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| 1 | Intravenous vs intraperitoneal mesenchymal stem cells administration:What is the best route for treating experimental colitis?显示文摘AIM:To investigate the therapeutic effects of mesenchymal stem cells(MSCs)transplanted intraperitoneally andintravenously in a murine model of colitis.METHODS:MSCs were isolated from C57BL/6 mouse adipose tissue.MSC cultures were analyzed according to morphology,cellular differentiation potential,and surface molecular markers.Experimental acute colitis was induced in C57BL/6 mice by oral administration of2%dextran sulfate sodium(DSS)in drinking water ad libitum from days 0 to 7.Colitis mice were treated with1×106 MSCs via intraperitoneal or intravenous injection on days 2 and 5.The disease activity index was determined daily based on the following parameters:weight loss,stool consistency and presence of blood in the feces and anus.To compare morphological and functional differences in tissue regeneration between different MSC injection modalities,mice were euthanized on day 8,and their colons were examined for length,weight,and histopathological changes.Inflammatory responses were determined by measuring the levels of different serum cytokines using a CBA Th1/Th2/Th17 kit.Apoptotic rates were evaluated by terminal deoxynucleotidyl transferase-mediated d UDPbiotin nick end labeling assay.RESULTS:Intravenous infusion of MSCs was more effective than intraperitoneal treatment(P<0.001)in reducing the clinical and histopathologic severity of colitis,which includes weight loss,diarrhea and inflammation.An histological evaluation demonstrated decreased colonic inflammation based on reduced crypt loss and reduced infiltration of inflammatory cells.This therapeutic effect was most likely mediated by the down-regulation of pro-inflammatory cytokines[interleukin(IL)-6 and tumor necrosis factor(TNF)];and by the up-regulation of anti-inflammatory cytokines(IL-10 and IL-4).Intravenous transplantation alsoinduced high levels of IFN that lead to activation of the immunosuppressive activity of the MSCs,which did not occur with intraperitoneal transplantation(P=0.006).An increase in apoptotic T cells was observed after intravenous,but not intraperitoneal,MSC infusion,suggesting that MSCs can induce apoptosis in resistant T cells in colonic inflammation(P=0.027).CONCLUSION:Our results demonstrate that intravenous treatment is a superior method for reducing colon inflammation compared with intraperitoneal therapy. | Fabiany da Costa Goncalves Natália Schneider Fernanda Otesbelgue Pinto Fabíola Schons Meyer Fernanda Visioli Bianca Pfaffenseller Patrícia Luciana da Costa Lopez Eduardo Pandolfi Passos Elizabeth Obino Cirne-Lima Luíse Meurer Ana Helena Paz | 2014 | World Journal of Gastroenterology2014，20，48： | 4 |
| 2 | A genetic linkage map for zebrafish: comparative analysis and localization of genes and expressed sequences 显示文摘 | Michael AG Lisa K Elizabeth SE | 1999 | Gen Res1999，9，4： | 1 |
| 3 | Molecular control of vertebrate iron homeostasis by iron regulatory proteins 显示文摘 | Michelle LW Elizabeth AL Richard SE | 2006 | Biochimica et Biophysica Acta2006，1763，7： | 1 |
| 4 | Transfection of mammalian cells using linear polyethylenimine is a simple and effective means of producing recombinant adeno-associated virus vectors显示文摘 | Reed SE Staley EM Elizabeth M | | 0，，1： | 1 |
| 5 | Genome-wide siRNA screen Identifies SMCX,EP400 and Brd4 as E2-depend- ent regulators of human papillomavirus oncogene expression 显示文摘 | Jennifer SA Elizabeth WA Mathew SE | 2010 | Pnas2010，107，8： | 1 |
| 6 | Kegel Exercises显示文摘 | Katharine Kolcaba Thérèse Dowd Elizabeth H. Winslow Ann F. Jacobson | 2000 | American Journal of Nursing2000，，11： | 1 |
| 7 | Ontogeny oforientation flight in the honeybee revealed by harmonic radar显示文摘 | Elizabeth AC Smith AD Osborne JL Fahrbach SE Farris SM Reynolds DR Edwards AS Martin A Robinson GE Poppy GM Riley JR | | 0，，： | 1 |
| 8 | Peptides on phage: a vast library of peptides for identifying ligands显示文摘 | Cwirla SE Elizabeth AP Barrett RW | 1990 | Proc Natl Acad Sci USA1990，87，： | 1 |
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