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| 1 | TALEN-based generation of a cynomolgus monkey disease model for human microcephaly显示文摘基因在非人类的首领编辑可以为在人探索病原学和遗传上基于的神经病学的混乱的治疗学的策略导致珍贵模型。然而,仔细在人模仿病理学、行为的赤字的神经病学的混乱的一个猴子模型成功地还没被产生了。Microcephalin 1 (MCPH1 ) 在人的大脑,和智力迟钝伴随的 MCPH1 变化原因头小畸型的进化被含有。这里,我们产生了用抄写带 biallelic MCPH1 变化的一只 cynomolgus 猴子(Macaca fascicularis ) 像使活跃之物的受动器核酸酶。猴子概括了大多数在病人观察的重要临床的特征,包括在头圆周,早熟的染色体冷凝作用(PCC ) ,语料库 callosum 的发育不全和上面的手足 spasticity 的显著减小。而且,在变异的真皮的成纤维细胞的 MCPH1 的 overexpression 救了 PCC 症候群。这个猴子模型可以帮助我们阐明在人的头小畸型并且更好的致病的 MCPH1 的角色在首领大脑尺寸的进化理解这蛋白质的功能。 | Qiong Ke Weiqiang Li Xingqiang Lai Hong Chen Lihua Huang Zhuang Kang Kai Li Jie Ren Xiaofeng Lin Haiqing Zheng Weijun Huang Yunhan Ma Dongdong Xu Zheng Chen Xinming Song Xinyi Lin Min Zhuang Tao Wang Fengfeng Zhuang Jianzhong Xi Frank Fuxiang Mao Huimin Xia Bruce T Lahn Qi Zhou Shihua Yang Andy Peng Xiang | 2016 | Cell Research2016，26，9： | 13 |
| 2 | Metabolic Engineering of Plant-derived (E)-β-farnesene Synthase Genes for a Novel Type of Aphid-resistant Genetically Modified Crop Plants显示文摘Aphids are major agricultural pests that cause significant yield losses of crop plants each year.Excessive dependence on insec-ticides for long-term aphid control is undesirable because of the development of insecticide resistance,the potential negative effects on non-target organisms and environmental pollution.Transgenic crops engineered for resistance to aphids via a non-toxic mode of action could be an efficient alternative strategy.(E)-β-Farnesene (EβF) synthases catalyze the formation of EβF,which for many pest aphids is the main component of the alarm pheromone involved in the chemical communication within these species.EβF can also be synthesized by certain plants but is then normally contaminated with inhibitory compounds.Engineering of crop plants capable of synthesizing and emitting EβF could cause repulsion of aphids and also the attraction of natural enemies that use EβF as a foraging cue,thus minimizing aphid infestation.In this review,the effects of aphids on host plants,plants' defenses against aphid herbivory and the recruitment of natural enemies for aphid control in an agricultural setting are briefly introduced.Furthermore,the plant-derived EβF synthase genes cloned to date along with their potential roles in generating novel aphid resistance via genetically modified approaches are discussed. | Xiu-Dao Yu John Pickett You-Zhi Ma Toby Bruce Johnathan Napier Huw D.Jones Lan-Qin Xia | 2012 | Journal of Integrative Plant Biology2012，54，5： | 10 |
| 3 | Analysis of the Arabidopsis Floral Proteome:Detection of over 2000 Proteins and Evidence for Posttranslational Modifications显示文摘The proteome of the Arabidopsis flower has not been extensively studied previously. Here, we report a proteomic analysis of the wild type Arabidopsis flower. Using both two-dimensional electrophoresis/mass spectrometry (2-DGE/MS) and multi-dimensional protein identification technology (MudPIT) approaches, we identified 2 446 proteins. Although a single experiment or analysis uncovered only a subset of the proteins we identified, a combination of multiple experiments and analyses facilitated the detection of a greater number of proteins. When proteins are grouped according to RNA expression levels revealed by microarray experiments, we found that proteins encoded by genes with relatively high levels of expression were detected with greater frequencies. On the other hand, at the level of the individual gene/protein, there was not a good correlation between protein spot intensity and microarray values. We also obtained strong evidence for post-translational modification from 2-DGE and MudPIT data. We detected proteins that are annotated to function in protein synthesis, folding, modification, and degradation, as well as the presence of regulatory proteins such as transcription factors and protein kinases. Finally, sequence and evolutionary analysis of genes for active methyl group metabolisms suggests that these genes are highly conserved. Our results allow the formulation of hypotheses regarding post-translational regulation of proteins in the flower, providing new understanding about Arabidopsis flower development and physiology. | Baomin Feng Lianchao Li Xiaofan Zhou Bruce Stanley Hong Ma | 2009 | Journal of Integrative Plant Biology2009，51，2： | 2 |
| 4 | Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level显示文摘Mesenchymal stem cells(MSCs)have been cited as contributors to heart repair through cardiogenic differentiation and multiple cellular interactions,including the paracrine effect,cell fusion,and mechanical and electrical couplings.Due to heart–muscle complexity,progress in the development of knowledge concerning the role of MSCs in cardiac repair is heavily based on MSC–cardiomyocyte coculture.In conventional coculture systems,however,the in vivo cardiac muscle structure,in which rod-shaped cells are connected end-to-end,is not sustained;instead,irregularly shaped cells spread randomly,resulting in randomly distributed cell junctions.Consequently,contact-mediated cell–cell interactions(e.g.,the electrical triggering signal and the mechanical contraction wave that propagate through MSC–cardiomyocyte junctions)occur randomly.Thus,the data generated on the beneficial effects of MSCs may be irrelevant to in vivo biological processes.In this study,we explored whether cardiomyocyte alignment,the most important phenotype,is relevant to stem cell cardiogenic differentiation.Here,we report(i)the construction of a laser-patterned,biochip-based,stem cell–cardiomyocyte coculture model with controlled cell alignment;and(ii)single-cell-level data on stem cell cardiogenic differentiation under in vivo-like cardiomyocyte alignment conditions. | Zhen Ma Qiuying Liu Huaxiao Yang Raymond B Runyan Carol A Eisenberg Meifeng Xu Thomas K Borg Roger Markwald Yifei Wang Bruce Z Gao | 2013 | Light(Science & Applications)2013，2，1： | 2 |
| 5 | Hematoma volume as the major determinant of outcomes after intracerebral hemorrhage显示文摘 | LoPresti MA Bruce SS Camacho E | 2014 | J Neurol Sci2014，345，12： | 1 |
| 6 | Anti-CD1 ld integrin antibody treatment restores normal serotonergic projections to the dorsal,in- termediate, and ventral horns of the injured spinal cord显示文摘 | Oatway MA Chen Y Bruce JC | 2005 | J Neu rosci2005，25，3： | 1 |
| 7 | Blunt renal artery injury : incidence, diagnosis, and management 显示文摘 | Bruce LM Croce MA Santaniello JM | 2001 | Am Surg2001，67，6： | 1 |
| 8 | Association of socioeconomic status and CKD among African Americans: the Jackson Heart Study 显示文摘 | Bruce MA Beech BM Crook ED | 2010 | Am J Kidney Dis2010，55，6： | 1 |
| 9 | Type, Rather Than Number, of Mental and Physical Comorbidities Increases the Severity of Symptoms in Patients With Irritable Bowel Syndrome显示文摘 | Jeffrey M. Lackner Chang–Xing Ma Laurie Keefer Darren M. Brenner Gregory D. Gudleski Nikhil Satchidanand Rebecca Firth Michael D. Sitrin Leonard Katz Susan S. Krasner Sarah K. Ballou Bruce D. Naliboff Emeran A. Mayer | 2013 | Clinical Gastroenterology and Hepatology2013，，： | 1 |
| 10 | Inventorying heavy metal pollution in redeveloped brownfield and its policy contribution: Case study from Tiexi District, Shenyang, China显示文摘 | Wanxia Ren Bing Xue Yong Geng Lina Sun Zhixiao Ma Yunsong Zhang Bruce Mitchell Liming Zhang | 2014 | Land Use Policy2014，，： | 1 |
| 11 | Social environmental stressors, psychological factors, and kidney disease 显示文摘 | Bruce MA Beech BM Sims M | 2009 | J Inves- rig Med2009，57，4： | 1 |
| 12 | Self-assembling peptide detergents stabilize isolated photosystem I on a dry surface for an extended time 显示文摘 | Kiley P Zhao X Vaughn M Baldo MA Bruce BD Zhang S | 2005 | PLoS Biol2005，3，： | 1 |
| 13 | Tranexamic acid for intracerebral haemorrhage within 2 hours of onset: protocol of a phase Ⅱ randomised placebo-controlled double-blind multicentre trial显示文摘Rationale Haematoma growth is common early after intracerebral haemorrhage(ICH),and is a key determinant of outcome.Tranexamic acid,a widely available antifibrinolytic agent with an excellent safety profile,may reduce haematoma growth.Methods and design Stopping intracerebral haemorrhage with tranexamic acid for hyperacute onset presentation including mobile stroke units(STOP-MSU)is a phase Ⅱ double-blind,randomised,placebo-controlled,multicentre,international investigator-led clinical trial,conducted within the estimand statistical framework.Hypothesis In patients with spontaneous ICH,treatment with tranexamic acid within 2 hours of onset will reduce haematoma expansion compared with placebo.Sample size estimates A sample size of 180 patients(90 in each arm)would be required to detect an absolute difference in the primary outcome of 20%(placebo 39%vs treatment 19%)under a two-tailed significance level of 0.05.An adaptive sample size re-estimation based on the outcomes of 144 patients will allow a possible increase to a prespecified maximum of 326 patients.Intervention Participants will receive 1 g intravenous tranexamic acid over 10 min,followed by 1 g intravenous tranexamic acid over 8 hours;or matching placebo.Primary efficacy measure The primary efficacy measure is the proportion of patients with haematoma growth by 24±6 hours,defined as either≥33%relative increase or≥6 mL absolute increase in haematoma volume between baseline and follow-up CT scan.Discussion We describe the rationale and protocol of STOP-MSU,a phase Ⅱ trial of tranexamic acid in patients with ICH within 2 hours from onset,based in participating mobile stroke units and emergency departments. | Nawaf Yassi Henry Zhao Leonid Churilov Bruce C V Campbell Teddy Wu Henry Ma Andrew Cheung Timothy Kleinig Helen Brown Philip Choi Jiann-Shing Jeng Annemarei Ranta Hao-Kuang Wang Geoffrey C Cloud Rohan Grimley Darshan Shah Neil Spratt Der-Yang Cho Karim Mahawish Lauren Sanders John Worthington Ben Clissold Atte Meretoja Vignan Yogendrakumar Mai Duy Ton Duc Phuc Dang Nguyen Thai My Phuong Huy-Thang Nguyen Chung Y Hsu Gagan Sharma Peter J Mitchell Bernard Yan Mark W Parsons Christopher Levi Geoffrey A Donnan Stephen M Davis | 2022 | Stroke & Vascular Neurology2022，7，2： | 1 |
| 14 | Atypical electrocardiogram in acute pericarditis;characteristics and prevalence显示文摘 | Bruce MA Spodick DH | 1980 | J Electrocardiol1980，13，1： | 1 |
| 15 | Complete genome sequence of the ethanol producer Zymomonas mobilis NCIMB 11163 显示文摘 | Kouvelis VN Saunders E Brettin TS Bruce D Detter C Han C Typas MA Pappas KM | 2009 | J BacwrioL2009，191，22： | 1 |
| 16 | Beau's lines and pyogenic granulomas following hand trauma 显示文摘 | Price MA Bruce S Waidhofer W | 1994 | Cutis1994，54，4： | 1 |
| 17 | Genome sequence of the ethanol-producing Zymomonas mobilis subsp, mobilis lectotype ATCC 10988 显示文摘 | Pappas KM Kouvelis VN Saunders E Brettin TS Bruce D Detter C Balakireva M Hart C Savvakis G Kyrpides NC Typas MA | | J Bacterio/0，193，18： | 1 |
| 18 | An electroretinographic and molecular genetic study of X-linked cone degeneration显示文摘 | REICHEL E BRUCE AM SANDBERG MA | 1989 | Am J Ophthalmol1989，108，： | 1 |
| 19 | Interactive effects of dietary cholesterol and different saturated fatty acids on lipoprotein metabolism in the hamster 显示文摘 | Billett MA Bruce JS White DA | 2000 | Br J Nutr2000，84，4： | 1 |
| 20 | Horseshoe-shaped post-opera- tive alopecia following lengthy head and neck surgery显示文摘 | Bruce IA Simmons MA Hampal S | 2002 | J Laryn- gol Otol2002，116，3： | 1 |
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