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| 1 | Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells显示文摘AIM: To investigate and compare the hepatogenic transdifferentiation of adipose tissue-derived stem cells (ADSC) and bone marrow-derived mesenchymal stem cells (BMSC) in vitro. Transdifferentiation of BMSC into hepatic cells in vivo has been described. Adipose tissue represents an accessible source of ADSC, with similar characteristics to BMSC.METHODS: BMSCs were obtained from patients under-going total hip arthroplasty and ADSC from human adi-pose tissue obtained from lipectomy. Cells were grown in medium containing 15% human serum. Cultures were serum deprived for 2 d before cultivating under similar pro-hepatogenic conditions to those of liver develop-ment using a 2-step protocol with sequential addition of growth factors, cytokines and hormones. Hepatic differ-entiation was RT-PCR-assessed and liver-marker genes were immunohistochemically analysed. RESULTS: BMSC and ADSC exhibited a fibroblastic mor-phology that changed to a polygonal shape when cells differentiated. Expression of stem cell marker Thy1 de-creased in differentiated ADSC and BMSC. However, the expression of the hepatic markers, albumin and CYPs increased to a similar extent in differentiated BMSC and ADSC. Hepatic gene activation could be attributed to in-creased liver-enriched transcription factors (C/EBPβ and HNF4α), as demonstrated by adenoviral expression vec-tors.CONCLUSION: Mesenchymal stem cells can be induced to hepatogenic transdifferentiation in vitro. ADSCs have a similar hepatogenic differentiation potential to BMSC, but a longer culture period and higher proliferation ca-pacity. Therefore, adipose tissue may be an ideal source of large amounts of autologous stem cells, and may become an alternative for hepatocyte regeneration, liver cell transplantation or preclinical drug testing. | Raquel Taléns-Visconti Ana Bonora Ramiro Jover Vicente Mirabet Francisco Carbonell José Vicente Castell María José Gómez-Lechón | 2006 | World Journal of Gastroenterology2006，12，36： | 35 |
| 2 | Adjuvant therapy sparing in rectal cancer achieving complete response after chemoradiation显示文摘AIM:To evaluate the long-term results of conventional chemoradiotherapy and laparoscopic mesorectal excision in rectal adenocarcinoma patients without adjuvant therapy.METHODS:Patients with biopsy-proven adenocarcinoma of the rectum staged cT3-T4 by endoscopic ultrasound or magnetic resonance imaging received neoadjuvant continuous infusion of 5-fluorouracil for five weeks and concomitant radiotherapy.Laparoscopic surgery was planned after 5-8 wk.Patients diagnosed with ypT0N0 stage cancer were not treated with adjuvant therapy according to the protocol.Patients with ypT1-2N0 or ypT3-4 or N+were offered 5-fluorouracil-based adjuvant treatment on an individual basis.An external cohort was used as a reference for the findings.RESULTS:One hundred and seventy six patients were treated with induction chemoradiotherapy and 170underwent total mesorectal excision.Cancer staging of ypT0N0 was achieved in 26/170(15.3%)patients.After a median follow-up of 58.3 mo,patients withypT0N0 had five-year disease-free and overall survival rates of 96%(95%CI:77-99)and 100%,respectively.We provide evidence about the natural history of patients with localized rectal cancer achieving a complete response after preoperative chemoradiation.The inherent good prognosis of these patients will have implications for clinical trial design and care of patients.CONCLUSION:Withholding adjuvant chemotherapy after complete response following standard neoadjuvant chemoradiotherapy and laparoscopic mesorectal excision might be safe within an experienced multidisciplinary team. | Xabier García-Albéniz Rosa Gallego Ralf Dieter Hofheinz Gloria Fernández-Esparrach Juan Ramón Ayuso-Colella Josep Antoni Bombí Carles Conill Miriam Cuatrecasas Salvadora Delgado Angels Ginés Rosa Miquel Mario Pagés Estela Pineda Verónica Pereira Aarón Sosa Oscar Reig Iván Victoria Luis Feliz Antonio María de Lacy Antoni Castells Iris Burkholder Andreas Hochhaus Joan Maurel | 2014 | World Journal of Gastroenterology2014，20，42： | 5 |
| 3 | New genes emerging for colorectal cancer predisposition显示文摘Colorectal cancer(CRC)is one of the most frequent neoplasms and an important cause of mortality in the developed world.This cancer is caused by both genetic and environmental factors although 35%of the variation in CRC susceptibility involves inherited genetic differences.Mendelian syndromes account for about5%of the total burden of CRC,with Lynch syndrome and familial adenomatous polyposis the most common forms.Excluding hereditary forms,there is an important fraction of CRC cases that present familial aggregation for the disease with an unknown germline genetic cause.CRC can be also considered as a complex disease taking into account the common diseasecommom variant hypothesis with a polygenic model of inheritance where the genetic components of common complex diseases correspond mostly to variants of low/moderate effect.So far,30 common,low-penetrance susceptibility variants have been identified for CRC.Recently,new sequencing technologies including exomeand whole-genome sequencing have permitted to add a new approach to facilitate the identification of new genes responsible for human disease predisposition.By using whole-genome sequencing,germline mutations in the POLE and POLD1 genes have been found to be responsible for a new form of CRC genetic predisposition called polymerase proofreading-associated polyposis. | Clara Esteban-Jurado Pilar Garre Maria Vila Juan José Lozano Anna Pristoupilova Sergi Beltrán Anna Abulí Jenifer Muoz Francesc Balaguer Teresa Ocaa Antoni Castells Josep M Piqué Angel Carracedo Clara Ruiz-Ponte Xavier Bessa Montserrat Andreu Luis Bujanda Trinidad Caldés Sergi Castellví-Bel | 2014 | World Journal of Gastroenterology2014，20，8： | 3 |
| 4 | Surgical resection of hepatocellular carcinoma in cirrhotic patients: Prognostic value of preoperative portal pressure显示文摘 | J Bruix A Castells J Bosch F Feu J Fuster JC Garcia-Pagan J Visa C Bru J Rodes | 1996 | Gastroenterology1996，，4： | 3 |
| 5 | Identification of Lynch syndrome: How should we proceed in the 21^(st) century?显示文摘Lynch syndrome, also known as hereditary non-polyposis colorectal cancer (HNPCC), is the most common form of hereditary colorectal cancer. Although great advances in the understanding of its molecular basis have taken place in the last decade, optimal selection of individuals for HNPCC genetic testing remains controversial. This is especially relevant since colonoscopy has been proven effective for reducing colorectal cancer incidence and mortality in individuals at-risk for this disorder. In this manuscript, we summarize the most significant contributions to this important issue that have appearedin the last few years. | Antoni Castells Francesc Balaguer Sergi Castellví-Bel Victòria Gonzalo Teresa Ocaa | 2007 | World Journal of Gastroenterology2007，13，33： | 3 |
| 6 | A silver colloid produced by reduction with hydrazine as support for highly sensitive surface-enhanced Raman spectroscopy显示文摘 | Nickel U Castell A Poppl K | 2000 | Langmuir2000，16，23： | 1 |
| 7 | Pharyngeal and upper esophageal sphincter manometry in the evaluation of dysphatia显示文摘 | Hila A Castell JA Castell DO | 2001 | Clin Gastroenterol2001，33，： | 1 |
| 8 | Fatigue crack growth in pavements显示文摘 | CASTELL M A INGRAFFEA A R IRWIN L H | 2000 | Journal of Transportation Engineering ASCE2000，126，4： | 1 |
| 9 | Detection of colonic cells in peripheral blood of colorectal cancer patients by means of reverse transcription and polymerase chain reaction显示文摘 | Castells A Boix L Bessa X | 1998 | Br J Cancer1998，78，： | 1 |
| 10 | Materials used as PCM in thermal energy storage in buildings: a review 显示文摘 | Cabeza E Castell A Barreneche C Graeia A Fernandez A | 2011 | Renewable and Sustainable Energy Reviews2011，15，3： | 1 |
| 11 | Mechanical behavior model of corroded reinforced concrete 显示文摘 | Castel A Franqois R Arliguie G | 2002 | Comptes Rendus de 1'' Acad6mie des Sciences-S6rie IIb/Me- chanics2002，，330： | 1 |
| 12 | Concrete cover cracking with reinforcement corrosion of RC beam during chloride - in- duced corrosion process 显示文摘 | ZHANG R CASTEL A FRANCOIS R | 2010 | Cement and Concrete Research2010，40，3： | 1 |
| 13 | Pharyngeal and upper esoph- ageal sphincter manometry in the evaluation of dysphagia显示文摘 | Hila A Castell JA Castell DO | 2001 | J Clin Gastroenterol2001，33，5： | 1 |
| 14 | Value of postoperative surveillance after radical surgeryfor colorectal cancer: results of a cohort study显示文摘 | Castells A Bessa X Daniels M | 1998 | Dis Colon Reclum1998，41，6： | 1 |
| 15 | Floating thrombus in an aberrant right subclavian artery a rare cause of peripheral arterial embolic events 显示文摘 | Vinsonneau U Castellant P Traore A | 2008 | Rev Med In- terna2008，29，11： | 1 |
| 16 | A prospective trial comparing wireless capsule endoscopy and barium contrast series for small-bowel surveillance in hereditary GI polyposis syndromes 显示文摘 | Mata A Llach J Castells A | 2005 | Gastrointest Endosc2005，61，6： | 1 |
| 17 | Maximisation of heat transfer in a coil in tank PCM cold storage system显示文摘 | Castell A Belusko M Bruno F | 2011 | Applied Energy2011，88，11： | 1 |
| 18 | Materials used as PCM in thermal energy storage in buildings: A review 显示文摘 | Cabeza L F Castell A Barreneche C | 2011 | Renewable Sustainable Energy Rev2011，15，3： | 1 |
| 19 | Colonoscopy versus fecal immuneoch-emical testing in colorectal cancer screening 显示文摘 | Quinlero E Castells A Bujanda L | 2012 | N Engl J Med2012，366，： | 1 |
| 20 | Resection for hepatocellular carcinoma is a good option in Child-Turcotte-Pugh Class A patients with cirrhosis who are eligible for liver transplantation 显示文摘 | Margarit C Escartin A Castells L | 2005 | Liver Transpl2005，11，10： | 1 |
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