|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Interplay between post-translational cyclooxygenase-2 modifications and the metabolic and proteomic profile in a colorectal cancer cohort显示文摘BACKGROUND Colorectal cancer(CRC) is the second most common cause of cancer death worldwide. It is broadly described that cyclooxygenase-2(COX-2) is mainly overexpressed in CRC but less is known regarding post-translational modifications of this enzyme that may regulate its activity, intracellular localization and stability. Since metabolic and proteomic profile analysis is essential for cancer prognosis and diagnosis, our hypothesis is that the analysis of correlations between these specific parameters and COX-2 state in tumors of a high number of CRC patients could be useful for the understanding of the basis of this cancer in humans.AIM To analyze COX-2 regulation in colorectal cancer and to perform a detailed analysis of their metabolic and proteomic profile.METHODS Biopsies from both healthy and pathological colorectal tissues were taken under informed consent from patients during standard colonoscopy procedure in the University Hospital of Bellvitge(Barcelona, Spain) and Germans Trias i Pujol University Hospital(Campus Can Ruti)(Barcelona, Spain). Western blot analysis was used to determine COX-2 levels. Deglycosylation assays were performed in both cells and tumor samples incubating each sample with peptide N-glycosidase F(PNGase F). Prostaglandin E2(PGE2) levels were determined using a specific ELISA. 1 H high resolution magic angle spinning(HRMAS) analysis was performed using a Bruker AVIII 500 MHz spectrometer and proteomic analysis was performed in a nano-liquid chromatography-tandem mass spectrometer(nano LC-MS/MS) using a QExactive HF orbitrap MS.RESULTS Our data show that COX-2 has a differential expression profile in tumor tissue of CRC patients vs the adjacent non-tumor area, which correspond to a glycosylated and less active state of the protein. This fact was associated to a lesser PGE2 production in tumors. These results were corroborated in vitro performing deglycosylation assays in HT29 cell line where COX-2 protein profile was modified after PNGase F incubation, showing higher PGE2 levels. Moreover,HRMAS analysis indicated that tumor tissue has altered metabolic features vs non-tumor counterparts, presenting increased levels of certain metabolites such as taurine and phosphocholine and lower levels of lactate. In proteomic experiments, we detected an enlarged number of proteins in tumors that are mainly implicated in basic biological functions like mitochondrial activity,DNA/RNA processing, vesicular trafficking, metabolism, cytoskeleton and splicing.CONCLUSION In our colorectal cancer cohort, tumor tissue presents a differential COX-2 expression pattern with lower enzymatic activity that can be related to an altered metabolic and proteomic profile. | Patricia Prieto Rafael I Jaén Daniel Calle María Gómez-Serrano Estefanía Nú?ez María Fernández-Velasco Paloma Martín-Sanz Sergio Alonso Jesús Vázquez Sebastián Cerdán Miguel ángel Peinado Lisardo Boscá | 2019 | World Journal of Gastroenterology2019，25，4： | 4 |
| 2 | Correlation between clinico - pathological outcome and typing of Haemophilus parasuis field strains显示文摘 | Aragon V Cerdà - Cuéllar M Fraile L | 2010 | Veterinary Microbiology2010，142，34： | 1 |
| 3 | Modification of the response of saline stressed tomato plants by the correction of cation disorders显示文摘 | Carvajal M Cerd A inez V M | 2000 | Plant Growth Regulation2000，，30： | 1 |
| 4 | The potential of downscaled dynamic column extraction for fast and reliable assessment of natural weathering effects of municipal solid waste in- cineration bottom ashes 显示文摘 | Rosende M Mir M Cerd V | 2008 | Analytica Chimica Acta2008，619，： | 1 |
| 5 | The contrasting characteristics of acute kidney injury in developed and developing countries显示文摘 | Cerdá J Bagga A Kher V | | 0，，： | 1 |
| 6 | Study of the population structure of Haemophilus parasuis by multilocus sequence typing显示文摘 | Olvera A Cerdà-Cué11ar M Aragon V | 2006 | Microbiology2006，152，12： | 1 |
| 7 | Correlation between clinico-pathological outcome and typing of Haemophilus parasuis field strains显示文摘 | Aragon V Cerdà-Cuéllar M Fraile L | 2010 | Vet Microbiol2010，142，34： | 1 |
| 8 | Speciation analysis of inorganic arsenic by a multisyringe flow injection system with hydride generation- atomic fluorescence spectrometric detection 显示文摘 | Leal L O Forteza R Cerd'a V | 2006 | Talanta2006，69，： | 1 |
| 9 | Demarcating speeifieion (NaC1 C1 , Na+ ) and osmotic effects in the response of two citrus ro- otstocks to salinity 显示文摘 | Ruiz D Martinez V Cerd A | 1999 | Scientia Horticulturae1999，80，34： | 1 |
| 10 | Speciation analysis of inorganic arsenic by a multisyringe flow injection system with hydride generation atomic fluorescence spectrometric detection 显示文摘 | Leal L O Forteza R Cerd'a V | 2006 | Talanta2006，，69： | 1 |
| 11 | Water and nutrient uptake of grafted tomato plants grown under saline conditions 显示文摘 | Femindez-Garcla N Martlnez V Cerd/t A | 2002 | Journal of Plant Physiology2002，159，8： | 1 |
| 12 | Development and testing of a new computerized method for muhicomponent kinetic determinations based on multiwavelength spectrophotometric detection 显示文摘 | Estela J M Cladera A Cerdà V | 1995 | Analytica Chimica Acta1995，310，2： | 1 |
| 13 | Demarcating specific ion (NaCl,Cl-,Na+) and osmotic effects in the response of two citrus rootstocks to salinity显示文摘 | Ruiz D Martinez V Cerdá A | 1999 | Scientia Horticulturae1999，80，34： | 1 |
| 14 | Simultaneous spectrophotometric determination of calcium and magnesium in water显示文摘 | Gómez E Estela J M Cerdà V | 1991 | Anal Chim Acta1991，249，： | 1 |
| 15 | Study of the population structure of Haemophilus parasuis by multilocus sequence typing显示文摘 | Olvera A Cerdà-Cuéllar M ARAGON V | 2006 | Microbiology2006，152，12： | 1 |
| 16 | Speciation analysis of inorganic arsenic by a multisyringe flow injection system with hydride generation-atomic fluorescence spectrometric detection显示文摘 | L O LEAL R FORTEZA V CERDà | 2006 | Talanta2006，，69： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员