基因的层次和在早 lipogenic 的 phosphorylated Erk1/2 (p-Erk1/2 ) 上演,而在终端区别的起来调整的 p-Erk1/2。有趣地, CTRP6 siRNA 的效果在 myoblasts 是稀释 byU0126 (一个特殊 p-Erk1/2 禁止者) 。而且, AdipoR1,不是 AdipoR2,首先在有丝分裂的同种细胞的扩大的过程期间作为受体 ofCTRP6 被识别。一起,我们建议 CTRP6 调停通过在 myoblasts 表明小径的 AdipoR1/Erk/PPAR 的宫外的 lipogenesis。我们的调查结果将流轻在小说生物在 myoblast lipogenesis 期间 CTRP6 工作并且提供由 lipogenic regulationin 改进家畜的肉质量的一个有希望的方向骨胳的肌肉 myoblasts。 | Wenjing Wu Yunmei Sun Chen Zhao Cunzhen Zhao Xiaochang Chen Guoqiang Wang Weijun Pang Gongshe Yang | 2016 | Acta Biochimica et Biophysica Sinica2016，48，6： | 9 |
| 2 | Different Transcription Profiles of SOCS-3,ob and IGF-I Genes and their Possible Correlations in Obese and Lean Pigs显示文摘猪品种在胖免职和肌肉发展能力有重要差别。然而,在这些差别后面的分子的机制仍然是 unknown.Inthis 学习,三候选人基因的表示模式,压制或表明 3 的 cytokine ( SOCS-3 ),肥胖( ob )和像胰岛素的生长因素我( IGF-I ),它涉及脂肪质的新陈代谢或肌肉开发,被分析。全部的 RNA 从 8-month-old Bamei 的背面的下的脂肪质的组织和 longissimus 被提取, Largewhite pigs.Semiquantitative reversetranscription 聚合酶链反应被用来在肌肉组织在脂肪质的组织,和 SOCS-3 和 IGF-I 基因决定 SOCS-3and ob 基因的表示层次。结果证明在脂肪质的组织, SOCS-3 的表示水平在 Bamei 是显著地更高的(肥胖) 猪比那在 Largewhite (瘦) 猪(P<0.01 ) 。然而,在肌肉组织,它在 Largewhite 猪(P<0.01 ) 比那是显著地更低的 inBamei。而且, SOCS-3 的表示断然在肌肉组织在脂肪质的组织和 IGF-I 的被相关到 ob 的。这些调查结果建议在 SOCS-3 基因表达的差别铺平在脂肪质并且肌肉纸巾,在在肌肉组织的 SOCS-3 和 IGF-I 之间的在在脂肪质的组织的 SOCS-3 和 ob 之间的关系,和那,可能贡献在肥胖、瘦的猪之间的不同胖免职和肌肉发展能力。 | Jiangwei WU Bo WANG Haowei ZHANG Taiyong YU Gongshe YANG | 2007 | Acta Biochimica et Biophysica Sinica2007，39，4： | 6 |
| 3 | MiR-15a/b promote adipogenesis in porcine pre-adipocyte via repressing FoxO1显示文摘糖尿病和许多另外的新陈代谢症候群是仔细与肥胖有关。揭示胖免职的内在的机制,越来越多的研究在 adipocytes 开发期间正在集中于 miRNAs 的功能。以前的研究 haveproved 那出 miR-15a/b 戏在多重生理的过程的重要角色;然而,他们的功能在 adipogenesisremain 期间不清楚。揭示这,我们在猪的 pre-adipocyte 在 adipogenesis 期间检测了 miR-15a/b 的表示侧面,并且发现那他们层次在 adipocyte 区别的早阶段增加了并且在白天 4 .Moreover 以后落下的表示,在猪的 pre-adipocytes 的 miR-15a/b 的在表示上支持了 adipocyte 区别, miR-15a/b 的类脂化合物 accumulation.Target 基因被预言并且检验,它表明 Forkhead 盒子蛋白质 O1 ( FoxO1 )是目标 geneof miR-15a/b 。表示水平由 miR-15a/b 在表示上引起了的 FoxO1 的抑制在 adipogenesis.Thus 上有积极效果,我们断定 miR-15a/b 经由镇压在猪的 pre-adipocyte 支持 adipogenesis FoxO1。 | Peiyue Dong Yin Mai Zhenyu Zhang Lin Mi Guofang Wu Guiyan Chu Gongshe Yang Shiduo Sun | 2014 | Acta Biochimica et Biophysica Sinica2014，46，7： | 6 |
| 4 | MiR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine granulosa cells显示文摘Background: Granulosa cells(GCs) proliferation and estradiol synthesis significantly affect follicular development.The miR-214-3p expression in the ovarian tissues of high-yielding sows is higher than that in low-yielding sows,indicating that miR-214-3p may be involved in sow fertility. However, the functions and mechanisms of miR-214-3p on GCs are unclear. This study focuses on miR-214-3p in terms of the effects on GCs proliferation and estradiol synthesis.Results: Our findings revealed that miR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine GCs. MiR-214-3p can increase the percentage of S-phase cells, the number of EdU labeled positive cells, and cell viability. However, E2 concentration was reduced after miR-214-3p agomir treatment. We also found that miR-214-3p up-regulates the expression of cell cycle genes including cell cycle protein B(Cyclin B), cell cycle protein D(Cyclin D), cell cycle protein E(Cyclin E), and cyclin-dependent kinase 4(CDK4) at the transcription and translation levels, but down-regulates the mRNA and protein levels of cytochrome P450 family 11 subfamily A member 1(CYP11A1), cytochrome P450 family 19 subfamily A member 1(CYP19A1), and steroidogenic acute regulatory protein(StAR)(i.e., the key enzymes in estradiol synthesis). On-line prediction, bioinformatics analysis, a luciferase reporter assay, RT-qPCR, and Western blot results showed that the target genes of miR-214-3p in proliferation and estradiol synthesis are Mfn2 and NR5A1, respectively.Conclusions: Our findings suggest that miR-214-3 p plays an important role in the functional regulation of porcine GCs and therefore may be a target gene for regulating follicular development. | Shengjie Shi Xiaoge Zhou Jingjing Li Lutong Zhang Yamei Hu Yankun Li Gongshe Yang Guiyan Chu | 2021 | Journal of Animal Science and Biotechnology2021，12，1： | 3 |
| 5 | Origin of Gymnocypris przewalskii and phylogenetic history of Gymnocypris eckloni (Teleostei: Cyprinidae)显示文摘起源和种系发生的模式为 G 被估计。przexvalskii 和 G。由分析完全的 mtDNA 细胞色素 b 基因顺序(1140bp ) 的 eckloni。种系发生的分析进一步支持了那有三个 mtDNA 系(交流) ,在 G 识别。przewalskii 和 G。eckloni,证明第一鳃的外部 rakers 在 Gymnocypris 的发展史有很少意义钓鱼。建立的网络显示出那 G。黄河特定的 haplotype 艾尔的 eckloni 是一个创始人,它散发了 G 的所有 haplotypes。建议了 G 的 przexvalskii。przexvalskii 可能仅仅从 G 的二 maternals 之一发源。从黄河的 eckloni。F 测试和失配分析在 G 的人口显示出至少二个扩大事件。przexvalskii 大约 0.2734 妈并且 0。0658 妈,当时 G。ecklonifrom Qaidam 盆能经历了严重瓶颈效果大约 0.0693 妈。人口扩大与最近的普通祖先(TMRCA ) 在 subclades 艾尔和 A21 被检测大约 0.2308 ± 0 .01 妈和 0.1319 ± 0 .015 妈,分别地它在从上面的黄河引起了金海湖的分离的 Gonghe 运动事件的地质的年龄范围以内。这些结果建议了鱼多样化的效果由快速在迟了的更新世 Qing-hai-Tibetan 高原高举。 | Zhao Kai Duan Ziyuan Yang Gongshe Peng Zuogang He Shunping Chen Yiyu | 2007 | Progress in Natural Science:Materials International2007，17，5： | 3 |
| 6 | Sirt2 induces C2C12 myoblasts proliferation by activation of the ERK1/2 pathway显示文摘 | Guofang Wu Chengchuang Song Hongzhao Lu Long Jia Gongshe Yang Xin'e Shi Shiduo Sun | 2014 | Acta Biochimica et Biophysica Sinica2014，46，4： | 2 |
| 7 | Elevated miR-10a-5p facilitates cell cycle and restrains adipogenic differentiation via targeting Map2k6 and Fasn,respectively显示文摘miRNAs are a small class of noncoding RNAs that perform biological functions by regulating the stability or translation of target genes in various biological processes.This study illustrated the role of miR-10a-5p,which is relatively enriched in adipose tissues,using primary mouse preadipocytes as model.With elevated miR-10a-5p expression,the proliferative ability of mouse preadipocytes was significantly enhanced,indicated by increased EdU+cells and G1/S transition,accompanied by upregulated Cyclin B,Cyclin D and PCNA and downregulated p21 and p27.Meanwhile,the adi-pogenic differentiation was significantly attenuated by elevated miR-10a-5p,supported by Oil Red 0 staining and suppressed PPARγand aP2 expression.Furthermore,Map2k6 and Fasn were pre-dicted to be the target genes of miR-10a-5p in silico,and dual luciferase reporter assay confirmed the direct targeting effects.Western blot analysis results showed that miR-10a-5p specially reduced Map2k6 expression at the proliferative stage without affecting Fasn expression,while significantly restrained Fasn expression with unchanged Map2k6 expression during adipogenic differentiation.Taken together,these results revealed a potential role of miR-10a-5p in adipogenesis and in the treatment of obesity. | Xiaoyu Wang Huifang Zhang Meixue Xu Xin'E Shi Gongshe Yang Shiduo Sun Xiao Li | 2020 | Acta Biochimica et Biophysica Sinica2020，52，11： | 2 |
| 8 | A porphyr in pathway impairment is responsible for the phenotype of a dominant disease lesion mimic mutant of maize显示文摘 | Hu Gongshe Yalpani N Briggs S P | 1998 | Plant Cell1998，10，7： | 1 |
| 9 | Exosome-mediated regulatory mechanisms in skeletal muscle:a narrative review显示文摘Skeletal muscle plays a paramount role in physical activity,metabolism,and energy balance,while its homeostasis is being challenged by multiple unfavorable factors such as injury,aging,or obesity.Exosomes,a subset of extracellular vesicles,are now recognized as essential mediators of intercellular communication,holding great clinical potential in the treatment of skeletal muscle diseases.Herein,we outline the recent research progress in exosomal isolation,characterization,and mechanism of action,and emphatically discuss current advances in exosomes derived from multiple organs and tissues,and engineered exosomes regarding the regulation of physiological and pathological development of skeletal muscle.These remarkable advances expand our understanding of myogenesis and muscle diseases.Meanwhile,the engineered exosome,as an endogenous nanocarrier combined with advanced design methodologies of biomolecules,will help to open up innovative therapeutic perspectives for the treatment of muscle diseases. | Zhaolu WANG Jinjin YANG Xiaohui SUN Xi SUN Gongshe YANG Xin’e SHI | 2023 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2023，24，1： | 1 |
| 10 | Changes in DNA base sequences in the mutant of Arabidopsis thaliana induced by low-energy N+ implantation显示文摘 | Fengqi Chang Xuanming Liu Yinxin Li Gengxiang Jia Jingjing Ma Gongshe Liu Zhiqing Zhu | 2003 | Science in China Series C: Life Sciences2003，，5： | 1 |
| 11 | Over-expression of miR-125a-5p inhibits proliferation in C2C12 myoblasts by targeting E2F3显示文摘MicroRNAs miRNAs 是在长度的 2025 核苷酸的小非编码的 RNA 的一个班。miRNAs 在房间的许多类型的增长起重要作用,这被显示出,包括 myoblasts。在这研究,我们使用了即时量的聚合酶链反应,西方的弄污,在 C2C12 myoblasts 的增长期间探索 miR-125a-5p 的角色的 EdU,流动 cytometry,和 CCK-8 试金。miR-125a-5p 的表示在 C2C12 myoblast 增长期间被减少,这被发现。miR-125a-5p 的在表示上禁止了由 EdU 染色,流动 cytometry,和 CCK8 试金显示了的 C2C12 myoblast 增长。miR-125a-5p 能否定地在 posttranscriptional 水平调整 E2F3 表示,这也被发现,经由在 3 untranslated 区域的一个特定的目标地点。显示出的 E2F3 击倒 C2C12 myoblast 增长上的类似的禁止的效果。因此,我们的调查结果建议 miR-125a-5p 可以由指向 E2F3 充当 C2C12 myoblast 增长的一个否定管理者。 | Chengchuang Song Guofang Wu Aoqi Xiang Qiangling Zhang Wanhua Li Gongshe Yang Xin'e Shi Shiduo Sun Xiao Li | 2015 | Acta Biochimica et Biophysica Sinica2015，47，4： | 1 |
| 12 | Rho-associated protein k/nases play an important role in the differentiation of rat adipose-derived stromal ceils into cardiomyoeytes in vitro 显示文摘 | ZHAO Lili YANG Gongshe ZHAO Xin | 2014 | PLoSOne2014，9，11： | 1 |
| 13 | Enhanced tolerance to drought stress in transgenic tobacco plants overexpressing VTE1 for increased tocopherol production from Arabidopsis thaliana显示文摘 | Xiaoli Liu Xuejun Hua Juan Guo Dongmei Qi Lijuan Wang Zhipeng Liu Zhiping Jin Shuangyan Chen Gongshe Liu | 2008 | Biotechnology Letters2008，，7： | 1 |
| 14 | Chemical induction of apomictic seed formation in maize显示文摘 | Gongshe Hu G. H. Liang C. E. Wassom | 1991 | Euphytica1991，，2： | 1 |
| 15 | Enhanced drought tolerance in transgenic Leymus?chinensis plants with constitutively expressed wheat TaLEA 3显示文摘 | Lijuan Wang Xiaofeng Li Shuangyan Chen Gongshe Liu | 2009 | Biotechnology Letters2009，，2： | 1 |
| 16 | Progress of vitamin E metabolic engineering in plants显示文摘 | Shuangyan Chen Hongjie Li Gongshe Liu | 2006 | Transgenic Research2006，，6： | 1 |
| 17 | Progress of vitamin E metabolic engineering in plants 显示文摘 | Shuangyan Chen Hongjie Li Gongshe Liu | 2006 | Transgenic Research2006，15，： | 1 |
| 18 | Enhanced drought tolerance in transgenic Leymus?chinensis plants with constitutively expressed wheat TaLEA 3显示文摘 | Lijuan Wang Xiaofeng Li Shuangyan Chen Gongshe Liu | 2009 | Biotechnology Letters2009，，2： | 1 |
| 19 | microRNA‐151–3p Regulates Slow Muscle Gene Expression by Targeting ATP2a2 in Skeletal Muscle Cells显示文摘 | Huan Wei Zhongwen Li Xiaobin Wang Jie Wang Weijun Pang Gongshe Yang Qingwu W. Shen | 2015 | J. Cell. Physiol2015，，： | 1 |
| 20 | Phylogenetic relationships in Leymus (Poaceae: Triticeae) revealed by the nuclear ribosomal internal transcribed spacer and chloroplast trnL-F sequences显示文摘 | Zhipeng Liu Zhongyue Chen Jin Pan Xiaofeng Li Man Su Lijuan Wang Hongjie Li Gongshe Liu | 2007 | Molecular Phylogenetics and Evolution2007，，1： | 1 |
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