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| 1 | Clinicopathological and molecular genetic analysis of 4 typical Chinese HNPCC families显示文摘AIM: To study the clinicopathological and molecular genetic characteristics of typical Chinese hereditary nonpolyposis cotorectal cancer (HNPCC) families.METHODS: Four typical Chinese HNPCC families were analyzed using microdissection, microsatellite instability analysis, immunostaining of hMSH2 and hMLH1 proteins and direct DNA sequencing of hMSH2 and hMLH1 genes.RESULTS: All five tumor tissues of 4 probands from the 4typical Chinese HNPCC families showed microsatellite instability at more than two loci (MSI-H or RER +phenotype). Three out of the 4 cases lost hMSH2 protein expression and the other case showed no hMLH1 protein expression. Three pathological germline mutations (2 in hMSH2 and 1 in hMLH1 ), which had not been reported previously, were identified. The same mutations were also found in otler affected members of two HNPCC families,respectively.CONCLUSION: Typical Chinese HNPCC families showed relatively frequent germline mutation of mismstch repair genes. High-level microsateliita instability and loss of expression of mismatch repair genes correlated closely with germline mutation of mismatch repair genes. Microsatellite instability analysis and immunostaining of miamstch repair gens might serve as effective screening methods before direct DNA sequencing. It is necessary to establish clinical criteria and molecular diagnostic strategies more suitable for Chinese HNPCC families. | Qi Cai~1 Meng-Hong Sun~1 Hong-Fen Lu~1 Tai-Ming Zhang~1 Shan-Jing Mo~2 Ye Xu~2 San-Jun Cai~2 Xiong-Zeng Zhu~1 Da-Ren Shi~1 1 Department of Pathology2 Department of Abdominal Surgery,Cancer Hospital/Cancer Institute,Fudan University,Shanghai 200032,China | 2001 | World Journal of Gastroenterology2001，7，6： | 10 |
| 2 | Germline mutation analysis of hPMS2 gene in Chinese families with hereditary nonpolyposis colorectal cancer显示文摘AIM: To study the germline mutation of hPMS2 gene in 26 unrelated Chinese hereditary nonpolyposis colorectal cancer (HNPCC) probands and to fulfill the screening strategy for HNPCC in Chinese. METHODS: Genomic DNA was extracted from the peripheral blood. To avoid the interference of pseudogene in detection of the remaining 11 exons (exon 1-5, 9, 11-15), long-range polymerase chain reaction (PCR) was conducted to amplify the complete coding region of hPMS2 gene firstly. Then 1/8 of the PCR productswere used as template to amplify the individual exon respectively and DNA sequencing was done. Direct DNA sequencing of the conventional PCR products of exon 6, 7, 8 and 10 of hPMS2 gene was performed. The same analysis was made in 130 healthy persons without family histories of HNPCC to further investigate the pathological effects of the detected missense mutation. RESULTS: One HNPCC proband fulf illed Bethesda guidelines and was found to carry the germline mutation of hPMS2 gene, which has not been reported in Chinese HNPCC families. It was a missense mutation at c.1532C>T of exon 11. It was detected in three controls as well with an occurrence rate of 2.3% (3/130). Since it could not be found in the PMS2-single nucleotide polymorphism (SNP) database, this missense mutation is a new SNP unreported up to date. Meanwhile, 260 reported SNPs of hPMS2 gene were detected in the 26 HNPCC probands. The 2nd and 5th exons were probably the hot SNP regions of hPMS2 gene in Chinese HNPCC families involving 53.1% of all reported SNP. CONCLUSION: The germline mutation of hPMS2 gene may be rare in Chinese HNPCC families. The 2nd and 5th exons are hot SNP regions of hPMS2 gene. | Xia Sheng, Xiao-Yan Zhou, Xiang Du, Tai-Ming Zhang, WeiQi Sheng, Da-Ren Shi, Department of Pathology, Shanghai Cancer Center, Fudan University, Shanghai 200032, China Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China Heng-Hua Zhou, Department of Pathology, Shanghai Ninth People’s Hospital Affi liated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China San-Jun Cai, Department of Abdominal Surgery, Cancer Center, Fudan University, Shanghai 200032, China | 2010 | World Journal of Gastroenterology2010，16，30： | 6 |
| 3 | Overexpression of sterol carrier protein-2 mRNA in patients with cholesterol gallstones显示文摘BACKGROUND: Hypersecretion of biliary cholesterol is believed to be one of the important causes of lithogenic bile. Sterol carrier protein-2 ( SCP2 ) participates in choles- terol trafficking and metabolism and may play a key role in cholesterol gallstone formation. This study was undertaken to investigate the expression of liver SCP2 mRNA in pa- tients with cholesterol gallstone and those patients with non-cholesterol gallstone. METHODS: The expression of liver SCP2 mRNA was studi- ed in 36 patients with cholesterol gallstone and 30 patients with non-cholesterol gallstone by reverse transcription-poly- merase chain reaction (RT-PCR). RESULT: The expression of SCP2 mRNA was increased more significantly in patients with cholesterol gallstone than in patients with non-cholesterol gallstone. CONCLUSION: The SCP2 gene was overexpressed in pa- tients with cholesterol gallstone, indicating that SCP2 may be one of the important causes of cholesterol gallstone. | Nai-Qiang Cui, Shu-Kun Zhang, Yun-Feng Cui, Dong-Hua Li,Chang Chen and Xian-Zhong Wu Tianjin, China Institute of Acute Abdominal Diseases and Department of Surgery | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，1： | 5 |
| 4 | Effect of extended radical resection for rectal cancer显示文摘AIM: To discuss the rationality of extended radical resection (ERR) and to guide the surgical treatment of rectal cancer.METHODS: Total 211 patients who underwent ERR from 1981 to 1987 (follow-up rate of 94.8 %) were selected to study the patterns of lymphatic metastasis and therapeutic effect. The control group was made of 293 patients with rectal cancer who underwent conventional radical resection (CRR) and its follow-up rate was 98.5 %. The lymph node specimens, obtained by the triple-approach lymph node resection during the radical resection of rectal cancer, were studied by conventional pathological method.The extended radical resection, guided by the patterns of lymphatic metastasis, was applied in the clinical practice.RESULTS: The incidence of lymphatic metastasis in Chinese patients with advanced rectal cancer was 43.6 %,and that of the upper 2nd and 3rd groups and the lateral group was 14.2%, 10.9% and 11% respectively. The 5,10-year-survival rates of the ERR were 68.0 % and 47.0 %,respectively, which were much higher than those of the conventional radical resection (42.9 % and 25.3 %).CONCLUSION: The ERR for rectal cancer removes all the lymph nodes, prevents possible metastasis and finally improves the survival rate. | Xing-Shu Dong Hai-Tao Xu Zhi-Wei Yu Ming Liu Bin-Bin Cui Peng Zhao Xi-Shan Wang Department of Abdominal Surgery,the Third Affiliated Hospital,Harbin Medical University,Harbin 150040,Heilongjiang Province,China | 2003 | World Journal of Gastroenterology2003，9，5： | 3 |
| 5 | Split liver transplantation: a reliable approach to expand donor pool显示文摘Orthotopic liver transplantation as a successful treatment of end-stage liver disease is hampered by a persistent lack of cadaveric organs. Split liver transplantation, which was first successfully performed by Medical School of Hannover in 1988, has become a mature surgical technique to expand the donor pool. Between 1993 and 1999, split liver transplantation activities have increased in Europe from 1. 2% to 10. 4% in all performed liver transplantations. Current data have strongly supported that the survival rate of patients after split liver transplantation is not significantly different from that of patients after whole-size orthotopic liver transplantation. The most important step of donor graft selection is surgeon’ s observation judged by the experience of individual transplant center. The paper aims to provide the guideline of donor selection, hepatic graft splitting, and recipient management as well. DATA SOURCES: Medical School of Hannover has accumulated plentiful experience of split liver transplantation for more than 10 cases ever since 1998. Besides that, we also reviewed a variety of literatures from other famous European and American centers specialized in this field for many years. RESULTS:According to our experience combined with the view points of others, the donor should meet the following criteria as well: (1) age less than 50 years; (2) hemodynamics stable; (3) ICU less than 5 days; (4) Na less than 170 mmol/L or better if less than 150 mmol/L. In 1996 and 1997, the Hamburg group and the UCLA group separately introduced a breakthrough technique performing split liver transplantation in situ. Evidently, the in situ technique has been limited by prolonged time of donor organ procurement , coordination with other organ procurement teams, and even extra burden on donor hospital. Some groups, therefore, have restored the ex situ or bench splitting technique , and fortunately the transplant outcomes of the ex situ technique are equivalent to those of the in situ one. Recently some new techniques have been introduced to split the liver for two adult patients, including the split-cava technique. CONCLUSIONS:It is clear that the most important factor for determining the prognosis of the patient is the time of receiving liver transplantation, not the type of liver transplantation. We still need to pay close attention to the graft to recipient weight ratio (GRWR) and the UNOS classification or MELD score before the patient is subjected to split liver transplantation. | Ji-Qi Yan, Thomas Becker, Cheng-Hong Peng, Hong-Wei Li and Juergen Klempnauer Department of Surgery, Ruijin Hospital Affiliated to Shanghai Second Medical University, Shanghai 200025, China ( Yan JQ, Peng CH and Li HW) Department of Abdominal and Transplant Surgery, Medical School of Hannover, Carl-Neuberg-Str. 1 , 30625 Hannover, Germany | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，3： | 3 |
| 6 | Treatment of established colon carcinoma-bearing mice by dendritic cells pulsed with lysates of heat-treated tumor cells显示文摘To investigate the therapeutic effect of dendritic cells pulsed with lysates of heat-treated CT26 colon carcinoma cells. Bone marrow-derived DCs were pulsed with lysates of heat-treated tumor cells and were used to immunize BALB/c mice with established colon carcinoma. Cytotoxic T lymphocyte (CTL) response was detected. The therapeutic effect induced by DCs was observed by tumor weight and survival time. DCs pulsed with lysates of heat-treated tumor cells markedly induced specific cytotoxic activity of CTLs. Tumor growth in the immunized BALB/c mice was significantly inhibited and the survival time of the tumor-bearing mice was prolonged. DCs pulsed with lysates of heat-treated tumor cells have an observable therapeutic effect on established colon carcinoma-bearing mice. | YING MinGang1,ZHEN QiuHong2,LIU Sheng1,GONG FuSheng2 & XIE YunQing2 1 Abdominal Surgery,Fujian Provincial Tumor Hospital,Teaching Hospital of Fujian Medical University,Fuzhou 350014,China 2 Surgical Laboratory,Fujian Provincial Tumor Hospital,Teaching Hospital of Fujian Medical University,Fuzhou 350014,China | 2009 | Science China(Life Sciences)2009，52，9： | 2 |
| 7 | Percutaneous radiofrequency ablation approach through the spleen:initial case report for pancreatic tail gastrinoma显示文摘Gastrinoma has a low incidence, and the pancreas-originated gastrinoma is rare.Pancreatic gastrinoma patients with liver metastases have poor prognosis and short survival.Local treatment to reduce the tumor burden helps to improve symptoms and slows down tumor progression for patients with unresectable tumors.We report a case of pancreatic tail gastrinoma with unresectable liver metastases.The patient received a comprehensive minimally invasive interventional treatment, that is, chemoembolization and radiofrequency ablation for liver metastases, and percutaneous transplenic radiofrequency ablation combined with radioactive 125I seed implantation for pancreatic tail gastrinoma.The patient was followed up for more than 20 months, and showed no clear evidence of tumor recurrence.We explored the safety and feasibility of percutaneous transplenic radiofrequency ablation for unresectable pancreatic tail gastrinoma.This transplenic approach allow more indications for minimally invasive therapy and provides a new treatment option not only for patients with unresectable pancreatic tail tumor but also for patients refusing surgery. | Pei-Hong Wu1,2, Chang-Chuan Pan1,2, Zi-Lin Huang1,2, Wang Li1,2, Ming Zhao1,2, Zhi-Wei Zhou1,3 1 State Key Laboratory of Oncology in South China, Guangzhou, Guangdong 510060, P.R.China 2 Department of Medical Imaging & Interventional Radiology, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, P.R.China 3 Department of Abdominal Oncology, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong 510060, P.R.China | 2010 | Chinese Journal of Cancer2010，29，9： | 2 |
| 8 | Chronic abdominal pain in children显示文摘 | American Academy of Pediatrics Subcommittee on Chronic Abdominal Pain NorthAmerican Society forPediatricGastmenterologyHepatoloty and Nutrition | 2005 | Pediatrics2005，115，3： | 1 |
| 9 | Chronic abdominal pain in children 显示文摘 | American Academy of Pediatrics Subcommittee on Chronic Abdominal Pain North American Society for Pediatric Gastroenterology Hepatoloty and Nutrition | 2005 | Pediatrics2005，115，3： | 1 |
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| 17 | 2009 World Society of the Abdominal Compartment Syndrome显示文摘 | World Society of the Abdominal Compartment Syndrome | | 0，，11： | 1 |
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| 19 | Intraperitoneal local surgery-a systematic review 显示文摘 | Kahokehr A Sammour T anaesthetic in abdominal Soop M | 2011 | Anz JSurg2011，81，4： | 1 |
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