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| 1 | Diagnosis and treatment recommendations for pediatric respiratory infection caused by the 2019 novel coronavirus显示文摘Since December 2019,an epidemic caused by novel coronavirus (2019-nCoV) infection has occurred unexpectedly in China.As of 8 pm,31 January 2020,more than 20 pediatric cases have been reported in China.Of these cases,ten patients were identified in Zhejiang Province,with an age of onset ranging from 112 days to 17 years.Following the latest National recommendations for diagnosis and treatment of pneumonia caused by 2019-nCo V (the 4th edition) and current status of clinical practice in Zhejiang Province,recommendations for the diagnosis and treatment of respiratory infection caused by 2019-nCoV for children were drafted by the National Clinical Research Center for Child Health,the National Children's Regional Medical Center,Children's Hospital,Zhejiang University School of Medicine to further standardize the protocol for diagnosis and treatment of respiratory infection in children caused by 2019-nCoV. | Zhi-Min Chen Jun-Fen Fu Qiang Shu Ying-Hu Chen Chun-Zhen Hua Fu-Bang Li Ru Lin Lan-Fang Tang Tian-Lin Wang Wei Wang Ying-Shuo Wang Wei-Ze Xu Zi-Hao Yang Sheng Ye Tian-Ming Yuan Chen-Mei Zhang Yuan-Yuan Zhang | 2020 | World Journal of Pediatrics2020，16，3： | 45 |
| 2 | OsGRF4 controls grain shape, panicle length and seed shattering in rice显示文摘Traits such as grain shape, panicle length and seed shattering, play important roles in grain yield and harvest. In this study, the cloning and functional analysis of PANICLE TRAITS 2(PT2), a novel gene from the Indica rice Chuandali(CDL), is reported. PT2 is synonymous with Growth-Regulating Factor 4(OsGRF4), which encodes a growth-regulating factor that positively regulates grain shape and panicle length and negatively regulates seed shattering. Higher expression of OsGRF4 is correlated with larger grain, longer panicle and lower seed shattering. A unique OsGRF4 mutation, which occurs at the Osmi RNA396 target site of OsGRF4, seems to be associated with high levels of OsGRF4 expression, and results in phenotypic difference. Further research showed that OsGRF4 regulated two cytokinin dehydrogenase precursor genes(CKX5 and CKX1) resulting in increased cytokinin levels,which might affect the panicle traits. High storage capacity and moderate seed shattering of OsGRF4 may be useful in high-yield breeding and mechanized harvesting of rice. Our Resefindings provide additional insight into the molecular basis of panicle growth. | Pingyong Sun Wuhan Zhang Yihua Wang Qiang He Fu Shu Hai Liu Jie Wang Jianmin Wang Longping Yuan Huafeng Deng | 2016 | Journal of Integrative Plant Biology2016，58，10： | 16 |
| 3 | Associations of ABCB1, NFKB1, CYP3A, and NR112 polymorphisms with cyclosporine trough concentrations in Chinese renal transplant recipients显示文摘 | Yu ZHANG Jia-li LI Qian FU Xue-ding WANG Long-shan LIU Chang-xi WANG Wen XIE Zhuo-jia CHEN Wen-ying SHU Min HUANG | 2013 | Acta Pharmacologica Sinica2013，34，4： | 15 |
| 4 | The distribution characteristics of heavy metals in Guiyang urban soils显示文摘Guiyang is located in southwestern China,which is a famous tourism city,and is awarded the title of'the Summer Capital of China'.A study on heavy metals in Guiyang urban soils was conducted.The results showed that the concentrations of Cd,Cu,Ni,Pb and Zn in Guiyang urban soils are significantly higher than their background values and Cu,Zn and Pb in Guiyang soils are accumulated seriously.The distribution characteristics of heavy metals in 3 districts are described as follows:the level of heavy metals in soils follows the order of Nanming District> Yunyan District>Xiaohe District.The spatial distribution of metals in urban soils of Guiyang shows that the level of metals in the central and eastern parts of Guiyang is relatively high,and its characteristics are attributed to the influence of commercial activities,heavy traffic and industrial emission.Compared with some other cities in China,Guiyang is characterized by relatively high accumulation level of Cd,Cu,and Zn in its soils. | LI Xiaoyan CAO Yijin QI Le SHU Fu | 2012 | Chinese Journal Of Geochemistry2012，31，2： | 14 |
| 5 | Preliminary study on the production of transgenic mice harboring hepatitis B virus X gene显示文摘INTRODUCTIONDespiteoverwhelmingepidemiologicalevidencelinkingpersistentHepatitisBVirus(HBV)infectionandthedevelopmentofhepato... | ZHU Huan Zhang 1, CHENG Guo Xiang 2, CHEN Jian Qu 2, KUANG Shu Yuan 3, CHENG Yong 2, ZHANG Xin Li 1, Ll Hou Da 2, XU Shao Fu 2, SHI Jing Quan 1, QIAN Geng Sun 3 and GU Jian Ren 3 | 1998 | World Journal of Gastroenterology1998，4，6： | 14 |
| 6 | Insight-HXMT observations of the first binary neutron star merger GW170817显示文摘Finding the electromagnetic(EM) counterpart of binary compact star merger, especially the binary neutron star(BNS) merger,is critically important for gravitational wave(GW) astronomy, cosmology and fundamental physics. On Aug. 17, 2017,Advanced LIGO and Fermi/GBM independently triggered the first BNS merger, GW170817, and its high energy EM counterpart,GRB 170817 A, respectively, resulting in a global observation campaign covering gamma-ray, X-ray, UV, optical, IR, radio as well as neutrinos. The High Energy X-ray telescope(HE) onboard Insight-HXMT(Hard X-ray Modulation Telescope) is the unique high-energy gamma-ray telescope that monitored the entire GW localization area and especially the optical counterpart(SSS17 a/AT2017 gfo) with very large collection area(~1000 cm^2) and microsecond time resolution in 0.2-5 MeV. In addition,Insight-HXMT quickly implemented a Target of Opportunity(ToO) observation to scan the GW localization area for potential X-ray emission from the GW source. Although Insight-HXMT did not detect any significant high energy(0.2-5 MeV) radiation from GW170817, its observation helped to confirm the unexpected weak and soft nature of GRB 170817 A. Meanwhile,Insight-HXMT/HE provides one of the most stringent constraints(~10^(-7) to 10^(-6) erg/cm^2/s) for both GRB170817 A and any other possible precursor or extended emissions in 0.2-5 MeV, which help us to better understand the properties of EM radiation from this BNS merger. Therefore the observation of Insight-HXMT constitutes an important chapter in the full context of multi-wavelength and multi-messenger observation of this historical GW event. | TiPei Li ShaoLin Xiong ShuangNan Zhang FangJun Lu LiMing Song XueLei Cao Zhi Chang Gang Chen Li Chen TianXiang Chen Yong Chen YiBao Chen YuPeng Chen Wei Cui WeiWei Cui JingKang Deng YongWei Dong YuanYuan Du MinXue Fu GuanHua Gao He Gao Min Gao MingYu Ge YuDong Gu Ju Guan ChengCheng Guo DaWei Han Wei Hu Yue Huang Jia Huo ShuMei Jia LuHua Jiang WeiChun Jiang Jing Jin YongJie Jin Bing Li ChengKui Li Gang Li MaoShun Li Wei Li Xian Li XiaoBo Li XuFang Li YanGuo Li ZiJian Li ZhengWei Li XiaoHua Liang JinYuan Liao CongZhan Liu GuoQing Liu HongWei Liu ShaoZhen Liu XiaoJing Liu Yuan Liu YiNong Liu Bo Lu XueFeng Lu Tao Luo Xiang Ma Bin Meng Yi Nang JianYin Nie Ge OU JinLu Qu Na Sai Liang Sun Yin Tan Lian Tao WenHui Tao YouLi Tuo GuoFeng Wang HuanYu Wang Juan Wang WenShuai Wang YuSa Wang XiangYang Wen BoBing WU Mei Wu GuangCheng Xiao He Xu YuPeng Xu LinLi Yan JiaWei Yang Sheng Yang YanJi Yang AiMei Zhang ChunLei Zhang ChengMo Zhang Fan Zhang HongMei Zhang Juan Zhang Qiang Zhang Shu Zhang Tong Zhang Wei Zhang WanChang Zhang WenZhao Zhang Yi Zhang Yue Zhang YiFei Zhang YongJie Zhang Zhao Zhang ZiLiang Zhang HaiSheng Zhao JianLing Zhao XiaoFan Zhao ShiJie Zheng Yue Zhu YuXuan Zhu ChangLin Zou | 2018 | Science China(Physics,Mechanics & Astronomy)2018，61，3： | 12 |
| 7 | Clinical characteristics and prognosis of young patients with colorectal cancer in Eastern China显示文摘AIM:To explore the clinical characteristics and prognosis of young patients with colorectal cancer patients in Eastern China.METHODS:A total of 1335 patients with colorectal cancer treated from December 1985 to December 2005at the Second Affiliated Hospital of Zhejiang University School of Medicine were studied retrospectively.The patients were divided into two groups,a younger group(aged≤30 years)and an older group(aged>30 years),and comparison was made in the clinical characteristics and prognosis between the two groups.Chisquare test was used for data analysis of all categorical variables,and overall survival(OS)was calculated by the Kaplan-Meier method.A multivariate analysis was performed using the Cox model.RESULTS:There were 42(3.1%)and 1293(96.9%)cases in the younger group and older group,respectively.Univariate analysis showed that the 5-and10-year OS in the younger group were 33.9%and26.1%,respectively,and those in the older group were60.1%and 52.2%,respectively.Younger group had poor survival(χ2=14.146,P=0.000).Multivariate analysis revealed that age was not a dependent factor for prognosis(OR=0.866,95%CI:0.592-1.269,P=0.461).Stratified analysis indicated that in stageⅢandⅣdisease,the 5-and 10-year OS were 24.6%and14.8%in the younger group,and 40.4%and 33.3%in the older group,respectively,with a significant difference between the two groups(χ2=5.101,P=0.024).In the subgroup of radical surgery,the 5-and 10-year OS were 44.3%and 34.2%in the younger group,and69.6%and 60.5%in the older group,with a difference being significant between the two groups(χ2=7.830,P=0.005).CONCLUSION:Compared with older patients,the younger patients have lower survival,especially in the subgroups of stageⅢandⅣdisease and radical surgery. | Jian-Fei Fu Yan-Qin Huang Jiao Yang Cheng-Hao Yi Hai-Long Chen Shu Zheng | 2013 | World Journal of Gastroenterology2013，19，44： | 11 |
| 8 | Transcriptomic landscape regulated by the 14 types of bone morphogenetic proteins(BMPs)in lineage commitment and differentiation of mesenchymal stem cells(MSCs)显示文摘Mesenchymal stem cells(MSCs)are ubiquitously-existing multipotent progenitors that can self-renew and differentiate into multiple lineages including osteocytes,chondrocytes,adipocytes,tenocytes and myocytes.MSCs represent one of the most commonly-used adult progenitors and serve as excellent progenitor cell models for investigating lineagespecific differentiation regulated by various cellular signaling pathways,such as bone morphogenetic proteins(BMPs).As members of TGFb superfamily,BMPs play diverse and important roles in development and adult tissues.At least 14 BMPs have been identified in mammals.Different BMPs exert distinct but overlapping biological functions.Through a comprehensive analysis of 14 BMPs in MSCs,we demonstrated that BMP9 is one of the most potent BMPs in inducing osteogenic differentiation of MSCs.Nonetheless,a global mechanistic view of BMP signaling in regulating the proliferation and differentiation of MSCs remains to be fully elucidated.Here,we conducted a comprehensive transcriptomic profiling in the MSCs stimulated by 14 types of BMPs.Hierarchical clustering analysis classifies 14 BMPs into three subclusters:an osteo/chondrogenic/adipogenic cluster,a tenogenic cluster,and BMP3 cluster.We also demonstrate that six BMPs(e.g.,BMP2,BMP3,BMP4,BMP7,BMP8,and BMP9)can induce ISmads effectively,while BMP2,BMP3,BMP4,BMP7,and BMP11 up-regulate Smad-independent MAP kinase pathway.Furthermore,we show that many BMPs can upregulate the expression of the signal mediators of Wnt,Notch and PI3K/AKT/mTOR pathways.While the reported transcriptomic changes need to be further validated,our expression profiling represents the first-of-its-kind to interrogate a comprehensive transcriptomic landscape regulated by the 14 types of BMPs in MSCs. | Linghuan Zhang Qing Luo Yi Shu Zongyue Zeng Bo Huang Yixiao Feng Bo Zhang Xi Wang Yan Lei Zhenyu Ye Ling Zhao Daigui Cao Lijuan Yang Xian Chen Bin Liu William Wagstaff Russell R*Reid Hue H*Luu Rex C*Haydon Michael J*Lee Jennifer Moriatis Wolf Zhou Fu Tong-Chuan He Quan Kang | 2019 | Genes & Diseases2019，6，3： | 11 |
| 9 | A cross-sectional study on HGV infection in a rural population显示文摘AbstractAIMTodeterminetheepidemiologicalcharacteristicsandclinicalsignificanceofHGVinfection,andtocomparewithHBVandHCVinfecti... | LING Bin Hua 1, ZHUANG Hui 1, CUI Yi Hui 1, AN Wen Feng 1, LI Zhi Jie 1, WANG Shu Ping 2 and ZHU Wan Fu 2 | 1998 | World Journal of Gastroenterology1998，4，6： | 10 |
| 10 | The High Energy X-ray telescope (HE) onboard the Insight-HXMT astronomy satellite显示文摘The Insight-Hard X-ray Modulation Telescope(Insight-HXMT) is a broadband X-ray and γ-ray(1-3000 ke V) astronomy satellite. One of its three main telescopes is the High Energy X-ray telescope(HE). The main detector plane of HE comprises 18 Na I(Tl)/Cs I(Na) phoswich detectors, where Na I(Tl) is used as the primary detector to measure ~ 20-250 ke V photons incident from the field of view(FOV) defined by collimators, and Cs I(Na) is used as the active shielding detector to Na I(Tl) by pulse shape discrimination. Additionally, Cs I(Na) is used as an omnidirectional γ-ray monitor. The HE collimators have a diverse FOV,i.e. 1.1°×5.7°(15 units), 5.7°×5.7°(2 units), and blocked(1 unit). Therefore, the combined FOV of HE is approximately5.7°×5.7°. Each HE detector has a diameter of 190 mm resulting in a total geometrical area of approximately 5100 cm2, and the energy resolution is ~15% at 60 ke V. For each recorded X-ray event by HE, the timing accuracy is less than 10 μs and the deadtime is less than 10 μs. HE is used for observing spectra and temporal variability of X-ray sources in the 20-250 ke V band either by pointing observations for known sources or scanning observations to unveil new sources. Additionally, HE is used for monitoring the γ-ray burst in 0.2-3 Me V band. This paper not only presents the design and performance of HE instruments but also reports results of the on-ground calibration experiments. | CongZhan Liu YiFei Zhang XuFang Li XueFeng Lu Zhi Chang ZhengWei Li AiMei Zhang YongJie Jin HuiMing Yu Zhao Zhang MinXue Fu YiBao Chen JianFeng Ji YuPeng Xu JingKang Deng RenCheng Shang GuoQing Liu FangJun Lu ShuangNan Zhang YongWei Dong TiPei Li Mei Wu YanGuo Li HuanYu Wang BoBing Wu YongJie Zhang Zhi Zhang ShaoLin Xiong Yuan Liu Shu Zhang HongWei Liu YiJung Yang Fan Zhang | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，4： | 9 |
| 11 | A Facile Synthetic Route to L-Phosphinothricin显示文摘A convenient and effective method for synthesis of L-phosphinothricin was described.The highlight was involved in a simple access to the key intermediate L-2-amino-4-chlorobutyricacid derivative from the inexpensive L-methionine. | Xue Song XU Han Bing TENG Guo Fu QIU Shu Cai LIANG Xian Ming HU | 2006 | Chinese Chemical Letters2006，17，2： | 9 |
| 12 | Fourth C hinese National Consensus Report on the management of H elicobacter pylori infection显示文摘 | Wen Zhong Liu Yong Xie Hong Cheng Nong Hua Lu Fu Lian Hu Wan Dai Zhang Li Ya Zhou Ye Chen Zhi Rong Zeng Chong Wen Wang Shu Dong Xiao Guo Zong Pan Pin Jin Hu | 2013 | Journal of Digestive Diseases2013，，5： | 9 |
| 13 | Oncoprotein p28^GANK binds to RelA and retains NF-κB in the cytoplasm through nuclear export显示文摘 | Yao Chen Hong Hai Li Jing Fu Xue Feng Wang Yi Bin Ren Li Wei Dong Shan Hua Tang Shu Qing Liu Meng Chao Wu Hong Yang Wang | 2007 | Cell Research2007，17，12： | 9 |
| 14 | Management strategies of neonatal jaundice during the coronavirus disease 2019 outbreak显示文摘The outbreak of coronavirus disease 2019 (COVID-19;formally known as 2019-nCoV) has become a most challenging health emergency.Owing to rigorous quarantine and control measures taken in China,routine neonatal health surveillance and follow-up have become challenging.Without follow-up surveillance,some rapid and progressive newborn diseases,such as bilirubin encephalopathy,may be ignored.The characteristics of onset age of kernicterus suggest that monitoring of bilirubin level at home provides a useful way to alert hospital visits and to prevent the development of extremely hyperbilirubinemia.Therefore,we developed an online follow-up program for convenient monitoring of bilirubin level of newborns that is based on our practical experiences.The aim is to make our management strategies of neonatal jaundice tailored to the infection prevention and control during the COVID-19 epidemic. | Xiao-Lu Ma Zheng Chen Jia-Jun Zhu Xiao-Xia Shen Ming-Yuan Wu Li-Ping Shi Li-Zhong Du Jun-Fen Fu Qiang Shu | 2020 | World Journal of Pediatrics2020，16，3： | 8 |
| 15 | Finite Travelling Waves for a Semilinear Degenerate Reaction-Diffusion System | Shu WANG Cheng Fu WANG Dang LUO Department of Mathematics, Henan University, Kaifeng 475001, P. R. China Institute of Mathematics, Academy of Mathematics and System Sciences Chinese Academy of Sciences, Beijing 100080, P. R. China Department of Mathematics, Suzhou University, Suzhou 215006, P. R. China Department of Basic Science, North China Institute of Water Conservancy and Hydroelectric Power, Zhengzhou 450045, P. R. China | 2001 | Acta Mathematica Sinica,English Series2001，17，4： | 8 |
| 16 | Microstructures and mechanical properties of Nb-alloyed CoCrCuFeNi high-entropy alloys显示文摘Nb has a positive effect on improving the mechanical properties of metal materials, and it is expected to strengthen CoCrCuFeNi high-entropy alloys(HEAs) with outstanding ductility and relatively weak strength. In this paper, the alloying effects of Nb on the microstructural evolution and the mechanical properties of the(CoCrCuFeNi)_(100-x)Nb_xHEA were investigated systematically. The result shows that Nb promotes the phase transition from FCC(face-centered cubic) to Laves phase, and the volume fractions of Laves phase increase from 0% to 58.2% as the Nb content increases. Compressive testing shows that the addition of Nb has a positive effect on improving the strength of CoCrCuFeNi HEA. The compressive yield strength of(CoCrCuFeNi)_(100-x)Nb_xHEAs increases from 338 MPa to 1322 MPa and the fracture strain gradually reduces from 60.0%(no fracture) to 8.1% as the Nb content increases from 0 to 16 at.%. The volume fraction increase of hard Laves phase is the key factor for the strength increase, and the reduction of the VEC(valence electron concentration) value induced by the addition of Nb is beneficial for the increase of the Laves phase content in these alloys. | Gang Qin Shu Wang Ruirun Chen Xue Gong Liang Wang Yanqing Su Jingjie Guo Hengzhi Fu | 2018 | Journal of Materials Science & Technology2018，34，2： | 8 |
| 17 | A prospective study to evaluate the efficacy of an intracardiac electrogram-based atrioventricular and interventricular intervals optimization method in cardiac resynchronization therapy显示文摘有 biventricular 踱步的背景心脏的再同步治疗( CRT )与学习试图评估基于的 intracardiac 电记录图( IEGM )的功效的心 failure.The 电流改进在选择病人的生活的心脏的功能,功能的能力和质量优化方法, QuickOptTM ,在与 CRT.Methods 大动脉的时间对待的中国病人,速度积分( AVTI )完成了在察觉到心房与心室( AV ),在 AV 和两 Qu 推荐的 interventricular ( VV ) | Hua Wei Wang Dong-mei Cai Lin Sun Chao-feng Fu Guo-sheng Wang Yu-tang Yan Ji Luo Zhi-ling Xu Jing Wang Zhi-yong Xu Geng Shen Fa-rong Xu Wei Wang Jing-feng Ren Xue-jun Jin Wei Zhang, Nan Lau, Elizabeth Oi-Yan Zhang Shu | 2012 | Chinese Medical Journal2012，，3： | 8 |
| 18 | Fine mapping of GS2, a dominant gene for big grain rice显示文摘Grain shape as a major determinant of rice yield and quality is widely believed to be controlled by quantitative trait loci(QTL). We have identified a novel gene 'GS2' to largely regulate grain length and width in rice. The GS2 allele in the big-grain rice line ‘CDL’functioned in a dominant manner. In the present study, we employed a chromosome walking strategy in the residual heterozygous lines from recombinant inbred population between cultivar 'R1126' and CDL, and located the GS2 gene in an interval of ~33.2 kb flanked by marker GL2-35-1 and GL2-12 in the long arm of rice chromosome 2. According to genome annotations, three putative gene loci, LOC_Os02g47280, LOC_Os02g47290 and LOC_Os02g47300, exist in this candidate region. In addition, allelic analysis with previously reported genes demonstrated that GS2 was novel for regulating rice grain shape. These results will help promote the cloning and functional characterization of the GS2 gene and further develop linked markers to be used in marker-assisted breeding. | Wuhan Zhang Pingyong Sun Qiang He Fu Shu Jie Wang Huafeng Deng | 2013 | The Crop Journal2013，1，2： | 7 |
| 19 | An experimental study on astrocytes promoting production of neural stem cells derived from mouse embryonic stem cells显示文摘Background The production of neural stem cells (NSCs) derived from embryonic stem (ES) cells was usually very low according to previous studies, which was a major obstacle for meeting the needs of clinical application. This study aimed at investigating whether astrocytes could promote production of NSCs derived from ES cells in vitro.Methods Mouse ES cells line-D3 was used to differentiate into NSCs with astrocytes as inducing stromal cells by means of three-stage differentiation procedure. Another group without astrocytes served as control. The totipotency of ES cells was identified by observation of cells’ morphology and formation of teratoma in severe combined immunodeficiency disease (SCID) mice. The quantity and purity of NSCs derived from ES cells were analyzed using clonogenic assay, immunohistochemical staining and flow cytometry assay. The plasticity of NSCs was detected by differentiating test. Octamer-binding transcription factor 4 (Oct-4) and nestin, the specific marker genes of ES cells and NSCs respectively, were detected continuously using reverse transcription-polymerase chain reaction (RT-PCR) method to monitor the process of cell differentiation.Results The ES cells of D3 line could maintain the ability of differentiating into cellular derivations of all three primary germ layers after continuous passage culture. At the end of two-stage of inducing process, 23.2±3.5 neurospheres per plate formed in astrocyte-induced group and only 0.8±0.3 per plate in the control group (clonogenic assay, P<0.01), and the ratio of nestin positive cells was (50.2±2.8)% in astrocyte-induced group and only (1.4±0.5)% in the control group (flow cytometry, P<0.01). With the induction undergoing, the expression of Oct-4 gradually decreased and then disappeared, while the expression of nestin was increased step by step, and the ratio of nestin positive cells was up to 91.4% by the three-stage differentiation. The nestin positive cells could be further induced into neurons, astrocytes, and oligodendrocytes in differentiating medium supplemented with fetal calf serum. The results of differentiating test showed that the ratio of NF-200 and NSE positive cells was (42.7±2.6)% in astrocyte-induced group and only (11.2±1.8)% in the control group (P<0.01).Conclusions Astrocytes can not only increase the production of NSCs derived from ES cells but also promote the differentiation of NSCs toward neuronal lineage. | ZHOU Yu-feng FANG Feng FU Jin-rong DONG Yong-sui YE Du-yun SHU Sai-nan ZHEN Hong LI Ge | 2005 | Chinese Medical Journal2005，，23： | 7 |
| 20 | Systematic Identification of the Light-quality Responding Anthocyanin Synthesis-related Transcripts in Petunia Petals显示文摘Previous studies have shown that high light intensity can induce anthocyanin synthesis(AS)in petunia plants.To identifywhich kind of light quality plays a role in inducing such metabolic process,and what transcripts participate in controlling it,we carried out whole-transcriptome sequencing and analysis of petunia petals treated with different light-quality conditions.Among the red and white light treatments,a total of 2205 differentially expressed genes and 15,22,and 20 differentially expressed circRNAs,miRNAs,and lncRNAs,were identified respectively.The AS-related genes,including the structural genes CHSj,F3H,F35H,DFR,and ANS,and the regulatory genes AN4,DPL,PHZ and MYBx were found to be downregulated under red light condition compared with their levels under white light condition.Furthermore,the light photoreceptor Cryptochrome 3(CRY3)and a series of light-dependent genes,such as PIF,HY5,andBBXs,were also determined to respond to the light treatments.The anthocyanin contents in early petunia petals under red light were significantly lower than that under white and blue light.The results of qRT-PCR further confirmed the expression pattern of some AS-related and light-response genes in response to different light quality.Yeast two-hybrid results showed that the key elements in the light signal pathway,HY5 can interact with BBX19,BBX24 and BBX25.And PHZ,the important AS regulator can induce anthocyanin synthesis in response to blue light quality fromtransient expression analysis in petunia petals.These findings presented here not only deepen our understanding of how light quality controls anthocyanin synthesis,but also allow us to explore potential target genes for improving pigment production in petunia flower petals. | Zhenzhu Fu Hongquan Shang Hui Jiang Jie Gao Xiaoyu Dong Huijuan Wang Yanmin Li Limin Wang Jing Zhang Qingyan Shu Yacong Chao Menglan Xu Rui Wang Liangsheng Wang Hechen Zhang | 2020 | Horticultural Plant Journal2020，6，6： | 7 |
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