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| 1 | Identification of IFN-γ-producing innate B cells显示文摘 | Yan Bao Xingguang Liu Chaofeng Han Sheng Xu Bin Xie Qian Zhang Yan Gu Jin Hou Li Qian Cheng Qian Huanxing Han Xuetao Cao | 2014 | Cell Research2014，24，2： | 3 |
| 2 | Induction of Siglec-G by RNA Viruses Inhibits the Innate Immune Response by Promoting RIG-I Degradation显示文摘 | Weilin Chen Chaofeng Han Bin Xie Xiang Hu Qian Yu Liyun Shi Qingqing Wang Dongling Li Jianli Wang Pan Zheng Yang Liu Xuetao Cao | 2013 | Cell2013，，3： | 2 |
| 3 | Construction of recombinant plasmid pEGFP-C2-L539fs/47 and its expression in HEK293 cells显示文摘Objective:To reconstruct pEGFP-C2-L539fs/47,a HERG nonsense mutant in eukaryotic expression plasmid,and observe the fusion protein expressed in HEK293 cells(human embryo kidney cells).Methods:After double digestion of pcDNA3-L539fs/47 and pEGFP-C2-HERG with sbf I and Eco91 I,the small product fragment,from pcDNA3-L539fs/47,was subcloned into the big fragment of pEGFP-C2-HERG under T4 ligase.pEGFP-C2-L539fs/47 was identified by agarose gel electrophoresis and sequencing.pcDNA3-L539fs/47 and pEGFP-C2-L539fs/47 were transiently transfected into HEK293 cells by Lipofect,respectively.The expression of fusion protein in HEK293 cells was detected through immunofluorescence,laser confocal imaging scanning in vivo,Western blot and PCR.Results:Mutation region cDNA fragment(about 1 kb) and target vector fragment(about 7.2 kb) were ligated after purification and gel recovery.Agarose gel electrophoresis and sequencing successfully demonstrated eukaryotic expression plasmid pEGFP-C2-L539fs/47,constructed approximately 8.2 kb,sequencing consistent with template gene.The transfection efficiency of recombinant plasmid by fluorescence microscopy was more than60%.Western blot analysis detected pcDNA3-L539fs/47 expression of the protein size 60 KD,the expression of pEGFP-C2 fusion protein size of approximately 90 KD.The L539fs/47 gene expression in HEK293 cells was significant by PCR analysis.Confocal laser imaging showed that pEGFP-C2-L539fs/47 protein was successfully expressed in cytoplasm and cytomembrane of HEK293 cells.Conclusion:pEGFP-C2-L539fs/47 containing the HERG gene mutant was successfully constructed by double digestion method and expressed fusion protein in HEK293 cells,which laid a foundation for the further study on L539fs/47. | Lue Ying Zhang Aifeng Han Wenqi Li Guoliang Zhang Junbo Gao Jie Pan Junqiang Zhang Yong Sun Chaofeng | 2012 | Journal of Medical Colleges of PLA(China)2012，27，3： | 2 |
| 4 | Molecular Characterisation of the Faecal Microbiota in Patients with Type II Diabetes显示文摘 | Xiaokang Wu Chaofeng Ma Lei Han Muhammad Nawaz Fei Gao Xuyan Zhang Pengbo Yu Chang’an Zhao Lianchuan Li Aiping Zhou Juan Wang John E. Moore B. Cherie Millar Jiru Xu | 2010 | Current Microbiology2010，，1： | 2 |
| 5 | Nested PCR-denaturing gradient gel electrophoresis analysis of human skin microbial diversity with age显示文摘 | Wei Li Lei Han Pengbo Yu Chaofeng Ma Xiaokang Wu Jiru Xu | 2014 | Microbiological Research2014，，： | 1 |
| 6 | The methyltransferase PRMT6 attenuates antiviral innate immunity by blocking TBK1–IRF3 signaling显示文摘Protein arginine methyltransferases(PRMTs)play diverse biological roles and are specifically involved in immune cell development and inflammation.However,their role in antiviral innate immunity has not been elucidated.Viral infection triggers the TBK1–IRF3 signaling pathway to stimulate the production of type-I interferon,which mediates antiviral immunity.We performed a functional screen of the nine mammalian PRMTs for regulators of IFN-βexpression and found that PRMT6 inhibits the antiviral innate immune response.Viral infection also upregulated PRMT6 protein levels.We generated PRMT6-deficient mice and found that they exhibited enhanced antiviral innate immunity.PRMT6 deficiency promoted the TBK1–IRF3 interaction and subsequently enhanced IRF3 activation and type-I interferon production.Mechanistically,viral infection enhanced the binding of PRMT6 to IRF3 and inhibited the interaction between IRF3 and TBK1;this mechanism was independent of PRMT6 methyltransferase activity.Thus,PRMT6 inhibits antiviral innate immunity by sequestering IRF3,thereby blocking TBK1-IRF3 signaling.Our work demonstrates a methyltransferase-independent role for PRMTs.It also identifies a negative regulator of the antiviral immune response,which may protect the host from the damaging effects of an overactive immune system and/or be exploited by viruses to escape immune detection. | Hua Zhang Chaofeng Han Tianliang Li Nan Li Xuetao Cao | 2019 | Cellular & Molecular Immunology2019，16，10： | 1 |
| 7 | Induction of Siglec-G by RNA Viruses Inhibits the Innate Immune Response by Promoting RIG-I Degradation显示文摘 | Weilin Chen Chaofeng Han Bin Xie Xiang Hu Qian Yu Liyun Shi Qingqing Wang Dongling Li Jianli Wang Pan Zheng Yang Liu Xuetao Cao | 2013 | Cell2013，，3： | 1 |
| 8 | Heat Shock Protein 70, Released from Heat Stressed Tumor Cells, Initiates Antitumor Immunity by Inducing Tumor Cell Chemokine Production and Ae tivating Dendritic Cells via TLR4 Pathway显示文摘 | Taoyong Chen Jun Guo Chaofeng Han | 2009 | The Journal of Im munology2009，182，： | 1 |
| 9 | Molecular Characteri- sation of the Faecal Microbiota in Patients with Type II Diabetes 显示文摘 | A Xiaokang W Chaofeng M Lei Han et al | 2010 | Curt Microbiol2010，61，： | 1 |
| 10 | Preparation of carbon nitride nanoparticles by nanoprecipitation method with high yield and enhanced photocatalytic activity显示文摘As an emerging 2D conjugated material,graphitic carbon nitride(CN) has attracted great research attention as important catalytic medium for transforming solar energy.Nanostructure modulation of CN is an effective way to improve catalytic activities and has been extensively investigated,but remains challenging due to complex processes,time consuming or low yield.Here,taking advantage of recent discovered good solvents for CN,a nanoprecipitation approach using poor solvents is proposed for preparation of CN nanoparticles(CN NPs).With simple processes of CN dissolution and precipitation,we can quickly synthesize CN NPs(^40 nm) with a yield of up to 50%,the highest one to the best of our knowledge.As an example of potential applications,the as-prepared CN NPs were applied to photocatalytic degradation of dyes with an evident boosted performance up to 2.5 times.This work would open a new way for batch preparation of nanostructured CN and pave its large-scale industrial applications. | Ziyu Gan Chaofeng Huang Yanfei Shen Qing Zhou Dan Han Jin Ma Songqin Liu Yuanjian Zhang | 2020 | Chinese Chemical Letters2020，31，2： | 1 |
| 11 | Outbreak of acute respiratory disease caused by human adenovirus type 7 in a military training camp in Shaanxi, China显示文摘 | Pengbo Yu Chaofeng Ma Muhammad Nawaz Lei Han Jianfang Zhang Quanli Du Lixia Zhang Qunling Feng Jingjun Wang Jiru Xu | 2013 | Microbiol Immunol2013，，8： | 1 |
| 12 | Construction of a HERG mutant L539fs/47-*558W pEGFP vector and the expression of the fusion protein in HEK293 cells显示文摘Objective:To construct a human ether-a-go-go-related gene(HERG)nonsense mutant L539fs/47-*558W into the autonomously fluorescent,eukaryotic expression vector pEGFP-C2,and to verify expression of the reconstruct in human embryonic kidney-293(HEK293)cells.Methods:The mutational fragment was subcloned into pEGFP-C2-HERG by double digestion of SbfⅠ,Eco91Ⅰand rejoining of T4 ligase.After verification,the recombinant pEGFP-C2-L539fs/47-*558W and pEGFP-C2-HERG were respectively transfected into HEK293 cells for 48 h by the Lipofect method to observe the expression location of the fusion protein by laser confocal imaging scanning in vivo.pcDNA3-L539fs/47-*558W and pcDNA3-HERG were transfected to observe the expression location of the HERG protein by immunofluoresceoce.The mutant protein size was determined by Western blotting.Results:The about 1 kb-sized mutation region cDNA fragment from pcDNA3-L539fs/47-*558W and the about 7.2 kb-sized target vector fragment from pcDNA3-HERG were ligated after purification and gel recovery.pEGFP-C2-L539fs/47*-558W,approximately 8.2 kb,was demonstrated successfully been constructed under agarose gel electrophoresis and further sequencing.Laser confocal imaging showed that pEGFP-C2-HERG was mainly expressed in the membrane,whereas truncated mutant-type HERG in the pEGFP-C2 vector was partially located in the cytoplasm,the others were transported to the cell membrane in living HEK293 cells.The same as the immunofluoresceoce results after transfection of pcDNA3-HERG and pcDNA3-L539fs/47-558W.Wild-type HERG-GFP fusion protein expressed 160 and 180 kDa bands.The mutant and mutant-GFP fusion proteins were 70 and 100 kDa,respectively.Conclusion:pEGFP-C2-L539fs/47-*558W was successfully constructed by double digestion method GFP had no effect on its protein expression and trafficking in HEK293 cells,which laid a foundation for the further study on L539fs/47-*558W. | ZHANG Junbo Lü Ying ZHANG Aifeng SUN Chaofeng HAN Wenqi LI Guoliang GAO Jie HUO Jianhua PAN Junqiang ZHOU Xin NIU Xiaolin | 2013 | Journal of Medical Colleges of PLA(China)2013，28，4： | 0 |
| 13 | Doctors' faith显示文摘We make some efforts to find some connections between religion and medical science. Medical science can be regarded as sacred and holy as a religion for doctors to believe. The common points between them are the basis to successfully integrate them into doctors' faith. Furthermore, we explain that doctors need a faith to believe so as to meet the demand of humanitarian traits we should have and to do better in the scientific work and win in the battles with the diseases. | ZHANG Junbo Lü Ying ZHANG Aifeng SUN Chaofeng HAN Wenqi LI Guoliang GAO Jie HUO Jianhua PAN Junqiang ZHOU Xin NIU Xiaolin | 2013 | Journal of Medical Colleges of PLA(China)2013，28，1： | 0 |
| 14 | Correction to: The methyltransferase PRMT6 attenuates antiviral innate immunity by blocking TBK1-IRF3 signaling显示文摘Correction to:Cellular&Molecular Immunology(2019)16,800–809;http://gffzzd3cc09b8251d45dfsovuck05ckuov6v9k.ffgz.tsg.suse.edu.cn/10.1038/s41423-018-0057-4,published online 4 July 2018 In the published version of Fig.2d,the HE image for Prmt6+/+mice of the medium group was mistakenly presented.Figure 2d has now been corrected.The corrected version of Figure 2 is shown below. | Hua Zhang Chaofeng Han Tianliang Li Nan Li Xuetao Cao | 2020 | Cellular & Molecular Immunology2020，17，2： | 0 |
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