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| 1 | Diagnostic and prognostic potential of tissue and circulating long non-coding RNAs in colorectal tumors显示文摘Long non-coding RNAs(lncRNAs)are members of the non-protein coding RNA family longer than 200 nucleotides.They participate in the regulation of gene and protein expression influencing apoptosis,cell proliferation and immune responses,thereby playing a critical role in the development and progression of various cancers,including colorectal cancer(CRC).As CRC is one of the most frequently diagnosed malignancies worldwide with high mortality,its screening and early detection are crucial,so the identification of disease-specific biomarkers is necessary.LncRNAs are promising candidates as they are involved in carcinogenesis,and certain lncRNAs(e.g.,CCAT1,CRNDE,CRCAL1-4)show altered expression in adenomas,making them potential early diagnostic markers.In addition to being useful as tissue-specific markers,analysis of circulating lncRNAs(e.g.,CCAT1,CCAT2,BLACAT1,CRNDE,NEAT1,UCA1)in peripheral blood offers the possibility to establish minimally invasive,liquid biopsy-based diagnostic tests.This review article aims to describe the origin,structure,and functions of lncRNAs and to discuss their contribution to CRC development.Moreover,our purpose is to summarise lncRNAs showing altered expression levels during tumor formation in both colon tissue and plasma/serum samples and to demonstrate their clinical implications as diagnostic or prognostic biomarkers for CRC. | Orsolya Galamb Barbara K Barták Alexandra Kalmár Zsófia B Nagy Krisztina A Szigeti Zsolt Tulassay Peter Igaz Béla Molnár | 2019 | World Journal of Gastroenterology2019，25，34： | 18 |
| 2 | Direct reprogramming of human fibroblasts into dopaminergic neuron-like cells显示文摘外长的 dopaminergic 神经原(DA 神经原) 的移植是为治疗 Parkinson 的疾病(PD ) 的一条有希望的途径。然而,主要跌倒块是施主 DA 神经原的可靠来源的缺乏。这里,我们证明五 transcriptional 因素 Mash1, Ngn2, Sox2, Nurr1,和 Pitx3 的联合直接并且有效地装改编人成纤维细胞进 DA 像神经原的房间。为为 DA 神经原的各种各样的标记积极的染色的 reprogrammed 房间。他们也显示出典型 DA 举起和生产性质。而且,他们展出了 DA 神经原特定的 electrophysiological 侧面。最后,他们在一个老鼠 PD 模型提供了征兆的地势。因此,我们的直接 reprogrammed DA 像神经原的房间是为 PD 的房间代替治疗的有希望的来源。 | Xinjian Liu Fang Li Elizabeth A Stubblefield Barbara Blanchard Toni L Richards Gaynor A Larson Yujun He Qian Huang Aik-Choon Tan Dabing Zhang Timothy A Benke John R Sladek Nancy R Zahniser Chuan-Yuan Li | 2012 | Cell Research2012，22，2： | 14 |
| 3 | Aging related methylation influences the gene expression of key control genes in colorectal cancer and adenoma显示文摘AIM To analyze colorectal carcinogenesis and age-related DNA methylation alterations of gene sequences associated with epigenetic clock CpG sites. METHODS In silico DNA methylation analysis of 353 epigenetic clock Cp G sites published by Steve Horvath was performed using methylation array data for a set of 123 colonic tissue samples [64 colorectal cancer(CRC), 42 adenoma, 17 normal; GEO accession number: GSE48684]. Among the differentially methylated agerelated genes, secreted frizzled related protein 1(SFRP1) promoter methylation was further investigated in colonic tissue from 8 healthy adults, 19 normal children, 20 adenoma and 8 CRC patients using bisulfite-specific PCR followed by methylation-specific high resolution melting(MS-HRM) analysis. m RNA expression of age-related 'epigenetic clock' genes was studied using Affymetrix HGU133 Plus2.0 whole transcriptome data of 153 colonic biopsy samples(49 healthy adult, 49 adenoma, 49 CRC, 6 healthy children)(GEO accession numbers: GSE37364, GSE10714, GSE4183, GSE37267). Whole promoter methylation analysis of genes showing inverse DNA methylationgene expression data was performed on 30 colonic samples using methyl capture sequencing.RESULTS Fifty-seven age-related Cp G sites including hypermethylated PPP1R16 B, SFRP1, SYNE1 and hypomethylated MGP, PIPOX were differentially methylated between CRC and normal tissues(P < 0.05, ?β≥ 10%). In the adenoma vs normal comparison, 70 CpG sites differed significantly, including hypermethylated DKK3, SDC2, SFRP1, SYNE1 and hypomethylated CEMIP, SPATA18(P < 0.05, ?β≥ 10%). In MS-HRM analysis, the SFRP1 promoter region was significantly hypermethylated in CRC(55.0% ± 8.4 %) and adenoma tissue samples(49.9% ± 18.1%) compared to normal adult(5.2% ± 2.7%) and young(2.2% ± 0.7%) colonic tissue(P < 0.0001). DNA methylation of SFRP1 promoter was slightly, but significantly increased in healthy adults compared to normal young samples(P < 0.02). This correlated with significantly increased SFRP1 m RNA levels in children compared to normal adult samples(P < 0.05). In CRC tissue the mR NA expression of 117 agerelated genes were changed, while in adenoma samples 102 genes showed differential expression compared with normal colonic tissue(P < 0.05, logF C > 0.5). The change of expression for several genes including SYNE1, CLEC3 B, LTBP3 and SFRP1, followed the same pattern in aging and carcinogenesis, though not for all genes(e.g., MGP). CONCLUSION Several age-related DNA methylation alterations can be observed during CRC development and progression affecting the m RNA expression of certain CRC- and adenoma-related key control genes. | Orsolya Galamb Alexandra Kalmár Barbara Kinga Barták árpád V Patai Katalin Leiszter Bálint Péterfia Barnabás Wichmann Gábor Valcz Gábor Veres Zsolt Tulassay Béla Molnár | 2016 | World Journal of Gastroenterology2016，22，47： | 7 |
| 4 | 建立初级卒中中心的修订和更新意见来自脑卒中联盟的总结声明显示文摘背景和目的自从2000年脑卒中联盟发布首份关于建立初级卒中中心的推荐意见以来,初级卒中中心的组建和认证工作已出现迅速发展。本文的目的在于修订和更新我们关于初级卒中中心的推荐意见以反映最新资料和经验。方法我们使用MEDLINE和PubMed数据库回顾了相关文献(2000年3月至2011年1月),主要针对急性卒中的诊断、治疗和护理。如果发现有效且相关,同样对原始文献、汇总分析和其他治疗指南进行回顾并纳入分析。标明证据等级以反映当前指南发展的实际情况。结果基于文献回顾以及针对初级卒中中心的经验,一些要素的重要性已得到进一步的加强,同时增加了几个新的领域。这些内容包括:(1)急诊卒中团队的重要性;(2)配备遥测监控的卒中单元的重要性;(3)MRj和弥散加权序列进行脑成像的实施;(4)用磁共振血管成像或CT血管成像对脑血管系统进行评价;(5)心脏影像学检查;(6)康复治疗的早期启动;(7)由独立机构进行的认证,包括实地考察和疾病评价指标。结论基于上述证据,初级卒中中心的某些要素对于改善急性卒中患者的诊治特别重要。其他要素则集中于脑、脑血管系统和心脏的影像学检查。这些新的要素也许能改善在初级卒中中心接受诊治的卒中患者的治疗和转归。 | Mark J. Alberts Richard E. Latchaw Andy Jagoda Lawrence R Wechsler Todd Crocco Mary G. George E.S. Connolly Barbara Mancini Stephen Prudhomme Daryl Gress Mary E. Jensen Robert Bass Robert Ruff Kathy Foell Rocco A. Armonda Marian Emr Margo Warren Jim Baranski Michael D. Walker 黄志超(译) 张霞(译) 尤寿江(译) 曹勇军(译) 刘春风(译) | 2012 | 国际脑血管病杂志2012，20，7： | 3 |
| 5 | Role of endoscopic ultrasound in the molecular diagnosis of pancreatic cancer显示文摘Pancreatic ductal adenocarcinoma remains one of the most deadly types of tumor. Endoscopic ultrasoundguided fine-needle aspiration(EUS-FNA) is a safe, cost-effective, and accurate technique for evaluating and staging pancreatic tumors. However, EUS-FNA may be inconclusive or doubtful in up to 20% of cases. This review underlines the clinical interest of the molecular analysis of samples obtained by EUS-FNA in assessing diagnosis or prognosis of pancreatic cancer, especially in locally advanced tumors. On EUS-FNA materials DNA, mRNA and miRNA can be extracted, amplified, quantified and subjected to methylation assay. Kras mutation assay, improves diagnosis of pancreatic cancer. When facing to clinical and radiological presentations of pseudo-tumorous chronic pancreatitis, wildtype Kras is evocative of benignity. Conversely, in front of a pancreatic mass suspected of malignancy, a mutated Kras is highly evocative of pancreatic adenocarci-noma. This strategy can reduce false-negative diagnoses, avoids the delay of making decisions and reduces loss of surgical resectability. Similar approaches are conducted using analysis of miRNA expression as well as Mucin or markers of invasion(S100P, S100A6, PLAT or PLAU). Beyond the diagnosis approach, the prediction of response to treatment can be also investigated form biomarkers expression within EUS-FNA materials. | Barbara Bournet Marion Gayral Jérme Torrisani Janick Selves Pierre Cordelier Louis Buscail | 2014 | World Journal of Gastroenterology2014，20，31： | 3 |
| 6 | Transposon mouse models to elucidate the genetic mechanisms of hepatitis B viral induced hepatocellular carcinoma显示文摘The major type of human liver cancer is hepatocellular carcinoma(HCC), and there are currently many risk factors that contribute to this deadly disease. The majority of HCC occurrences are associated with chronic hepatitis viral infection, and hepatitis B viral(HBV) infection is currently a major health problem in Eastern Asia. Elucidating the genetic mechanisms associated with HBV-induced HCC has been difficult due to the heterogeneity and genetic complexity associated with this disease. A repertoire of animal models has been broadly used to study the pathophysiology and to develop potential treatment regimens for HBVassociated HCC. The use of these animal models has provided valuable genetic information and has been an important contributor to uncovering the factors involved in liver malignant transformation, invasion and metastasis. Recently, transposon-based mouse models are becoming more widely used in liver cancer research to interrogate the genome by forward genetics and also used to validate genes rapidly in a reverse genetic manner. Importantly, these transposon-based rapid reverse genetic mouse models could become crucial in testing potential therapeutic agents before proceeding to clinical trials in human. Therefore, this review will cover the use of transposon-based mouse models toaddress the problems of liver cancer, especially HBVassociated HCC occurrences in Asia. | Amy P Chiu Barbara R Tschida Lilian H Lo Branden S Moriarity Dewi K Rowlands David A Largaespada Vincent W Keng | 2015 | World Journal of Gastroenterology2015，21，42： | 2 |
| 7 | Killing of p53-deficient hepatoma cells by parvovirus H-1 and chemotherapeutics requires promyelocytic leukemia protein显示文摘AIM: To evaluate the synergistic targeting and killing of human hepatocellular carcinoma (HCC) cells lacking p53 by the oncolytic autonomous parvovirus (PV) H-1 and chemotherapeutic agents and its dependence on functional promyelocytic leukemia protein (PML). METHODS: The role of p53 and PML in regulating cy-totoxicity and gene transfer mediated by wild-type (wt) PV H-1 were explored in two pairs of isogenic human hepatoma cell lines with different p53 status. Further-more,H-1 PV infection was combined with cytostatic drug treatment. RESULTS: While the HCC cells with different p53 status studied were all susceptible to H-1 PV-induced apoptosis,the cytotoxicity of H-1 PV was morepronounced in p53-negative than in p53-positive cells. Apoptosis rates in p53-negative cell lines treated by genotoxic drugs were further enhanced by a treatment with H-1 PV. In flow cytometric analyses,H-1 PV infection resulted in a reduction of the mitochondrial transmembrane potential. In addition,H-1 PV cells showed a significant increase in PML expression. Knocking down PML expression resulted in a striking reduction of the level of H-1 PV infected tumor cell death. CONCLUSION: H-1 PV is a suitable agent to circumvent the resistance of p53-negative HCC cells to genotoxic agents,and it enhances the apoptotic process which is dependent on functional PML. Thus,H-1 PV and its oncolytic vector derivatives may be considered as therapeutic options for HCC,particularly for p53-negative tumors. | Maike Sieben Kerstin Herzer Maja Zeidler Vera Heinrichs Barbara Leuchs Martin Schuler Jan J Cornelis Peter R Galle Jean Rommelaere Markus Moehler | 2008 | World Journal of Gastroenterology2008，14，24： | 2 |
| 8 | Technical outcomes of sentinel-lymph-node resection and conventional axillary-lymph-node dissection in patients with clinically node-negative breast cancer: results from the NSABP B-32 randomised phase III trial显示文摘 | David N Krag Stewart J Anderson Thomas B Julian Ann M Brown Seth P Harlow Takamaru Ashikaga Donald L Weaver Barbara J Miller Lynne M Jalovec Thomas G Frazier R Dirk Noyes André Robidoux Hugh MC Scarth Denise M Mammolito David R McCready Eleftherios P Mamo | 2007 | Lancet Oncology2007，，10： | 2 |
| 9 | Intestinal inflammation and activation of sensory nerve pathways:a functional and morphological study in the nematode infected rat 显示文摘 | Giorgio R Barbara G Blennerhassett P | 2001 | Gut2001，49，： | 2 |
| 10 | Gastric hyperplastic polyps causing upper gastrointestinal hemorrhage in a young adult显示文摘Here, we report a case of a young man who presented with a significant upper gastrointestinal bleed treated by endoscopic removal of multiple hyperplastic polyps. Gastric hyperplastic polyps are a relatively uncommon cause of overt gastrointestinal bleeding. While most hyperplastic gastric polyps are asymptomatic, they may present with abdominal pain, iron def iciency anemia or gastric outlet obstruction. These polyps are associated with conditions such as Helicobacter pylori gastritis and atrophic autoimmune gastritis, which predispose the epithelium to chronic inf lammation and epithelial repair.The patient presented to Northwestern Memorial Hospital in July 2011. The polyps were resected by clip-assisted snare polypectomy. Histopathologic assessment of the resected polyps demonstrated multiple, nonulcerative hyperplastic polyps measuring 1.3-1.8 cm in size, without evidence of dysplasia or malignancy. This case describes a young adult patient with multiple, large gastric polyps causing overt gastrointestinal bleeding. This is a rare presentation in a young individual, as these polyps are typically identifi ed in patients older than 60 years of age and less commonly, pediatric populations. | Brian J Secemsky Kenika R Robinson Kumar Krishnan Kristina A Matkowskyj Barbara H Jung | 2013 | World Journal of Clinical Cases2013，1，1： | 2 |
| 11 | Organizational demography : the difference effects of age and tenure distributions on technical communication 显示文摘 | Zenger Todd R Barbara S Lawrence | 1989 | Academy of Management Journal1989，32，： | 1 |
| 12 | Conditions for stability of the extended Kalman filter and their application to the frequency trackingproblem显示文摘 | Barbara F Scala L Robert R B | 1995 | Mathematics of Control Signals and Systems1995，8，1： | 1 |
| 13 | Kinetics of the hydrolysis of Atlantic salmon (Salmo salar) muscle proteins by alkaline proteases and a visceral serine protease mixture显示文摘 | Hordur G K Barbara A R | 2000 | Process Biochem2000，36，12： | 1 |
| 14 | Optimizing contrast resolution in industrial computed tomographic scanners显示文摘 | Krohn Barbara R Silver Michael D | 1990 | Materials Evaluation1990，，10： | 1 |
| 15 | Evaluation of a transcription-mediated amplification-based HCV and HIV-1RNA duplex assay for screening individual blood donations: a comparison with a minipool testing system 显示文摘 | DANIEL C ALINE R BARBARA C | 2003 | Transfusion2003，43，2： | 1 |
| 16 | Having, giving, and getting: slack resources, corporate philanthropy and firm financial performance显示文摘 | SEIFERT BRUCE MORRIS SARA A BARTKUS BARBARA R | 2004 | Business & Society2004，43，2： | 1 |
| 17 | Evaluation of a candidate International Standard for Meningococcal Group C poly- saeeharide 显示文摘 | CAROLINE V BARBARA M PETER R | 2012 | Biologlcals2012，40，5： | 1 |
| 18 | Synthesis and antimycotic activity of new unsymmetrical substituted zinc phthalocyanines显示文摘 | Barbara C Gabrio R Donata D | 2003 | Tetrahedron2003，59，10： | 1 |
| 19 | Uranium reduction by desulfovibrio desulfuricans strain G20 and a cytochrome c3 mutant 显示文摘 | Rayford B P Darren M G Barbara J R | 2002 | Applied and Environmental Microbiology2002，68，6： | 1 |
| 20 | Development of continuous liver cell cultures from marine teleost,spot croaker (Leiostomus xanthurus,Pisces:Sciaenidae)显示文摘 | Mohamed F Barbara J R Susanne S D | 1995 | Aquaculture1995，132，： | 1 |
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