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| 1 | c-Fos overexpression increases the proliferation of human hepatocytes by stabilizing nuclear Cyclin D1显示文摘AIM: To investigate the effect of stable c-Fos overexpression on immortalized human hepatocyte (IHH) proliferation. METHODS: IHHs stably transfected with c-Fos (IHH-Fos) or an empty vector (IHH-C) were grown in medium supplemented with 1% serum or stimulated with 10% serum. Cell proliferation was assessed by cell counts, 3H-thymidine uptake and fl ow cytometry analyses. The levels of cell cycle regulatory proteins (Cyclin D1, E, A) cyclin dependent kinases (cdk) cdk2, cdk4, cdk6, and their inhibitors p15, p16, p21, p27, to- tal and phosphorylated GSK-3β and epidermal growth factor receptor (EGF-R) were assayed by Western blot- ting. Analysis of Cyclin D1 mRNA levels was performed by reverse transcription-polymerase chain reaction and real-time polymerase chain reaction (PCR) analysis. Stability of Cyclin D1 was studied by cycloheximide blockade experiments. RESULTS: Stable c-Fos overexpression increased cell proliferation under low serum conditions and resulted in a two-fold increase in [3H]-thymidine incorpora- tion following serum addition. Cell cycle analysis by fl ow cytometry showed that c-Fos accelerated the cell cycle kinetics. Following serum stimulation, Cyclin D1 was more abundantly expressed in c-Fos overexpressing cells. Cyclin D1 accumulation did not result from increased transcriptional activation, but from nuclear stabilization. Overexpression of c-Fos correlated with higher nuclear levels of inactive phosphorylated GSK- 3β, a kinase involved in Cyclin D1 degradation and higher levels of EGF-R mRNA, and EGF-R protein compared to IHH-C both in serum starved, and in serum stimulated cells. Abrogation of EGF-R signalling in IHH- Fos by treatment with AG1478, a specif ic EGF-R tyrosine kinase inhibitor, prevented the phosphorylation of GSK-3β induced by serum stimulation and decreased Cyclin D1 stability in the nucleus. CONCLUSION: Our results clearly indicate a positive role for c-Fos in cell cycle regulation in hepatocytes. Importantly, we delineate a new mechanism by which c-Fos could contribute to hepatocarcinogenesis through stabilization of Cyclin D1 within the nucleus, evoking a new feature to c-Fos implication in hepatocellular carcinoma. | Meryem Güller Kahina Toualbi-Abed Agnès Legrand Laurence Michel Alain Mauviel Dominique Bernuau Fanny Daniel | 2008 | World Journal of Gastroenterology2008，14，41： | 6 |
| 2 | Mechanisms involved in the inhibition of myoblast proliferation and differentiation by myostatin显示文摘 | Dominique Joulia Henri Bernardi Véronique Garandel Fanjaniriana Rabenoelina Barbara Vernus Gérard Cabello | 2003 | Experimental Cell Research2003，，2： | 3 |
| 3 | Saccharomyces boulardii Does Not Prevent Relapse of Crohn’s Disease显示文摘 | Arnaud Bourreille Guillaume Cadiot Gérard Le Dreau David Laharie Laurent Beaugerie Jean–Louis Dupas Philippe Marteau Patrick Rampal Dominique Moyse Ashraf Saleh Marie–Emmanuelle Le Guern Jean–Paul Galmiche | 2013 | Clinical Gastroenterology and Hepatology2013，，8： | 3 |
| 4 | Oxidative stress promotes pathologic polyploidization in nonalcoholic fatty liver disease显示文摘 | Gentric Géraldine Maillet Vanessa Paradis Valérie Couton Dominique L’Hermitte Antoine Panasyuk Ganna Fromenty Bernard Celton-Morizur Séverine Desdouets Chantal | 2015 | Journal of Clinical Investigation2015，，： | 2 |
| 5 | The pharmacokinetics of 750 μg levonorgestrel following administration of one single dose or two doses at 12 - or 24 -h in terval 显示文摘 | DOMINIQUE T ERIN G ANDRE U | 2001 | Contraception2001，64，3： | 1 |
| 6 | More epidural than intravenous sufentanil is required to provide comparable postoperative pain relief显示文摘 | CHRISTOTOPHE M BRUNO G DOMINIQUE F | 2001 | Anesth analg2001，93，2： | 1 |
| 7 | Myocarditis in patients with clinical presentation of myocardial infarction and normal coronary angiograms显示文摘 | Laure Sarda Patrice Colin Franck Boccara Doumit Daou Rachida Lebtahi Marc Faraggi Charles Nguyen Ariel Cohen Michel S Slama Philippe G Steg Dominique Le Guludec | 2001 | Journal of the American College of Cardiology2001，，3： | 1 |
| 8 | Empirical estimation of tail dependence using Copulas application to Asian mar- kets 显示文摘 | CYRIL C DOMINIQUE G | 2005 | Quantitative Finance2005，，5： | 1 |
| 9 | The impact of public R&D expenditure on business R&D 显示文摘 | Dominique G Bruno P | 2003 | Economics of Innovation and New Technology2003，，12： | 1 |
| 10 | Prognosis value of radionu clide angiography in patients with right ventricular arrhythmias 显示文摘 | Dominique LG Helene G Raphael P | 2001 | Circ2001，103，15： | 1 |
| 11 | Mutational analysis of the catalytic centre of the Citrobacter freundii AmpD N - acetylmuramyl - L - alanine amidase 显示文摘 | Catherine G Dominique D Bart D | 2004 | Biochemical Society2004，377，11： | 1 |
| 12 | Measurement of procalcitonin levels in children with Bacterial or viral meningitis显示文摘 | Dominique G Josette R | 1997 | Clinical Infectious Diseases1997，24，： | 1 |
| 13 | Magnesium alloys laser(Nd:YA G)cladding and alloying with side injection of aluminium powder显示文摘 | Sorin I Pierre S Dominique G | 2004 | Applied Surface Science2004，225，： | 1 |
| 14 | Automated retrieval of forest structure variables based on multi-scale texture analysis of VHR satellite imagery显示文摘 | BENOIT B DOMINIQUE G | 2014 | ISPRS JournaI of photogrammetry and Remote Sensing2014，96，： | 1 |
| 15 | Empirical estimation of tail correlation using copulas:application to Asian markets显示文摘 | Cyril C Dominique M G | 2005 | Quantitative Finance2005，5，5： | 1 |
| 16 | A new material model for 2D numerical simulation of serrated chip formation when machining titanium alloy Ti-6Al-4V显示文摘 | MADALINA C DOMINIQUE C FRANCK G | | 0，，03： | 1 |
| 17 | Simple design rules for optimal design of dielectric temperature-compensated sapphire resonators 显示文摘 | Tobar M E Dominique Cros John G Hartnett | 2003 | IEEE trans on Ultrasonics Ferroelectricy Control2003，50，9： | 1 |
| 18 | Laparoscopic Liver Resection for Malignant Liver Tumors: Preliminary Results of a Multicenter European Study显示文摘 | Jean-Fran?ois Gigot David Glineur Juan Santiago Azagra Martine Goergen Marc Ceuterick Mario Morino José Etienne Jacques Marescaux Didier Mutter Ludo van Krunckelsven Bernard Descottes Dominique Valleix Fran?ois Lachachi Claude Bertrand Baudouin Mansvelt G | 2002 | Annals of Surgery2002，，1： | 1 |
| 19 | Marrow stromal cells for cellular cardiomyoplasty: feasibility and potential clinical advantages显示文摘 | DOMINIQUE S T JACQUES G | 2000 | J Thorac Cardiovasc Surg2000，120，5： | 1 |
| 20 | Drip flow variations under a stalactite of the Pere Noel Cave (Belgium); Evidence of seasonal variations and air pressure constraints显示文摘 | Dominique D G | 1998 | Journal of Hydrology1998，211，14： | 1 |
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