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| 1 | The Reference Genome of Tea Plant and Resequencing of 81 Diverse Accessions Provide Insights into Its Genome Evolution and Adaptation显示文摘Tea plant is an important economic crop,which is used to produce the world's oldest and most widely consumed tea beverages.Here,we present a high-quality reference genome assembly of the tea plant(Camellia sinensis var.sinensis)consisting of 15 pseudo-chromosomes.LTR retrotransposons(LTR-RTs)account for 70.38%of the genome,and we present evidence that LTR-RTS play critical roles in genome size expansion and the transcriptional diversification of tea plant genes through preferential insertion in promoter regions and introns.Genes,particularly those coding for terpene biosynthesis pro-teins,associated with tea aroma and stress resistance were significantly amplified through recent tandem duplications and exist as gene clusters in tea plant genome.Phylogenetic analysis of the sequences of 81 tea plant accessions with diverse origins revealed three well-differentiated tea plant populations,support-ing the proposition for the southwest origin of the Chinese cultivated tea plant and its later spread to western Asia through introduction.Domestication and modern breeding left significant signatures on hundreds of genes in the tea plant genome,particularly those associated with tea quality and stress resis-tance.The genomic sequences of the reported reference and resequenced tea plant accessions provide valuable resources for future functional genomics study and molecular breeding of improved cul-tivars of tea plants. | Enhua Xia Wei Tong Yan Hou Yanlin An Linbo Chen Qiong Wu Yunlong Liu Jie Yu Fangdong Li Ruopei Li Penghui Li Huijuan Zhao Ruoheng Ge Jin Huang Ali Inayat Mallano Yanrui Zhang Shengrui Liu Weiwei Deng Chuankui Song Zhaoliang Zhang Jian Zhao Shu Wei Zhengzhu Zhang Tao Xia Chaoling Wei Xiaochun Wan | 2020 | Molecular Plant2020，13，7： | 51 |
| 2 | Spatio-temporal variations of the bacterioplankton community composition in Chaohu Lake, China显示文摘Despite considerable attention paid to Chaohu Lake in China, the dynamics of bacterioplankton community composition (BCC) on spatial and seasonal scales are poorly understood.In this study, water samples were collected from autumn 2006 to summer 2007 at five positions in Chaohu Lake with difierent trophic status.BCC of these samples was determined by both the PCR amplification of the 16S rDNA gene and the denaturing gradient gel electrophoresis (DGGE).The abundance and diversity of bacterioplankton communities at difierent sampling positions showed similar seasonal patterns.The BCCs in the samples varied substantially, and the pattern of changes indicated that the seasonal difierence might have a significant impact on the BCC's structure in the lake.Canonical correspondence anal-ysis (CCA) on the DGGE patterns and physicochemical parameters indicated that the temperature and the levels of 5-d biochemical O2 demand (BOD5), NH3-N, CODMn, total nitrogen, total phosphorous, and dissolved oxygen significantly infiuenced the BCC, and four of the seven variables were related to the level of eutrophication.Our results indicate that eutrophic status and season are the most infiu-ential factors in determining BCC in Chaohu Lake. | Chaoling Wei Sumin Bao Xinyan Zhu Xiangming Huang | 2008 | Progress in Natural Science:Materials International2008，18，9： | 9 |
| 3 | A Data Assured Deletion Scheme in Cloud Storage显示文摘In order to provide a practicable solution to data confidentiality in cloud storage service,a data assured deletion scheme,which achieves the fine grained access control,hopping and sniffing attacks resistance,data dynamics and deduplication,is proposed.In our scheme,data blocks are encrypted by a two-level encryption approach,in which the control keys are generated from a key derivation tree,encrypted by an All-OrNothing algorithm and then distributed into DHT network after being partitioned by secret sharing.This guarantees that only authorized users can recover the control keys and then decrypt the outsourced data in an ownerspecified data lifetime.Besides confidentiality,data dynamics and deduplication are also achieved separately by adjustment of key derivation tree and convergent encryption.The analysis and experimental results show that our scheme can satisfy its security goal and perform the assured deletion with low cost. | LI Chaoling CHEN Yue ZHOU Yanzhou | 2014 | China Communications2014，11，4： | 7 |
| 4 | Long-term anodal block stimulation at sacral anterior roots promoted recovery of neurogenic bladder function in a rabbit model of complete spinal cord injury显示文摘A complete spinal cord injury model was established in experimental rabbits using the spinal cord clip compression method.Urodynamic examination was performed 2 weeks later to determine neurogenic bladder status.The rabbits were treated with anodal block stimulation at sacral anterior roots for 4 weeks.Electrical stimulation of sacral anterior roots improved urodynamic parameters of neurogenic bladder in rabbit models of complete spinal cord injury,effectively promoted urinary function,and relieved urinary retention.Immunohistochemistry results showed that a balance was achieved among expression of muscarinic receptor subunits M2,M3,ATP-gated ion channel P2X3 receptors,and β2-adrenergic receptor,and nerve growth factor expression decreased.These results suggested that long-term sacral anterior root stimulation of anodal block could be used to treat neurogenic bladder in a rabbit model of complete spinal cord injury. | Xiaoran Wang Qi Gao Xiaoyu Yang Weihua Wang Xinquan Gu Guifeng Liu Peng Yan Ge Gao Xin Yu Yongjie Wang Jihu Lian Chaoling Shi Yao Wang Li Fan | 2012 | Neural Regeneration Research2012，7，5： | 6 |
| 5 | Electronic structure, chemical bond and thermal stability of hydrogen absorber Li_2MgN_2H_2显示文摘The lowest total energy crystal structure of Li2MgN2H2 was identified by the first principle calculation with RPBE exchange-correlation function. Furthermore, the fine structure parameters of this crystal structure were calculated with PBE and PW91 exchange-correlation function. In a further step, the density of states, electron density, charge density difference on (0 0 1) plane and heat of formation of Li2MgN2H2 hydrogenation were computed with PW91 exchange-correlation function. Consequently, the electronic structures and chemical bonds in this compound were analyzed and discussed, and then the thermodynamic character of Li2MgN2H2 for hydrogen storage was evacuated. | WANG Qiang CHEN YunGui WU ChaoLing TAO MingDa GAI JingGang | 2009 | Chinese Science Bulletin2009，54，3： | 2 |
| 6 | Eff ect of polystyrene microplastics and temperature on growth,intestinal histology and immune responses of brine shrimp Artemia franciscana显示文摘Microplastics pollution and seawater temperature rise have been the major environmental issues,threatening the survival and biodiversity of marine organisms.This study evaluated the combined effect of temperature and polystyrene microplastics(MP)on Artemia,a filter-feeding crustacean that is widely used for environmental toxicology studies.Brine shrimp Artemia franciscana were exposed to three MP concentrations(0,0.2,and 2.0 mg/L)and three temperatures(22,26,and 30℃)for 14 d.In general,higher MP concentration and temperature led to a decreased survival rate and growth.Two-way ANOVA analysis indicated that the survival rate of Artemia was significantly impacted by both MP concentration and temperature(P<0.05),but there was no significant interaction between two factors(P>0.05).Growth of Artemia was significantly impacted by temperature(P<0.05),and with a significant interaction between two factors(P<0.05).Furthermore,the enzymatic activity,intestinal histological analyses,and immune gene expression were determined for Artemia reared at 30℃ with three MP concentrations(0,0.2,and 2.0 mg/L).The results showed that 2.0-mg/L MP resulted in reduced Artemia intestinal microvilli and exfoliated epithelia cells,significantly increased acid phosphatase(ACP)activity(P<0.05)and immunerelated gene ADRA1 B and CREB3 expression,revealing that higher MP concentration could induce oxidative and immunological stress on Artemia at 30℃.Overall,our study suggests that MP and temperature have combined adverse effect on Artemia,especially at relatively high temperature and polystyrene MP concentration.These findings are important to understand the potential ecological risks posed by these two factors on the organisms in marine environment. | Xuekai HAN Yuyu ZHENG Chaoling DAI Hu DUAN Meirong GAO Md Rayhan ALI Liying SUI | 2021 | Journal of Oceanology and Limnology2021，39，3： | 2 |
| 7 | Improved image resolution on thoracic carcinomas by quantitative 18F-FDG coincidence SPECT/CT in comparison to 18F-FDG PET/CT显示文摘Currently,18F-FDG coincidence SPECT(Co-SPECT)/CT scan still serves as an important tool for diagnosis,staging,and evaluation of cancer treatment in developing countries.We implemented full physical corrections(FPC) to Co-SPECT(quantitative Co-SPECT) to improve the image resolution and contrast along with the capability for image quantitation.FPC included attenuation,scatter,resolution recovery,and noise reduction.A standard NEMA phantom filled with 10:1 F-18 activity concentration ratio in spheres and background was utilized to evaluate image performance.Subsequently,15 patients with histologically confirmed thoracic carcinomas were included to undergo a 18 F-FDG Co-SPECT/CT scan followed by a 18 F-FDG PET/CT scan.Functional parameters as SUVmax,SUVmean,SULpeak,and MTV from both quantitative Co-SPECT and PET were analyzed.Image resolution of Co-SPECT for NEMA phantom was improved to reveal the smallest sphere from a diameter of 28 mm to 22 mm(17 mm for PET).The image contrast was enhanced from 1.7 to 6.32(6.69 for PET) with slightly degraded uniformity in background(3.1% vs.6.7%)(5.6% for PET).Patients’ SUVmax,SUVmean,SULpeak,and MTV measured from quantitative Co-SPECT were overall highly correlated with those from PET(r=0.82-0.88).Adjustment of the threshold of SUVmax and SUV to determine SUVmean and MTV did not further change the correlations with PET(r=0.81-0.88).Adding full physical corrections to Co-SPECT images can significantly improve image resolution and contrast to reveal smaller tumor lesions along with the capability to quantify functional parameters like PET/CT. | Yuming Zheng Chaoling Jin Huijuan Cui Haojie Dai Jue Yan Pingping Han Bailing Hsu | 2020 | The Journal of Biomedical Research2020，34，4： | 2 |
| 8 | Duplication and transcriptional divergence of three Kunitz protease inhibitor genes that modulate insect and pathogen defenses in tea plant(Camellia sinensis)显示文摘Kunitz protease inhibitors(KPIs)are ubiquitous in plants and act as crucial compounds in defense responses against insect attack and pathogen infection.However,the influence of gene duplication on the postdivergence of the CsKPI genes involved in biotic stresses in tea plant is not well known.Here,we identified three CsKPI genes from tea plant(Camellia sinensis)and characterized their expression and evolutionary patterns among plant species.We found that CsKPI1,CsKPI2,and CsKPI3 diverged from their common ancestor 72.94 million years ago(MYA),and the tandem duplication of CsKPI2 and CsKPI3 occurred 26.78 MYA.An in vitro protein assay showed that the three CsKPI proteins were functional and inhibited the production of p-nitroanilide(PNA)from an artificial substrate.The three CsKPI-GFP fusion proteins localized to the cytoplasm.We showed that salicylic acid(SA)and transcripts of CsKPI2 and CsKPI3 significantly accumulated after infection with Glomerella cingulata.The application of exogenous SA stimulated the high expression of both CsKPI2 and CsKPI3 by activating cis-elements within their promoters.Under Ectropis oblique attack,CsKPI1 expression and jasmonic acid(JA)levels were more abundant in both insect-damaged leaf tissues and undamaged neighboring leaves.The application of jasmonic acid methyl ester elicited high expression levels of CsKPI1,suggesting that CsKPI1 accumulation requires JA production in tea plant.The overall findings suggest that the transcriptional divergence of KPI genes after duplication led to the specialized role of CsKPI1 in the physiological response to insect stress;the functional conservation between CsKPI2 and CsKPI3 confers resistance to pathogen infection in tea plant. | Junyan Zhu Yaxian He Xiaomei Yan Lu Liu Rui Guo Xiaobo Xia Daojie Cheng Xiaozeng Mi Lidiia Samarina Shenrui Liu Enhua Xia Chaoling Wei | 2019 | Horticulture Research2019，6，1： | 2 |
| 9 | The structure and high-temperature (333K) electrochemical performance of La 0.8? x Ce x Mg 0.2 Ni 3.5 ( x =0.00–0.20) hydrogen storage alloys显示文摘 | Shen Xiangqian Chen Yungui Tao Mingda Wu Chaoling Deng Gang Kang Zhenzhen | 2009 | International Journal of Hydrogen Energy2009，，8： | 1 |
| 10 | Effect of Al on hydrogen storage properties of V 30 Ti 35 Cr 25 Fe 10 alloy显示文摘 | Yigang Yan Yungui Chen Hao Liang Chaoling Wu Mingda Tao Tu Mingjing | 2006 | Journal of Alloys and Compounds2006，，1： | 1 |
| 11 | Catalytic effect and reaction mechanism of Ti doped in NaAlH_4: A review显示文摘Catalytic effect and hydrogen reaction mechanism of Ti doped in NaAlH4 were elaborated in this paper, and current viewpoints about Ti active species in hydrogen reaction were discussed. In a further step, the possibility and practicality of the hydrogen reaction mechanism of Ti-doped NaAlH_4 were elucidated. They could be summarized as follows: while the current theory about the hydrogen reaction mecha-nism of Ti-doped NaAlH_4 should be further improved and modified, the research on Ti-doped NaAlH_4 would be a recommendable pattern for the catalyst research in other metal complex hydrides. | WANG Qiang CHEN YunGui WU ChaoLing TAO MingDa | 2008 | Chinese Science Bulletin2008，53，12： | 1 |
| 12 | Low-temperature hydrogen storage alloy and its application in NiMH battery显示文摘 | Tao Mingda Chen Yungui Wu Chaoling | 2004 | J Rare Earths2004，22，6： | 1 |
| 13 | Kallikrein gene deliver attenuates renal function in goldblatt hypertensive rats显示文摘 | WangC ChaoL | 1998 | Hypertension1998，31，： | 1 |
| 14 | Study of the synthesis,crystallization,and morphology of poly(ethylene glycol) -poly(ε-caprutactone) dibloek copolymers显示文摘 | Sun Jingru Dang Chao | 2004 | Biomacromolecules2004，5，5： | 1 |
| 15 | Diagnosis and management of primary aldosteronism:An updated review显示文摘 | Chia-Ter Chaol Vin-Cent Wul Chin-Chi Kuo | 2013 | Ann Edicine2013，45，： | 1 |
| 16 | A new ATP-sensitive potassium channel opener reduces blood pressure and reversed cardiovascular remodeling in experimental hypertension显示文摘 | Wang H Chaoling L Zhang Y | 2005 | J Pharmacol Exp Ther2005，312，3： | 1 |
| 17 | Light-induced expression of genes involved in phenylpropanoid biosynthetic pathways in callus of tea ( Camellia sinensis (L.) O. Kuntze)显示文摘 | YunSheng Wang LiPing Gao ZhengRong Wang YaJun Liu MeiLian Sun DongQing Yang ChaoLing Wei Yu Shan Tao Xia | 2011 | Scientia Horticulturae2011，，： | 1 |
| 18 | Towards Comprehensive Provable Data Possession in Cloud Computing显示文摘To check the remote data integrity in cloud computing,we have proposed an efficient and full data dynamic provable data possession(PDP) scheme that uses a SN(serial number)-BN(block number) table to support data block update.In this article,we first analyze and test its performance in detail.The result shows that our scheme is efficient with low computation,storage,and communication costs.Then,we discuss how to extend the dynamic scheme to support other features,including public auditability,privacy preservation,fairness,and multiple-replica checking.After being extended,a comprehensive PDP scheme that has high efficiency and satisfies all main requirements is provided. | LI Chaoling CHEN Yue TAN Pengxu YANG Gang | 2013 | Wuhan University Journal of Natural Sciences2013，18，3： | 1 |
| 19 | Mechanism of gentamicin nephrotoxicity in rats and the protective effect of zinc-induced metallothionein synthesis显示文摘 | XUEHAI D U CHAOLING Y | 1994 | Nephrol Dial Transplant1994，9，： | 1 |
| 20 | Microstructure of Ba_(0.615)Sr_(0.35)Mg_(0.035)TiO_3 dielectric ceramics via X-ray spectrum analysis显示文摘The compound Ba0.615Sr0.35Mg0.035TiO3 was analyzed by the X-ray dispersed spectroscopy analyzer (SEM-EDAX) with the spec-trum image method, a powerful tool for chemical phase identification. The method was first time applied on a ceramic material to collect spectrum image and was progressed to identify the Mg-enriched component with fine scale pocket shown in matrix. This result, together with the stoichiometry result obtained from spot mode spectrum analysis, strongly confirmed the microstructure and properties of dielectric materials. | QI Wen XUE Tao LI Xuan FANG DongYu LI ChaoLing YAO Pei | 2012 | Chinese Science Bulletin2012，57，34： | 1 |
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