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| 1 | Lipopolysaccharide induces and activates the Nalp3 inflammasome in the liver显示文摘AIM:To examine the activation of the Nalp3 inflammasome and its downstream targets following lipopolysaccharide(LPS) -induced stimulation in the liver. METHODS:Six-to-eight-week-old C57BL/6 chow fed mice were injected intraperitoneally with 0.5μg/g bodyweight LPS and sacrificed 2,4,6,18 or 24 h later. LPS-induced liver damage was confirmed by a biochemical assay to detect alanine aminotransferase(ALT) levels.To determine if LPS stimulation in the liver led to activation of the inflammasome,real-time quantitative polymerase chain reaction was used to evaluate the mRNA expression of components of the Nalp3 inflammasome.Enzyme-linked immunosorbent assays were used to determine the protein expression levels of several downstream targets of the Nalp3 inflammasome,including caspase-1 and two cytokine targets of caspase-1,interleukin(IL) -1βand IL-18. RESULTS:We found that LPS injection resulted in liver damage as indicated by elevated ALT levels.This was associated with a significant increase in both mRNA and protein levels of the proinflammatory cy-tokine tumor necrosis factor(TNF) -αin the liver,as well as increased levels of TNFs in serum.We showed that LPS stimulation led to upregulation of mRNA levels in the liver for all the receptor components of the inflammasome,including Nalp3,Nalp1,pannexin-1 and the adaptor molecule apoptosis-associated specklike,caspase recruitment domain-domain containing protein.We also found increased levels of mRNA and protein for caspase-1,a downstream target of the inflammasome.In addition,LPS challenge led to increased levels of both mRNA and protein in the liver for two cytokine targets of caspase-1,IL-1βand IL-18. Interestingly,substantial baseline expression of pre-IL1βand pre-IL-18 was found in the liver.Inflammasome and caspase-1 activation was indicated by the significant increase in the active forms of IL-1βand IL-18 after LPS stimulation. CONCLUSION:Our results show that the Nalp3 inflammasome is upregulated and activated in the liver in response to LPS stimulation. | Michal Ganz Timea Csak Bharath Nath Gyongyi Szabo | 2011 | World Journal of Gastroenterology2011，17，43： | 14 |
| 2 | Role of biosurfactants in bioremediation of oil pollution-a review显示文摘The energy resources mainly petroleum and petroleum hydrocarbons are major pollutants of the environment.The oil and oil products contamination may cause severe harm and hence,the attention has been remunerated in the development of alternative technologies for elimination of these contaminants.Biosurfactants were used in the remediation of oil pollution due to advantages such as biodegradability and low toxicity.The biosurfactants are produced from low cost substrates like agro-industrial wastes which reduce the cost of production.Biosurfactants and bioemulsifiers are amphiphilic compounds and are produced as extracellular or a part of the cell membrane by bacteria.The insight view,how hydrocarbons are degraded by microorganisms and thereby reduce the damage of ecosystem is highly essential to target the problem.Biofilms are the bacterial communities which protects the bacterial cells from various adverse conditions.The present review describes the biosurfactants and its synthesis from bacteria and also emphases on the role of surfactants in oil remediation. | Abraham Peele Karlapudi TCVenkateswarulu Jahnavi Tammineedi Lohit Kanumuri Bharath Kumar Ravuru Vijaya ramu Dirisala Vidya Prabhakar Kodali | 2018 | Petroleum2018，4，3： | 13 |
| 3 | Structural basis for blocking PD-l-mediated immune suppression by therapeutic antibody pembrolizumab显示文摘 | Zhenkun Na Siok Ping Yeo Sakshibeedu R Bharath Matthew W Bowler Esra Ballkci Cheng-I Wang Haiwei Song | 2017 | Cell Research2017，27，1： | 10 |
| 4 | 抗AGR2单克隆抗体18A4抑制细胞外AGR2诱导的肿瘤细胞活性显示文摘在两种自身不表达肿瘤相关蛋白AGR2的黑色素瘤细胞A375和B16-F10中加入AGR2以增强肿瘤细胞活性,观察以AGR2为靶点的单克隆抗体18A4对细胞外AGR2诱导的肿瘤细胞活性的抑制作用。用MTT法和克隆形成实验检测AGR2,18A4抗体单用和联合作用对细胞增殖能力的影响;流式细胞术检测AGR2,18A4抗体单用和联合作用对细胞周期的影响;划痕实验检测细胞迁移能力;鬼笔环肽染色检测细胞形态变化;Western blot和免疫荧光染色检测细胞内p53表达量。结果表明,单克隆抗体18A4能够抑制细胞外AGR2诱导的肿瘤细胞增殖速度加快,G1/S细胞周期转化,迁移速度加快和细胞形态的变化。Western blot和免疫荧光染色检测发现在化疗药物阿霉素处理的细胞中,细胞外AGR2降低细胞内p53的表达量,而18A4能够抑制这一现象。实验结果表明18A4能够抑制细胞外AGR2诱导的多种肿瘤细胞活性,是一种潜在的抗肿瘤药物。 | 于天弘 Siva Bharath MERUGU Hema NEGI 孙宋暄 丁云鹤 武正华 李大伟 | 2018 | 中国药科大学学报2018，49，2： | 3 |
| 5 | Credit ratings and the BIS capital adequacy reform agenda 显示文摘 | Altman E I Bharath S T Saunders A | 2002 | Journal of Banking & Finance2002，26，9： | 2 |
| 6 | Structural and mutational studies on substrate specificity and catalysis of Salmonella Typhimurium D-cysteine desulfhydrase显示文摘 | Bharath S R Bisht S Harijan R K | 2012 | PIx:S ONE2012，7，36： | 1 |
| 7 | Pharmacokinetics of the novel PAR-1 antagonist vorapaxar in patients with hepatic impairment显示文摘 | Paul Statkevich Teddy Kosoglou Richard A. Preston Bharath Kumar Fengjuan Xuan Craig Trusley James E. Schiller Ronald B. Langdon David L. Cutler | 2012 | European Journal of Clinical Pharmacology2012，，11： | 1 |
| 8 | Numeric study of limited diffraction, bandlimited, acousticwaves: a novel solution family of the homogeneous scalar wave equation显示文摘 | Li G Bharath A A | 1998 | Wave Motion1998，28，3： | 1 |
| 9 | Does Asymmetric Information Drive Capital Structure Decisions?显示文摘 | Bharath S Pasquariello P | 2009 | The Review of Financial Studies2009，22，8： | 1 |
| 10 | The Effect of Multiple Doses of Ketoconazole or Rifampin on the Single- and Multiple-Dose Pharmacokinetics of Vorapaxar显示文摘 | Teddy Kosoglou Paul Statkevich Bharath Kumar Fengjuan Xuan James E. Schiller Amy O. Johnson-Levonas Sophia Young David L. Cutler | 2013 | Journal of Clinical Pharmacology2013，，5： | 1 |
| 11 | A capacitated vehicle routing problem for just - in - time delivery 显示文摘 | VAIDYANATHAN Matson BHARATH S | 1999 | IIE Transactions1999，，31： | 1 |
| 12 | Correlates of psychiatric morbidity in patients with leprosy显示文摘 | Bharath S Shamasundar C Raghuram R | 2001 | Indian J Lepr2001，73，3： | 1 |
| 13 | Clinical and magnetic resonance imaging findings in patients with Leigh syndrome and SURF1 mutations显示文摘 | Kothari Sonam Nahid Akthar Khan Parayil Sankaran Bindu Arun B. Taly N. Gayathri M.M. Srinivas Bharath C. Govindaraju H.R. Arvinda Madhu Nagappa Sanjib Sinha K. Thangaraj | 2013 | Brain and Development2013，，： | 1 |
| 14 | Optimization of process parameters for deposition of Steelite on X45CrSi3 Steel by plasma transferred arc technique 显示文摘 | Ravi Bharath R Ramanathan R Sundararajan B | 2008 | Materials and Design2008，29，： | 1 |
| 15 | Accounting Quality and Debt Contracting显示文摘 | Bharath S T J Sunder S V Sunder | 2008 | Accounting Review2008，83，1： | 1 |
| 16 | Phase equilibria of supercritieal CO2-fatty oil component binary systems显示文摘 | Ragunath Bharath | 1993 | Fluid Phase Equilibria1993，83，： | 1 |
| 17 | Multiple Voltage and frequency scheduling for power minimization显示文摘 | Venkatesan Muthukumar Bharath Radhakrishnan Henry Selvaraj | 2005 | Journal of System Architecture 512005，，： | 1 |
| 18 | A tiling of phase-space through self-convolution显示文摘 | Bharath A A | 2000 | IEEE Transactions on Signal Processing2000，48，12： | 1 |
| 19 | Utility of resting f MRI and connectivity in patients with brain tumor显示文摘 | Manglore S Bharath RD Panda R | 2013 | Neurol India2013，61，2： | 1 |
| 20 | Simple and Efficient Backbone Algorithm for Caulating Connected Dominating Set in Wireless Ad-hoc Networks 显示文摘 | RAGHAVAN V RAGHAVAN A BHARATH R | 2007 | World Academy of Science Engineering and Tech- nology2007，3，： | 1 |
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