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| 1 | Embryoid bodies formation and differentiation from mouse embryonic stem cells in collagen/Matrigel scaffolds显示文摘Embryonic stem (ES) cells have the potential to develop into any type of tissue and are considered as a promising source of seeding cells for tissue engineering and transplantation therapy.The main catalyst for ES cells differentiation is the growth into embryoid bodies (EBs),which are utilized widely as the trigger of in vitro differentiation.In this study,a novel method for generating EBs from mouse ES cells through culture in collagen/Matrigel scaffolds was successfully established.When single ES cells were seeded in three dimensional collagen/Matrigel scaffolds,they grew into aggregates gradually and formed simple EBs with circular structures.After 7 days' culture,they formed into cystic EBs that would eventually differentiate into the three embryonic germ layers.Evaluation of the EBs in terms of morphology and potential to differentiate indicated that they were typical in structure and could generate various cell types;they were also able to form into tissue-like structures.Moreover,with introduction of ascorbic acid,ES cells differentiated into cardiomyocytes efficiently and started contracting synchronously at day 19.The results demonstrated that collagen/Matrigel scaffolds supported EBs formation and their subsequent differentiation in a single three dimensional environment. | Jin Zhou Ye Zhang Qiuxia Lin Zhiqiang Liu Haibin Wang Cuimi Duan Yanmeng Wang Tong Hao Kmwu Wu Changyong Wang | 2010 | Journal of Genetics and Genomics2010，37，7： | 4 |
| 2 | Distribution and characteristics of telocytes as nurse cells in the architectural organization of engineered heart tissues显示文摘Interstitial Cajal-like cells are a distinct type of interstitial cell with a wide distribution in mammalian organs and tissues,and have been given the name'telocytes'.Recent studies have demonstrated the potential roles of telocytes in heart development,renewal,and repair.However,further research on the functions of telocytes is limited by the complicated in vivo environment.This study was designed to construct engineered heart tissue(EHT)as a three-dimensional model in vitro to better understand the role of telocytes in the architectural organization of the myocardium.EHTs were constructed by seeding neonatal cardiomyocytes in collagen/Matrigel scaffolds followed by culture under persistent static stretch.Telocytes in EHTs were identified by histology,toluidine blue staining,immunofluorescence,and transmission electron microscopy.The results from histology and toluidine blue staining demonstrated widespread putative telocytes with compact toluidine blue-stained nuclei,which were located around cardiomyocytes.Prolongations from the cell bodies showed a characteristic dichotomous branching pattern and formed networks in EHTs.Immunofluorescence revealed positive staining of telocytes for CD34 and vimentin with typical moniliform prolongations.A series of electron microscopy images further showed that typical telocytes embraced the cardiomyocytes with their long prolongations and exhibited a marked appearance of nursing cardiomyocytes during the construction of EHTs.This finding highlights the great importance of telocytes in the architectural organization of EHTs.It also suggests that EHT is an appropriate physical and pathological model system in vitro to study the roles of telocytes during heart development and regeneration. | ZHOU Jin WANG Yan ZHU Ping SUN HongYu MOU YongChao DUAN CuiMi YAO AnNing LV ShuangHong WANG ChangYong | 2014 | Science China(Life Sciences)2014，57，2： | 4 |
| 3 | Promotion of cardiac differentiation of brown adipose derived stem cells by chitosan hydrogel for repair after myocardial infarction显示文摘 | Haibin Wang Jinxin Shi Yan Wang Yujing Yin Liman Wang Jianfeng Liu Zhiqiang Liu Cuimi Duan Ping Zhu Changyong Wang | 2014 | Biomaterials2014，，： | 2 |
| 4 | In vivo chondrogenesis of adult bone-marrow-derived autologous mesenchymal stem cells显示文摘 | Jinwu Chen Changyong Wang Shuanghong Lü Junzheng Wu Ximin Guo Cuimi Duan Lingzhi Dong Ying Song Junchuan Zhang Dianying Jing Linbo Wu Jiandong Ding Dexue Li | 2005 | Cell and Tissue Research2005，，3： | 1 |
| 5 | A chitosan–glutathione based injectable hydrogel for suppression of oxidative stress damage in cardiomyocytes显示文摘 | Junjie Li Yao Shu Tong Hao Yan Wang Yufeng Qian Cuimi Duan Hongyu Sun Qiuxia Lin Changyong Wang | 2013 | Biomaterials2013，，36： | 1 |
| 6 | The influence of chitosan hydrogel on stem cell engraftment, survival and homing in the ischemic myocardial microenvironment显示文摘 | Zhiqiang Liu Haibin Wang Yan Wang Qiuxia Lin Anning Yao Feng Cao Dexue Li Jin Zhou Cuimi Duan Zhiyan Du Yanmeng Wang Changyong Wang | 2011 | Biomaterials2011，，11： | 1 |
| 7 | Improved myocardial performance in infarcted rat heart by co-injection of basic fibroblast growth factor with temperature-responsive Chitosan hydrogel显示文摘 | Haibin Wang Xuelian Zhang Yanmin Li Yitong Ma Ye Zhang Zhiqiang Liu Jin Zhou Qiuxia Lin Yanmeng Wang Cuimi Duan Changyong Wang | 2010 | Journal of Heart and Lung Transplantation2010，，8： | 1 |
| 8 | Cardiac patches made of brown adipose-derived stem cell sheets and conductive electrospun nanofibers restore infarcted heart for ischemic myocardial infarction显示文摘Cell sheet engineering has been proven to be a promising strategy for cardiac remodeling post-myocardial infarction. However, insufficient mechanical strength and low cell retention lead to limited therapeutic efficiency. The thickness and area of artificial cardiac patches also affect their therapeutic efficiency. Cardiac patches prepared by combining cell sheets with electrospun nanofibers, which can be transplanted and sutured to the surface of the infarcted heart, promise to solve this problem. Here, we fabricated a novel cardiac patch by stacking brown adipose-derived stem cells (BADSCs) sheet layer by layer, and then they were combined with multi-walled carbon nanotubes (CNTs)-containing electrospun polycaprolactone/silk fibroin nanofibers (CPSN). The results demonstrated that BADSCs tended to generate myocardium-like structures seeded on CPSN. Compared with BADSCs suspension-containing electrospun nanofibers, the transplantation of the CPSN-BADSCs sheets (CNBS) cardiac patches exhibited accelerated angiogenesis and decreased inflammation in a rat myocardial infarction model. In addition, the CNBS cardiac patches could regulate macrophage polarization and promote gap junction remodeling, thus restoring cardiac functions. Overall, the hybrid cardiac patches made of electrospun nanofibers and cell sheets provide a novel solution to cardiac remodeling after ischemic myocardial infarction. | Xinbo Wei Li Wang Cuimi Duan Kai Chen Xia Li Ximin Guo Peng Chen Haifeng Liu Yubo Fan | 2023 | Bioactive Materials2023，，9： | 0 |
| 9 | Identification and characterization of mammaglobin-A epitope in heterogenous breast cancers for enhancing tumor-targeting therapy显示文摘Although targeted therapy has been extensively investigated for breast cancers,a molecular target with broad application is currently unavailable due to the high heterogeneity of these cancers.Mammaglobin-A(Mam-A),which is overexpressed in most breast carcinomas,has been proposed as a promising target.However,the lack of specific targeting moieties due to uncertain binding epitopes hampers further translational study.Here,seven potential epitopes of Mam-A were disclosed,and a unique epitope was then identified in most types of breast cancers,despite the genotypic heterogeneity.With phage display technology,the epitope was determined to be N-terminal amino acids 42–51 of Mam-A(N42–51).Then,the N42–51 epitope-specific monoclonal antibody,mAb785,was conjugated to poly lactic-co-glycolic acid(PLGA)nanoparticles loaded with therapeutic agents,thereby enhancing the drug uptake and therapeutic efficacy in different genotypes of breast cancers.The computer simulation of the N42–51 epitope and the mAb785 structures,as well as their interactions,further revealed the specific targeting mechanism of the mAb785-conjugated nanoparticles to breast cancers. | Zhiqiang Liu Xiqin Yang Cuimi Duan Jiangxue Li Rongsheng Tong Yuting Fan Jiannan Feng Ruiyuan Cao Wu Zhong Xiaoyan Feng Heqiu Zhang Lulu Cai | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 0 |
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