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| 1 | Telomerase activity in colorectal cancer,prognostic factor and implications in the microsatellite instability pathway显示文摘AIM: To determine whether the telomerase activity is related to the Microsatellite instability (MSI) genetic pathway and whether it means a difference in the survival. METHODS: The population consisted of 97 colorectal cancer patients. MSI determination was performed in accordance with the NCI criteria using PCR and Genescan. Telomerase activity was determined by the TRAP-assay, an ELISA procedure based on the amplification of telomeric repeat sequences. RESULTS: 6.2% showed high MSI (MSI-H), 10.3% showed low MSI (MSI-L) and 83.5% did not show this alteration (MSS). Positive telomerase activity was detected in 92.8% of the patients. 83.3% of MSI-H tumors showed positive telomerase against 93.8% of MSS tumors. In the overall survival analysis the absence of telomerase activity conferred a better prognosis. CONCLUSION: Previous works have shown that tumors which develop via the MSI pathway present a better prognosis. No link between telomerase activity and MSI status is observed, although sample sizes are small. Patients with telomerase negative tumors had better overall survival than patients with telomerase positive tumors. | M Vidaurreta ML Maestro S Rafael S Veganzones MT Sanz-Casla J Cerdán M Arroyo | 2007 | World Journal of Gastroenterology2007，13，28： | 8 |
| 2 | Effects of length and application rate of rice straw mulch on surface runoff and soil loss under laboratory simulated rainfall显示文摘Forest land affected by deforestation yields high soil and water losses.Suitable management practices need to be found that can reduce these losses and achieve ecological and hydrological sustainability of the deforested areas.Mulch has been found to be effective in reducing soil losses;straw mulch is easy to apply,contributes soil organic matter,and is efficient since the day of application.However,the complex effects of rice straw mulch with different application rates and lengths on surface runoff and soil loss have not been clarified in depth.The current paper evaluates the efficiency of rice straw mulch in reducing the hydrological response of a silty clay loam soil under high intensity and low frequency rainfall events(tap water with total depth of 49 mm and intensity of 98 mm/h)simulated in the laboratory.Surface runoff and soil loss at three lengths of the straw(10,30,and 200 mm)and three application rates(1,2,and 3 Mg/ha)were measured in 50 cm(width)×100 cm(length)×10 cm(depth)plots with disturbed soil samples(aggregate soil size<4 mm)collected in a deforested area.Bare soil was used as control experiment.Runoff volume and erosion were significantly(at p<0.05)lower in mulched soils compared to control plots.These reductions were ascribed to the water absorption capacity of the rice straw and the protection cover of the mulch layer.The minimum runoff was observed for a mulch layer of3 Mg/ha of straw with a length of 200 mm.The lowest soil losses were found with straw length of10 mm.The models developed predict runoff and erosion based on simple linear functions of mulch application rate and length,and can be used for a suitable hydrological management of soil.It is concluded that,thanks to rice straw mulch used as an organic soil conditioner,soil erosion and surface runoff are significantly(at p<0.05)reduced,and the mulch protection contributes to reduce the risk of soil degradation.Further research is,however,needed to analyze the upscaling of the hydrological effects of mulching from the plot to the hillslope scale. | Misagh Parhizkar Mahmood Shabanpour Manuel Esteban Lucas-Borja Demetrio Antonio Zema Siyue Li Nobuaki Tanaka Artemio Cerdà | 2021 | International Journal of Sediment Research2021，36，4： | 8 |
| 3 | Effect of soil management on soil erosion on sloping farmland during crop growth stages under a large-scale rainfall simulation experiment显示文摘Soil erosion on farmland is a critical environmental issue and the main source of sediment in the Yellow River, China. Thus, great efforts have been made to reduce runoff and soil loss by restoring vegetation on abandoned farmland. However, few studies have investigated runoff and soil loss from sloping farmland during crop growth season. The objective of this study was to investigate the effects of soil management on runoff and soil loss on sloping farmland during crop growth season. We tested different soybean growth stages (i.e., seedling stage (R1), initial blossoming stage (R2), full flowering stage (R3), pod bearing stage (R4), and initial filling stage (R5)) and soil management practice (one plot applied hoeing tillage (HT) before each rainfall event, whereas the other received no treatment (NH)) by applying simulated rainfall at an intensity of 80 mm/h. Results showed that runoff and soil loss both decreased and infiltration amount increased in successive soybean growth stages under both treatments. Compared with NH plot, there was less runoff and higher infiltration amount from HT plot. However, soil loss from HT plot was larger than that from NH plot in R1–R3, but lower in R4 and R5. In the early growth stages, hoeing tillage was effective for reducing runoff and enhancing rainfall infiltration. By contrast, hoeing tillage enhanced soil and water conservation during the late growth stages. The total soil loss from HT plot (509.0 g/m^2) was 11.1% higher than that from NH plot (457.9 g/m^2) in R1–R5. However, the infiltration amount from HT plot (313.9 mm) was 18.4% higher than that from NH plot (265.0 mm) and the total runoff volume from HT plot was 49.7% less than that from NH plot. These results indicated that crop vegetation can also act as a type of vegetation cover and play an important role on sloping farmland. Thus, adopting rational soil management in crop planting on sloping farmland can effectively reduce runoff and soil loss, as well as maximize rainwater infiltration during crop growth period. | WANG Linhua WANG Yafeng Keesstra SASKIA Cerdà ARTEMI MA Bo WU Faqi | 2018 | Journal of Arid Land2018，10，6： | 5 |
| 4 | Interplay between post-translational cyclooxygenase-2 modifications and the metabolic and proteomic profile in a colorectal cancer cohort显示文摘BACKGROUND Colorectal cancer(CRC) is the second most common cause of cancer death worldwide. It is broadly described that cyclooxygenase-2(COX-2) is mainly overexpressed in CRC but less is known regarding post-translational modifications of this enzyme that may regulate its activity, intracellular localization and stability. Since metabolic and proteomic profile analysis is essential for cancer prognosis and diagnosis, our hypothesis is that the analysis of correlations between these specific parameters and COX-2 state in tumors of a high number of CRC patients could be useful for the understanding of the basis of this cancer in humans.AIM To analyze COX-2 regulation in colorectal cancer and to perform a detailed analysis of their metabolic and proteomic profile.METHODS Biopsies from both healthy and pathological colorectal tissues were taken under informed consent from patients during standard colonoscopy procedure in the University Hospital of Bellvitge(Barcelona, Spain) and Germans Trias i Pujol University Hospital(Campus Can Ruti)(Barcelona, Spain). Western blot analysis was used to determine COX-2 levels. Deglycosylation assays were performed in both cells and tumor samples incubating each sample with peptide N-glycosidase F(PNGase F). Prostaglandin E2(PGE2) levels were determined using a specific ELISA. 1 H high resolution magic angle spinning(HRMAS) analysis was performed using a Bruker AVIII 500 MHz spectrometer and proteomic analysis was performed in a nano-liquid chromatography-tandem mass spectrometer(nano LC-MS/MS) using a QExactive HF orbitrap MS.RESULTS Our data show that COX-2 has a differential expression profile in tumor tissue of CRC patients vs the adjacent non-tumor area, which correspond to a glycosylated and less active state of the protein. This fact was associated to a lesser PGE2 production in tumors. These results were corroborated in vitro performing deglycosylation assays in HT29 cell line where COX-2 protein profile was modified after PNGase F incubation, showing higher PGE2 levels. Moreover,HRMAS analysis indicated that tumor tissue has altered metabolic features vs non-tumor counterparts, presenting increased levels of certain metabolites such as taurine and phosphocholine and lower levels of lactate. In proteomic experiments, we detected an enlarged number of proteins in tumors that are mainly implicated in basic biological functions like mitochondrial activity,DNA/RNA processing, vesicular trafficking, metabolism, cytoskeleton and splicing.CONCLUSION In our colorectal cancer cohort, tumor tissue presents a differential COX-2 expression pattern with lower enzymatic activity that can be related to an altered metabolic and proteomic profile. | Patricia Prieto Rafael I Jaén Daniel Calle María Gómez-Serrano Estefanía Nú?ez María Fernández-Velasco Paloma Martín-Sanz Sergio Alonso Jesús Vázquez Sebastián Cerdán Miguel ángel Peinado Lisardo Boscá | 2019 | World Journal of Gastroenterology2019，25，4： | 4 |
| 5 | Evaluation of the bioavailability and metabolism in the rat of punicalagin, an antioxidant polyphenol from pomegranate juice显示文摘 | Bego?a Cerdá Rafael Llorach José J. Cerón Juan Carlos Espín Francisco A. Tomás-Barberán | 2003 | European Journal of Nutrition2003，，1： | 2 |
| 6 | Correlation between clinico-pathological outcome and typing of Haemophilus parasuis field strains显示文摘 | Virginia Aragon Marta Cerdà-Cuéllar Lorenzo Fraile Mark Mombarg Miquel Nofrarías Alexandre Olvera Marina Sibila David Solanes Joaquim Segalés | 2009 | Veterinary Microbiology2009，，3： | 2 |
| 7 | Volatile compounds from fontinalis antipyretica显示文摘 | Leif Svensson | 1971 | Phytochemistry1971，12，10： | 1 |
| 8 | Correlation between clinico - pathological outcome and typing of Haemophilus parasuis field strains显示文摘 | Aragon V Cerdà - Cuéllar M Fraile L | 2010 | Veterinary Microbiology2010，142，34： | 1 |
| 9 | Survival benefit in critically ill burned patients receiving selective decontamination of the digestive traet显示文摘 | Miguel A de La Cal Enrique Cerd~t Paloma Garc ~ a-Hierro | 2005 | Ann Surg2005，204，3： | 1 |
| 10 | Brain Region-Selective Mechanisms Contribute to the Progression of Cerebral Alterations in Acute Liver Failure in Rats显示文摘 | Omar Cauli Pilar López–Larrubia Regina Rodrigo Ana Agusti Jordi Boix Laura Nieto–Charques Sebastián Cerdán Vicente Felipo | 2011 | Gastroenterology2011，，2： | 1 |
| 11 | Assessment of the anti-inflammatory activity and free radical scavenger activity of tiliroside显示文摘 | Araceli Sala M.Carmen Recio Guillermo R Schinella Salvador Má?ez Rosa M Giner Miguel Cerdá-Nicolás José-Luis R??os | 2003 | European Journal of Pharmacology2003，，1： | 1 |
| 12 | Expression and role of Elovl4 elongases in biosynthesis of very long-chain fatty acids during zebrafish Danio rerio early embryonic development显示文摘 | Monroig O Rotllant J Cerdá-Reverter JM | 2010 | Biochim Biophys Acta2010，1801，： | 1 |
| 13 | On natural resource substitution显示文摘 | Francisco J. André Emilio Cerdá | 2006 | Resources Policy2006，，4： | 1 |
| 14 | Effects of rock fragment cover on soil infiltration,interrill runoff and erosion显示文摘 | CerdàA | 2001 | European Journal of Soil Science2001，52，1： | 1 |
| 15 | Modification of the response of saline stressed tomato plants by the correction of cation disorders显示文摘 | Carvajal M Cerd A inez V M | 2000 | Plant Growth Regulation2000，，30： | 1 |
| 16 | Modalities of continuous renal replacement therapy:technical and clinical considerations显示文摘 | Cerd A Ronco C | | 0，，02： | 1 |
| 17 | The potential of downscaled dynamic column extraction for fast and reliable assessment of natural weathering effects of municipal solid waste in- cineration bottom ashes 显示文摘 | Rosende M Mir M Cerd V | 2008 | Analytica Chimica Acta2008，619，： | 1 |
| 18 | Successive post - translational modifications of E - eadherin are required for In- 1A - mediated internalization of Listeria monocytogenes显示文摘 | BONAZZI M VEIGA E PIZARRO - CERD/ J | 2008 | Cell Mi- crobiol2008，10，1： | 1 |
| 19 | The contrasting characteristics of acute kidney injury in developed and developing countries显示文摘 | Cerdá J Bagga A Kher V | | 0，，： | 1 |
| 20 | An MILP framework for batch reactive scheduling with limited discrete resources 显示文摘 | Mendez C A Cerd J | 2004 | Computers&Chemical Engineering2004，28，67： | 1 |
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