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| 1 | The serrated pathway to colorectal carcinoma: current concepts and challenges显示文摘 | Mark Bettington Neal Walker Andrew Clouston Ian Brown Barbara Leggett Vicki Whitehall | 2013 | Histopathology2013，，3： | 6 |
| 2 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012，18，15： | 3 |
| 3 | Critical Appraisal of the Diagnosis of the Sessile Serrated Adenoma显示文摘 | Mark Bettington Neal Walker Christophe Rosty Ian Brown Andrew Clouston Leesa Wockner Vicki Whitehall Barbara Leggett | 2014 | The American Journal of Surgical Pathology2014，，2： | 2 |
| 4 | Senescent human hepatocytes express a unique secretory phenotype and promote macrophage migration显示文摘AIM:To develop a model of stress-induced senescence to study the hepatocyte senescence associated secretory phenotype(SASP).METHODS:Hydrogen peroxide treatment was used to induce senescence in the human Hep G2 hepatocyte cell line.Senescence was confirmed by cytochemical staining for a panel of markers including Ki67,p21,heterochromatin protein 1β,and senescence-associated-β-galactosidase activity.Senescent hepatocytes were characterised by gene expression arrays and quantitative polymerase chain reaction(q PCR),and conditioned media was used in proteomic analyses,a human chemokine protein array,and cell migration assays to characterise the composition and function of the hepatocyte SASP.RESULTS:Senescent hepatocytes induced classical markers of senescence(p21,heterochromatin protein1β,and senescence-associated-β-galactosidase activity);and downregulated the proliferation marker,Ki67.Hepatocyte senescence induced a 4.6-fold increase in total secreted protein(P=0.06)without major alterations in the protein profile.Senescence-induced genes were identified by microarray(Benjamini Hochbergcorrected P<0.05);and,consistent with the increase in secreted protein,gene ontology analysis revealed a significant enrichment of secreted proteins among inducible genes.The hepatocyte SASP included characteristic factors such as interleukin(IL)-8 and IL-6,as well as novel components such as SAA4,IL-32and Fibrinogen,which were validated by q PCR and/or chemokine protein array.Senescent hepatocyteconditioned medium elicited migration of inflammatory(granulocyte-macrophage colony stimulating factor,GM-CSF-derived),but not non-inflammatory(CSF-1-derived)human macrophages(P=0.022),which could contribute to a pro-inflammatory microenvironment in vivo,or facilitate the clearance of senescent cells.CONCLUSION:Our novel model of hepatocyte senescence provides insights into mechanisms by which senescent hepatocytes may promote chronic liver disease pathogenesis. | Katharine M Irvine Richard Skoien Nilesh J Bokil Michelle Melino Gethin P Thomas Dorothy Loo Brian Gabrielli Michelle M Hill Matthew J Sweet Andrew D Clouston Elizabeth E Powell | 2014 | World Journal of Gastroenterology2014，20，47： | 2 |
| 5 | Progressive Fibrosis in Nonalcoholic Steatohepatitis: Association With Altered Regeneration and a Ductular Reaction显示文摘 | Michelle M. Richardson Julie R. Jonsson Elizabeth E. Powell Elizabeth M. Brunt Brent A. Neuschwander–Tetri Prithi S. Bhathal John B. Dixon Martin D. Weltman Herbert Tilg Alexander R. Moschen David M. Purdie Anthony J. Demetris Andrew D. Clouston | 2007 | Gastroenterology2007，，1： | 2 |
| 6 | 支持完整性决策的数据管理显示文摘大多数管道的运营、维护和其它设备管理部门,采用一系列各自独立的数据资源和应用程序来管理管道的完整性、维修和安全。将各个独立的数据整合成一个单一的、相互关联的数据管理系统可以提供很多重要的好处。然而,在给定管道早期投资的情况下,评价用于建立一个包含功能强大的数据采集程序在内的新系统的成本是非常困难的。采用静态的和独立的维护性数据的专用风险管理软件被作为一种快捷的、低成本的替代选择,以此来满足规章制度所规定的必须遵从的行业要求。通过开发传统数据等方法,以最小的启动成本来实现阶段性的完整性数据管理。 | John Beets Ed Wiegele Stuart Clouston | 2005 | 油气储运2005，24，B12： | 2 |
| 7 | The pattew of glomerular disease in New Caledonia:Preliminary finding显示文摘 | Painter D Clouston D Ahn E | 1996 | Pathology1996，28，10： | 1 |
| 8 | Timely topic: premalignant lesions associated with ad-enoearcinoma of the upper gastrointestinal tract 显示文摘 | Clouston AD | 2001 | Pathology2001，33，3： | 1 |
| 9 | Angiotensin-converting enzyme inhibition attenuates the progression of rat hepatic fibrosis显示文摘 | Jonsson JR Clouston AD Ando Y | 2001 | Gastroenterology2001，121，1： | 1 |
| 10 | Angiotensin convering enzyme inhibition ate- nuates the progression of rat hepatic fibrosis 显示文摘 | Johsson JR Clouston AD Ando Y | 2001 | Gastroenterlogy2001，121，1： | 1 |
| 11 | The serrated pathway to colorectal carcinoma : culxent concepts and challenges 显示文摘 | Bettington M Walker N Clouston A | 2013 | Histopa- thology2013，62，3： | 1 |
| 12 | Black Cohosh and other Jherbal remedies associated with acute hepatitis 显示文摘 | Whiting PW Clouston A Kerlin P | 2002 | Med Aust2002，177，8： | 1 |
| 13 | Steatosis in chronic hepatitis C:association with increased messenger RNA expression of collagen I,tumor necrosis factor-alphs and cytochrome P450 2E1显示文摘 | Gochee PA Jonsson JR Clouston AD | 2003 | J Gastroenterol Hepatol2003，18，： | 1 |
| 14 | Cyclosporin A pretreatment in a rat model of warm ischaemia/reperfusion inury显示文摘 | Saxton NE Barclay JL Clouston AD Fawcett J | | 0，，： | 1 |
| 15 | Steatosis as a co-factor for other diseases显示文摘 | Powell EE Jnnsson JR Clouston AD | | 0，，： | 1 |
| 16 | Angiotensin - converting enzyme inhibition attenuates the progression of rat hepatic fibrosis显示文摘 | Jonsson JR Clouston AD Ando Y | 2001 | Gastroenterology2001，121，1： | 1 |
| 17 | Intrdarterial papaverine infusion for cerebral vasospasm after subarachnoid hemorrbaage显示文摘 | Clouston J E Numaguchi Y Zoarske C H | 1993 | AJNR1993，16，： | 1 |
| 18 | Steatosis: co-factor in other liver diseases 显示文摘 | Powell EE Jonsson JR Clouston AD | 2005 | Hepatology2005，42，1： | 1 |
| 19 | Independent effects of diet and exercise training on fat oxidation in non-alcoholic fatty liver disease显示文摘AIM To investigate the independent effects of 6-mo of dietary energy restriction or exercise training on wholebody and hepatic fat oxidation of patients with nonalcoholic fatty liver disease(NAFLD).METHODS Participants were randomised into either circuit exercise training(EX;n = 13;3 h/wk without changes in dietary habits),or dietary energy restriction(ER) without changes in structured physical activity(ER;n = 8).Respiratory quotient(RQ) and whole-body fat oxidation rates(Fatox) were determined by indirect calorimetry under basal,insulin-stimulated and exercise conditions.Severity of disease and steatosis was determined by liver histology;hepatic Fatox was estimated from plasma β-hydroxybutyrate co.ncentrations;cardiorespiratory fitness was expressed as VO2 peak.Complete-case analysis was performed(EX:n = 10;ER:n = 6).RESULTS Hepatic steatosis and NAFLD activity score decreased with ER but not with EX.β-hydroxybutyrate concentrations increased significantly in response to ER(0.08 ± 0.02 mmol/L vs 0.12 ± 0.04 mmol/L,P = 0.03) but remained unchanged in response to EX(0.10 ± 0.03 mmol/L vs 0.11 ± 0.07 mmol/L,P = 0.39).Basal RQ decreased(P = 0.05) in response.to EX,while this change was not significant after ER(P = 0.38).VO_(2peak)(P < 0.001) and maximal Fa_(tox) during aerobic exercise(P = 0.03) improved with EX but not with ER(P > 0.05).The increase in β-hydroxybutyrate concentrations was correlated with the reduction in hepatic steatosis(r =-0.56,P = 0.04).CONCLUSION ER and EX lead to specific benefits on fat metabolism of patients with NAFLD.Increased hepatic Fat_(ox) in response to ER could be one mechanism through which the ER group achieved reduction in steatosis. | Ilaria Croci Nuala M Byrne Veronique S Chachay Andrew P Hills Andrew D Clouston Trisha M O'Moore-Sullivan Johannes B Prins Graeme A Macdonald Ingrid J Hickman | 2016 | World Journal of Hepatology2016，8，27： | 1 |
| 20 | Intraarterial papaverine infusion for cerebral vasospasm after subarachnoid hemorrhage 显示文摘 | Numaguchi Y Zoarski GH Aldrich BF Simard JM Zitnay KM | 1995 | AJNR Am J Neuroradiol1995，16，： | 1 |
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