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| 1 | Effect of six kinds of scale inhibitors on calcium carbonate precipitation in high salinity wastewater at high temperatures显示文摘Precipitation of calcium carbonate(Ca CO3) scale on heat transfer surfaces is a serious and expensive problem widely occurring in numerous industrial processes. In this study, we compared the scale inhibition effect of six kinds of commercial scale inhibitors and screened out the best one(scale inhibitor SQ-1211) to investigate its scale inhibition performance in highly saline conditions at high temperature through static scale inhibition tests. The influences of scale inhibitor dosage, temperature, heating time and p H on the inhibition efficiency of the optimal scale inhibitor were investigated. The morphologies and crystal structures of the precipitates were characterized by Scanning Electron Microscopy and X-ray Diffraction analysis. Results showed that the scale inhibition efficiency of the optimal scale inhibitor decreased with the increase of the reaction temperature. When the concentration of Ca2+was 1600 mg/L, the scale inhibition rate could reach 90.7% at 80°C at p H 8. The optimal scale inhibitor could effectively retard scaling at high temperature. In the presence of the optimal scale inhibitor, the main crystal structure of Ca CO3 changed from calcite to aragonite. | Xiaochen Li Baoyu Gao Qinyan Yue Defang Ma Hongyan Rong Pin Zhao Pengyou Teng | 2015 | Journal of Environmental Sciences2015，27，3： | 15 |
| 2 | Effect of Sanhuangwuji powder,anti-rheumatic drugs,and ginger-partitioned acupoint stimulation on the treatment of rheumatoid arthritis with peptic ulcer:a randomized controlled study显示文摘OBjECTIVE:To observe the efficacy and safety of oral Sanhuangwuji powder,anti-rheumatic drugs(ARDs),and ginger-partitioned acupoint stimulation at Zusanli(ST 36) on the treatment of rheumatoid arthritis(RA) complicated by peptic ulcer.METHODS:This prospective randomized controlled study included 180 eligible inpatients and outpatients randomly assigned to an ARD treatment(n = 60),ginger-partitioned stimulation(n =60),or combination treatment(n = 60).Patients assigned to the ARD group were given oral celecoxib,methotrexate,and esomeprazole.Patients assigned to theginger-partitioned stimulation group were given ginger-partitioned acupoint stimulation at Zusanli(ST 36) in addition to the ARDs.Patients in the combination treatment group were given oral Sanhuangwuji powder,ginger-partitioned acupoint stimulation at Zusanli(ST 36),and ARDs.All patients were followed up for 2 months to evaluate clinical effects and safety.The study was registered in the World Health Organization database at the General Hospital of Chengdu Military Area Command Chinese People's Liberation Army(ChiCTR-TCC12002824).RESULTS:The combination treatment group had significantly greater improvements in RA symptoms,laboratory outcomes,and gastrointestinal symptom scores,compared with the other groups(P < 0.05).The peptic ulcer healing rate in the combination treatment group was significantly greater than that in the ARD treatment group(x^2 = 16.875,P < 0.05) and the ginger-partitioned stimulation group(x^2 = 6.171,P<0.05).CONCLUSION:Combination treatment with ginger-partitioned acupoint stimulation at Zusanli(ST36),oral Sanhuangwuji powder,and ARDs had a better clinical effect for RA with complicated peptic ulcer,compared with ARD treatmentalone or in combination with ginger-partitioned acupoint stimulation. | Liu Defang Guo Mingyang Hu Yonghe Liu Taihua Yan Jiao Luo Yong Yun Mingdong Yang Min Zhang Jun Guo Linglin | 2015 | Journal of Traditional Chinese Medicine2015，35，3： | 9 |
| 3 | Predicting oral disintegrating tablet formulations by neural network techniques显示文摘Oral disintegrating tablets(ODTs) are a novel dosage form that can be dissolved on thetongue within 3 min or less especially for geriatric and pediatric patients. Current ODT for-mulation studies usually rely on the personal experience of pharmaceutical experts andtrial-and-error in the laboratory, which is inefficient and time-consuming. The aim of cur-rent research was to establish the prediction model of ODT formulations with direct com-pression process by artificial neural network(ANN) and deep neural network(DNN) tech-niques. 145 formulation data were extracted from Web of Science. All datasets were dividedinto three parts: training set(105 data), validation set(20) and testing set(20). ANN andDNN were compared for the prediction of the disintegrating time. The accuracy of the ANNmodel have reached 85.60%, 80.00% and 75.00% on the training set, validation set and testingset respectively, whereas that of the DNN model were 85.60%, 85.00% and 80.00%, respec-tively. Compared with the ANN, DNN showed the better prediction for ODT formulations.It is the first time that deep neural network with the improved dataset selection algorithmis applied to formulation prediction on small data. The proposed predictive approach couldevaluate the critical parameters about quality control of formulation, and guide researchand process development. The implementation of this prediction model could effectivelyreduce drug product development timeline and material usage, and proactively facilitatethe development of a robust drug product. | Run Han Yilong Yang Xiaoshan Li Defang Ouyang | 2018 | Asian Journal of Pharmaceutical Sciences2018，13，4： | 6 |
| 4 | Multi-layered micro/nanofibrous nonwovens for functional face mask filter显示文摘The worldwide COVID-19 pandemic has led to an attention on the usage of personal protective face masks.However,the longevity and safety of the commercial face masks are limited due to the charge dissipation of the electret meltblown nonwovens,which are dominate in the face mask filters.Herein,we design a type of multi-layer structured nonwovens using meltblowing and electrospinning technologies.The complex nonwovens involving meltblown and electrospun fibers are designed to possess multilevel fiber diameters and pore sizes.The micro/nanofibers with porous and wrinkled surface morphologies can well capture particulate matters(PMs),and the multilevel pore sizes contribute to low air resistance under high filtration efficiency.Airflow field simulation was carried out to understand the pressure distribution within the nonwovens in the filtration process.Meanwhile,by adding Ag nanoparticles(AgNPs)as additives,the nonwovens exhibit excellent antibacterial performance.The resultant nonwovens exhibit filtration efficiency of 99.1%for PM0.3 and low pressure drop of 105 Pa under the 10.67 cm/s inlet air velocity,and antibacterial rate of>99.99%for Escherichia coli.These performances and functions make the designed complex nonwovens a promising filter core for face masks. | Yuanqiang Xu Xiaomin Zhang Defang Teng Tienan Zhao Ying Li Yongchun Zeng | 2022 | Nano Research2022，15，8： | 6 |
| 5 | CD13/APN expression in peripheral blood lymphocytes and skin lesions in patients with advanced psoriasis vulgaris显示文摘Objective: To observe the expression of CD13/APN in peripheral blood lymphocytes and skin lesions of patients with advanced psoriasis vulgaris, and discuss its effect on the pathogenesis of psoriasis. Methods: CD13 expression in peripheral blood lymphocytes and skin lesions was detected by flow cytometry and immunohistochemical technique, respectively. Results were compared with those of healthy controls. Results: CD13 expression was significantly higher in peripheral blood lymphocytes of patients with advanced psoriasis vulgaris than in that of healthy controls, and in skin lesions than in healthy skin tissues. The expression was mainly in the suprabasal layers of skin lesions, and positively correlated to PASI (R=0.78029). Conclusion: The significantly higher expression of CD13 in peripheral blood lymphocytes and skin lesions of the patients with advanced psoriasis vulgaris probably is related to immunological abnormality, blood vessel abnormality and proliferation of keratinocyte in the pathogenic course of psoriasis. It may be a novel and effective way to treat psoriasis with specific CD13 inhibitors. | Liu Taihua Liu Defang Chen Yihua Hu Zonghai Chen Lu Luo Chen Xu Zhejuan | 2009 | Journal of Medical Colleges of PLA(China)2009，24，1： | 5 |
| 6 | Discovery of Mass Independent Oxygen Isotopic Compositions in Superscale Nitrate Mineral Deposits from Turpan-Hami Basin,Xinjiang,China and Its Significance显示文摘在东方 Xinjiang 的 Turpan-Hami 盆是在地球上的最干燥的区域和首要的环境之一形成并且保存硝酸盐。在这盆的大硝酸盐矿石域最近被发现。它在那里被估计是硝酸盐的大约 25 亿个大量资源,并且数量像在智利的 Atacama 荒芜的超级规模的硝酸盐沉积物一样。硝酸盐是有团无关的分别(MIF ) 的一些矿物质之一,并且氧同位素 MIF 是一个有效方法决定硝酸盐的来源。17O,硝酸盐的 18O 被氟化作用和热分解方法测量。日期显示这是氧同位素 MIF 位于内陆硝酸盐矿物质的第一次。结果由二个方法获得了是类似的, 17O=17O0.52 映杯吗? | LI Yanhe QIN Yan LIU Feng HOU Kejun WAN Defang | 2010 | Acta Geologica Sinica(English Edition)2010，84，6： | 5 |
| 7 | N and O isotopes and the ore-forming mechanism of nitrate deposits in the Turpan-Hami Basin,Xinjiang,China显示文摘The recently discovered nitrate ore field in the Turpan-Hami Basin of western China represents an estimated resource of 2.5 billion tons, and is comparable in scale to the Atacama Desert super-scale nitrate deposit in Chile. The research on this area is rarely carried out, and the origin of the deposits remains uncertain. In this study, new methods were used to systematically analyze N and O isotopes in nitrate minerals collected from the Kumutage, Xiaocaohu, Wuzongbulak, Dawadi, Tuyugou, and Shaer ore deposits in the Turpan-Hami Basin. The data showed that the δ15NAir value ranges from 0.7‰ to 27.6‰, but mostly between 2‰ and 6‰, which was similar to atmospheric NO3-. The 18O was highly enriched with δ18OV-SMOW varying from 30.2‰ to 46.7‰. This differs from levels in deposits derived from microbial nitrogen fixation, but is similar to those in atmospheric nitrates. N and O isotopes data indicated that nitrate deposits in Turpan-Hami Basin must be the result of deposition of atmospheric nitrate particles. Although atmospheric nitrate particles are common, the nitrate deposits could form only under the condition of long-term extreme drought climate and very limited biological activity. This paper summarized the ore-forming mechanism of different types nitrate deposits based on their geological setting. | QIN Yan LI YanHe LIU Feng HOU KeJun WAN DeFang ZHANG Cheng | 2012 | Science China Earth Sciences2012，55，2： | 5 |
| 8 | Effect of methotrexate combined with Sanhuang Yilong decoction on serum and synovial fluid aquaporin levels in rheumatoid arthritis dampness-heat blockage syndrome显示文摘OBJECTIVE:To study the effect of methotrexate(MTX) combined with Sanhuang Yilong decoction(SYD) on aquaporin(AQP) expression,and to explore the role of AQPs in the pathogenesis of rheumatoid arthritis(RA).METHODS:A total of 118 dampness-heat blockage type RA patients who were hospitalized in the General Chengdu Military Hospital between January2014 and December 2016 were selected as subjects in this study(30 patient of these patients with knee joint effusion were assigned to the RA synovial fluid group).For the pre-treatment controlgroups,30 healthy volunteers were recruited as the healthy control group and 30 osteoarthritis(OA) patients with knee joint effusion were included as OA synovial fluid control group.The RA dampnessheat blockage syndrome treatment groups were divided into 45 cases in the combined group and 45 cases in the MTX group.The combined group received MTX combined with SYD treatment while the MTX group received MTX alone.AQP1,AQP2 and AQP3 expressions were detected in the serum and synovial fluid.RESULTS:AQP1 had the highest expression,followed by AQP3,and AQP2.The serum levels of AQP1,AQP2 and AQP3 were all significantly lower than those in the healthy volunteers(P < 0.05),while the synovial fluid AQP1,AQP2 and AQP3 expression in the RA group were comparable to these in the OA groups(P > 0.05).After treatment for2 weeks,serum AQP1,AQP2,AQP3 were significantly increased and erythrocyte sedimentation rate,C-reactive protein,disease activity score of 28 joints were decreased in the combined group(P < 0.05).CONCLUSION:Abnormal expression of AQPs inhibits water metabolism in RA dampness-heat blockage syndrome,so liquid is accumulated at the joint,which may play an important role in the pathogenesis of RA.MTX combined with SYD for the treatment of RA can effectively increase AQP expression. | Liu Defang Yan Jiao Guo Mingyang Luo Yong Yang Mei Yang Min Zou Longfu Li Wan Zhong Xiaolong Liu Tao Hu Yonghe | 2018 | Journal of Traditional Chinese Medicine2018，38，4： | 4 |
| 9 | Effect of Sanhuangyilong decoction plus methotrexate on tumor necrosis factor alpha and interferon gamma in serum and synovial fluid in rheumatoid arthritis patients with symptom pattern of damp heat obstruction显示文摘OBJECTIVE: To investigate the effect of Sanhuangyilong decoction plus methotrexate(MTX) on Interferon gamma(IFN-γ) and tumor necrosis factor alpha(TNF-α) in the serum and synovial fluid of rheumatoid arthritis(RA) patients with damp-heat-obstruction symptom pattern, Sanhuangyilong decoction and the role of TNF-α and IFN-γ in the development of RA.METHODS: RA inpatients with damp-heat-obstruction symptom pattern(partly with knee joint effusion) were selected as the research subjects. Before the treatment, healthy subjects and osteoarthritis(OA) patients with knee joint effusion were assigned to the serum control group and the synovial fluid control group, respectively; during the treatment, RA patients with damp-heat-obstruction symptom pattern were divided into two groups:one is combined group that was administered Sanhuangyilong decoction plus MTX; the other group was MTX group that received MTX only. The expression levels of TNF-α and IFN-γ in the serum and synovial fluid were measured with enzyme-linked immunosorbent assay(ELISA) before and after the treatment, and the peripheral blood levels of erythrocyte sedimentation rate(ESR), C-reactive protein(CRP) and disease activity score in 28 joints(DAS28)were determined.RESULTS: Before treatment, the serum levels of TNF-α and IFN-γ in the RA patients with dampheat-obstruction symptom pattern were higher than those in healthy control group(P < 0.05).The expression levels of TNF-α and IFN-γ in the synovial fluid of the RA patients were higher than those in the serum of the RA patients(P < 0.05). The expression levels of TNF-α and IFN-γ in the synovial fluid of the RA patients were higher than those of the sy-novial fluid of the osteoarthritis patients(P < 0.05).The expression of TNF-α and IFN-γ in the serum and synovial fluid of the RA patients had no correlation with the inflammatory activity index ESR, CRP,or DAS28(P > 0.05). After 2 weeks of treatment, the expression level of TNF-α and IFN-γ in the combined group had increased, although the difference was not statistically significant(P > 0.05); in contrast, ESR, CRP, and DAS28 decreased, and the difference was statistically significant(P < 0.01). After 4 weeks of therapy, TNF-alpha and IFN-γ, ESR,CRP, and DAS28 in the combined group decreased compared with the before-treatment levels(P <0.01). After 2 w of treatment, the differences in the TNF-α and IFN-γ expression levels in the combined group were not statistically significant(P > 0.05)compared with that in the MTX group, although there were statistically significant differences in the ESR, CRP, and DAS28(P < 0.05). After 4 weeks of treatment, differences in TNF-α, IFN-γ, ESR, CRP, and DAS28 in the combined group compared with MTX group were statistically significant(P < 0.01).CONCLUSION: TNF-α and IFN-γ might be involved in the development of RA. The RA patients with damp-heat-obstruction symptom pattern show better benefits from the treatment of Sanhuangyilong decoction plus MTX, and the treatment is superior to that of using MTX only. | Liu Defang Yan Jiao Yun Mingdong Yang Ming Luo Yong Zhang Jun Guo Mingyang Yang Mei Yuan Weili Zou Wei Li Hua Hu Yonghe | 2016 | Journal of Traditional Chinese Medicine2016，36，5： | 4 |
| 10 | Structure and superconductivity of hydrides at high pressures显示文摘Hydrogen atoms can provide high phonon frequencies and strong electron–phonon coupling in hydrogen-rich materials,which are believed to be potential high-temperature superconductors at lower pressure than metallic hydrogen.Especially,recently both of theoretical and experimental reports on sulfur hydrides under pressure exhibiting superconductivity at temperatures as high as 200 K have further stimulated an intense search for room-temperature superconductors in hydrides.his review focuses on crystal structures,stabilities,pressure-induced transformations,metallization,and superconductivity of hydrogen-rich materials at high pressures. | Defang Duan Yunxian Liu Yanbin Ma Ziji Shao Bingbing Liu Tian Cui | 2017 | National Science Review2017，4，1： | 3 |
| 11 | Development and Evaluation of a Loop-mediated Isothermal Amplification(LAMP) Assay for Rapid Detection of Chinese Giant Salamander Ranavirus显示文摘Loop-mediated isothermal amplification(LAMP) is a novel nucleic acid diagnostic method that can amplify rapidly a target template under isothermal conditions. In this study, a LAMP assay for rapid detection of Chinese giant salamander ranavirus(CGSRV) was developed from culture isolates and clinical samples. The LAMP assay was developed by designing one set of four specific primers, targeting the major capsid protein(MCP) gene of CGSRV. Reaction time and temperature were optimal for 40 min at 62°C. The developed LAMP assay is specific and highly sensitive for CGSRV detection, the detection limit could reach about 5 copies of cloned viral genomic fragments. The sensitivity of the LAMP assay was about 1000 and 10-fold higher than that of both conventional and nested PCR, respectively. The LAMP amplification produces a typical ladder-like pattern of products on an agarose gel that can be visually inspected after addition of ethidium bromide. The LAMP assay was evaluated further with clinical samples, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for the detection of CGSRV. | Yi GENG Xingxing LIU Yan ZHOU Kaiyu WANG Xi PENG Zhijun ZHONG Xiaoli HUANG Defang CHEN | 2015 | Asian Herpetological Research2015，6，1： | 3 |
| 12 | Deep learning for in vitro prediction of pharmaceutical formulations显示文摘Current pharmaceutical formulation development still strongly relies on the traditional trialand-error methods of pharmaceutical scientists. This approach is laborious, time-consuming and costly.Recently, deep learning has been widely applied in many challenging domains because of its important capability of automatic feature extraction. The aim of the present research is to apply deep learning methods to predict pharmaceutical formulations. In this paper, two types of dosage forms were chosen as model systems. Evaluation criteria suitable for pharmaceutics were applied to assess the performance of the models. Moreover, an automatic dataset selection algorithm was developed for selecting the representative data as validation and test datasets. Six machine learning methods were compared with deep learning. Results showed that the accuracies of both two deep neural networks were above 80% and higher than other machine learning models; the latter showed good prediction of pharmaceutical formulations. In summary, deep learning employing an automatic data splitting algorithm and the evaluation criteria suitable for pharmaceutical formulation data was developed for the prediction of pharmaceutical formulations for the first time. The cross-disciplinary integration of pharmaceutics and artificial intelligence may shift the paradigm of pharmaceutical research from experience-dependent studies to data-driven methodologies. | Yilong Yang Zhuyifan Ye Yan Su Qianqian Zhao Xiaoshan Li Defang Ouyang | 2019 | Acta Pharmaceutica Sinica B2019，9，1： | 3 |
| 13 | Celastrol suppresses colorectal cancer via covalent targeting peroxiredoxin 1显示文摘As a terpenoids natural product isolated from the plant Thunder God Vine,Celastrol is widely studied for its pharmacological activities,including anti-tumor activities.The clinical application of Celastrol is strictly limited due to its severe side effects,whereas previously revealed targets and mechanism of Celastrol seldom reduce its in vivo toxicity via structural optimization.Target identification has a far-reaching influence on the development of innovative drugs,and omics data has been widely used for unbiased target prediction.However,it is difficult to enrich target of specific phenotype from thousands of genes or proteins,especially for natural products with broad promising activities.Here,we developed a text-mining-based web-server tool to enrich targets from omics data of inquired compounds.Then peroxiredoxin 1(PRDX1)was identified as the ROS-manipulating target protein of Celastrol in colorectal cancer.Our solved high-resolution crystal structure revealed the unique covalent binding mode of Celastrol with PRDX1.New derivative compound 19-048 with improved potency against PRDX1 and selectivity towards PRDX2~PRDX6 were synthesized based on crystal structure analysis.Both Celastrol and 19-048 effectively suppressed the proliferation of colorectal cancer cells.The anti-tumor efficacy of Celastrol and 19-048 was significantly diminished on xenograft nude mice bearing PRDX1 knock-down colorectal cancer cells.Several downstream genes of p53 signaling pathway were dramatically up-regulated with Celastrol or 19-048 treatment.Our findings reveal that the side effects of Celastrol could be reduced via structural modification,and PRDX1 inhibition is promising for the treatment of colorectal cancer. | Heng Xu Hongfang Zhao Chunyong Ding Defang Jiang Zijie Zhao Yang Li Xiaoyu Ding Jing Gao Hu Zhou Cheng Luo Guoqiang Chen Ao Zhang Ying Xu Hao Zhang | 2023 | Signal Transduction and Targeted Therapy2023，8，3： | 3 |
| 14 | Preparation and characterization of pelletized solid dispersion of resveratrol with mesoporous silica microparticles to improve dissolution by fluid-bed coating techniques显示文摘With hydrophilic surface and high surface area, porous silica has been applied to load insoluble drugs. Compared to solvent equilibrium method, resveratrol(RES)–mesoporous silica microparticles(MSM) solid dispersion prepared by fluid bed demonstrated higher drug loading and more complete dissolution. Pore volume and diameter have more remarkable effects than surface area to the drug loading and in vitro dissolution profiles. RES–polyethylene glycol solid dispersion with high drug loading showed fast but incomplete dissolution due to the recrystallization. The combination of fluid bed and MSM was an effective strategy to improve drug loading as well as dissolution for poorly water-soluble drugs. | Jian Li Xiaoqing Miao Tongkai Chen Defang Ouyang Ying Zheng | 2016 | Asian Journal of Pharmaceutical Sciences2016，11，4： | 3 |
| 15 | Archean Mass-independent Fractionation of Sulfur Isotope:New Evidence of Bedded Sulfide Deposits in the Yanlingguan-Shihezhuang area of Xintai,Shandong Province显示文摘摘要:太古代的 bedded 硫化物存款的多重硫同位素比率(34S/33S/32S ) 在 Xintai 在泰山组的 Yanlingguan 形成被测量,山东省,中国的东方;33S =?0.7% ~ 3.8% , 34S =0.1%-8.8% , 33S =?2.3% 到 ?0.7% 。硫同位素作文显示出明显的团无关的分别(MIF ) 签名。在太古代的硫化物的硫同位素的 MIF 的存在显示硫从太阳的紫外放射导致的暴烈的 SO2 的光化学的反应的产品,暗示臭氧盾没在在那时间的空气被形成,并且氧水平是不到 10 ? 5 PAL (现在的空气水平)。SO2 的光分解作用与否定 33S 生产的硫酸盐在暴烈的活动中心附近猛抛;并且有积极 33S 的元素 S 的产品远猛抛离开暴烈的活动中心。硫化物的更低的 33S 价值(-2.30% 到 ?0.25%) Shihezhuang 在暴烈的中心附近,并且硫主要从硫酸盐的表演由光分解作用生产了。更高的 33S 价值(-0.5% 到 ?2%) 显示 Yanlingguan 是远的离开暴烈的中心并且一些硫从硫酸盐,从元素 S 的另外一个由光分解作用生产了。从 Yanlingguan 的硫同位素的数据点在 34S-33S 的阴谋上在到 MFL (集体依赖分别线) 的线平行,证明暴烈的硫种类通过了进海洋的大气的周期,然后集体依赖分别发生在硫化物的免职期间。从 Shihezhuang 的硫同位素的数据点代表不同的硫来源的混合。 | LI Yanhe HOU Kejun WAN Defang YUE Guoliang | 2008 | Acta Geologica Sinica(English Edition)2008，82，2： | 2 |
| 16 | High-temperature superconductivity in sulfur hydride evidenced by alternating-current magnetic susceptibility显示文摘The search for high-temperature superconductivity is one of the research frontiers in physics. In the sulfur hydride system, an extremely high T_c(~200 K) has been recently developed at pressure. However, the Meissner effect measurement above megabar pressures is still a great challenge. Here, we report the superconductivity identification of sulfur hydride at pressure, employing an in situ alternating-current magnetic susceptibility technique. We determine the superconducting phase diagram, finding that superconductivity suddenly appears at 117 GPa and Tcreaches 183 K at 149 GPa before decreasing monotonically with increasing pressure. By means of theoretical calculations, we elucidate the variation of T_c in the low-pressure region in terms of the changing stoichiometry of sulfur hydride and the further decrease in Tcowing to a drop in the electron–phonon interaction parameter λ. This work provides a new insight into clarifying superconducting phenomena and anchoring the superconducting phase diagram in the hydrides. | Xiaoli Huang Xin Wang Defang Duan Bertil Sundqvist Xin Li Yanping Huang Hongyu Yu Fangfei Li Qiang Zhou Bingbing Liu Tian Cui | 2019 | National Science Review2019，6，4： | 2 |
| 17 | Dietary isoleucine improved flesh quality,muscle antioxidant capacity,and muscle growth associated with AKT/TOR/S6K1 and AKT/FOXO3a signaling in hybrid bagrid catfish(Pelteobagrus vachelli♀×Leiocassis longirostris♂)显示文摘Background:Muscle is the complex and heterogeneous tissue,which comprises the primary edible part of the trunk of fish and mammals.Previous studies have shown that dietary isoleucine(Ile)exerts beneficial effects on growth in aquatic animals.However,there were limited studies regarding the benefits of Ile on fish muscle and their effects on flesh quality and muscle growth.Thus,this study was conducted to explore whether dietary Ile had affected flesh quality and muscle growth in hybrid bagrid catfish(Pelteobagrus vachelli♀×Leiocassis longirostris♂).Methods:A total of 630 hybrid fish,with an initial average body weight of 33.11±0.09 g,were randomly allotted into seven experimental groups with three replicates each,and respectively fed seven diets with 5.0,7.5,10.0,12.5,15.0,17.5,and 20.0 g Ile/kg diets for 8 weeks.Results:In the present study,we demonstrated that Ile significantly:(1)increased muscle protein and lipid contents and the frequency distribution of myofibers with≤20μm and≥50μm of diameter;(2)improved pH value,shear force,cathepsin B and L activities,hydroxyproline content,resilience,cohesiveness,and decreased cooking loss,lactate content,hardness,springiness,gumminess,and chewiness;(3)decreased reactive oxygen species(ROS),malondialdehyde(MDA),and protein carbonyl(PC)contents,GCLC and Keap1 mRNA levels,and up-regulated CuZnSOD,CAT,GPX1a,GST,and Nrf2 mRNA levels;(4)up-regulated the insulin-like growth factor 1,2(IGF-1,IGF-2),insulin-like growth factor 1 receptor(IGF-1R),proliferating cell nuclear antigen(PCNA),Myf5,Myod,Myog,Mrf4,and MyHC mRNA levels,and decreased MSTN mRNAlevel;(5)increased muscle protein deposition by activating AKT-TOR-S6K1 and AKT-FOXO3a signaling pathways.Conclusion:These results revealed that dietary Ile improved flesh quality,which might be due to increasing nutritional content,physicochemical,texture parameters,and antioxidant ability;promoting muscle growth by affecting myocytes hyperplasia and hypertrophy,and muscle protein deposition associated with protein synthesis and degradation signaling pathways.Finally,the quadratic regression analysis of chewiness,ROS,and protein contents against dietary Ile levels suggested that the optimal dietary Ile levels for hybrid bagrid catfish was estimated to be 14.19,12.36,and 12.78 g/kg diet,corresponding to 36.59,31.87,and 32.96 g/kg dietary protein,respectively. | Qin Jiang Mingyao Yan Ye Zhao Xiaoqiu Zhou Long Yin Lin Feng Yang Liu Weidan Jiang Pei Wu Yan Wang Defang Chen Shiyong Yang Xiaoli Huang Jun Jiang | 2021 | Journal of Animal Science and Biotechnology2021，12，4： | 2 |
| 18 | Investigation of molecular aggregation mechanism of glipizide/cyclodextrin complexation by combined experimental and molecular modeling approaches显示文摘Cyclodextrin complexation is a wise strategy to enhance aqueous solubility of waterinsoluble drugs.However,the aggregation mechanism of drug-cyclodextrin complexes is still unclear.This research aimed to investigate the molecular aggregation mechanism of glipizide/cyclodextrin complexation by the combination of experimental and modeling methods.Binding free energies between glipizide and cyclodextrins from modeling calculations were higher than those by the phase solubility diagram method.Both experimental and modeling results showed that methylated-β-cyclodextrin exhibited the best solubilizing capability to glipizide.Size-measurement results confirmed the aggregation between glipizide and all four cyclodextrins in high concentrations.Glipizide/γ-cyclodextrin and glipizide/β-cyclodextrin complexes showed stronger aggregation trend than HP-β-cyclodextrin and methylated-β-cyclodextrin.The substituted groups in the rim of HP-β-cyclodextrin and methylated-β-cyclodextrin lead to weak aggregation.This research provided us a clear molecular mechanism of glipizide/cyclodextrin complexation and aggregation.This research will also benefit the formulation development of cyclodextrin solubilization. | Tianhe Huang Qianqian Zhao Yan Su Defang Ouyang | 2019 | Asian Journal of Pharmaceutical Sciences2019，14，6： | 2 |
| 19 | Application of a chitosan flocculant to water treatment显示文摘 | Defang Zeng Juanjuan Wu John F. Kennedy | 2007 | Carbohydrate Polymers2007，，1： | 2 |
| 20 | Pathological Changes in Andrias davidianus Infected with Chinese Giant Salamander Ranavirus显示文摘Chinese giant salamander ranavirus(CGSRV) is an emerging pathogen in captive populations of the Chinese giant salamander(Andrias davidianus). We processed 140 morbid Chinese giant salamanders from seven captive breeding populations over five years, and describe the disease associated with CGSRV infection. The most common gross signs were significant swelling of the legs and coelomic cavity, erythema of the legs and ventrum in juveniles; cutaneous erosions and ulcerations in adults, particularly the limbs and the head; and marked petechial or ecchymotic hemorrhages of the internal organs, particularly the liver, spleen and kidney. Histological examination showed degeneration, necrosis, and inflammation in many organs, particularly in the organs where hemorrhage was observed. There was evidence of eosinophilic inclusion bodies in degenerated and necrotic cells. We identified virus particles and empty capsids without viral nucleoid in the inclusion bodies using electron microscopy. Virus particles were hexagonal or round shape, and appeared in paracrystalline arrays, aggregates, or singly. All enveloped viral particles were 140–160 nm. Polymerase chain reaction followed by sequencing verified that the virus particles were CGSRV. These results collectively support that CGSRV was the etiologic agent responsible for these mass die-offs of the Chinese giant salamander. The pathology described herein will be useful in diagnosing cases of ranaviral disease caused by CGSRV, and provide evidence that this pathogen is a significant threat to the Chinese giant salamander. | Yi GENG Matthew James GRAY Kaiyu WANG Defang CHEN Ping OUYANG Xiaoli HUANG Changliang HE Zhijun ZHONG Zexiao YANG | 2016 | Asian Herpetological Research2016，7，4： | 2 |
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