|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Cancer stem cells and tumorigenesis显示文摘 | Pingping Zhu Zusen Fan | 2018 | Biophysics Reports2018，4，4： | 7 |
| 2 | Docking-based inverse virtual screening: methods, applications, and challenges显示文摘 | Xianjin Xu Marshal Huang Xiaoqin Zou | 2018 | Biophysics Reports2018，4，1： | 5 |
| 3 | Targeting tumor cells with antibodies enhances anti-tumor immunity显示文摘Tumor-targeting antibodies were initially defined as a group of therapeutic monoclonal antibodies (mAb)that recognize tumor-specific membrane proteins,block cell signaling,and induce tumor-killing through Fc-driven innate immune responses.However,in the past decade,ample evidence has shown that tumor-targeting mAb (TTmAb)eradicates tumor cells via activation of cytotoxic T cells (CTLs).In this review,we specifically focus on how TTmAbs induce adaptive anti-tumor immunity and its potential in combination therapy with immune cytokines,checkpoint blockade,radiation,and enzymetargeted small molecule drugs.Exploring the mechanisms of these preclinical studies and retrospective clinical data will significantly benefit the development of highly efficient and specific TTmAb-oriented anti-tumor remedies. | Zhichen Sun Yang-Xin FU Hua Peng | 2018 | Biophysics Reports2018，4，5： | 4 |
| 4 | Omentin, a novel adipokine, induces vasodilation in rat isolated blood vessels显示文摘 | Hideyuki Yamawaki Naoya Tsubaki Masashi Mukohda Muneyoshi Okada Yukio Hara | 2010 | Biochemical and Biophysical Research Communications2010，，4： | 4 |
| 5 | Characterization of CD133 + hepatocellular carcinoma cells as cancer stem/progenitor cells显示文摘 | Atsushi Suetsugu Masahito Nagaki Hitomi Aoki Tsutomu Motohashi Takahiro Kunisada Hisataka Moriwaki | 2006 | Biochemical and Biophysical Research Communications2006，，4： | 4 |
| 6 | Radiolabeled cyclic RGD peptides as radiotracers for tumor imaging显示文摘 | Jiyun Shi Fan Wang Shuang Liu | 2016 | Biophysics Reports2016，2，1： | 4 |
| 7 | PTB/nPTB: master regulators of neuronal fate in mammals显示文摘 | Jing Hu Hao Qian Yuanchao Xue Xiang-Dong Fu | 2018 | Biophysics Reports2018，4，4： | 4 |
| 8 | Skeletal intramyocellular lipid metabolism and insulin resistance显示文摘 | Yiran Li Shimeng Xu Xuelin Zhang Zongchun Yi Simon Cichello | 2015 | Biophysics Reports2015，1，2： | 4 |
| 9 | A protocol for structured illumination microscopy with minimal reconstruction artifacts显示文摘The imaging rate of structured illumination microscopy (SIM) reached 188 Hz recently. As the exposure time decreases, the camera detects fewer virtual photons, while the noise level remains the same. As a result, the signal-to-noise ratio (SNR) decreases sharply. Furthermore, the SNR decreases further because of photobleaching and phototoxicity. This decreased quality of SIM raw data may lead to surprising artifacts with various causes, which may confuse a new user of SIM microscopy. We summarize three significant possible sources of severe artifacts in reconstructed super-resolution (SR) images. Ultrafast motion of a biological sample or an uneven illumination pattern is the most difficult to be identified. The estimated parameter could also be incorrect, leading to artifact of regular patterns. Furthermore, rec on struction with the Wiener method generates stochastic artifacts due to the amplification of noise during the deconvolution process. To deal with these problems, we have established a protocol to reconstruct ultrafast SIM raw data obtained in low SNR conditions. First, we checked the quality of the raw data with the ImageJ plugin SIMcheck before rec on struction. Then, a modified parameter estimation method was used to improve the precision of the parameters. Finally, an iterative algorithm was used for SIM reconstruction under low signal-to-noise ratio cond让ions. This procedure effectively suppressed the artifacts in the super-resoluti on images rec on structed from raw data of low signal-to-noise ratio. | Junchao Fan Xiaoshuai Huang Liuju Li Shan Tan Liangyi Chen | 2019 | Biophysics Reports2019，5，2： | 4 |
| 10 | Natural and engineered bacterial outer membrane vesicles显示文摘Bacterial outer membrane vesicle(OMV)is a kind of spherical lipid bilayer nanostructure naturally secreted by bacteria,which has diverse functions such as intracellular and extracellular communication,horizontal gene transfei;transfer of contents to host cells,and eliciting an immune response in host cells.In this review,several methods including ultracentrifugation and precipNation for isolating OMVs were summarized.The latest progresses of OMVs in biomedical fields,especially in vaccine development,cancer treatment,infection control,and bioimaging and detection were also summarized in this review.We highlighted the importance of genetic engineering for the safe and effective application and in facilitating the rapid development of OMVs.Finally,we discussed the bottleneck problems about OMVs in preparation and application at present and put forward our own suggestions about them.Some perspectives of OMVs in biomedical field were also provided. | Guangchao Qing Ningqiang Gong Xiaohui Chen Jing Chen Hong Zhang Yongchao Wang Ruifang Wang Shouwen Zhang Zhen Zhang Xianxian Zhao Yang Luo Xing-Jie Liang | 2019 | Biophysics Reports2019，5，4： | 4 |
| 11 | Detection of Loop-Mediated Isothermal Amplification Reaction by Turbidity Derived from Magnesium Pyrophosphate Formation显示文摘 | Yasuyoshi Mori Kentaro Nagamine Norihiro Tomita Tsugunori Notomi | 2001 | Biochemical and Biophysical Research Communications2001，，1： | 3 |
| 12 | The long non-coding RNA H19-derived miR-675 modulates human gastric cancer cell proliferation by targeting tumor suppressor RUNX1显示文摘 | Ming Zhuang Wen Gao Jing Xu Ping Wang Yongqian Shu | 2013 | Biochemical and Biophysical Research Communications2013，，： | 3 |
| 13 | Solubilization and purification of recombinant modified C-reactive protein from inclusion bodies using reversible anhydride modification显示文摘 | Lawrence A. Potempa Zhen-Yu Yao Shang-Rong Ji Janos G. Filep Yi Wu | 2015 | Biophysics Reports2015，1，1： | 3 |
| 14 | Protocol for analyzing protein liquid-liquid phase separation显示文摘Numerous cellular functions occur in spatially and temporally confined regions. Recent studies have shown that membrane-less organelles and compartments in the cell are assembled via liquid-liquid phase separation (LLPS). In vitro LLPS assays using recombinant expressed and purified proteins are necessary for us to further understand how the assembly of phase-separated compartments is regulated in cells. However;uniform standards and protocols are lacking for these in vitro studies. Here, we describe a step-by-step protocol commonly used to investigate in vitro LLPS using purified proteins. This protocol includes expression and purification of the studied proteins, induction of LLPS of the purified proteins, and studies of the biophysical properties of the liquid droplets formed by LLPS. These protocols can be easily followed by researchers to investigate the LLPS behaviors of proteins of interest. | Zheng Wang Gangming Zhang Hong Zhang | 2019 | Biophysics Reports2019，5，1： | 3 |
| 15 | The Possible Role of Hydrogen Sulfide as an Endogenous Smooth Muscle Relaxant in Synergy with Nitric Oxide显示文摘 | Rumiko Hosoki Norio Matsuki Hideo Kimura | 1997 | Biochemical and Biophysical Research Communications1997，，3： | 3 |
| 16 | Co-encapsulation of curcumin and doxorubicin in albumin nanoparticles blocks the adaptive treatment tolerance of cancer cells显示文摘The adaptive treatment tolerance (ATT) of cancer cells is the main encumbrance to cancer chemotherapy. A potential solution to this problem is to treat cancer cells with multiple drugs using nanoparticles (NPs). In this study, we tested the co-administration of curcumin (Cur) and doxorubicin (Dox) to MCF-7 resistant breast cancer cells to block the ATTand elicit efficient cell killing. Drugs were co-administered to cells both sequentially and simultaneously. Sequential drug co-administration was carried out by pre-treating the cells with albumin nanoparticles (ANPs) loaded w让h Cur (Cur@ANPs) followed by treatment with Dox-loaded ANPs (Dox@ANPs). Simultaneous drug co-administration was carried out by treating the cells with ANPs loaded with both the drugs (Cur/Dox@ANPs). We found that the simultaneous drug co-administration led to a greater intra-cellular accumulation of Dox and cell killing with respect to the sequential drug co-administration. However;the simultaneous drug co-administration led to a lower intracellular accumulation of Cur with respect to the sequential drug co-administration. We showed that this result was due to the aggregation and entrapment of Cur in the lysosomes as soon as it was released from Cur@ANPs, a phenomenon called lysosomotropism. In contrast, the simultaneous release of Dox and Cur from Cur/Dox@ANPs into the lysosomes led to lysosomal pH elevation, which, in turn, avoided Cur aggregation, led to lysosome swelling and drug release in the cytosol, and finally provoked efficient cell killing. Our study shed the light on the molecular processes driving the therapeutic effects of anti-cancer drugs co-administered to cancer cells in different manners. | Seyed Mohammad Motevalli Ahmed Shaker Eltahan Lu Liu Andrea Magrini Nicola Rosato Weisheng Guo Massimo Bottini Xing-Jie Liang | 2019 | Biophysics Reports2019，5，1： | 3 |
| 17 | DNA-Binding Specificity of the ERF/AP2 Domain of Arabidopsis DREBs, Transcription Factors Involved in Dehydration- and Cold-Inducible Gene Expression显示文摘 | Yoh Sakuma Qiang Liu Joseph G. Dubouzet Hiroshi Abe Kazuo Shinozaki Kazuko Yamaguchi-Shinozaki | 2002 | Biochemical and Biophysical Research Communications2002，，3： | 3 |
| 18 | A genetically encoded ratiometric calcium sensor enables quantitative measurement of the local calcium microdomain in the endoplasmic reticulum显示文摘The local Ca^2+ release from the heterogeneously distributed endoplasmic reticulum (ER) calcium store has a critical role in calcium homeostasis and cellular function. However;single fluorescent proteinbased ER calcium probes experience challenges in quantifying the ER calcium store in differing live cells, and intensity-based measurements make it difficult to detect local calcium microdomains in the ER. Here, we developed a genetically encoded ratiometric ER calcium indicator [GCEPIA1-SNAPer] that can detect the real-time ER calcium store and local calcium microdomains in live cells. GCEPIA1-SNAPer was located in the lumen of the ER and showed a linear;reversible and rapid response to changes in the ER calcium store. The GCEPIA1-SNAPer probe effectively monitored the depletion of the ER calcium store by TG or starvation treatment, and through 让s use we identified heterogeneously distributed calcium microdomains in the ER which were correlated w让h the distribution of STIM1 clusters upon ER calcium store depletion. Lastly, GCEPIA1-SNAPer can be used to detect the ER calcium store by high-throughput flow cytometry and confers the ability to study the function of calcium microdomains of the ER. | Chen Luo Huiyu Wang Qi Liu Wenting He Lin Yuan Pingyong Xu | 2019 | Biophysics Reports2019，5，1： | 3 |
| 19 | Mitochondrial protein sulfenation during aging in the rat brain显示文摘There is accumulating evidence that cysteine sulfenation (cys-SOH) in proteins plays an important rolein cellular response to oxidative stress. The purpose of the present study was to identify mitochondrialproteins that undergo changes in cys-SOH during aging. Studies were conducted in rats when they were5 or 30 months of age. Following blocking of free protein thiols with N-ethylmaleimide, protein sulfenicacids were reduced by arsenite to free thiol groups that were subsequently labeled with biotin-maleimide. Samples were then comparatively analyzed by two-dimensional Western blots, and proteinsshowing changes in sulfenation were selectively identified by mass spectrometry peptide sequencing.As a result, five proteins were identified. Proteins showing an age-related decrease in sulfenationinclude pyruvate carboxylase and pyruvate dehydrogenase; while those showing an age-relatedincrease in sulfenation include aconitase, mitofilin, and tubulin (α-1). Results of the present studyprovide a general picture of mitochondrial protein sulfenation in brain oxidative stress and implicatethe involvement of protein sulfenation in overall decline of mitochondrial function during brain aging. | Xiaorong Yang Jinzi Wu Siqun ling Michael J. Forster Liang-Jun Yan | 2018 | Biophysics Reports2018，4，2： | 3 |
| 20 | Combining biophysical methods to analyze the disulfide bond in SH2 domain of C-terminal Src kinase显示文摘 | Dongsheng Liu David Cowburn | 2016 | Biophysics Reports2016，2，1： | 3 |
您所在的机构暂未开通此部分的功能，请联系管理员