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| 1 | A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine显示文摘Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to crossing.Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery.To bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision making.The results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine breeding.The vast majority of sequence reads(⩾99%)were from the targeted regions.Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis. | Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks Anna Katharine Mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | ZD1839 ('IRESSA ') A selective epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI):inhibition of c-fos mRNA,an intermediate marker of EGFR activation,correlates with tumor growth inhibition显示文摘 | Woodburn JR Kendrew J Fennell M | 2000 | Proceedings of the American Association for Cancer Research2000，，： | 1 |
| 3 | Multilevel research and the challenges of implementing genomic medicine 显示文摘 | Khoury M J Coates R J Fennell ML | 2012 | J Natl Cancer Inst Monogr2012，2012，44： | 1 |
| 4 | Quantification of penicillinG and procaine in equine urine and plasma using high-performance liquid chromatography显示文摘 | Luo Y McNamara B Fennell M A | 1998 | J Chromatogr B1998，714，2： | 1 |
| 5 | Leadership instability in hospitals:The influence of board-CEO relations and organizational growth and decline显示文摘 | Alexander J A Fennell M L Halpern M T | | 0，，01： | 1 |
| 6 | Plant regeneration from immature embryo of 48 elite CIMMYT bread wheat显示文摘 | FENNELL S N BOHOROVA M VAN G J | 1996 | Theor Appl Genet1996，92，： | 1 |
| 7 | Testing a marine ecosystem model:sensitivity analysis and parameter optimization显示文摘 | Fennel K Losch M Schroter J | 2001 | Journal of Marine Systems2001，28，12： | 1 |
| 8 | New point of care test is highly specific but less sensitive for influenza virus A and B in children and adults显示文摘 | Rawlinson WD WaIiuzzaman ZM Fennell M | 2004 | J Med Virol2004，74，1： | 1 |
| 9 | Heterogeneous photocatalysis for control of volatile organic compounds in indoor air 显示文摘 | Jacoby W A D M Blake J A Fennell ct al | 1996 | Air & Waste Manage1996，46，9： | 1 |
| 10 | Targeting JNK3 for the treatment of neurodegenerative disorders 显示文摘 | RESNICK L FENNELL M | 2004 | Drug Discov Today2004，9，21： | 1 |
| 11 | Benign calcaneal bone cyst and pathologic fracture-surgical treatment with injectable calcium-phosphate bone cement ( Norian ): a case report 显示文摘 | Csizy M Buckley RE Fennell C | 2001 | Foot Ankle Iht2001，22，6： | 1 |
| 12 | Plant regeneration from immature embryos of 48 elite CIMMYT bread wheats 显示文摘 | Fennel S Bohorova N Van Ginkel M | 1996 | Theor Appl Genet1996，92，： | 1 |
| 13 | Oxidative stress and vas- cular damage in hypertension 显示文摘 | Berry C Brosman M J Fennell J | 2001 | Curr Opin Nephrol Hypertens2001，10，： | 1 |
| 14 | Multiparameter measurement of caspase 3 activation and apoptotic cell death in NT2 neuronal precursor cells using high-content analysis显示文摘 | Fennell M Chan H Wood A | 2006 | J Biomol Screen2006，11，3： | 1 |
| 15 | Screening of immunophilin ligands by quantitative analysis of neurofilament expression and neurite outgrowth in cultured neurons and cells 显示文摘 | Liu D Mcllvain H B Fennell M | 2007 | Neurosei Methods2007，163，2： | 1 |
| 16 | In vivo suppression of Bcl-XL expression facilitates chemotherapy-induced leukaemia cell death in a SCID/NOD-Hu model显示文摘 | Fennell DA Corbo M V Dean NM | 2001 | Br J Haematol2001，112，： | 1 |
| 17 | Heterogeneous photocatalysis for control of volatile organic compounds in indoor air 显示文摘 | Jacoby W A Blake D M Fennell J A | 1996 | Journal of the Air & Waste Management Association1996，，： | 1 |
| 18 | Internal Charging: a Preliminary Environmental Specification for Satellites显示文摘 | FENNELL J F KOONS H C CI-IEN M W | 2000 | Ieee Trans Plasma Sci2000，28，6： | 1 |
| 19 | Comparison of butyric acid, ethanol, lactic acid, and propionic acid as hydrogen donors for the reductive dechlorination of tetrachloroethene 显示文摘 | Fennell D E Gossett J M Zinder S H | 1997 | Environ Sci Technol1997，31，： | 1 |
| 20 | Testing a marine ecosystem model: sensitivity analysis and parameter optimization显示文摘 | Fennel K Losch M Schroter J | 2001 | J Mar Syst2001，28，： | 1 |
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