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| 1 | Macrophage secretory products induce an inflammatory phenotype in hepatocytes显示文摘AIM:To investigate the influence of macrophages on hepatocyte phenotype and function.METHODS:Macrophages were differentiated from THP-1 monocytes via phorbol myristate acetate stimulation and the effects of monocyte or macrophageconditioned medium on HepG2 mRNA and protein expression determined.The in vivo relevance of these findings was confirmed using liver biopsies from 147 patients with hepatitis C virus(HCV)infection.RESULTS:Conditioned media from macrophages,but not monocytes,induced a transient morphological change in hepatocytes associated with upregulation of vimentin(7.8±2.5-fold,P=0.045)and transforming growth factor(TGF)-β1(2.6±0.2-fold,P<0.001)and downregulation of epithelial cadherin(1.7±0.02-fold,P=0.017)mRNA expression.Microarray analysis revealed significant upregulation of lipocalin-2(17-fold,P <0.001)and pathways associated with inflammation,and substantial downregulation of pathways related to hepatocyte function.In patients with chronic HCV,realtime polymerase chain reaction and immunohistochemistry confirmed an increase in lipocalin-2 mRNA(F0 1.0 ±0.3,F1 2.2±0.2,F2 3.0±9.3,F3/4 4.0±0.8,P= 0.003)and protein expression(F1 1.0±0.5,F2 1.3± 0.4,F3/4 3.6±0.4,P=0.014)with increasing liver injury.High performance liquid chromatography-tandem mass spectrometry analysis identified elevated levels of matrix metalloproteinase(MMP)-9 in macrophageconditioned medium,and a chemical inhibitor of MMP-9 attenuated the change in morphology and mRNA expression of TGF-β1(2.9±0.2 vs 1.04±0.1,P<0.001) in macrophage-conditioned media treated HepG2 cells.In patients with chronic HCV infection,hepatic mRNA expression of CD163(F0 1.0±0.2,F1/2 2.8±0.3,F3/4 5.3±1.0,P=0.001)and MMP-9(F0 1.0±0.4,F1/2 2.8±0.3,F3/4 4.1±0.8,P=0.011)was significantly associated with increasing stage of fibrosis.CONCLUSION:Secreted macrophage products alter the phenotype and function of hepatocytes,with increased expression of inflammatory mediators,suggesting that hepatocytes actively participate in liver injury. | Michelle Melino Victoria L Gadd Gene V Walker Richard Skoien Helen D Barrie Dinesh Jothimani Leigh Horsfall Alun Jones Matthew J Sweet Gethin P Thomas Andrew D Clouston Julie R Jonsson Elizabeth E Powell | 2012 | World Journal of Gastroenterology2012，18，15： | 3 |
| 2 | New insights in preventive cardiology and cardiac rehabilitation显示文摘 | Barry A Franklin Justin E Trivax Thomas E Vanhecke | 2008 | Current Opinion in Cardiology2008，，5： | 2 |
| 3 | Influence of age in estimating maximal oxygen uptake显示文摘ObjectiveTo 在最大的氧举起的估计( EE )的错误上估计年龄的影响( VO 2最大)用性别和在自从估计,测试的周期测力计锻练特定方程的人口, VO 2最大与实质的 EE 被联系,经常超过20%, 18~91 年,可能,由于机械 efficiency.Methods1850 的内在的可变性,成年人(68%人)经历了最大的周期测力计测试的心肺的锻练。心和肺的健康(CRF ) 相对性别和年龄被估计[更年轻(18 ~ 35 年) ,中年(36 ~ 60 年) 并且更旧(>60 年)] 。VO 2 最大[mL·(kg· min )−1] 被对煤气的交换的评价直接测量并且估计了使用性别和人口特定方程。VO 2 最大和相关 EE 在三年龄特定、性特定的 groups.ResultsDirectly 测量的 VO 2 男人和女人的最大是 29.5 ±10.5 mL·(kg· min )−1 和 24.2 ±9.0 mL·(kg· min )−1(P <0.01 ) 。EE [mL·(kg· min ) 为男人和女人的 −1] 和百分比错误(% E ) 有类似的价值, 0.5 ±3.2 和 0.4 ±2.9 mL·(kg· min )−1, 和 − 0.8 ±13.1% 并且 − 1.7 ±15.4%(P >0.05 ) 分别地。分别地,为每个年龄组的 EE 和 % E 为男人:更年轻的 = 1.9 ±4.1 mL·(kg· min )−1 和 3.8 ±10.5% ,中年的 = 0.6 ±3.1 mL·(kg· min )−1 和 0.4 ±10.3% ,更旧的 =− 0.2 ±2.7 mL·(kg· min )−1 和 − 4.2 ±16.6%(P <0.01 ) ;并且为女人:更年轻的 = 1.2 ±3.1 mL·(kg· min )−1 和 2.7 ±10.0% ,中年的 = 0.7 ±2.8 mL·(kg· min )−1 和 0.5 ±11.1% ,更旧的 =-0.8 ±2.3 mL·(kg· min )−1 和 − 9.5 ±22.4%(P <0.01 ).ConclusionVO 2 最大在更年轻的年龄组被低估并且在老年组被过高估计。年龄显著地影响 VO 2 在男人和女人的最大并且当 CRF 用人口被估计时,应该被认为特定方程,而非直接测量了。 | Christina G de Souza Silva Barry A Franklin Daniel E Forman Claudio Gil S Araujo | 2016 | Journal of Geriatric Cardiology2016，13，2： | 2 |
| 4 | Deciphering the biology of My cobacterium tuberculosis from the complete genome sequence 显示文摘 | Cole S T Brosch R Parkhill J Gamier T Churcher C Harris D Gordon S V Eiglmeier K Gas S Barry C E Tekaia F Badcock K Basham D Brown D Chillingworth T Cormor R Davies R Devlin K FeltweU T Gentles S Hamlin N Holroyd S Hornsby T Jagels K Kroghs A McLean J Moule S Murphy L Oliver K Osborne J Quail M A Rafandream M A Rogers J Rutter S Seeger K Skelton J Squaraes R Squares S Sulston J E Taylo K Whitehead S Barrell B G | 1998 | Nature1998，393，： | 1 |
| 5 | Foreign direct investment and wages in domestic firms in Ireland: productivity spillovers versus labor-market crowding out 显示文摘 | Barry F Gorg H Strobl E | 2005 | International Journal of the Economics of Business2005，12，1： | 1 |
| 6 | What is effective treatment of depression in people with epilepsy? 显示文摘 | BARRY JJ JONESJE E | 2005 | Epilepsy Behav2005，6，4： | 1 |
| 7 | High precision sampling for chromatographic separation显示文摘 | Barry E B Stuart P C Leitner J E | 1973 | Anal Chem1973，45，14： | 1 |
| 8 | Accuracy of ACARS Wind and Temperature Observations Determined by Collocation显示文摘 | Stanley G Benjamin Barry E Schwartz | 1999 | Weather and Forecasting1999，14，： | 1 |
| 9 | A multi-center phase I study of clofambine, etopeside and cyclophosphamide in combination in pediatric patients with refractory or relapsed acute leukemia 显示文摘 | Hijiya N Gaynon P Barry E | | Leukemia0，23，12： | 1 |
| 10 | Influence of environmen- tal Vertical wind shear on the intensity of hurricanne-strength tropical cyclones in the Australian region显示文摘 | Linda A P Barry N H Noel E D | 2005 | Mon Wea Rev2005，133，： | 1 |
| 11 | Critical interplay be- tween parasite differentiation, host immunity, and antigenic variation in trypanosome infections显示文摘 | GJINI E HAYDON D T BARRY J D | 2010 | Am Nat2010，176，4： | 1 |
| 12 | The effect of re- suspending sediment contaminated with antifouling paint particles containing Irgarol 1051 on the marine macrophyte Ulva intestinalis 显示文摘 | Tollaurst L E Barry J Dyer R A | 2007 | Chemosphere2007，68，8： | 1 |
| 13 | Is the Timed Up and Go test a usefulpredictor of risk of falls in community dwelling older adults: a systematic review and meta-analysis 显示文摘 | Barry E Galvin R Keogh C | 2014 | BMC Geriatr2014，14，： | 1 |
| 14 | DT40 cells lacking the Ca2+ - binding protein annexin 5 are resistant to Ca2+ -dependent apoptosis 显示文摘 | Timothy E Debipriya D Barry Y | 2002 | PNAS2002，99，12： | 1 |
| 15 | Application of the Cornell net carbohydrate and protein model for cattle consuming forage 显示文摘 | Fox D G Barry M C Pitt R E | 1995 | Journal of Animal Science1995，73，： | 1 |
| 16 | Quantification of Precipitation measurement discontinuity induced by wind shields on national gauges 显示文摘 | Yang Daqing Barry E G John R M | 1999 | Water Resources Researach1999，35，2： | 1 |
| 17 | Parameter and process significance in mechanistic modeling of cellulose hydrolysis显示文摘 | Rotter B E Barry D A Gerhard J I Small J S | 2009 | Bioresource Technol2009，99，13： | 1 |
| 18 | Designing catastrophe bonds to securitize systemic risks in agriculture: The case of Georgia cotton 显示文摘 | Dmitry V James E Barry J | 2006 | Journal of Agriculture and Resource Economics2006，31，2： | 1 |
| 19 | Presurgical electroencephalographic patterns and outcome from anterior temporal lobectomy 显示文摘 | Sussman NM O'Connor MJ | 1992 | Arch Neurol1992，49，： | 1 |
| 20 | Prediction of ruminal volatile fatty acids and pH within thd net carbohydrate and protein system显示文摘 | Pitt R E Van Kessel J S Fox D G Pell A N Barry M C Van Soest P J | 1996 | Journal of Animal Science1996，74，： | 1 |
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