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| 1 | Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells显示文摘AIM: To study the therapeutic effect of three tubeguides with electrical conductivity associated to mesenchymal stem cells(MSCs) on neuro-muscular regeneration after neurotmesis.METHODS: Rats with 10-mm gap nerve injury were tested using polyvinyl alcohol(PVA), PVA-carbon nanotubes(CNTs) and MSCs, and PVA-polypyrrole(PPy). The regenerated nerves and tibialis anterior muscles were processed for stereological studies after 20 wk. The functional recovery was assessed serially for gait biomechanical analysis, by extensor postural thrust, sciatic functional index and static sciatic functionalindex(SSI), and by withdrawal reflex latency(WRL). In vitro studies included cytocompatibility, flow cytometry, reverse transcriptase polymerase chain reaction and karyotype analysis of the MSCs. Histopathology of lung, liver, kidneys, and regional lymph nodes ensured the biomaterials biocompatibility. RESULTS: SSI remained negative throughout and independently from treatment. Differences between treted groups in the severity of changes in WRL existed, showing a faster regeneration for PVA-CNTs-MSCs(P < 0.05). At toe-off, less acute ankle joint angles were seen for PVA-CNTs-MSCs group(P = 0.051) suggesting improved ankle muscles function during the push off phase of the gait cycle. In PVA-PPy and PVA-CNTs groups, there was a 25% and 42% increase of average fiber area and a 13% and 21% increase of the 'minimal Feret's diameter' respectively. Stereological analysis disclosed a significantly(P < 0.05) increased myelin thickness(M), ratio myelin thickness/axon diameter(M/d) and ratio axon diameter/fiber diameter(d/D; g-ratio) in PVA-CNT-MSCs group(P < 0.05). CONCLUSION: Results revealed that treatment with MSCs and PVA-CNTs tube-guides induced better nerve fiber regeneration. Functional and kinematics analysis revealed positive synergistic effects brought by MSCs and PVA-CNTs. The PVA-CNTs and PVA-PPy are promising scaffolds with electric conductive properties, bio- and cytocompatible that might prevent the secondary neurogenic muscular atrophy by improving the reestablishment of the neuro-muscular junction. | Jorge Ribeiro Tiago Pereira Ana Rita Caseiro Paulo Armada-da-Silva Isabel Pires Justina Prada Irina Amorim Sandra Amado Miguel Franca Carolina Goncalves Maria Ascensao Lopes Jose Domingos Santos Dina Morais Silva Stefano Geuna Ana Lúcia Luís Ana Colette Maurício | 2015 | World Journal of Stem Cells2015，7，6： | 2 |
| 2 | N-acetyl-L-cysteine combined with mesalamine in the treatment of ulcerative colitis: Randomized,placebo-controlled pilot study显示文摘AIM: To evaluate the effectiveness and safety of oral N-acetyl-L-cysteine (NAC) co-administration with mesalamine in ulcerative colitis (UC) patients. METHODS: Thirty seven patients with mild to moderate UC were randomized to receive a four-wk course of oral mesalamine (2.4 g/d) plus N-acetyl-L-cysteine (0.8 g/d) (group A) or mesalamine plus placebo (group B). Patients were monitored using the Modified Truelove-Witts Severity Index (MTWSI). The primary endpoint was clinical remission (MTWSI ≤ 2) at 4 wk. Secondary endpoints were clinical response (defined as a reduction from baseline in the MTWSI of ≥ 2 points) and drug safety. The serum TNF-α, interleukin-6, interleukin-8 and MCP-1 were evaluated at baseline and at 4 wk of treatment. RESULTS: Analysis per-protocol criteria showed clinical remission rates of 63% and 50% after 4 wk treatment with mesalamine plus N-acetyl-L-cysteine (group A) and mesalamine plus placebo (group B) respectively (OR = 1.71; 95% CI: 0.46 to 6.36; P = 0.19; NNT = 7.7). Analysis of variance (ANOVA) of data indicated a significant reduction of MTWSI in group A (P = 0.046) with respect to basal condition without significant changes in the group B (P = 0.735) during treatment. Clinical responses were 66% (group A) vs 44% (group B) after 4 wk of treatment (OR = 2.5; 95% CI: 0.64 to 9.65; P = 0.11; NNT = 4.5). Clinical improvement in group A correlated with a decrease of IL-8 and MCP-1. Rates of adverse events did not differ significantly between both groups. CONCLUSION: In group A (oral NAC combined with mesalamine) contrarily to group B (mesalamine alone), the clinical improvement correlates with a decrease of chemokines such as MCP-1 and IL-8. NAC addition not produced any side effects. | Luis G Guijarro Jose Mate Javier P Gisbert Jose Luis Perez-Calle Ignacio Marín-Jimenez Encarna Arriaza Tomás Olleros Mario Delgado Maria S Castillejo David Prieto-Merino Venancio Gonzalez Lara Amado Salvador Pea | 2008 | World Journal of Gastroenterology2008，14，18： | 2 |
| 3 | Inhibitory action of aqueous coffee ground extracts on the corrosion of carbon steel in HCl solution显示文摘 | Vanessa Vasconcelos Torres Roberto Salgado Amado Camila Faia de Sá Tatiana Lopez Fernandez Carlos Alberto da Silva Riehl Alexandre Guedes Torres Eliane D’Elia | 2011 | Corrosion Science2011，，7： | 1 |
| 4 | Long-termfunctional and morphological assessment of a stand-ardized rat sciatic nerve crush injury with a non-ser-rated clamp显示文摘 | Luis AL Amado S Geuna S | 2007 | Neurosci Methods2007，163，1： | 1 |
| 5 | Inhibitory action of aqueous coffee ground extracts on the corrosion of carbon steel in HC1 solution 显示文摘 | TORRESS V V AMADO R S de sAC F FERNANDEZ T L da SILVA RIEHI C A TORRES A G D'ELIA E | 2011 | Corrosion Science2011，53，： | 1 |
| 6 | Isolation of low-molecular-weight taste peptides from Vacherin Mont d' Or cheese显示文摘 | Mojarro-Guerra S H AMADO R ARRIGONI E | 1991 | Journal of Food Science1991，，4： | 1 |
| 7 | Guidelines for the use of an insulin infusion for the management of hyperglycemia in critically ill patients显示文摘 | Judith Jacobi Nicholas Bircher James Krinsley Michael Agus Susan S. Braithwaite Clifford Deutschman Amado X. Freire Douglas Geehan Benjamin Kohl Stanley A. Nasraway Mark Rigby Karen Sands Lynn Schallom Beth Taylor Guillermo Umpierrez John Mazuski Holger S | 2012 | Critical Care Medicine2012，，12： | 1 |
| 8 | Memory - guided Saccade Abnormalities in Schizophrenic Patients and Their Healthy, Full Biological Siblings显示文摘 | Landgraf S Amado I Borudel MC | 2008 | Psychological Medicine2008，38，6： | 1 |
| 9 | Nitrigen availability as affected by ten years of cover crop and tillage system in Southern Brazil显示文摘 | Amado T J C Femandez S B Mielniczuk J | 1998 | J Soil and water Cons1998，53，3： | 1 |
| 10 | Efficiency ofmechanical and biological chiseling in the improvement ofphysical attributes of a heavy clay Oxisol and the increment ofsoybean yield 显示文摘 | Nicoloso R S Amado T J C Schneider S | 2008 | Revista Brasileira de Gi^ncia do Solo2008，32，4： | 1 |
| 11 | Xanthine oxidase inhibition ameliorates cardiovascular dysfunction in dogs with pacing-induced heart failure显示文摘 | Amado LC Saliaris AP Raju SV Lehrke S St John M Xie J | 2005 | J Mol Cell Cardiol2005，39，： | 1 |
| 12 | Structure, e:ression profile and subceIlular localisation of four different sucrose synthase genes from barley显示文摘 | Sicilia C B Amado S H Melendi G P et ol | 2011 | Planta2011，234，： | 1 |
| 13 | Accurate and ob- jective infarct sizing bycontrast-enhanced magnetic reso- nance imaging in a canine myocardialinfaretion model 显示文摘 | Amado L Gerber B Gupta S etal | 2004 | J Am Coil Cardiol2004，44，12： | 1 |
| 14 | Challenges for performance assessment and improvement in primary health care: The case of the Potluguese health eentres显示文摘 | Amado C A Santos S P | 2009 | Health Poliey2009，91，1： | 1 |
| 15 | Accurate and objective infarct sizing by contrast-enhanced magnetic resonance imaging in a canine myocardial infarction model显示文摘 | AMADO L C GERBER B L GUPTA S N | 2004 | J Am Coll Cardiol2004，44，12： | 1 |
| 16 | Validation of a self-administered 24-hoar recall questionnaire used in large-scale dietary survey显示文摘 | BEER-BORST SS AMADO S | | 0，，03： | 1 |
| 17 | Harmful Effect of Preformed Anti-MICA Antibodies on Renal Allograft Evolution in Early Posttransplantation Period显示文摘 | Elena Sánchez-Zapardiel María J. Castro-Panete Marcela Castillo-Rama Pablo Morales David Lora-Pablos Diana Valero-Hervás Raquel Ruiz-García Jacqueline Apaza Paloma Talayero Amado Andrés José M. Morales Estela Paz-Artal | 2013 | Transplantation Journal2013，，1： | 1 |
| 18 | Validation of a self - administered 24 - hour recall questionnaire used in large -scale dietary survey 显示文摘 | Beer - Borst S Amado R | 1995 | Steinkopff Verlag1995，34，2： | 1 |
| 19 | Integrating the data envelopment analysis and the balanced scorecard approaches for enhanced performance assessment显示文摘 | AMADO A F SANTOS S P MARQUES P M | 2012 | Omega2012，，40： | 1 |
| 20 | Case report: Primary lung liposarcoma显示文摘 | Krygier G Amado A Salisbury S | 1997 | Lung Cancer1997，17，： | 1 |
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