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| 1 | Podoplanin binds ERM proteins to activate RhoA and promote epithelial-mesenchymal transition显示文摘 | Martín-Villar E Megías D Castel S | 2006 | J Cell Sci2006，119，21： | 1 |
| 2 | Sensitive markers used to identify compounds that trigger apoptosis in cultured hepatocytes显示文摘 | COMEZ-LECHON M J O CONNOR E CASTELL J V | 2002 | Toxicological Sciences2002，65，2： | 1 |
| 3 | Materials used as PCM in thermal energy storage in buildings: a review 显示文摘 | Cabeza E Castell A Barreneche C Graeia A Fernandez A | 2011 | Renewable and Sustainable Energy Reviews2011，15，3： | 1 |
| 4 | Develo- ping suitable colour in the gonads of cultured green sea urchins ( Strongylocentrotus droebachiensis) 显示文摘 | Robinson S M C Castell J D Kennedy E J | 2002 | Aq- uaculture2002，206，： | 1 |
| 5 | Feeding selectivity of the harpacticoid copepod Canuellaperplexa in benthic muddy environments demonstrated by HPLC analyses of chlorine and carotenoid pigments显示文摘 | Buffan Dubau E Wit R Castel J | 1996 | Mar Ecol Prog Ser1996，137，： | 1 |
| 6 | Colonoscopy versus fecal immuneoch-emical testing in colorectal cancer screening 显示文摘 | Quinlero E Castells A Bujanda L | 2012 | N Engl J Med2012，366，： | 1 |
| 7 | Acute spinal cord compression due to intraspinal bleeding from a vertebral hemangioma : two case reports 显示文摘 | Castel E Lazennec JY Chiras J | 1999 | Eur Spinal J1999，18，2： | 1 |
| 8 | Acute spinal cord compression due to intraspinal bleeding from a vertebral hemangioma:two case reports显示文摘 | Castel E LaTmllnec JY Chiras J | | 0，，03： | 1 |
| 9 | [Thromboprophylaxis with enoxaparin for total knee replacement:An observational, retrospective and multicentre study comparing starting the treatment before and after the operation]显示文摘 | Llau JV Gil-Garay E Castel et E | | 0，，06： | 1 |
| 10 | Head injuries:incidence and outcome显示文摘 | Coadon F Richer E Castel JP | 1991 | J Neurol Sci1991，，： | 1 |
| 11 | In s itu gas analysis of Li4Ti5O12 based electroldes at elevated temperatures显示文摘 | He M L Castel E Laumann A | 2015 | J Electrochem Soc2015，6，162： | 1 |
| 12 | RNA interference in the nucleus: roles for small RNAs in transcription, epige neties and beyond 显示文摘 | CASTEL S E MARTIENSSEN R A | 2013 | Nature Revievas Genetics2013，14，2： | 1 |
| 13 | Institutional change as an in- teractive process: the case of the modernization of the French cancer centers显示文摘 | CASTEL E P FRIEDBERG | 2010 | Organization Science2010，21，2： | 1 |
| 14 | Esomeprazole (40 mg) compared with lansoprazole (30 mg) in the treatment of erosive esophagitis显示文摘 | Castell D O Kahrilas P J Richter J E | 2002 | Am J Gastroenterol2002，97，： | 1 |
| 15 | Transient focal cerebral ischemia significantly alters not only EAATs but also VGLUTs expression in rats:relevance of changes in reactive astroglia显示文摘 | Sánchez-Mendoza E Burguete MC Castelló-Ruiz M | 2010 | J Neurochem2010，113，： | 1 |
| 16 | Are phenolic compounds released from the Mediterranean shrub Cistus albidus responsible for changes in N cycling in siliceous and calcareous soils? 显示文摘 | Castells E Penuelas J Valentine D W | 2004 | New Phytoll2004，162，1： | 1 |
| 17 | Retrospective study of 145 supernumerary teeth显示文摘 | Femandez Montenegro P Valmaseda Castell o n E Berini Aytes L | 2006 | Med Oral Patol Oral Cir Bucal2006，11，4： | 1 |
| 18 | Stratification analysis in packed bed thermal energy storage systems显示文摘 | ORO E CASTELL A | 2013 | Applied Energy2013，109，： | 1 |
| 19 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 20 | Podoplanin binds ERMproteins to activate RhoA and promote epithelial-mesenchymaltransition显示文摘 | Martín-Villar E Megías D Castel S | 2006 | J Cell Sci2006，119，21： | 1 |
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