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| 1 | CD13/APN expression in peripheral blood lymphocytes and skin lesions in patients with advanced psoriasis vulgaris显示文摘Objective: To observe the expression of CD13/APN in peripheral blood lymphocytes and skin lesions of patients with advanced psoriasis vulgaris, and discuss its effect on the pathogenesis of psoriasis. Methods: CD13 expression in peripheral blood lymphocytes and skin lesions was detected by flow cytometry and immunohistochemical technique, respectively. Results were compared with those of healthy controls. Results: CD13 expression was significantly higher in peripheral blood lymphocytes of patients with advanced psoriasis vulgaris than in that of healthy controls, and in skin lesions than in healthy skin tissues. The expression was mainly in the suprabasal layers of skin lesions, and positively correlated to PASI (R=0.78029). Conclusion: The significantly higher expression of CD13 in peripheral blood lymphocytes and skin lesions of the patients with advanced psoriasis vulgaris probably is related to immunological abnormality, blood vessel abnormality and proliferation of keratinocyte in the pathogenic course of psoriasis. It may be a novel and effective way to treat psoriasis with specific CD13 inhibitors. | Liu Taihua Liu Defang Chen Yihua Hu Zonghai Chen Lu Luo Chen Xu Zhejuan | 2009 | Journal of Medical Colleges of PLA(China)2009，24，1： | 5 |
| 2 | Development and Evaluation of a Loop-mediated Isothermal Amplification(LAMP) Assay for Rapid Detection of Chinese Giant Salamander Ranavirus显示文摘Loop-mediated isothermal amplification(LAMP) is a novel nucleic acid diagnostic method that can amplify rapidly a target template under isothermal conditions. In this study, a LAMP assay for rapid detection of Chinese giant salamander ranavirus(CGSRV) was developed from culture isolates and clinical samples. The LAMP assay was developed by designing one set of four specific primers, targeting the major capsid protein(MCP) gene of CGSRV. Reaction time and temperature were optimal for 40 min at 62°C. The developed LAMP assay is specific and highly sensitive for CGSRV detection, the detection limit could reach about 5 copies of cloned viral genomic fragments. The sensitivity of the LAMP assay was about 1000 and 10-fold higher than that of both conventional and nested PCR, respectively. The LAMP amplification produces a typical ladder-like pattern of products on an agarose gel that can be visually inspected after addition of ethidium bromide. The LAMP assay was evaluated further with clinical samples, and the results indicated the suitability and simplicity of the test as a rapid diagnostic tool for the detection of CGSRV. | Yi GENG Xingxing LIU Yan ZHOU Kaiyu WANG Xi PENG Zhijun ZHONG Xiaoli HUANG Defang CHEN | 2015 | Asian Herpetological Research2015，6，1： | 3 |
| 3 | Celastrol suppresses colorectal cancer via covalent targeting peroxiredoxin 1显示文摘As a terpenoids natural product isolated from the plant Thunder God Vine,Celastrol is widely studied for its pharmacological activities,including anti-tumor activities.The clinical application of Celastrol is strictly limited due to its severe side effects,whereas previously revealed targets and mechanism of Celastrol seldom reduce its in vivo toxicity via structural optimization.Target identification has a far-reaching influence on the development of innovative drugs,and omics data has been widely used for unbiased target prediction.However,it is difficult to enrich target of specific phenotype from thousands of genes or proteins,especially for natural products with broad promising activities.Here,we developed a text-mining-based web-server tool to enrich targets from omics data of inquired compounds.Then peroxiredoxin 1(PRDX1)was identified as the ROS-manipulating target protein of Celastrol in colorectal cancer.Our solved high-resolution crystal structure revealed the unique covalent binding mode of Celastrol with PRDX1.New derivative compound 19-048 with improved potency against PRDX1 and selectivity towards PRDX2~PRDX6 were synthesized based on crystal structure analysis.Both Celastrol and 19-048 effectively suppressed the proliferation of colorectal cancer cells.The anti-tumor efficacy of Celastrol and 19-048 was significantly diminished on xenograft nude mice bearing PRDX1 knock-down colorectal cancer cells.Several downstream genes of p53 signaling pathway were dramatically up-regulated with Celastrol or 19-048 treatment.Our findings reveal that the side effects of Celastrol could be reduced via structural modification,and PRDX1 inhibition is promising for the treatment of colorectal cancer. | Heng Xu Hongfang Zhao Chunyong Ding Defang Jiang Zijie Zhao Yang Li Xiaoyu Ding Jing Gao Hu Zhou Cheng Luo Guoqiang Chen Ao Zhang Ying Xu Hao Zhang | 2023 | Signal Transduction and Targeted Therapy2023，8，3： | 3 |
| 4 | Preparation and characterization of pelletized solid dispersion of resveratrol with mesoporous silica microparticles to improve dissolution by fluid-bed coating techniques显示文摘With hydrophilic surface and high surface area, porous silica has been applied to load insoluble drugs. Compared to solvent equilibrium method, resveratrol(RES)–mesoporous silica microparticles(MSM) solid dispersion prepared by fluid bed demonstrated higher drug loading and more complete dissolution. Pore volume and diameter have more remarkable effects than surface area to the drug loading and in vitro dissolution profiles. RES–polyethylene glycol solid dispersion with high drug loading showed fast but incomplete dissolution due to the recrystallization. The combination of fluid bed and MSM was an effective strategy to improve drug loading as well as dissolution for poorly water-soluble drugs. | Jian Li Xiaoqing Miao Tongkai Chen Defang Ouyang Ying Zheng | 2016 | Asian Journal of Pharmaceutical Sciences2016，11，4： | 3 |
| 5 | Dietary isoleucine improved flesh quality,muscle antioxidant capacity,and muscle growth associated with AKT/TOR/S6K1 and AKT/FOXO3a signaling in hybrid bagrid catfish(Pelteobagrus vachelli♀×Leiocassis longirostris♂)显示文摘Background:Muscle is the complex and heterogeneous tissue,which comprises the primary edible part of the trunk of fish and mammals.Previous studies have shown that dietary isoleucine(Ile)exerts beneficial effects on growth in aquatic animals.However,there were limited studies regarding the benefits of Ile on fish muscle and their effects on flesh quality and muscle growth.Thus,this study was conducted to explore whether dietary Ile had affected flesh quality and muscle growth in hybrid bagrid catfish(Pelteobagrus vachelli♀×Leiocassis longirostris♂).Methods:A total of 630 hybrid fish,with an initial average body weight of 33.11±0.09 g,were randomly allotted into seven experimental groups with three replicates each,and respectively fed seven diets with 5.0,7.5,10.0,12.5,15.0,17.5,and 20.0 g Ile/kg diets for 8 weeks.Results:In the present study,we demonstrated that Ile significantly:(1)increased muscle protein and lipid contents and the frequency distribution of myofibers with≤20μm and≥50μm of diameter;(2)improved pH value,shear force,cathepsin B and L activities,hydroxyproline content,resilience,cohesiveness,and decreased cooking loss,lactate content,hardness,springiness,gumminess,and chewiness;(3)decreased reactive oxygen species(ROS),malondialdehyde(MDA),and protein carbonyl(PC)contents,GCLC and Keap1 mRNA levels,and up-regulated CuZnSOD,CAT,GPX1a,GST,and Nrf2 mRNA levels;(4)up-regulated the insulin-like growth factor 1,2(IGF-1,IGF-2),insulin-like growth factor 1 receptor(IGF-1R),proliferating cell nuclear antigen(PCNA),Myf5,Myod,Myog,Mrf4,and MyHC mRNA levels,and decreased MSTN mRNAlevel;(5)increased muscle protein deposition by activating AKT-TOR-S6K1 and AKT-FOXO3a signaling pathways.Conclusion:These results revealed that dietary Ile improved flesh quality,which might be due to increasing nutritional content,physicochemical,texture parameters,and antioxidant ability;promoting muscle growth by affecting myocytes hyperplasia and hypertrophy,and muscle protein deposition associated with protein synthesis and degradation signaling pathways.Finally,the quadratic regression analysis of chewiness,ROS,and protein contents against dietary Ile levels suggested that the optimal dietary Ile levels for hybrid bagrid catfish was estimated to be 14.19,12.36,and 12.78 g/kg diet,corresponding to 36.59,31.87,and 32.96 g/kg dietary protein,respectively. | Qin Jiang Mingyao Yan Ye Zhao Xiaoqiu Zhou Long Yin Lin Feng Yang Liu Weidan Jiang Pei Wu Yan Wang Defang Chen Shiyong Yang Xiaoli Huang Jun Jiang | 2021 | Journal of Animal Science and Biotechnology2021，12，4： | 2 |
| 6 | Pathological Changes in Andrias davidianus Infected with Chinese Giant Salamander Ranavirus显示文摘Chinese giant salamander ranavirus(CGSRV) is an emerging pathogen in captive populations of the Chinese giant salamander(Andrias davidianus). We processed 140 morbid Chinese giant salamanders from seven captive breeding populations over five years, and describe the disease associated with CGSRV infection. The most common gross signs were significant swelling of the legs and coelomic cavity, erythema of the legs and ventrum in juveniles; cutaneous erosions and ulcerations in adults, particularly the limbs and the head; and marked petechial or ecchymotic hemorrhages of the internal organs, particularly the liver, spleen and kidney. Histological examination showed degeneration, necrosis, and inflammation in many organs, particularly in the organs where hemorrhage was observed. There was evidence of eosinophilic inclusion bodies in degenerated and necrotic cells. We identified virus particles and empty capsids without viral nucleoid in the inclusion bodies using electron microscopy. Virus particles were hexagonal or round shape, and appeared in paracrystalline arrays, aggregates, or singly. All enveloped viral particles were 140–160 nm. Polymerase chain reaction followed by sequencing verified that the virus particles were CGSRV. These results collectively support that CGSRV was the etiologic agent responsible for these mass die-offs of the Chinese giant salamander. The pathology described herein will be useful in diagnosing cases of ranaviral disease caused by CGSRV, and provide evidence that this pathogen is a significant threat to the Chinese giant salamander. | Yi GENG Matthew James GRAY Kaiyu WANG Defang CHEN Ping OUYANG Xiaoli HUANG Changliang HE Zhijun ZHONG Zexiao YANG | 2016 | Asian Herpetological Research2016，7，4： | 2 |
| 7 | lsoliquiritigenin induces monocytic differentiation of HL-60 cells显示文摘 | LI Defang WANG Zhenhua CHEN Hongmei | | 0，，06： | 1 |
| 8 | Direct discriminant locality preserving projection with Hammerstein polynomial expansion显示文摘 | Chen Xi Zhang Jiashu Li Defang | 2012 | IEEE Transactions on Image Processing2012，21，12： | 1 |
| 9 | Genome analysis and development of infectious cDNA clone of a virulence-attenuated strain of foot-and-mouth disease virus type Asia 1 from China显示文摘 | Aiguo Xin Huachun Li Le Li Defang Liao Yongqin Yang Nianzu Zhang Baoshan Chen | 2009 | Veterinary Microbiology2009，，3： | 1 |
| 10 | The correlation of stoichiometry between boron-rich LaB6 targets and LaB6 films 显示文摘 | Yan Wu Guanghui Minn Defang Chen | 2015 | ScienceDirect2015，41，： | 1 |
| 11 | Iterative gradient projection algorithm for two-dimensional compressed sensing sparse image reconstruction显示文摘 | Gao Chen Defang Li Jiashu Zhang | 2014 | Signal Processing2014，104，： | 1 |
| 12 | Similarity reductions of the KP equation by a direct method显示文摘 | Lou Senyue Ruan Hangyu Chen Defang | 1991 | J Phys A1991，24，： | 1 |
| 13 | Isoliquiritigenin induces monocytic differentiation of HL-60 cells显示文摘 | Defang Li Zhenhua Wang Hongmei Chen Jingying Wang Qiusheng Zheng Jing Shang Ji Li | 2008 | Free Radical Biology and Medicine2008，，6： | 1 |
| 14 | Artificial intelligence-aided discovery of prolyl hydroxylase 2 inhibitors to stabilize hypoxia inducible factor-1α and promote angiogenesis显示文摘From ZINC database with a total of 1.8 million small molecules, four compounds are identified as prolyl hydroxylase 2 inhibitors through a virtual screening workflow that sequentially incorporates machine learning, molecular docking, and molecular dynamics. Among them, compound 103,(E)-5-(5-((2-(1Htetrazol-5-yl)hydrazineylidene)methyl)furan-2-yl)isoindoline-1,3-dione, promotes the migration and capillary tube formation capacity of human umbilical vein endothelial cells through enhancing the stability of hypoxia inducible factor-1α and increasing the level of vascular endothelial growth factor. | Jianzhong Zhu Cheng Chen Jie Dong Shasha Cheng Guodong Li Chunming Wang Defang Ouyang Chung-Hang Leung Ligen Lin | 2023 | Chinese Chemical Letters2023，34，2： | 0 |
| 15 | Stability of diatomaceous silica and its geochemical implication显示文摘An experiment of stability of diatomaceous silica was performed. The results show that pH value and temperature dominantly influence the stability. The pH value concerning largely dissolving diatomaceous silica is 2-3 higher than that amorphous silica due to the buffer action of coexistent clay minerals. The solubility of diatomaceous silica rises distinctly when temperature is over 40℃, which suggests the cause of distribution of large-scale diatomite de- | Huanxin Weng Zhongyue Shen Mingyong Yuan Defang Guo Jianyu Chen | 1999 | Chinese Science Bulletin1999，44，23： | 0 |
| 16 | Progress in oncolytic viruses modified with nanomaterials for intravenous application显示文摘In oncolytic virus(OV)therapy,a critical component of tumor immunotherapy,viruses selectively infect,replicate within,and eventually destroy tumor cells.Simultaneously,this therapy activates immune responses and mobilizes immune cells,thereby eliminating residual or distant cancer cells.However,because of OVs’high immunogenicity and immune clearance during circulation,their clinical applications are currently limited to intratumoral injections,and their use is severely restricted.In recent years,numerous studies have used nanomaterials to modify OVs to decrease virulence and increase safety for intravenous injection.The most commonly used nanomaterials for modifying OVs are liposomes,polymers,and albumin,because of their biosafety,practicability,and effectiveness.The aim of this review is to summarize progress in the use of these nanomaterials in preclinical experiments to modify OVs and to discuss the challenges encountered from basic research to clinical application. | Liting Chen Zhijun Ma Chen Xu Youbang Xie Defang Ouyang Shuhui Song Xiao Zhao Funan Liu | 2023 | Cancer Biology & Medicine2023，20，11： | 0 |
| 17 | New Cage-Like Cerium Trihydride Stabilized at Ambient Conditions显示文摘Metal hydrides,generally formed by high pressure combined with high-temperature conditions,have attracted substantial interest due to their promising high-energy density and high-temperature superconductivity.Although the synthesis and properties of these hydrides have been extensively studied,an important challenge is the lack of an efficient method to retain high-pressure phases.Herein,we have successfully quenched a high-pressure phase to ambient conditions with the precise control of different compression pathways.A newβ-CeH_(3) phase(space group Pm3n)isostructural toβ-UH_(3) phase was synthesized by the reaction of Ce and H2 above 33.0 GPa in a laser-heated diamond anvil cell.Theβ-CeH_(3)phase has a cage-like framework structure that can be retained at ambient conditions.The electrical resistance as a function of temperature determines its metallic properties,but no superconductivity is observed in the current temperature range.Thermodynamic and dynamic calculations further prove the stability ofβ-CeH_(3) at ambient pressure.This work demonstrates that different synthesized paths will drive the formation of different products. | Xin Li Xiaoli Huang Wuhao Chen Di Zhou Hui Xie Quan Zhuang Defang Duan Tian Cui | 2022 | CCS Chemistry2022，4，3： | 0 |
| 18 | Glycerol steam reforming over hydrothermal synthetic Ni-Ca/attapulgite for green hydrogen generation显示文摘Glycerol steam reforming(GSR)is one of the promising technologies that can realize renewable hydrogen production and efficient utilization of crude glycerol.To illuminate the functions of Ca content(3%,6%,9%,and 12%,by mass)and preparation method for Ni/ATP catalyst structure and its catalytic behaviors,the Ni-xCa/ATP(x=3%,6%,9%,and 12%,by mass)catalysts are prepared by co-impregnation(ci)and hydrothermal synthesis(hs)method and then tested in GSR.Characterization results of XRD,N_(2) adsorption–desorption,H_(2)-TPR,HRTEM,XPS,and NH_(3)/CO_(2)-TPD demonstrate that the combined effect between appropriate Ca additive(6%,by mass)and hs enhance catalyst reducibility,uniform distribution of Ca additive and nickel species over ATP,and adsorption for CO_(2).This attributes to hs method protects the ATP framework through suppressing the interaction of Ca with ATP and promotes the formation of NiCaOx interface sites.Therefore,Ni-6Ca/ATP-hs exhibits the highest conversion(86.77%)of glycerol to gas product and H_(2) yield(76.17%)and selectivity(58.56%)during GSR.Furthermore,XRD,HRTEM,TGDTG and Raman analyses confirm that Ni-6Ca/ATP-hs also reveals outstanding anti-sintering and coke resistance.In addition,the structural evolution process of Ni/ATP catalyst with Ca introduction and hs method is presented.Considering the high performance,simple preparation process and low cost,the as-prepared catalyst providing new opportunities for utilization of glycerol derived from biodiesel industry. | Yishuang Wang Na Li Mingqiang Chen Defang Liang Chang Li Quan Liu Zhonglian Yang Jun Wang | 2022 | Chinese Journal of Chemical Engineering2022，35，8： | 0 |
| 19 | High T_(c) superconductivity in layered hydrides XH_(15) (X = Ca, Sr, Y, La) under high pressures显示文摘The theoretical predictions and experimental synthesis of H_(3)S and LaH_(10) superconductors with record high superconducting transition temperatures(T_(c))have promoted the hydrogen-based superconducors to be a research hotspot in the field of solid-state physics.Here,we predict an unprecedented layered structure CaH15,with high T_(c) of 189 K at 200 GPa using ab initio calculations.As concerns the novel structure,one layer is made of a hydrogen nonagon,the other layer consists of a Ca atom and six H_(2) molecular units surrounding the Ca atom.This layered structure was also found in SrH_(15),YH_(15),and LaH_(15) at high pressures,each materials exhibit high T_(c) especially YH_(15) can reach above 200 K at 220 GPa.It represents the second class of layered superhydrides with high value of Tc after pentagraphene like HfH10. | Yue Chen Zhengtao Liu Ziyue Lin Qiwen Jiang Mingyang Du Zihan Zhang Hao Song Hui Xie Tian Cui Defang Duan | 2022 | Frontiers of physics2022，17，6： | 0 |
| 20 | Analysis of the common model error on velocity field under Colored noise model by GPS and InSAR: A case study in the Nepal and everest region显示文摘The accuracy of the velocity field will be affected by the noise model and common mode errors through GPS time series analysis.In order to analyze the influence of these two factors on the accuracy of the velocity field,two kinds of data are used,including the three-year observation from 20 permanent GPS stations with high spatial correlation in the Everest,which is about 650 km from north to south and 1068 km from east to west,and three-year 80 ascending images and 141 descending images from sentinel-1A,which are processed by GAMIT/GLOBK software and Small Baseline Subset-Interferometric Synthetic Aperture Radar method(SBAS-InSAR),respectively.The vertical deformation rate is solved by time series analysis through a self-made adaptive algorithm.In the analysis,the linear change rate,period,half period coefficient,and residual sequence of all stations are solved by using James L.Davis periodic model.The noise type of residual sequence is analyzed by the power spectrum model.The spatio-temporal correlated noise,Common Mode Error(CME),is extracted by the Principal Component Analysis(PCA)and Karhunen-Loeve(KLE)methods.The results show that noises can be best described by“flicker noise+white noise”model.After the removal of CME,the R^(2) estimates of all stations are above 0.8,with RMS value of velocity field decreasing from 1.428 mm/yr to 1.062 mm/yr and 1.063 mm/yr to 0.815 mm/yr,in N and E directions,respectively,indicating that the influence of CME can't be ignored in the extraction of the high-precision velocity field in the Nepal and Everest region. | Wei Xu Gang Chen Kaihua Ding Defang Yang Yanfa Si Xiaoying Yang | 2022 | Geodesy and Geodynamics2022，13，4： | 0 |
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