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| 1 | A dual positional specific lipoxygenase functions in the generation of flavor compounds during climacteric ripening of apple显示文摘Lipoxygenase(LOX)is an important contributor to the formation of aroma-active C6 aldehydes in apple(Malus×domestica)fruit upon tissue disruption but little is known about its role in autonomously produced aroma volatiles from intact tissue.We explored the expression of 22 putative LOX genes in apple throughout ripening,but only six LOXs were expressed in a ripening-dependent manner.Recombinant LOX1:Md:1a,LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b proteins showed 13/9-LOX,9-LOX,13/9-LOX and 13-LOX activity with linoleic acid,respectively.While products of LOX1:Md:1c and LOX2:Md:2b were S-configured,LOX1:Md:1a and LOX2:Md:2a formed 13(R)-hydroperoxides as major products.Site-directed mutagenesis of Gly567 to an alanine converted the dual positional specific LOX1:Md:1a to an enzyme with a high specificity for 9(S)-hydroperoxide formation.The high expression level of the corresponding MdLOX1a gene in stored apple fruit,the genetic association with a quantitative trait locus for fruit ester and the remarkable agreement in regio-and stereoselectivity of the LOX1:Md:1a reaction with the overall LOX activity found in mature apple fruits,suggest a major physiological function of LOX1:Md:1a during climacteric ripening of apples.While LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b may contribute to aldehyde production in immature fruit upon cell disruption our results furnish additional evidence that LOX1:Md:1a probably regulates the availability of precursors for ester production in intact fruit tissue. | Doreen Schiller Carolina Contreras Jörg Vogt Frank Dunemann Bruno G Defilippi Randolph Beaudry Wilfried Schwab | 2015 | Horticulture Research2015，2，1： | 6 |
| 2 | Nonalcoholic fatty liver disease and serum lipoproteins: The Multi-Ethnic Study of Atherosclerosis显示文摘 | Andrew Paul DeFilippis Michael J. Blaha Seth S. Martin Robert M. Reed Steven R. Jones Khurram Nasir Roger S. Blumenthal Matthew J. Budoff | 2013 | Atherosclerosis2013，，： | 2 |
| 3 | Altered Small Bowel Motility in Patients with Liver Cirrhosis Depends on Severity of Liver Disease显示文摘 | Ana Maria Madrid Francisco Cumsille Carlos Defilippi | 1997 | Digestive Diseases and Sciences1997，，4： | 2 |
| 4 | Epidemiology and clinical features of Mycoplasma pneumoniae infection in children显示文摘 | Annacarla Defilippi Michela Silvestri Angela Tacchella Raffaella Giacchino Giovanni Melioli Eddi Di Marco Carmela Cirillo Pasquale Di Pietro Giovanni A. Rossi | 2008 | Respiratory Medicine2008，，12： | 2 |
| 5 | Epidemiology and clinical features of mycoplasma pneumoniae infection in children显示文摘 | DEFILIPPI A SILVESTRI M TACCHELLA A | | Respir Med0，102，12： | 1 |
| 6 | Understanding amino-ter- minal pro-B-type natriuretic peptide in obesity 显示文摘 | Bayes-Genis A DeFilippi C Januzzi JL | 2008 | Am J Cardiol2008，101，： | 1 |
| 7 | N-terminal pro-Btype- natriuretic peptide for predicting coronary disease andleft ven- trieular hypertrophy in asymptomatic CKD not requiring dial- ysis显示文摘 | DeFilippi CR Fink JC Nass CM | 2005 | Am J Kidney Dis2005，46，1： | 1 |
| 8 | Development and control of scald on wonderful pomegranates during long- term storage显示文摘 | Defilippi BG Whhitaker BD Pierce BMH | 2006 | Postharvest Biology and Technology2006，41，3： | 1 |
| 9 | Chemo-immunotherapy in advanced head and neck cancer 显示文摘 | Recchia F Sica G DeFilippis S | 1999 | Anticancer Res1999，19，1： | 1 |
| 10 | Pulmonary irradiation-induced expression of VCAM-Ⅰ and ICAM-Ⅰ is decreased by manganese superoxide dismutase-plasmid/liposome(MnSOD-PL) gene therapy显示文摘 | Epperly MW Sikora CA DeFilippi SJ | 2002 | Biol Blood Marrow Transplant2002，8，： | 1 |
| 11 | Phase Ⅰ-Ⅱ study of dose intensified chemotherapy with filgrastimand thymopentin in patients with advanced cancer 显示文摘 | Recchia F Defilippis S Ginaldi L | 1997 | Clin Ter1997，148，56： | 1 |
| 12 | CD36 repression activates a muhicultural stoma program shared by high mammo-graphic density and tumor tissues 显示文摘 | Defilippis RA Chang H Dumont N | 2012 | Cancer Discover2012，2，9： | 1 |
| 13 | Amino-terminal pro- B-type natriuretic peptide testing in renal disease显示文摘 | deFilippi CR van Kimmenade RR Pinto YM | 2008 | Am J Cardiol2008，101，： | 1 |
| 14 | Effect of down-regulation of ethylene biosynthesis on fruit flavor complex in apple fruit显示文摘 | Dandekar A M Defilippi B G Uratsu S L | 2004 | Transgenic Research2004，13，4： | 1 |
| 15 | N-terminal pro-B type natriuretic peptide fur predicting coronary disease and left ventricular hypertrophy in asymptomatic CKD not requiring dialysis显示文摘 | DeFilippi CR Fink JC Nass CM | 2005 | Am J Kidney Dis2005，46，1： | 1 |
| 16 | Apple aroma:alcohol acyltransferase, a rate limiting step for ester biosynthesis,is regulated by erbylene显示文摘 | DEFILIPPI B G KADER A A DANDEKA A M | 2005 | Plant Science2005，，168： | 1 |
| 17 | Effect of down- regulation of ethylene biosynthesis on fruit flavor complex in apple fruit显示文摘 | DANDEKAR A M TEO G DEFILIPPI B G URATSU S L PASSEY A J KADER A A STOW J R COLGAN R J JAMES D J | 2004 | Transgenic Research2004，13，： | 1 |
| 18 | Role of the retinoblastoma pathway in senescence triggered by repression of the human papillomavirus E7 protein in cervical carcinoma cells显示文摘 | Amanda Psyrril Rosa Anna DeFilippis Anne PB | 2004 | Cancer Res2004，64，9： | 1 |
| 19 | Cytomegalovirus immune evasion显示文摘 | Powers C DeFilippis V Malouli D Früh K | 2008 | Curr Top Microbiol Immunol2008，325，： | 1 |
| 20 | Sta- tin therapy is associated with decreased pulmonary vascular pressures in severe COPD显示文摘 | REED R M IACONO A DEFILIPPIS A | 2011 | COPD2011，8，： | 1 |
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