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| 1 | 2019新型冠状病毒基因组特征和流行病学:病毒起源和受体结合的意义显示文摘研究者对来自9例新型冠状病毒肺炎住院患者的支气管肺泡灌洗液样本和培养的分离株进行了下一代测序。从这些个体中获得了严重急性呼吸综合征-冠状病毒2(severe acute respiratory syndrome-coronavirus 2,SARS-CoV-2)的完整和部分基因组序列。利用Sanger测序连接病毒重叠群以获得全长基因组,cDNA末端快速扩增确定终端区。对这些SARSCoV-2基因组和其他冠状病毒基因组进行了系统进化分析,以确定该病毒的进化史并有助于推断其可能的起源。 | 刘青(译) 刘莉(审校) Lu R Zhao X Li J Niu P Yang B Wu H Wang W Song H Huang B Zhu N Bi Y Ma X Zhan F Wang L Hu T Zhou H Hu Z Zhou W Zhao L Chen J Meng Y Wang J Lin Y Yuan J Xie Z Ma J Liu WJ Wang D Xu W Holmes EC Gao GF Wu G Chen W Shi W Tan W | 2020 | 中华高血压杂志2020，28，3： | 516 |
| 2 | Black carbon aerosols and their radiative properties in the Pearl River Delta region显示文摘The climatic and environmental effects of atmospheric aerosols are a hot topic in global science community, and radiative properties of the aerosols are one of the important parameters in assessing climatic change. Here we studied the black carbon concentration and absorption coefficient measured with aethalometers, scattering coefficient measured with nephelometers, and single scattering albedo derived at an atmospheric composition watch station in Guangzhou from 2004 to 2007. Our main results are as follows. The data of black carbon concentration and absorption coefficients measured with instruments cannot be directly used until they are measured in parallel with internationally accepted instruments for comparison, calibration, and reduction. After evaluation of the data, the result shows that the monthly mean of BC concentration varies 3.1―14.8 μg·m-3 and the concentration decreases by about 1 μg·m-3 in average over the four years; It is higher in the dry season with a multi-year mean of 8.9 μg/m3 and lower in the rainy season with a multi-year mean of 8.0 μg·m-3; The extreme maximum of monthly mean concentration occurred in December 2004 and extreme minimum in July 2007, and a 4-year mean is 8.4 μg·m-3. It is also shown that monthly mean scattering coefficient derived varies 129 -565 Mm-1, monthly mean absorption coefficient 32-139 Mm-1, and monthly mean single scattering albedo 0.71-0.91, with annual mean values of 0.80, 0.82, 0.79 and 0.84 for 2004, 2005, 2006 and 2007, respectively. Three instruments were used to take simultaneous measurements of BC in PM10, PM2.5, and PM1 and the results showed that PM2.5 took up about 90% of PM10 and PM1 accounted for about 68% of PM2.5, and BC aerosols are mainly present in fine particulates. The variability of BC concentrations is quite consistent between the Nancun station (141 m above sea level) and the Panyu station (13 m above sea level), which are 8 km apart from each other. The concentration in higher altitude station (Panyu) is consistently lower than the lower altitude station (Nancun), and the difference of annual mean is about 4 μg·m-3. | WU Dui MAO JieTai DENG XueJiao TIE XueXi ZHANG YuanHang ZENG LiMin LI Fei TAN HaoBo BI XueYan HUANG XiaoYing CHEN Jing DENG Tao | 2009 | Science China Earth Sciences2009，52，8： | 58 |
| 3 | Identification and expansion of cancer stem cells in tumor tissues and peripheral blood derived from gastric adenocarcinoma patients显示文摘胃的癌症是世界范围的第四很普通的癌症,与死亡的高率和低 5 年的幸存率。迄今为止,有为胃的癌症的有效治疗学的协议的缺乏。最近的研究建议癌症干细胞(CSC ) 为肿瘤开始,侵略,转移,和抵抗负责到 anticancer 治疗。因此,指向胃的 CSC 的治疗是吸引人的。然而,在人的胃的腺癌(GAC ) 的 CSC 没被描述。这里,我们从 GAC 病人在肿瘤纸巾和外部血识别 CSC。从肿瘤纸巾和病人的外部血被孤立的人的 GAC (GCSC ) 的 CSC 带了 CD44 和 CD54 表面标记,当注入了 immunodeficient 老鼠时,高度类似于原来的人的肿瘤的产生肿瘤,在 vitro 区分了进胃的上皮的房间,并且在 vivo 并且在 vitro 自我更新。我们的调查结果建议有效治疗学的协议必须指向 GCSC。从 GAC 病人的循环的 GCSC 的俘获也为为肿瘤转移潜在地负责的一张批评房间人口的鉴定显示出大潜力,并且为早诊断并且胃的癌症的纵的监视提供一个有效协议。 | Tie Chen Kun Yang Jianhua Yu Wentong Meng Dandan Yuan Feng Bi Fang Liu Jie Liu Bing Dai Xinzu Chen Fang Wang Fan Zeng Hong Xu Jiankun Hu Xianming Mo | 2012 | Cell Research2012，22，1： | 50 |
| 4 | Assessment of heavy metal pollution characteristics and human health risk of exposure to ambient PM_(2.5) in Tianjin,China显示文摘To examine the features of heavy metal pollution of PM_(2.5)(particulate matter less than 2.5 μm) in Tianjin,China,as well as the exposure risk of PM_(2.5) to human health,we analyzed ambient PM_(2.5) samples collected from a campus of Nankai University in June,August,and October 2012.The concentrations of PM_(2.5) and heavy metals(Ni,Cu,Pb,Zn,Cr,Cd,Hg,As and Mn) in PM2.5 were analyzed by gravimetric analysis and inductively coupled plasma-mass spectrometry,respectively.The results show that the heavy metals contained in PM_(2.5) were,in descending order,Cu,Zn,Pb,Mn,Cr,Ni,Cd,As,and Hg.The proportion of Cd exceeded the secondary level of National Ambient Air Quality Standard of China(GB 3095-2012) by 1.3times,while others were within the limit.Enrichment factor analysis indicated that Cu,Zn,Cd,Pb,and Hg are mainly from anthropogenic sources.Principal component analysis indicated that the main sources of the heavy metals are vehicle exhaust,chemical waste,and coal-burning activities.The nine heavy metals which may cause health issues by exposure through the human respiratory system and should be further examined are Cr,Cd,As,Ni,Cu,Pb,Mn,Zn,and Hg,in the order of decreasing risk levels.With reference to the U.S.EPA standard the risk levels of all nine metals were below the acceptable level(10^(-6)/year). | Peifei Chen Xiaohui Bi Jiaqi Zhang Jianhui Wu Yinchang Feng | 2015 | Particuology2015，13，3： | 43 |
| 5 | Erianin,a novel dibenzyl compound in Dendrobium extract,inhibits lung cancer cell growth and migration via calcium/calmodulin-dependent ferroptosis显示文摘Ferroptosis,a novel form of programmed cell death,is characterized by iron-dependent lipid peroxidation and has been shown to be involved in multiple diseases,including cancer.Stimulating ferroptosis in cancer cells may be a potential strategy for cancer therapy.Therefore,ferroptosis-inducing drugs are attracting more attention for cancer treatment.Here,we showed that erianin,a natural product isolated from Dendrobium chrysotoxum Lindl,exerted its anticancer activity by inducing cell death and inhibiting cell migration in lung cancer cells.Subsequently,we demonstrated for the first time that erianin induced ferroptotic cell death in lung cancer cells,which was accompanied by ROS accumulation,lipid peroxidation,and GSH depletion.The ferroptosis inhibitors Fer-1 and Lip-1 but not Z-VAD-FMK,CQ,or necrostatin-1 rescued erianin-induced cell death,indicating that ferroptosis contributed to erianin-induced cell death.Furthermore,we demonstrated that Ca^(2+)/CaM signaling was a critical mediator of erianin-induced ferroptosis and that blockade of this signaling significantly rescued cell death induced by erianin treatment by suppressing ferroptosis.Taken together,our data suggest that the natural product erianin exerts its anticancer effects by inducing Ca^(2+)/CaMdependent ferroptosis and inhibiting cell migration,and erianin will hopefully serve as a prospective compound for lung cancer treatment. | Peng Chen Qibiao Wu Jiao Feng Lili Yan Yitian Sun Shuiping Liu Yu Xiang Mingming Zhang Ting Pan Xiaying Chen Ting Duan Lijuan Zhai Bingtao Zhai Wengang Wang Ruonan Zhang Bi Chen Xuemeng Han Yicong Li Liuxi Chen Ying Liu Xingxing Huang Ting Jin Wenzheng Zhang Hong Luo Xiaohui Chen Yongqiang Li Qiujie Li Guohua Li Qin Zhang Lvjia Zhuo Zuyi Yang Huifen Tang Tian Xie Xiaoping Ouyang Xinbing Sui | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 42 |
| 6 | MEKK1, MKK1/MKK2 and MPK4 function together in a mitogen-activated protein kinase cascade to regulate innate immunity in plants显示文摘激活 Mitogen 的蛋白质 kinase (MAPK ) 串联在调整植物起重要作用天生的有免疫力的回答。在到为有组成的防卫回答的异种的搜索的一幅基因屏幕,我们识别了展出房间死亡和组成的防卫回答的 mpk4 和 mekk1 的多重等位基因。双分子的荧光互补(BiFC ) 分析证明 MPK4 和 MEKK1 与 MKK1 和 MKK2 交往,二密切相关的 MAPK kinases。mkk1 和 mkk2 单身者异种植物没有明显的变异的显型。测试 MKK1 和 MKK2 是否冗余地工作, mkk1 mkk2 两倍异种被产生。mkk1 mkk2 两倍变异的植物在幼苗舞台和幼苗致命性显型死是温度依赖者。类似于 mpk4 和 mekk1 异种,两倍变异的幼苗积累的 mkk1 mkk2 H2O2 高级,显示自发的房间死亡,联系的组成地快速的致病(PR ) 基因和展览病原体抵抗。另外,由 flg22 的 MPK4 的激活在 mkk1 mkk2 被损害两倍异种,在地图 kinase 串联和 MPK4 和 MEKK1 建议那 MKK1 和 MKK2 功能否定地在植物调整天生的有免疫力的回答。 | Minghui Gao Jinman Liu Dongling Bi Zhibin Zhang Fang Cheng2, Sanfeng Chen Yuelin Zhang | 2008 | Cell Research2008，18，12： | 37 |
| 7 | Upregulation of PTEN involved in rosiglitazone-induced apoptosis in human hepatocellular carcinoma cells显示文摘瞄准:调查 rosiglitazone 的效果, peroxisomeproliferator-ac-tivated 受体 gamma ( PPARy )收缩筋在在 hepatocellular carcinomacells 在染色体 10 基因( PTEN )和房间生长上删除的磷酸酶 andtensin 相当或相同事物的表示上,象这些 effects.Methods 的内在的机制一样: RT-PCR 和西方的 blottinganalyses 被执行在 Hep3B 房间 treatedwith rosiglitazone.3-( 4,5-Dimethylthiazol-2-yl )检测抄写和 PTEN 的表示 -2,5-diphenyl | Liang-qi CAO Xi-lin CHEN Qian WANG Xiao-hui HUANG Mao-chuan ZHEN Long-juan ZHANG Wen LI Jiong BI | 2007 | Acta Pharmacologica Sinica2007，28，6： | 32 |
| 8 | Morphological study on colonic pathology in ulcerative colitis treated by moxibustion显示文摘AIM To observe the therapeutic effect ofmoxibustion on ulcerative colitis and itsinfluence on the colonic mucosal morphology.METHODS Forty-six patients with ulcerativecolitis were randomly divided into themoxibustion with herbal medicine underneathgroup and the western medicine group.Thirtypatients were treated with the abovemoxibustion and 16 patients with Salicylayefapyridine(SASP).The colonic mucosa of 13patients in the moxibustion group was observedby colonoscopy before and after the treatment.Mucin was also analyzed by H.E and AB-PASstaining.RESULTS Seventeen patients were clinicallycured,12 were improved and 1 unchanged in themoxibustion group.In the control group,5patients were clinically cured,7 improved and 4unchanged.Thirteen patients with active UCwere taken as the subjects for histopathologicanalysis in this study.The colonic mucosallesions were remarkably improved and thecharacteristic of the mucin also changed.Inmost sections,the chronic inflammation ofmucosa was geatly ameliorated(P<0.01).Theinflammatory cell infiltratation much decreased and neutrophils,disapeared in most sections(P<0.001).The goblet cells significantlyincreased(P<0.001);crypt paracrypt abscessor mucosal ulceration was seen(P<0.001).CONCLUSION The rate of cure of ulcerative colitis by moxibustion with herbal medicine beneath is superior to that by SASP. This sort of moxibustion can effectively improve the colonic mucosal lesions and restore the proportion of mucoprotein to near normal. | Huan Gan Wu Li Bin Zhou Da Ren Shi Shi Min Liu Hui Rong Liu Bi Meng Zhang Han Ping Chen Lin Shan Zhang | 2000 | World Journal of Gastroenterology2000，6，6： | 28 |
| 9 | Postharvest Treatment with β-Aminobutyric Acid Induces Resistance Against Dry Rot Caused by Fusarium sulphureum in Potato Tuber显示文摘The effectiveness of postharvest β-aminobutyric acid (BABA) treatment was studied for inducing resistance against dry rot caused by Fusarium sulphureum in tubers and slices of two potato cultivars (resistant cultivar Shepody and susceptible cultivar Xindaping). The results showed that BABA at 100 mmol L-1 significantly reduced lesion diameter in inoculated both tubers and slices. The chemical at 100 mmol L-1 showed an effective reduction in infection ability of F. sulphureum inoculated 48 and 72 h after treatment in slices of resistant cultivar, and 72 and 96 h in susceptible ones. BABA increased the activitives of peroxidase (POD), polyphenoloxidase (PPO) and phenylalanine ammonialyase (PAL), and accumulated the contents of lignin, flavonoids and phenolics in slices. The resistant cultivar had a stronger resistant response than the susceptible one. These findings suggest that the BABA treatment can induce the resistance in potato tubers, however, the inducing degree depends on the original level of resistance present in each cultivar. | YIN Yan LI Yong-cai BI Yang CHEN Song-jiang LI Ying-chao YUAN Li WANG Yi WANG Di | 2010 | Agricultural Sciences in China2010，9，9： | 24 |
| 10 | Schisandrol B protects against acetaminophen- induced acute hepatotoxicity in mice via activation of the NRF2/ARE signaling pathway显示文摘目的: 原子因素 erythroid 2-related 因素(NRF2 ) 2 通过抗氧化剂反应元素行动() 处理调整许多除去和为 cytoprotective 负责的抗氧化剂基因的表示。我们以前报导从五味子 sphenanthera 孤立的 Schisandrol B (SolB ) 对导致的 acetaminophen (APAP ) 生产了保护的效果肝损害。在这研究,我们调查了表明小径的 NRF2/ARE 是否涉及这 hepato 保护的 effect.Methods : 男 C57BL/6 老鼠与 SolB 被对待(200 mg· kg −1·d−1, ig ) 为在 APAP 的注射前的 3 d (400 mg/kg, ip ) 。浆液和肝织物样品被收集 6 h 以后。mRNA 和蛋白质表示分别地用 qRT-PCR 和西方的污点试金被测量。 NRF2 的激活用酶记者基因 assay.Results 在 HepG2 房间被检验:显著地减轻的 SolB 预告的处理肝的损害(肝的湾穴的大补缀的坏死和 hyperemia ),浆液著名计算机生产厂商的增加,中高音层次和肝的 MDA 满足,并且肝和 mitochondrial 谷胱甘肽的减少在 对待APAP 的老鼠铺平。而且, SolB 预告的处理显著地增加了 NRF2 的原子累积并且增加了 NRF2 下游的蛋白质的肝的表示,包括在对待 APAP 的鼠标的 GCLC, GSR, NQO1, GST, MRP2, MRP3 和 MRP4。而且,有 SolB 的处理(2.5-20 μ mol/L ) dose-dependently 在 HepG2 cells.Conclusion 增加了 NRF2 记者基因的活动: SolB 对导致 APAP 的 hepatotoxicity 展出显著保护的效果,部分,经由 NRF2/ARE 的激活, NRF2 的小径和规定指向基因,它导致 detoxification 和增加抗氧化剂能力。 | Yi-ming JIANG Ying WANG Hua-sen TAN Tao YU Xiao-mei FAN Pan CHEN Hang ZENG Min HUANG Hui-chang BI | 2016 | Acta Pharmacologica Sinica2016，37，3： | 24 |
| 11 | Chitosan conduits combined with nerve growth factor microspheres repair facial nerve defects显示文摘Microspheres containing nerve growth factor for sustained release were prepared by a compound method,and implanted into chitosan conduits to repair 10-mm defects on the right buccal branches of the facial nerve in rabbits.In addition,chitosan conduits combined with nerve growth factor or normal saline,as well as autologous nerve,were used as controls.At 90 days post-surgery,the muscular atrophy on the right upper lip was more evident in the nerve growth factor and normal saline groups than in the nerve growth factor-microspheres and autologous nerve groups.Electrophysiological analysis revealed that the nerve conduction velocity and amplitude were significantly higher in the nerve growth factor-microspheres and autologous nerve groups than in the nerve growth factor and normal saline groups.Moreover,histological observation illustrated that the diameter,number,alignment and myelin sheath thickness of myelinated nerves derived from rabbits were higher in the nerve growth factor-microspheres and autologous nerve groups than in the nerve growth factor and normal saline groups.These findings indicate that chitosan nerve conduits combined with microspheres for sustained release of nerve growth factor can significantly improve facial nerve defect repair in rabbits. | Huawei Liu Weisheng Wen Min Hu Wenting Bi Lijie Chen Sanxia Liu Peng Chen Xinying Tan | 2013 | Neural Regeneration Research2013，8，33： | 22 |
| 12 | Differentiation of isolated human umbilical cord mesenchymal stem cells into neural stem cells显示文摘AIM:To investigate whether umbilical cord human mesenchymal stem cell(UC-MSC)was able to differentiate into neural stem cell and neuron in vitro.·METHODS:The umbilical cords were obtained from pregnant women with their written consent and the approval of the Clinic Ethnics Committee.UC-MSC were isolated by adherent culture in the medium contains 20%fetal bovine serum(FBS),then they were maintained in the medium contain 10%FBS and induced to neural cells in neural differentiation medium.We investigated whether UC-MSC was able to differentiate into neural stem cell and neuron in vitro by using flow cytometry,reverse transcriptase-polymerase chain reaction(RT-PCR)and immunofluorescence(IF)analyzes.·RESULTS:A substantial number of UC-MSC was harvested using the tissue explants adherent method at about 2wk.Flow cytometric study revealed that these cells expressed common markers of MSCs,such as CD105(SH2),CD73(SH3)and CD90.After induction of differentiation of neural stem cells,the cells began to form clusters;RT-PCR and IF showed that the neuron specific enolase(NSE)and neurogenic differentiation 1-positive cells reached 87.3%±14.7%and 72.6%±11.8%,respectively.Cells showed neuronal cell differentiation after induced,including neuron-like protrusions,plump cell body,obviously and stronger refraction.RT-PCR and IF analysis showed that microtubule-associated protein 2(MAP2)and nuclear factor-M-positive cells reached 43.1%±10.3%and 69.4%±19.5%,respectively.·CONCLUSION:Human umbilical cord derived MSCscan be cultured and proliferated in vitro and differentiate into neural stem cells,which may be a valuable source for cell therapy of neurodegenerative eye diseases. | Song Chen Wei Zhang Ji-Ming Wang Hong-Tao Duan Jia-Hui Kong Yue-Xin Wang Meng Dong Xue Bi Jian Song | 2016 | International Journal of Ophthalmology(English edition)2016，9，1： | 22 |
| 13 | Baicalin induces ferroptosis in bladder cancer cells by downregulating FTH1显示文摘Ferroptosis is a non-apoptotic regulated cell death caused by iron accumulation and subsequent lipid peroxidation.Currently,the therapeutic role of ferroptosis on cancer is gaining increasing interest.Baicalin an active component in Scutellaria baicalensis Georgi with anticancer potential various cancer types;however,the effects of baicalein on bladder cancer and the underlying molecular mechanisms remain largely unknown.In the study,we investigated the effect of baicalin on bladder cancer cells5637 and KU-19-19.As a result,we show baicalin exerted its anticancer activity by inducing apoptosis and cell death in bladder cancer cells.Subsequently,we for the first time demonstrate baicalin-induced ferroptotic cell death in vitro and in vivo,accompanied by reactive oxygen species(ROS) accumulation and intracellular chelate iron enrichment.The ferroptosis inhibitor deferoxamine but not necrostatin-1,chloroquine(CQ),N-acetyl-L-cysteine,L-glutathione reduced,or carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone(Z-VAD-FMK) rescued baicalin-induced cell death,indicating ferroptosis contributed to baicalin-induced cell death.Mechanistically,we show that ferritin heavy chain1(FTH1) was a key determinant for baicalin-induced ferroptosis.Overexpression of FTH1 abrogated the anticancer effects of baicalin in both 5637 and KU19-19 cells.Taken together,our data for the first time suggest that the natural product baicalin exerts its anticancer activity by inducing FTH1-dependent ferroptosis,which will hopefully provide a prospective compound for bladder cancer treatment. | Na Kong Xiaying Chen Jiao Feng Ting Duan Shuiping Liu Xueni Sun Peng Chen Ting Pan Lili Yan Ting Jin Yu Xiang Quan Gao Chengyong Wen Weirui Ma Wencheng Liu Mingming Zhang Zuyi Yang Wengang Wang Ruonan Zhang Bi Chen Tian Xie Xinbing Sui Wei Tao | 2021 | Acta Pharmaceutica Sinica B2021，11，12： | 23 |
| 14 | Effects of precipitation and landuse on runoff during the past 50 years in a typical watershed in the Loess Plateau, China显示文摘In the past century, great progress has been made worldwide in our understanding of forest-water relationship. The successful forestation programs implemented in China-which have improved the ecological environmental conditions-have gained the attention of many researchers and highlighted the relationship between forestation and water yields. The arid and semi-arid Loess Plateau has received attention from water engineers and eco-hydrological researchers in China because of a shortage in water resources. We selected one of the oldest stations conducting soil and water conservation experiments, the Xifeng soil and water conservation station, and chose the Nanxiaohe catchment and its paired catchments (Yangjiagou catchment and Dongzhuanggou catchment) as our research areas. Trends in precipitation, air temperature, streamflow over the past 50 years, and the effect of changing land use on streamflow were analyzed. The Mann-Kendall test showed that precipitation had a negative trend (downward trend), whereas air temperature showed a positive trend (upward trend) from the past to present in the Nanxiaohe catchment. However, the trends seen in precipitation, air temperature did not contain any 'jumping points.' The paired catchment approach is used to detect the effects of land cover change on hydrology in the Yangjiagou and the contrast catchment, i.e., Dongzhuanggou catchment in our study. The results showed a large change in land use in the Yangjiagou catchment from 1954 to 2008. An increase in forested land (from 0% to 40.08% from 1954 to 2008) and a reduction of bare land (from 51.26% to 5.50% from 1954 to 2008) accounted for a large part of the change in land use. However, the land use changed little in the contrast catchment. The comparison of streamflow in the paired catchments showed that forestation reduced streamflow by 49.63% (or 6.5 mm) each year. | Huaxing BI Bin LIU Jie WU Lei YUN Zhihan CHEN Zhewei CUI | 2009 | International Journal of Sediment Research2009，24，3： | 21 |
| 15 | The ChinaMAP analytics of deep whole genome sequences in 10,588 individuals显示文摘Metabolic diseases are the most common and rapidly growing health issues worldwide.The massive population-based human genetics is crucial for the precise prevention and intervention of metabolic disorders.The China Metabolic Analytics Project(ChinaMAP)is based on cohort studies across diverse regions and ethnic groups with metabolic phenotypic data in China.Here,we describe the centralized analysis of the deep whole genome sequencing data and the genetic bases of metabolic traits in 10,588 individuals from the ChinaMAP.The frequency spectrum of variants,population structure,pathogenic variants and novel genomic characteristics were analyzed.The individual genetic evaluations of Mendelian diseases,nutrition and drug metabolism,and traits of blood glucose and BMI were integrated.Our study establishes a large-scale and deep resource for the genetics of East Asians and provides opportunities for novel genetic discoveries of metabolic characteristics and disorders. | Yanan Cao Lin Li Min Xu Zhimin Feng Xiaohui Sun Jieli Lu Yu Xu Peina Du Tiange Wang Ruying Hu Zhen Ye Lixin Shi Xulei Tang Li Yan Zhengnan Gao Gang Chen Yinfei Zhang Lulu Chen Guang Ning Yufang Bi Weiqing Wang The ChinaMAP Consortium | 2020 | Cell Research2020，30，9： | 20 |
| 16 | A PP2C-1 Allele Underlying a Quantitative Trait Locus Enhances Soybean l O0-Seed Weight显示文摘栽培大豆可以在驯服期间失去某有用基因 loci。从野大豆的基因的基因渗入能拓宽基因背景并且改进大豆农学的特点。在这研究,通过 recombinant 的整个染色体的定序,生来的线人口源于在野大豆 ZYD7 和栽培大豆 HN44 之间的一个十字,并且为种子重量量的特点 loci 印射,我们发现从野大豆 ZYD7 的磷酸酶 2C-1 ( PP2C-1 )等位基因在种子重量/尺寸贡献增加。PP2C-1 可以由提高覆盖物的房间尺寸并且激活一个子集完成这功能播种特点相关的基因。我们发现 PP2C-1 与 GmBZR1 被联系, Arabidopsis BZR1 的大豆 ortholog,之一在发信号的 brassinosteroid (BR ) 给抄写因素调音,并且便于 dephosphorylated GmBZR1 的累积。相反,有在 N 终点的一些氨基酸的变化的 PP2C-2 等位基因没展出这功能。而且,我们证明 GmBZR1 能在转基因的植物支持种子重量 / 尺寸。通过栽培大豆就职的分析,我们发现 40% 检验就职没有 PP2C-1 等位基因,建议这些就职能被这等位基因的介绍改进。一起拿,我们的学习识别精英等位基因 PP2C-1,它能在由分子帮助繁殖的这等位基因的那操作可以增加的大豆,和针尖提高种子重量或尺寸在大豆的生产和另外的荚 / 庄稼。 | Xiang Lu Qing Xiong Tong Cheng Qing-Tian Li Xin-Lei Liu Ying-Dong Bi Wei Li Wan-Ke Zhang Biao Ma Yong-Cai Lai Wei-Guang Du Wei-Qun Man Shou-Yi Chen Jin-Song Zhang | 2017 | Molecular Plant2017，10，5： | 17 |
| 17 | Electroacupuncture preconditioning protects against focal cerebral ischemia/reperfusion injury via suppression of dynamin-related protein 1显示文摘Electroacupuncture preconditioning at acupoint Baihui(GV20) can reduce focal cerebral ischemia/reperfusion injury. However, the precise protective mechanism remains unknown. Mitochondrial fission mediated by dynamin-related protein 1(Drp1) can trigger neuronal apoptosis following cerebral ischemia/reperfusion injury. Herein, we examined the hypothesis that electroacupuncture pretreatment can regulate Drp1, and thus inhibit mitochondrial fission to provide cerebral protection. Rat models of focal cerebral ischemia/reperfusion injury were established by middle cerebral artery occlusion at 24 hours after 5 consecutive days of preconditioning with electroacupuncture at GV20(depth 2 mm, intensity 1 m A, frequency 2/15 Hz, for 30 minutes, once a day). Neurological function was assessed using the Longa neurological deficit score. Pathological changes in the ischemic penumbra on the injury side were assessed by hematoxylin-eosin staining. Cellular apoptosis in the ischemic penumbra on the injury side was assessed by terminal deoxyribonucleotidyl transferase-mediated d UTP-digoxigenin nick end labeling staining. Mitochondrial ultrastructure in the ischemic penumbra on the injury side was assessed by transmission electron microscopy. Drp1 and cytochrome c expression in the ischemic penumbra on the injury side were assessed by western blot assay. Results showed that electroacupuncture preconditioning decreased expression of total and mitochondrial Drp1, decreased expression of total and cytosolic cytochrome c, maintained mitochondrial morphology and reduced the proportion of apoptotic cells in the ischemic penumbra on the injury side, with associated improvements in neurological function. These data suggest that electroacupuncture preconditioning-induced neuronal protection involves inhibition of the expression and translocation of Drp1. | Gao-feng Zhang Pei Yang Zeng Yin Huai-long Chen Fu-guo Ma Bin Wang Li-xin Sun Yan-lin Bi Fei Shi Ming-shan Wang | 2018 | Neural Regeneration Research2018，13，1： | 16 |
| 18 | Ethnopharmacology, Phytochemistry, Pharmacology, Toxicology and Clinical Applications of Radix Astragali显示文摘Radix Astragali(RA),a traditional Chinese medicine from the dried root of Astragalus species,is widely distributed throughout the temperate regions of the world.The major bioactive constituents of RA are triterpene glycosides,flavonoids,saponins,and alkaloids,and these compounds mostly exert pharmacological activities on the cardiovascular,immune,respiratory,and hepatic systems.This review summarizes the recent studies on RA and provides a comprehensive summary regarding the status of resources,ethnopharmacology,phytochemistry,pharmacology,toxicology,clinical application,and patent release of RA.We hope this review can provide a guidance for further development of therapeutic agents from RA. | ZHANG Chun-hong YANG Xiao WEI Jing-ran CHEN Na-mu-han XU Jian-ping BI Ya-qiong YANG Min GONG Xue LI Zi-yan REN Kai HAN Qi-heng ZHANG Lei LI Xue JI Ming-yue WANG Cong-cong LI Min-hui | 2021 | Chinese Journal of Integrative Medicine2021，27，3： | 16 |
| 19 | Inhibition of Autophagy by Estradiol Promotes Locomotor Recovery after Spinal Cord Injury in Rats显示文摘17β-estradiol(E2) has been shown to have neuroprotective effects in different central nervous system diseases. The mechanisms underlying estrogen neuroprotection in spinal cord injury(SCI) remain unclear. Previous studies have shown that autophagy plays a crucial role in the course of nerve injury. In this study, we showed that E2 treatment improved the restoration of locomotor function and decreased the loss of motor neurons in SCI rats. Realtime PCR and western blot analysis revealed that the protective function of E2 was related to the suppression of LC3 II and beclin-1 expression. Immunohistochemical study further confirmed that the immunoreactivity of LC3 in the motor neurons was down-regulated when treated with E2. In vitro studies demonstrated similar results that E2 pretreatment decreased the autophagic activity induced by rapamycin(autophagy sensitizer) and increased viability in a PC12 cell model. These results indicated that the neuroprotective effects of E2 in SCI are partly related to the suppression of excessive autophagy. | Chao-Wei Lin Bi Chen Ke-Lun Huang Yu-Sen Dai Hong-Lin Teng | 2016 | Neuroscience Bulletin2016，32，2： | 14 |
| 20 | Assessment on environmental quality of heavy metals in agricultural soils of Chongming Island, Shanghai City显示文摘重金属的环境质量( Pb , Cd , Cr ,同样 Hg )在 Chongming 的农业表面土壤,岛被国家、本地、职业的标准基于大规模调查估计在哪个从蔬菜的 28 件样品阴谋,来自稻地的 65 件样品和来自西瓜地的 9 件样品从整个岛区域被收集。结果证明 Pb 的平均集中, Cd, Cr,并且 Hg 是 21.6 mg ???? | SUN Chao BI Chunjuan CHEN Zhenlou WANG Dongqi ZHANG Cui SUN Yuedi YU Zhongjie ZHOU Dong | 2010 | Journal of Geographical Sciences2010，20，1： | 14 |
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