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| 1 | Dynamic tracking of stem cells in an acute liver failure model显示文摘AIM:To investigate a dual labeling technique,which would enable real-time monitoring of transplanted embryonic stem cell(ESC) kinetics,as well as long-term tracking.METHODS:Liver damage was induced in C57/BL6 male mice(n = 40) by acetaminophen(APAP) 300 mg/kg administered intraperitoneally.Green fluorescence protein(GFP) positive C57/BL6 mouse ESCs were stained with the near-infrared fluorescent lipophilic tracer 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide(DiR) immediately before transplantationinto the spleen.Each of the animals in the cell therapy group(n = 20) received 5 × 10 6 ESCs 4 h following treatment with APAP.The control group(n = 20) received the vehicle only.The distribution and dynamics of the cells were monitored in real-time with the IVIS Lumina-2 at 30 min post transplantation,then at 3,12,24,48 and 72 h,and after one and 2 wk.Immunohistochemical examination of liver tissue was used to identify expression of GFP and albumin.Plasma alanine aminotransferase(ALT) was measured as an indication of liver damage.RESULTS:DiR-stained ESCs were easily tracked with the IVIS using the indocyanine green filter due to its high background passband with minimal background autofluorescence.The transplanted cells were confined inside the spleen at 30 min post-transplantation,gradually moved into the splenic vein,and were detectable in parts of the liver at the 3 h time-point.Within 24 h of transplantation,homing of almost 90% of cells was confirmed in the liver.On day three,however,the DiR signal started to fade out,and ex vivo IVIS imaging of different organs allowed signal detection at time-points when the signal could not be detected by in vivo imaging,and confirmed that the highest photon emission was in the liver(P < 0.0001).At 2 wk,the DiRsignal was no longer detectable in vivo ;however,immunohistochemistry analysis of constitutively-expressed GFP was used to provide an insight into the distribution of the cells.GFP +ve cells were detected in tissue sections resembling hepatocytes and were dispersed throughout the hepatic parenchyma,with the presence of a larger number of GFP +ve cells incorporated within the sinusoidal endothelial lining.Very faint albumin expression was detected in the transplanted GFP +ve cells at 72 h;however at 2 wk,few cells that were positive for GFP were also strongly positive for albumin.There was a significant improvement in serum levels of ALT,albumin and bilirubin in both groups at 2 wk when compared with the 72 h time-point.In the cell therapy group,serum ALT was significantly(P = 0.016) lower and albumin(P = 0.009) was significantly higher when compared with the control group at the 2 wk time-point;however there was no difference in mortality between the two groups.CONCLUSION:Dual labeling is an easy to use and cheap method for longitudinal monitoring of distribution,survival and engraftment of transplanted cells,and could be used for cell therapy models. | Tarek Ezzat Dipok Kumar Dhar Massimo Malago Steven WM Olde Damink | 2012 | World Journal of Gastroenterology2012，18，6： | 12 |
| 2 | Over - expression of macrophage migration inhibitory factor induces angiogenesis and deteriorates prognosis after radical resection for hepatocellular carcinoma 显示文摘 | Eiji H Takashi O Dipok KD | 2005 | Cancer2005，103，3： | 1 |
| 3 | Long-term survival of transmural advanced gastric carcinoma following curative resection:multivariate analysis of prognostic factors显示文摘 | Dipok K Hirofumi K Mitsuo T | 2000 | World J Surg2000，24，5： | 1 |
| 4 | Cyclooxygenase-2 expression correlates with tumor neovascularization and prognosis in human colorectal carcinoma patients显示文摘 | REIKO M HITOSHI K DIPOK KD | 2000 | Cli Can Res2000，6，10： | 1 |
| 5 | Locling as acetylation cata- lyst in the preparation of 1,1 - diacetates from aldehydes 显示文摘 | NABBJYOTI D DIPOK J K RULI B | 1997 | Org Chem1997，62，3： | 1 |
| 6 | Importance of Connexin-43 based gap junction in cirrhosis and acute-on-chronic liver failure显示文摘 | Vairappan Balasubramaniyan Dipok Kumar Dhar Anne E. Warner Wai-Yin Vivien Li Azin Farzan Amiri Beverley Bright Rajeshwar P. Mookerjee Nathan A. Davies David L. Becker Rajiv Jalan | 2013 | Journal of Hepatology2013，，： | 1 |
| 7 | Prevention of acute kidney injury in a rodent model of cirrhosis following selective gut decontamination is associated with reduced renal TLR4 expression显示文摘 | Naina Shah Dipok Dhar Fatma El Zahraa Mohammed Abeba Habtesion Nathan A. Davies Maria Jover-Cobos Jane Macnaughtan Vikram Sharma Steven W.M. Olde Damink Rajeshwar P. Mookerjee Rajiv Jalan | 2012 | Journal of Hepatology2012，，5： | 1 |
| 8 | Expression of Trefoil Factor Family Members Correlates with Patient Prognosis and Neoangiogenesis显示文摘 | Dipok Kumar Dhar Timothy C Wang Hideki Tabara | 2005 | Clin Cancer Res2005，11，： | 1 |
| 9 | Expression of regenerating gene I in gastric adenocarcinomas显示文摘 | Dipok KD Udagawa J Ishihara S | 2004 | Cancer2004，100，6： | 1 |
| 10 | Impact of screening survey of gastric cancer on clinicopathological features and survival: Retrospective study at a single institution显示文摘 | Hirofumi Kubota Tsukasa Kotoh Reiko Masunaga Dipok Kumar Dhar Muneaki Shibakita Mitsuo Tachibana Hitoshi Kohno Naofumi Nagasue | 2000 | Surgery2000，，1： | 1 |
| 11 | Clinical outcomes of extended esophagectomy with three-field lymph node dissection for esophageal squamous cell carcinoma显示文摘 | Mitsuo Tachibana Shoichi Kinugasa Hiroshi Yoshimura Muneaki Shibakita Yasuhito Tonomoto Dipok Kumar Dhar Naofumi Nagasue | 2005 | The American Journal of Surgery2005，，1： | 1 |
| 12 | Expression of regenerating gene I in gastric adenocarcinomas显示文摘 | Dipok K D Udagawa S Ishihara | 2004 | Cancer2004，100，： | 1 |
| 13 | A tailored perioperative blood transfusion might avoid undue recurrences in gastric carcinoma patients显示文摘 | Dipok KD Hirofumi K | 2000 | Digestive Disases and Sciences2000，45，9： | 1 |
| 14 | Over expression of macrophage migration inhibitory facfor induces angiogenesis and dete-riorates prognosis after radical resection for hepato-cellular carcinoma显示文摘 | Eiji H Takashi O Dipok K D | 2005 | Cancer2005，103，： | 1 |
| 15 | Prognostic impact of hypoxia-inducible factors 1 and 2 in colorectal cancer patients:correlation with tumor angiogenesis and cyclooxygenase-2 expression显示文摘 | Hiroshi Y Dipok KD Hitoshi Kohno | 2004 | Clin Cancer Res2004，10，24： | 1 |
| 16 | Over expression of macro-phage migration inhibitory facfor induces angiogenesis anddete-riorates prognosis after radical resection for hepato-cellular carcinoma显示文摘 | Eiji H Takashi O Dipok KD | 2005 | Cancer2005，103，3： | 1 |
| 17 | Over expression of macro-phage migration inhibitory facfor induces angiogenesis and dete-riorates prognosis after radical resection for hepatocellular carcinoma 显示文摘 | Eiji H Takashi O Dipok KD | 2005 | Cancer2005，103，3： | 1 |
| 18 | Prognostic significance of fas and fas ligand expressions in human esophageal cancer显示文摘 | Mitsuo T Dipok KD | 1999 | Clin Cancer Res1999，5，9： | 1 |
| 19 | Appraisal of a revised lymph node classification system for esophageal squamous cell cancer显示文摘 | Dipok Kumar Dhar Shinji Hattori Yasuhito Tonomoto | 2007 | Ann Thorac Surg2007，83，： | 1 |
| 20 | Over expression of macrophage migration inhibitory factor induces angiogenesis and deteriorates prognosis after radical resection for hepatocelular carcinoma显示文摘 | Eiji H Takashi O Dipok KD | 2005 | Cancer2005，103，8： | 1 |
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