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| 1 | Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis显示文摘AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network.RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4. | Marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva | 2018 | World Journal of Gastroenterology2018，24，47： | 15 |
| 2 | Chronic exposure to ethanol causes steatosis and inflammation in zebrafish liver显示文摘AIM To evaluate the effects of chronic exposure to ethanol in the liver and the expression of inflammatory genesin zebrafish.METHODS Zebrafish(n = 104),wild type,adult,male and female,were divided into two groups:Control and ethanol(0.05 v/v).The ethanol was directly added into water;tanks water were changed every two days and the ethanol replaced.The animals were fed twice a day with fish food until satiety.After two and four weeks of trial,livers were dissected,histological analysis(hematoxilineosin and Oil Red staining) and gene expression assessment of adiponectin,adiponectin receptor 2(adipor2),sirtuin-1(sirt-1),tumor necrosis factor-alpha(tnf-a),interleukin-1b(il-1b) and interleukin-10(il-10) were performed.Ultrastructural evaluations were conducted at fourth week.RESULTS Exposing zebrafish to 0.5% ethanol developed intense liver steatosis after four weeks,as demonstrated by oil red staining.In ethanol-treated animals,the main ultrastructural changes were related to cytoplasmic lipid particles and droplets,increased number of rough endoplasmic reticulum cisterns and glycogen particles.Between two and four weeks,hepatic mR NA expression of il-1b,sirt-1 and adipor2 were upregulated,indicating that ethanol triggered signaling molecules which are key elements in both hepatic inflammatory and protective responses.Adiponectin was not detected in the liver of animals exposed and not exposed to ethanol,and il-10 did not show significant difference.CONCLUSION Data suggest that inflammatory signaling and ultrastructural alterations play a significant role during hepatic steatosis in zebrafish chronically exposed to ethanol. | Ana Claudia Reis Schneider Cleandra Gregório Carolina Uribe-Cruz Ranieli Guizzo Tais Malysz Maria Cristina Faccioni-Heuser Larisse Longo Themis Reverbel da Silveira | 2017 | World Journal of Hepatology2017，9，8： | 7 |
| 3 | Cirrhosis in children and adolescents: An overview显示文摘Several conditions, especially chronic liver diseases, can lead to cirrhosis in children and adolescents. Most cases in clinical practice are caused by similar etiologies. In infants, cirrhosis is most often caused by biliary atresia and genetic-metabolic diseases, while in older children, it tends to result from autoimmune hepatitis,Wilson's disease, alpha-1-antitrypsin deficiency and primary sclerosing cholangitis. The symptoms of cirrhosis in children and adolescents are similar to those of adults. However, in pediatric patients, the first sign of cirrhosis is often poor weight gain. The complications of pediatric cirrhosis are similar to those observed in adult patients, and include gastrointestinal bleeding caused by gastroesophageal varices, ascites and spontaneous bacterial peritonitis. In pediatric patients, special attention should be paid to the nutritional alterations caused by cirrhosis, since children and adolescents have higher nutritional requirements for growth and development. Children and adolescents with chronic cholestasis are at risk for several nutritional deficiencies. Malnutrition can have severe consequences for both pre- and post-liver transplant patients. The treatment of cirrhosis-induced portal hypertension in children and adolescents is mostly based on methods developed for adults. The present article will review the diagnostic and differential diagnostic aspects of end-stage liver disease in children, as well as the major treatment options for this condition. | Raquel Borges Pinto Ana Claudia Reis Schneider Themis Reverbel da Silveira | 2015 | World Journal of Hepatology2015，7，3： | 3 |
| 4 | Association between EGF +61A/G polymorphism and gastric cancer in Caucasians显示文摘AIM: To investigate the association between epidermal growth factor (EGF) +61A/G polymorphism and susceptibility to gastric cancer, through a cross-sectional study. METHODS: Polymerase chain reaction resctriction fragment lenght polymorphism analyses were used to genotype EGF +61 in 207 patients with gastric lesions (162 patients with gastric adenocarcinomas, 45 with atrophy or intestinal metaplasia) and 984 controls. All subjects were Caucasian. RESULTS: Genotype distribution was 23.5% for GG and 76.5% for GA/AA in the control group, 18.4% for GG and 68.6% for GA/AA in the entire group with gastric lesions and 17.9% for GG and 82.1% for GA/AA in the group with gastric adenocarcinoma. No statistically significant associations were found between EGF +61 variants and risk for developing gastric cancer [odds ratios (OR) = 1.41, 95% confidence intervals (CI): 0.90-2.21, P = 0.116]. However, the stratification of individuals by gender revealed that males carrying A alleles (EGF +61A/G or AA) had an increased risk for developing gastric cancer as compared to GG homozygous males (OR = 1.55, 95% CI: 1.05-2.28, P = 0.021). CONCLUSION: In summary, we found that males who were A carriers for EGF +61 had an increased risk for developing gastric cancer. This result may be explained by the suggestion that women secrete less gastric acid than men. | Ana Paula Araújo Bruno M Costa Ana L Pinto-Correia Maria Fragoso Paula Ferreira Mário Dinis-Ribeiro Sandra Costa Rui M Reis Rui Medeiros | 2011 | World Journal of Gastroenterology2011，17，4： | 3 |
| 5 | Acaricidal activity of Derris floribunda essential oil and its main constituent显示文摘Objective: To evaluate the acaricidal activity of the essential oil obtained from roots of Derris floribunda(D. floribunda)(Miq.) Benth, and its main constituent nerolidol against the Mexican mite Tetranychus mexicanus(T. mexicanus)(Mc Gregor).Methods: The essential oil from the roots of D. floribunda collected in the Amazon region(Brazil) was obtained by hydrodistillation. Its chemical composition was determined by GC–MS analysis. The acaricidal activities of this essential oil and nerolidol,were evaluated by recording the number of dead females(mortality) and eggs(fertility).Results: The essential oil showed sesquiterpenes as major volatile components. Nerolidol, the main component, represented 68.5% of the total composition of the essential oil. D. floribunda essential oil and nerolidol showed acaricidal activity, with LC_(50) of9.61 mg/m L air and 9.2 mg/m L air, respectively, over a 72 h period. In addition, both the essential oil and nerolidol significantly reduced the fecundity of T. mexicanus.Conclusions: Due to the economic importance of T. mexicanus and the lack of new pesticides, our data are very promising in the search for efficient and safer acaricidal products. Furthermore, this is the first report about the chemical composition and bioactivity of the essential oil of the Amazon plant species D. floribunda. | Ana Claudia Fernandes Amaral Aline de SRamos Marcia Reis Pena Jose Luiz Pinto Ferreira Jean Michel SMenezes Geraldo JNVasconcelos Neliton Marques da Silva Jefferson Rocha de Andrade Silva | 2017 | Asian Pacific Journal of Tropical Biomedicine2017，7，9： | 2 |
| 6 | Detection of contaminants in cell cultures, sera and trypsin显示文摘 | Tatiana Flávia Pinheiro de Oliveira Ant?nio Augusto Fonseca Marcelo Fernandes Camargos Anapolino Macedo de Oliveira Ana Cláudia Pinto Cottorello Antonizete dos Reis Souza Iassudara Garcia de Almeida Marcos Bryan Heinemann | 2013 | Biologicals2013，，6： | 2 |
| 7 | Topographic expression of MUC5AC and MUC6 in the gastric mucosa infected by Helicobacter pylori and in associated diseases显示文摘 | Terezinha Marques Leonor David Celso Reis Ana Nogueira | 2005 | Pathology - Research and Practice2005，，10： | 1 |
| 8 | The cardiovascular aspects of a Ménière’s disease population——A pilot study显示文摘Introduction:MD is considered a rare disease.An adequate model that explains MD's pathophysiology is not well established.Recently,the vascular theory of the disease has been revived.Objectives:To characterize a MD population according to its cardiovascular risk and correlate it to the MD clinical course.Methods:In this retrospective chart study the data of 31 MD patients observed between January 2017 and April 2018 in a tertiary university hospital were reviewed.Patients included in the study were diagnosed according to the Barany Society criteria.Lost follow-ups,patients with autoimmune disease,atopy or allergy,major psychiatric disease and migraine were excluded.Age,gender,cardiovascular risk factors,audiometric and vestibular parameters,occurrence of MD attacks in the previous 6 months,vestibular medication in course and time course of MD were recorded and compared between groups(with and without cardiovascular risk factors).Results:31 patients(61.3%females)mean aged 60.3 years(±9.7)were studied.74%of the population had at least one risk factor and 51.6%of patients had attacks in the last 6 months.There was a statistically significant difference in the occurrence of MD attacks in the last 6 months(p=0.014)between MD patients with and without risk factors.Mean PTA thresholds were higher and speech discrimination was lower in individuals with more cardiovascular risk factors.Conclusions:Treatment of MD focusing on vascular risk factors may allow a better control of symptoms and result in a decreased need for ablative procedures in this disorder. | Angela Reis Rego David Dias Ana Pinto SANDra Sousa e Castro Telma Feliciano Cecília Almeida e Sousa | 2019 | Journal of Otology2019，14，2： | 1 |
| 9 | Immunohistochemical expression of estrogen and progesterone receptors in endometrial polyps and adjacent endometrium in postmenopausal women显示文摘 | Sant'Ana de Almeida EC Nogueira AA Candido dos Reis FJ | 2004 | Maturitas2004，49，3： | 1 |
| 10 | Effect of ripening on texture, microstructure and cell wall polysaccharide composition of olive fruit ( Olea europaea ) 显示文摘 | Isabel Mafra Barbara Lanza Ana Reis | 2001 | Physiologia Plantarum2001，111，： | 1 |
| 11 | Local cytokine profiles of patients with cervical intraepithelial and invasive neoplasia显示文摘 | Bethanea Crema Peghini Douglas Reis Abdalla Ana Cristina Macedo Barcelos Lívia das Gra?as Vieito Lombardi Teodoro Eddie Fernando Candido Murta Márcia Antoniazi Michelin | 2012 | Human Immunology2012，，9： | 1 |
| 12 | Novel 3D scaffolds of chitosan-PLLA blends for tissue engineering applications:Preparation and characterization显示文摘 | Ana Rita C.Duarte Joao F Mano Rui L Reis | | 0，，： | 1 |
| 13 | 家猪肌内注射和接触传染感染非洲猪瘟病毒后的带毒及排毒动力学研究显示文摘非洲猪瘟病毒(African Swine Fever Virus,ASFV)是猪的一种强毒型病原体,从2007年起在东欧流行,目前还没有有效的疫苗或治疗方法。ASF病毒是当前的流行毒株,在猪体内带毒和排毒(shedding and excretion)的动力学机理至今尚不清楚。因此,本试验希望通过将易感的家猪与经肌内注射感染ASF病毒格鲁吉亚2007/1毒株的家猪进行接触,以测定ASF病毒在猪栏内和猪栏之间由受感染猪向易感猪进行传播的动力学机理。试验采集了血液、口液、鼻液、肠液(直肠)样本,以通过病毒滴定法和实时定量PCR(q PCR)法检测ASF病毒,利用血清学试验测定ASF病毒特异性抗体。肌内注射和接触传播感染均能造成猪急性发病,不过试验表明因感染途径不同而导致猪的发病机制有所差异。接种感染猪在接种的第3天可在血液中分离到有传染性的ASF病毒,而通过直接接触和间接接触进行感染的猪分别在曝露后第10天和第13天方可从血液中分离到有传染性的ASF病毒。临近出现ASF临床症状时,ASF病毒在血液中的滴度高于在鼻液和肠液中的。口液样本中没有分离到有传染性的ASF病毒,不过ASF病毒的基因组被检测到。只有一头接种感染的猪在接种后第12天检测到ASF病毒特异性抗体。本项试验结果提供了ASF病毒格鲁吉亚2007/1毒株在家猪中带毒和排毒的定量数据,同时表明该毒株引起持续感染的能力有限。 | 刘新军 张涛 Claire Guinat Ana Luisa Reis Christopher L.Netherton Lynnette Goatley Dirk U Pfeiffer Linda Dixon | 2015 | 国外畜牧学(猪与禽)2015，35，3： | 1 |
| 14 | Recombinant Antigen Targets for Serodiagnosis of African Swine Fever显示文摘 | Carmina Gallardo Ana Lui sa Reis Gladys Kalema-Zikusoka | 2009 | Clinical and Vaccine Immunology2009，16，7： | 1 |
| 15 | Aldobiouronic acid domains in helicobacter pylori显示文摘 | JoséA Ferreira Rosário M Domingues Ana Reis | 2011 | Carbohydrate Research2011，346，63： | 1 |
| 16 | Nursing Activities Score in the intensive care unit: Analysis of the related factors显示文摘 | Katia Grillo Padilha Regina Márcia Cardoso de Sousa Alda Ferreira Queijo Ana Márcia Mendes Dinis Reis Miranda | 2007 | Intensive & Critical Care Nursing2007，，3： | 1 |
| 17 | Liquid - liquid extraction procedure exploiting multieommutation in flow system for the determination of molybdenum in plants显示文摘 | Ana Lucia D Comitre Boaventura F Reis | 2003 | Analytica Chimica Acta2003，479，： | 1 |
| 18 | Automatic flow procedure based on multicommutation exploiting liquid-liquid extraction for spectrophotometric lead determination in plant material显示文摘 | ANA LUCIA D COMITRE BOAVENTURA F REIS | 2005 | Talanta2005，65，： | 1 |
| 19 | Crypthecodinium cohnii with emphasis on DHA production:a review显示文摘 | Ana Mendes Alberto Reis Rita Vasconcelos | 2009 | Journal of applied phycology2009，21，2： | 1 |
| 20 | Recombinant human erythropoietin treatment protects the cardio-renal axis in a model of moderate chronic renal failure显示文摘 | Ana Margarida Teixeira Patrícia Garrido Paulo Santos Rui Alves Belmiro Parada Elísio Costa Anabela Almeida Edite Teixeira-Lemos José Sereno Rui Pinto Luís Belo Alice Santos-Silva Frederico Teixeira Flávio Reis | 2010 | Renal Failure2010，，9： | 1 |
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