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| 1 | 半嵌套式PCR检测转几丁质酶基因烟草研究显示文摘用含卡那霉素的培养基首先对转几丁质酶基因烟草种子和烟苗进行初步筛选鉴定 ,再用 Western Blot进一步证实抗卡那霉素的转基因烟苗中外源转几丁质酶基因的表达。然后研究半嵌套式 PCR(Semi- nested PCR)检测转几丁质酶基因烟草植株及其烤后烟叶中的所转目的基因 ;利用限制性内切酶 H ind 对半嵌套式 PCR产物进行酶切 ,从而验证半嵌套式 PCR产物是否为真正的目的基因。研究结果表明 ,半嵌套式 PCR是一种快速。 | 时焦 M Naylor M L Edwards J I Cooper 韩锦峰 | 2002 | 中国烟草学报2002，8，2： | 8 |
| 2 | Cytoplasmic dynein:a key player in neurodegenerative and neurodevelopmental diseases显示文摘Cytoplasmic dynein is the most important molecular motor driving the movement of a wide range of cargoes towards the minus ends of microtubules.As a molecular motor protein,dynein performs a variety of basic cellular functions including organelle transport and centrosome assembly.In the nervous system,dynein has been demonstrated to be responsible for axonal retrograde transport.Many studies have revealed direct or indirect evidence of dynein in neurodegenerative diseases such as amyotrophic lateral sclerosis,Charcot-Marie-Tooth disease,Alzheimer’s disease,Parkinson’s disease and Huntington’s disease.Among them,a number of mutant proteins involved in various neurodegenerative diseases interact with dynein.Axonal transport disruption is presented as a common feature occurring in neurodegenerative diseases.Dynein heavy chain mutant mice also show features of neurodegenerative diseases.Moreover,defects of dynein-dependent processes such as autophagy or clearance of aggregation-prone proteins are found in most of these diseases.Lines of evidence have also shown that dynein is associated with neurodevelopmental diseases.In this review,we focus on dynein involvement in different neurological diseases and discuss potential underlying mechanisms. | CHEN Xiang-Jun XU Huan COOPER Helen M LIU Yaobo | 2014 | Science China(Life Sciences)2014，57，4： | 8 |
| 3 | 2型糖尿病患者采用血管紧张素转换酶抑制剂治疗后血清肌酐急性升高及其继续治疗对主要临床结局的影响显示文摘血管紧张素转换酶抑制剂开始治疗后血清肌酐急性升高≥30%时推荐停药。但血清肌酐升高后继续服药或停药对主要临床结局的长期影响仍不明确。在ADVANCE(糖尿病与血管疾病行动:培哚普利-吲达帕胺与达美康缓释片对照评估)试验中,11 140例糖尿病患者在6周活性导入期后随机采用培哚普利-吲达帕胺或安慰剂治疗。 | Ohkuma T Jun M Rodgers A Cooper ME Glasziou P Hamet P Harrap S Mancia G Marre M Neal B Perkovic V Poulter N Williams B Zoungas S Chalmers J Woodward M 赵狄 练桂丽 | 2019 | 中华高血压杂志2019，27，1： | 6 |
| 4 | Balloon kyphoplasty compared to vertebroplasty and nonsurgical management in patients hospitalised with acute osteoporotic vertebral compression fracture: a UK cost-effectiveness analysis显示文摘 | A. Svedbom L. Alvares C. Cooper D. Marsh O. Str?m | 2013 | Osteoporosis International2013，，1： | 5 |
| 5 | The Yin and Yang of Wnt/Ryk axon guidance in development and regeneration显示文摘In the developing embryo,nascent axons navigate towards their specific targets to establish the intricate network of axonal connections linking neurons within the mature nervous system.Molecular navigational systems comprising repulsive and attractive guidance cues form chemotactic gradients along the pathway of the exploring growth cone.Axon-bound receptors detect these gradients and determine the trajectory of the migrating growth cone.In contrast to their benevolent role in the developing nervous system,repulsive guidance receptors are detrimental to the axon’s ability to regenerate after injury in the adult.In this review we explore the essential and beneficial role played by the chemorepulsive Wnt receptor,Ryk/Derailed in axon navigation in the embryonic nervous system(the Yin function).Specifically,we focus on the role of Wnt5a/Rykmediated guidance in the establishment of two major axon tracts in the mammalian central nervous system,the corticospinal tract and the corpus callosum.Recent studies have also identified Ryk as a major suppressor of axonal regeneration after spinal cord injury.Thus,we also discuss this opposing aspect of Ryk function in axonal regeneration where its activity is a major impediment to axon regrowth(the Yang function). | CLARK Charlotte EJ LIU Yaobo COOPER Helen M | 2014 | Science China(Life Sciences)2014，57，4： | 4 |
| 6 | Human immunodeficiency virus-related primary central nervous system lymphoma: factors influencing survival in 111 patients显示文摘BACKGROUND: The current study evaluated factors influencing survival in patients diagnosed with human immunodeficiency virus (HIV)-related primary central nervous system lymphoma (PCNSL), with a focus on the effects of therapeutic radiotherapy (RT) and highly active antiretroviral therapy (HAART). METHODS: A retrospective chart review of patients with a diagnosis of HIV-related PCNSL at one of five university hospitals between 1987 and 1998 was performed. Clinical details including antiretroviral agent use, brain imaging scan results, RT use, and survival outcomes were recorded. RESULTS: One hundred eleven patients with HIV-related PCNSL were identified. The annual incidence decreased significantly between 1992 and 1995 and between 1996 and 1998 (P=0.04). | Newell ME Hoy JF Cooper SG DeGraaff B Grulich AE Bryant M Millar JL Brew BJ Quinn DI | 2004 | 中国神经肿瘤杂志2004，2，2： | 4 |
| 7 | Analysis of a Successful Immune Response against Hepatitis C Virus显示文摘 | Stewart Cooper Ann L Erickson Erin J Adams Joe Kansopon Amy J Weiner David Y Chien Michael Houghton Peter Parham Christopher M Walker | 1999 | Immunity1999，，4： | 3 |
| 8 | Immunological roulette: Luck or something more? Considering the connections between host and environment in TB显示文摘Accurate prediction of which patient will progress from a sub-clinical Mycobacterium tuberculosis infection to active tuberculosis represents an elusive,yet critical,clinical research objective.From the individual perspective,progression can be considered to be the product of a series of unfortunate events or even a run of bad luck.Here,we identify the subtle physiological relationships that can influence the odds of progression to active TB and how this progression may reflect directed dysbiosis in a number of interrelated systems.Most infected individuals who progress to disease have apparently good immune responses,but these responses are,at times,compromised by either local or systemic environmental factors.Obvious disease promoting processes,such as tissue-damaging granulomata,usually manifest in the lung,but illness is systemic.This apparent dichotomy between local and systemic reflects a clear need to define the factors that promote progression to active disease within the context of the body as a physiological whole.We discuss aspects of the host environment that can impact expression of immunity,including the microbiome,glucocorticoid-mediated regulation,catecholamines and interaction between the gut,liver and lung.We suggest the importance of integrating precision medicine into our analyses of experimental outcomes such that apparently conflicting results are not contentious,but rather reflect the impact of these subtle relationships with our environment and microbiota. | John E Pearl Mrinal Das Andrea M Cooper | 2018 | Cellular & Molecular Immunology2018，15，3： | 3 |
| 9 | Do age-associated changes of voltage-gated sodium channel isoforms expressed in the mammalian heart predispose the elderly to atrial fibrillation?显示文摘Atrial fibrillation(AF)is the most common cardiac arrhythmia worldwide.The prevalence of the disease increases with age,strongly implying an age-related process underlying the pathology.At a time when people are living longer than ever before,an exponential increase in disease prevalence is predicted worldwide.Hence unraveling the underlying mechanics of the disease is paramount for the development of innovative treatment and prevention strategies.The role of voltage-gated sodium channels is fundamental in cardiac electrophysiology and may provide novel insights into the arrhythmogenesis of AF.Na_v1.5 is the predominant cardiac isoform,responsible for the action potential upstroke.Recent studies have demonstrated that Na_v1.8(an isoform predominantly expressed within the peripheral nervous system)is responsible for cellular arrhythmogenesis through the enhancement of pro-arrhythmogenic currents.Animal studies have shown a decline in Na_v1.5 leading to a diminished action potential upstroke during phase 0.Furthermore,the study of human tissue demonstrates an inverse expression of sodium channel isoforms;reduction of Na_v1.5 and increase of Na_v1.8 in both heart failure and ventricular hypertrophy.This strongly suggests that the expression of voltage-gated sodium channels play a crucial role in the development of arrhythmias in the diseased heart.Targeting aberrant sodium currents has led to novel therapeutic approaches in tackling AF and continues to be an area of emerging research.This review will explore how voltage-gated sodium channels may predispose the elderly heart to AF through the examination of laboratory and clinical based evidence. | Emmanuel Isaac Stephanie M Cooper Sandra A Jones Mahmoud Loubani | 2020 | World Journal of Cardiology2020，12，4： | 3 |
| 10 | Inflammation and Alzheimer’s disease显示文摘 | Haruhiko Akiyama Steven Barger Scott Barnum Bonnie Bradt Joachim Bauer Greg M Cole Neil R Cooper Piet Eikelenboom Mark Emmerling Berndt L Fiebich Caleb E Finch Sally Frautschy W.S.T Griffin Harald Hampel Michael Hull Gary Landreth Lih–Fen Lue Robert Mrak | 2000 | Neurobiology of Aging2000，，3： | 2 |
| 11 | Laser-induced bulk damage in potassium dihydrogen phosphate crystal显示文摘 | SINGLETON M F COOPER J F ANDRESEN B D | 1988 | Appl Phys Lett1988，52，14： | 2 |
| 12 | 以水解蛋白为基础的皮革复鞣剂显示文摘复鞣工序赋予皮革特别的性质,如丰满性、紧实性、柔软性、弹性以及物理力学性能等。本研究的目的在于证明可以商业利用的以蛋白质为基础的皮革复鞣剂。实验中采用的是鞣制后的开边蓝湿牛皮,通过蛋白质基复鞣剂与其他合成鞣剂和植物鞣剂等作对比试验,考察了复鞣剂浓度、浴液pH值和温度的影响,具体表现在皮革厚度、柔软度、丰满性和力学性能的不同。水解蛋白比其他复鞣剂复鞣的皮革增厚能力弱,断裂伸长率比空白试验(没有复鞣)皮革和其他复鞣剂复鞣的皮革低,但水解胶原蛋白能赋予皮革更好的撕裂强度。相反,水解角蛋白粉复鞣的皮革弹性与其他复鞣剂复鞣的皮革相当。对柔软性来说,水解胶原蛋白复鞣的皮革比水解角蛋白复鞣的皮革和空白试验的皮革柔软。因此,实验结果表明,投资回收皮革工业副产物水解角蛋白和胶原蛋白的技术是可行的。水解蛋白作为一种新的材料用于皮革生产,可改进材料管理,减轻环境损害,提高皮革工业效率,促进持续发展。 | Míriam Cooper Soriene Bordignon Mariliz Gutterres 何学勤 | 2014 | 西部皮革2014，36，8： | 2 |
| 13 | Cuticle-degrading enzymes of entomopathogenic fungi :Regulation of productionof chitinolytic enzymes显示文摘 | STLEGER RJ COOPER R M CHARNLEY A K | 1986 | Gen Microbio1986，132，： | 1 |
| 14 | More objectives tools for the integration of postural comfort in automobile seat design显示文摘 | Judic J M Cooper J A Truchot P | 1993 | SAE Paper 9301131993，，： | 1 |
| 15 | Development of drought resistant cultivars using physiological and morphological traits in rice显示文摘 | Fukai S Cooper M | 1995 | Field Crops Research1995，40，： | 1 |
| 16 | Tall fescue genomic SSR markers:development and transferability across multiple grass species 显示文摘 | SAHA M C COOPER J D ROUF MIAN M A | 2006 | Theor Appl Genet2006，113，： | 1 |
| 17 | Polyhedral cone-ratio DEA models with an illustrative application to large commercial banks显示文摘 | Cooper W W Huang Z M | 1990 | Journal of Econometrics1990，46，： | 1 |
| 18 | Attenuation,persistence,and vaccine potential of an Edwardsiella ictaluri purA mutant显示文摘 | Lawrence M L Cooper R K Thune R L | 1997 | Infection and Immunity1997，65，11： | 1 |
| 19 | Postpartum depression and the mother - infant relationship in a South African peri - urban settlement 显示文摘 | Cooper PJ Tomlinson M Swartz L | 1999 | British Journal of Psychiatry1999，175，： | 1 |
| 20 | Use of genetic mouse models in the study of diabetic nephropathy显示文摘 | Allen T J Cooper M E Lan H Y | 2004 | Curr Diab Rep2004，4，6： | 1 |
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