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| 1 | Ala-Gln improves varicocele-induced testicular injury by increasing HSP70 and antioxidant activity in male rats显示文摘This worked aimed to test the hypothesis that L-alanyl-L-glutamine(Ala-Gln)improves the varicocele-induced testicular injury,which causes male infertility.For this purpose,fifty adult male Wistar rats received the varicocele(VC)surgery at the left renal vein.Biomarkers were determined 2,4,and 8 weeks after VC(n=10/each detection).Four weeks after VC,rats received Ala-Gln(1.125 g/kg)treatment with and or saline for 1 week(n=10/each group).Rats in the sham group were also detected for biomarkers at 2,4,and 8 weeks(n=10/each detection).VC caused testicular injury detected by hematoxylin–eosin(H&E)staining,immunohistochemistry,and TUNEL assay.HSP70 mRNA was detected quantitative RT-PCR,SOD,and CAT by nitroblue tetrazolium(NBT)method and 8-OHDG by ELISA.The results showed that varicocele induced injury in the testes.The weight of the left testes was lower than that of the right testes in VC-bearing rats(p<0.01).The relative numbers of sustentacular and spermatogenic cells were decreased after VC(p<0.01).In sham-4 wk,VC-4wk,VC-5wk and Ala-Gln groups,the apoptosis index was 5.10±1.14,13.22±3.63,33.62±3.56 and 22.33±2.61,relative level of HSP70 mRNA 1.00±0.12,0.53±0.05,0.51±0.04 and 1.62±0.15 fold,SOD 16.4±0.23,13.4±0.17,10.01±1.06 and 19.53±2.26 U/mg protein,CAT 2.16±0.31,1.07±0.28,and 1.31±0.26 and 3.46±0.71 U/mg,8-OHDG 5.23±0.67,6.81±0.78,7.16±1.22 and 4.14±0.73 pg/μg DNA,respectively(p<0.01).Our results suggest that Ala-Gln prevented the VC-induced testicular injury.We have firstly reported that Ala-Gln protects against varicocele-induced testicular injuries by up-regulation of HSP70 and antioxidants,SOD and CAT,and down-regulation of oxidant 8-OHDG,resulting in reducing apoptosis in the testis. | LEI YU ZHEN FAN HONGQIANG WANG WENXIAN LI TAO JING QIANG LI SHENQIAN LI PEITAO WANG XUJUN XUAN | 2021 | BIOCELL2021，45，2： | 3 |
| 2 | Mechanisms involved in the cytotoxic effects of berberine on human colon cancer HCT-8 cells显示文摘Berberine,a constituent of some traditional Chinese medicinal plants,has been reported to have cytotoxicity effects on different human cancer cell lines.There is no available information about the effects and mechanism of action of berberine on human colon cancer cell line HCT-8.In this paper,the cytotoxicity of berberine on HCT-8 cancer cells was investigated by MTT assay,fluorescence microscopy and flow cytometry analysis.Our data revealed that berberine could significantly inhibit the growth of HCT-8 cells in a dose-and time-dependent manner.Morphology of apoptotic cells was studied with acridine orange/ethidium bromide staining.The concentrations of lactate dehydrogenase and both acid and alkaline phos-phatases were significantly increased in cell supernatants after berberine treatment,suggesting cell death.Furthermore,flow cytometry analysis showed that berberine could arrest HCT-8 cells at S phase in a time-dependent manner.To further investigate the apoptotic molecular mechanism,reverse transcription-poly-merase chain reaction(RT-PCR)and western blotting methods were used.The up-regulated mRNA and/or protein expressions of Fas,FasL,TNF-α,caspase-3 and down-regulation of pro-caspase-3 suggest that the death receptor pathway may be involved in the apoptotic pathway induced by berberine.Decrease of Bcl-2 and increase of Bax in mRNA and/or protein expressions showed that the Bcl-2 family proteins were involved in berberine-induced apoptosis.We also found that berberine-induced apoptosis was associated with an up-regulated expressions of p53 and prohibitin(PHB),and decreased vimentin expression.These results suggest that berberine can suppress cell growth and reduce cell survival by arresting the cell-cycle and by inducing apoptosis of HCT-8 cells. | LI-NA XU BI-NAN LU MING-MING HU YOU-WEI XU XU HAN YAN QI JIN-YONG PENG | 2012 | BIOCELL2012，36，3： | 3 |
| 3 | Nerve growth factor alleviates cerebral infarction and neurologic deficits by regulating VEGF,SDF-1 and S100A12 expression through PI3K pathway显示文摘Stroke remains the leading cause of death and disability worldwide,which destroys the quality of patients’lives and thus is becoming a heavy burden to the society.However,the current therapeutic approaches are far from satisfaction.The objective of this study is to elucidate the impact of nerve growth factor(NGF)on the brain damage induced by cerebral ischemia and its potential molecular mechanism.Middle cerebral artery occlusion(MCAO)rats were used as animal models and neurological functions were evaluated by modified Neurological Severity Score(NSS).Brain cell apoptosis was analyzed by TUNEL-positive staining while brain infarct size was determined according to 2% 2,3,5-triphenyltetrazolium chloride(TCC)staining volume.Rats receiving NGF demonstrated significantly alleviated brain damage,reflected by a substantial improvement in the neurobehavioral outcome,a decrease in brain cell apoptosis and shrinkage of brain infarct volume.Further analysis revealed a markedly elevated circulating vascular endothelial growth factor(VEGF)and stromal cell-derived factor 1(SDF-1)levels as well as a significant downregulation of SA10012 expression in NGF treated group compared with the untreated group.Strikingly,the protective effect of NGF on cerebral ischemic injury was abolished in rats treated with both NGF and PI3K inhibitors,indicating that phosphoinositide-3-kinase(PI3K)signaling is essential for NGF function.In conclusion,NGF treatment might be a potential therapeutic approach against cerebral infarction by downregulating SA10012 expression and upregulating VEGF,SDF-1 in a PI3K signaling dependent manner. | ZHENG LI ZHONGLE ZHAO PENG ZHANG | 2019 | BIOCELL2019，43，3： | 3 |
| 4 | Implications of enolase in the RANKL-mediated osteoclast activity following spinal cord injury显示文摘Spinal Cord Injury(SCI)is a debilitating condition characterized by damage to the spinal cord,resulting in loss of function,mobility,and sensation.Although increasingly prevalent in the US,no FDA-approved therapy exists due to the unfortunate complexity of the condition,and the difficulties of SCI may be furthered by the development of SCI-related complications,such as osteoporosis.SCI demonstrates two crucial stages for consideration:the primary stage and the secondary stage.While the primary stage is suggested to be immediate and irreversible,the secondary stage is proposed as a promising window of opportunity for therapeutic intervention.Enolase,a metabolic enzyme upregulated after SCI,performs non-glycolytic functions,promoting inflammatory events via extracellular degradative actions and increased production of inflammatory cytokines and chemokines.Neuron-specific enolase(NSE)serves as a biomarker of functional damage to neurons following SCI,and the inhibition of NSE has been demonstrated to reduce signs of secondary injury of SCI and to ameliorate dysfunction.This Viewpoint article involves enolase activation in the regulation of RANK-RANKL pathway and summarizes succinctly the mechanisms influencing osteoclast-mediated resorption of bone in SCI.Our laboratory proposes that inhibition of enolase activation may reduce SCI-induced inflammatory response and decrease osteoclast activity,limiting the chances of skeletal tissue loss in SCI. | RAMSHA SHAMS NAREN LBANIK AZIZUL HAQUE | 2021 | BIOCELL2021，45，6： | 3 |
| 5 | Mycorrhiza improves plant growth and photosynthetic characteristics of tea plants in response to drought stress显示文摘Tea plants are sensitive to soil moisture deficit,with the level of soil water being a critical factor affecting their growth and quality.Arbuscular mycorrhizal fungi(AMF)can improve water and nutrient absorption,but it is not clear whether AMF can improve the photosynthetic characteristics of tea plants.A potted study was conducted to determine the effects of Claroideoglomus etunicatum on plant growth,leaf water status,pigment content,gas exchange,and chlorophyll fluorescence parameters in Camellia sinensis cv.Fuding Dabaicha under well-watered(WW)and drought stress(DS)conditions.Root mycorrhizal colonization and soil hyphal length were significantly reduced by the eightweek DS treatment.AMF inoculation displayed a significant increase in shoot and root biomass production.The relative water content,leaf water potential,nitrogen balance index,pigment content,maximum photometric effect(Fv/Fm,QY_max),and steady-state photometric effect Y(II)(QY_Lss)decreased dramatically,while the leaf water saturation deficit and steady-state non-photochemical fluorescence quenching(NPQ_Lss)generally increased under DS conditions.Mycorrhizal treatment induced significantly higher relative water content,leaf water potential,nitrogen balance index,pigment(chlorophyll,flavonoid,and anthocyanin)content,net photosynthesis rate,transpiration rate,stomatal conductance,intercellular CO_(2)concentration,QY_max,and QY_Lss;however,it resulted in a lower leaf water saturation deficit and NPQ_Lss under both WW and DS conditions,as compared with nonmycorrhizal plants.These results imply that AMF promoted tea plant growth and alleviated negative effects of DS by promoting gas exchange,regulating the water status of leaves,and regulating photosynthetic parameters. | FENGJUN DAI ZIYI RONG QIANGSHENG WU ELSAYED FATHI ABD_ALLAH CHUNYAN LIU SHENGRUI LIU | 2022 | BIOCELL2022，46，5： | 2 |
| 6 | Meta-analysis evaluation of the treatment of neonatal hypoxic-ischemic encephalopathy with ganglioside显示文摘The efficacy and safety of ganglioside in the treatment of neonates who suffer from hypoxic-ischemic encephalopathy(HIE)needs to be fully evaluated.We searched the following databases:PubMed,ScienceDirect,LISTA,CNKI,Chinese biomedical literature database and Wanfang digital journals of full-text database to determine the inclusion and exclusion criteria of papers and a total of 12 papers were included after quality evaluation.Then we conducted the meta-analysis with RevMan5.0 software.The results showed that compared with the control group,the abnormal rate declined in the ganglioside-treated group(relative risk(RR)=0.27,95%confidence interval(CI)=0.05-1.96).NBNA records of the 7,10-14d neonates were improved effectively:RR(95%CI)were 2.28(0.86-3.42)and 2.53(1.04-2.92)respectively.Neural system sequelae incidence was reduced significantly:RR(95%CI)=0.35:(0.15-0.79).Ganglioside treatment could effectively reduce the abnormality rate of head size,improve the neurological score,reduce the incidence of neurological sequelae,and significantly prompt clinical recovery for neonates with HIE. | Wen LI Junli YANG Dong ZHOU Jinghui ZHANG Qingcui ZHUO | 2019 | BIOCELL2019，43，1： | 2 |
| 7 | Butein imparts free radical scavenging, anti-oxidative and proapoptotic properties in the flower extracts of Butea monosperma显示文摘The flower of Butea monosperma(Lam.)(Fabaceae)has been used in traditional Indian medicine in the treatment of many ailments including liver disorders.To understand the pharmacological basis of its beneficial effects,the extracts of dried flowers in water,methanol,butanol,ethyl acetate and acetone were evaluated for free radical scavenging and pro-apoptotic activities in cell cultures(human hepatoma Huh-7 cell line and immortalized AML-12 mouse hepatocytes).Butrin and butein-the active constituents of flower extracts-were used as reference molecules.The levels of cell injury markers like lactate dehydrogenase,glutathione and lipid peroxidation and primary antioxidant enzymes glutathione S-transferase and catalase were also measured.The aqueous and butanolic extracts exhibited better 2,2-diphenyl-1-picrylhydrazyl scavenging and cytotoxic activities in hepatoma cells than in immortalized hepatocytes.Interestingly,butein inhibited 2,2-diphenyl-1-picrylhydrazyl radical better than butrin.The aqueous and butanolic extracts were further investigated for hepatoprotection against carbon tertrachloride-induced biochemical changes and cell death.Both extracts,just as butrin and butein,significantly reversed the cellular glutathione levels and lipid peroxidation,and glutathione–S-transferase activity.Lactate dehydrogenase leakage and cell death were also prevented.However,only butein revived the catalase activity.Thus,the butein content of Butea monosperma flower extracts is important for free radical scavenging activity,apoptotic cell death and protection against oxidative injury in hepatic cells. | ANURADHA SEHRAWAT VIJAY KUMAR | 2012 | BIOCELL2012，36，2： | 2 |
| 8 | Microfluidic chips for the endothelial biomechanics and mechanobiology of the vascular system显示文摘Endothelial cells arranged on the vessel lumen are constantly stimulated by blood flow,blood pressure and pressureinduced cyclic stretch.These stimuli are sensed through mechanical sensory structures and converted into a series of functional responses through mechanotransduction pathways.The process will eventually affect vascular health.Therefore,there has been an urgent need to establish in vitro endothelial biomechanics and mechanobiology of models,which reproduce three-dimensional structure vascular system.In recent years,the rapid development in microfluidic technology makes it possible to replicate the key structural and functionally biomechanical characteristics of vessels.Here,we summarized the progress of microfluidic chips used for the investigation of endothelial biomechanics and mechanobiology of the vascular system.Firstly,we elucidated the contribution of shear stress and circumferential stress,to vascular physiology.Then,we reviewed some applications using microfluidic technology in angiogenesis and vasculogenesis,endothelial permeability and mechanotransduction,as well as the blood-brain barrier under these physical forces.Finally,we discussed the future obstacles in terms of the development and application of microfluidic vascular chips. | HAORAN SU KEXIN LI XIAO LIU JING DU LI WANG XIAOYAN DENG YUBO FAN | 2021 | BIOCELL2021，45，4： | 2 |
| 9 | Histological analysis of pollen-pistil interactions in sour passion fruit plants (Passiflora edulis Sims)显示文摘The success of sexual plant reproduction is directly influenced by specific interactions between the pollen and pistil.Light,fluorescence and scanning electron microscopy techniques were used to evaluate the steps of pollination in sour passion fruit plants(Passiflora edulis Sims).In the compatible interaction,pollen tubes grow through stigma projections towards the ovary.The pollen grain surface was found to be spheroidal and to consist of heteroreticulate exine with six colpi.Furthermore,analysis in vivo of pollenpistil interactions indicated that stigmas of flowers 24 hours before anthesis are unable to discriminate compatible(genetically unrelated)and incompatible(genetically related)pollen grains.Taken together,these results provide insight into the cellular mechanisms underlying pollination in passion fruit plants. | HÉRIKA CHAGAS MADUREIRA TELMA NAIR SANTANA PEREIRA MAURA DA CUNHA DENISE ESPELLET KLEIN | 2012 | BIOCELL2012，36，2： | 2 |
| 10 | Transcriptome analysis of purple pigment formation in Colocasia esculenta显示文摘Taro(Colocasia esculenta(L.)Schott)is an important crop in Africa,Southeast Asia,and subtropics and is used as a food and medicine.The purple color pigmentation is an appealing character in taro.We sampled taro corms of the cultivar‘Lipu Taro’at four developmental stages,including LPYS1(without purple pigment,50 days of development(DOD)),LPYS2(very few purple pigments,75 DOD),LPYS3(moderate purple pigments,115 DOD)and LPYS4(high purple pigments,205 DOD).The purpose of our study was to identify the key genes underpinning the purple pigmentation in taro based on RNA-sequencing.Through RNA-Seq,6453 differentially expressed transcripts(DETs)were identified between purple and non-purple pigmented samples.We identified 41 and 12 flavonoid and anthocyanin related DETs transcripts,respectively.These DETs were upregulated at LPYS2,LPYS3,and LPYS4 as compared to LPYS1,indicating their positive contribution to the color formation in taro.Moreover,we identified several DETs encoding for transcription factors,including MYB and bHLH,known to be major regulators of structural genes involved in the flavonoid-anthocyanin pathway.Finally,we reported several plant hormones(ethylene,auxin,gibberellin,jasmonic acid,and cytokinin)related DETs,which are predicted to play important roles in the corm coloration.Different regulation of transcripts representing the flavonoid-anthocyanin biosynthesis pathway,plant hormone transduction pathway,and transcription factors may have key roles in purple pigmentation in taro.Our findings will facilitate future research on improving the quality and appeal of taro. | FANGLIAN HE WEIQING DONG SHAOLONG WEI ZUYANG QIU JINGLI HUANG HUIPING JIANG SHIYU HUANG LILI LIU | 2021 | BIOCELL2021，45，3： | 2 |
| 11 | Autophagy, apoptosis and organelle features during cell exposure to cadmiumč显示文摘Cadmium(Cd)induces several effects in different tissues,but our knowledge of the toxic effects on organelles is insuffi cient.To observe the progression of Cd effects on organelle structure and function,HuH-7 cells(human hepatic carcinoma cell line)were exposed to CdCl2 in increasing concentrations(1μM–20μM)and exposure times(2 h–24 h).During Cd treatment,the cells exhibited a progressive decrease in viability that was both time-and dose-dependent.Cd treated cells displayed progressive morphological changes that included cytoplasm retraction and nuclear condensation preceding a total loss of cell adhesion.Treatment with 10μM for 12 h led to irreversible damages.Before these drastic and irreparable damages,treated cells(5μM for 12 h)presented a progressive loss of mitochondrial function and cytoplasm acidifi cation as well as dysfunction and disorganization of microfi laments and endoplasmic reticulum.These damages led to the induction of apoptotic events and an increase in autophagic bodies in the cytoplasm.These results revealed that Cd affects multiple intra-cellular targets that induce alterations in the mitochondria,cytoskeleton,endoplasmic reticulum and acidic compartments,ultimately culminating in cell death via apoptotic and autophagic pathways. | CRISTIANE DOS SANTOS VERGILIO EDÉSIO JOSÉTENÓRIO DE MELO | 2013 | BIOCELL2013，37，2： | 2 |
| 12 | Hellebrigenin induces apoptosis in colorectal cancer Cells through induction of excessive reactive oxygen species显示文摘Traditional Chinese medicine(TCM)has been increasingly applied in both preventing and treating a variety of cancers in the last decades,attributing to its fewer side effects as compared with chemotherapy drugs.Hellebrigenin,a component of Chanpi from the skin of Bufo bufogargarizans Cantor or Duttaphrynus melanostictus has been reported to have an obvious anti-cancer activity on various cancers.However,the effect and mechanism of hellebrigenin on colorectal cancers were still unknown.Herein,the present study demonstrated hellebrigenin significantly reduced viability and triggered apoptosis via the intrinsic pathway in colorectal cancer cell lines HCT116 and HT29 in vitro and in vivo.Moreover,hellebrigenin led to a reduction of mitochondrial membrane potential.In addition,treatment with hellebrigenin could result in the induction of excessive reactive oxygen species,which led to cell apoptosis.These results indicated that hellebrigenin had anti-cancer potential in the treatment of colorectal cancers. | CHUNJIAO LIU QINHONG KONG FENG PAN SHAN JIANG LINGJIE MENG GAI HUANG LIDAN LU SANHUA LI YUN LIU | 2021 | BIOCELL2021，45，4： | 2 |
| 13 | Musk secretion in muskrats(Ondatra zibethicus L.):association with lipid and cholesterol metabolism-related pathways显示文摘Male muskrats(Ondatra zibethicus L.)secrete musk from their scent glands during musk secretion season.Musk plays an important role as a communication pheromone during the breeding season.In this study,gas chromatography–mass spectrometry(GC–MS)was used to analyze the main components of musk.The GC–MS results after methyl esterification showed that 71.55%of the musk is composed of fatty acids.The other components of muskrat musk include cholesterol(9.31%)and other organics.Transcriptome comparison between musk secretion and non-secretion seasons showed significant changes in the scent glands for 53 genes involved in fatty acid and cholesterol synthesis and metabolism regulatory pathways,which include fatty acid biosynthesis,elongation,and metabolism;steroid biosynthesis;steroid hormone biosynthesis pathways.A reverse transcription–polymerase chain reaction analysis confirmed these detected changes.Overall,our results indicated that lipid synthesis and metabolism play important roles in musk compound synthesis by providing energy for musk production,and the produced musk provides a mechanism for male muskrats to communicate with females during the breeding season. | TIANXIANG ZHANG MEISHAN ZHANG MINGHUI SHI WEIJIANG JIN SHUMIAO ZHANG MENGYUAN FAN YIMENG LI YUPING MENG XUELIN TIAN SHUQIANG LIU DEFU HU | 2021 | BIOCELL2021，45，2： | 2 |
| 14 | 3-epi-bufotalin suppresses the proliferation in colorectal cancer cells through the inhibition of the JAK1/STAT3 signaling pathway显示文摘Traditional Chinese medicine(TCM)has been increasingly employed in the last decades in China for both preventing and treating a variety of cancers.3-epi-bufotalin is an active ingredient of TCM“Chanpi”with anti-tumor potential.However,the effect and mechanism of 3-epi-bufotalin on colorectal cancers were not well disclosed.The present study demonstrated that 3-epi-bufotalin could reduce viability,trigger apoptosis,and block the cell cycle at the G2/M stage in colorectal cancer cell lines HT29,RKO,and COLO205 in vitro.Moreover,3-epi-bufotalin inhibited the JAK1/STAT3 signaling pathway.These results indicated the anti-proliferation ability of 3-epi-bufotalin in colorectal cancer cells. | SANHUA LI QINGHONG KONG XIAOKE ZHANG XINTING ZHU CHUNBO YU CHANGYAN YU NIAN JIANG JING HUI LINGJIE MENG YUN LIU | 2022 | BIOCELL2022，46，11： | 2 |
| 15 | Gene expression profile of Sox1,Sox2,p53,Bax and Nestin in neural stem cells and adult mouse brain tissues显示文摘Histone deacetylation is a key modulator involved in cell proliferation,apoptosis,and mRNA transcription.However,the effects of histone deacetylation on C17.2 neural stem cells(NSCs)remain unclear.Here,the histone deacetylase inhibitors nicotinamide and trichostatin A(TSA)were used to determine the role of histone deacetylation on gene transcription in NSCs.The results showed that the mRNA expression of p53,Sox1,Sox2,and Bax were significantly higher in E14.5 NSCs than in C17.2 NSCs.Nestin,a marker gene of neuronal differentiation,did not differ significantly between E14.5 NSCs and C17.2 NSCs.The transcription levels of p53 and Nestin were significantly higher in C17.2 NSCs than in differentiated brain tissues,and the expression of Bax,Sox1,and Sox2 was higher in the olfactory bulb than in other brain tissues.Nicotinamide and TSA treatment decreased the transcription of Sox2,p53,Nestin,and Bax in C17.2 NSCs,although the difference was statistically significant only for Sox2 and Nestin,Sox1 transcription was not detected.These results demonstrated that mRNA expression profiles differ between C17.2 NSCs,E14.5 NSCs,and adult mouse brain tissues,and HDAC inhibitors regulate gene expression by modulating histone acetylation. | HAIFENG WANG KAI WANG JUAN GUO TIEQIAO WEN | 2019 | BIOCELL2019，43，2： | 2 |
| 16 | 20(R)-ginsenoside Rg3,a product of high-efficiency thermal deglycosylation of ginsenoside Rd,exerts protective effects against scrotal heat-induced spermatogenic damage in mice显示文摘Heat stress(HS)reaction can lead to serious physiological dysfunction associated with cardiovascular and various organ diseases.Ginsenoside Rg3(G-Rg3)is a representative component of ginseng rare saponin and can protect against multiple organs,also used as functional food to adjust the balance of the human body,but the therapeutic effect and molecular mechanism of G-Rg3 on male diseases under HS are underexplored.The aim of the present study,G-Rg3 was prepared through the efficient conversion of ginsenoside Rd and investigate the contribution of G-Rg3 to testicular injury induced exposure to HS.All mice were divided into four groups as follows:normal group,HS group,and HS+G-Rg3(5 and 10 mg/kg)groups.G-Rg3 was administered orally for 14 days,then exposed to a single scrotal heat treatment(43°C,18min)on the 7th day.After HS treatment,the morphology of testis and epididymis changes,and caused a significant loss of multinucleated giant cells,desquamation of germ cells in destructive seminiferous tubules,and degenerative Leydig cells,further destroying the production of sperm.After administration G-Rg3(5 and 10 mg/kg/day)for 2 weeks,the spermatogenic-related indexes of testosterone levels and superoxide dismutase(SOD)activity,glutathione(GSH)content significantly(p<0.01)increase compared with the HS group.Moreover,G-Rg3 treatment effectively ameliorated the production of malondialdehyde(MDA)(p<0.05 or p<0.01).Importantly,G-Rg3 exhibited the protective potential against HS-induced injury not only suppressing the protein levels of heme oxygenase-1(HO-1),hypoxia-inducible factor-1α(HIF-1α),and heat shock protein 70(HSP70)but also modulating the Bcl-2 family(p<0.01 or p<0.001)and activation of mitogen-activated protein kinase(MAPK)signaling pathways(p<0.01).For most of the parameters tested,the HS+G-Rg3(10 mg/kg)group exhibited potent effects compared with those exhibited by the low dose(5 mg/kg)group.In conclusion,the present study demonstrated that G-Rg3 exerted protective effects against HS-induced testicular dysfunction via inhibiting the MAPK-mediated oxidative stress and apoptosis in mice. | WEI LIU ZI WANG JING LENG HENG WEI SHEN REN XIAOJIE GONG CHEN CHEN YINGPING WANG RUI ZHANG WEI LI | 2020 | BIOCELL2020，44，4： | 2 |
| 17 | SOD1^(G93A) Induces a Unique PSAP-Dependent Mitochondrial Apoptosis Pathway via Bax-Bak Interaction显示文摘Amyotrophic lateral syndrome(ALS)is a progressive degenerative disorder characterized by motor neuron death and axon degeneration.Mitochondrial dysfunction plays a key role in the pathogenesis of ALS,the mechanism of which remains poorly understood.The B-cell lymphoma-2(Bcl-2)family of proteins that control and mediate mitochondrial function and apoptosis,including the pro-apoptotic members Bcl2-Associated X(Bax),are involved in ALS development.The death receptor 6(DR6)regulates motor neuron death in ALS,and DR6 antibodies can prevent axon degeneration and motor neuron damage by blocking DR6.Previous studies demonstrated that PSAP localized to mitochondria and was required for DR6-induced apoptosis.In this study,SOD1^(G93A) was transfected into the motor neuron cell line NSC-34 to serve as an ALS cell model in vitro.The data assessed the role of PSAP in SOD1^(G93A)induced apoptosis and demonstrated that the overexpression of SOD1^(G93A),but not wtSOD1,induced PARP cleavage,caspase-3 activation,cytochrome c release,and Bax translocation.PSAP,Bax,and Bak were necessary for SOD1^(G93A)induced apoptosis,as silencing PSAP inhibited SOD1^(G93A)-mediated cell death that was dependent on Bax-Bak interaction. | HAN NIU XIN CHEN XUEQI FU JINGTIAN ZHANG GUODONG LI YUXIANG WANG JIAYUE SONG XUETING MA CHEN HU XUEMIN XU FUQIANG ZHANG LINLIN ZENG | 2021 | BIOCELL2021，45，4： | 2 |
| 18 | The cellular microenvironment and cytoskeletal actin dynamics in liver fibrogenesis显示文摘Hepatic stellate cells(HSCs)are the primary effector cells in liver fibrosis.In the normal liver,HSCs serve as the primary vitamin A storage cells in the body and retain a“quiescent”phenotype.However,after liver injury,they transdifferentiate to an“activated”myofibroblast-like phenotype,which is associated with dramatic upregulation of smooth muscle specific actin and extracellular matrix proteins.The result is a fibrotic,stiff,and dysfunctional liver.Therefore,understanding the molecular mechanisms that govern HSC function is essential for the development of anti-fibrotic medications.The actin cytoskeleton has emerged as a key component of the fibrogenic response in wound healing.Recent data indicate that the cytoskeleton receives signals from the cellular microenvironment and translates them to cellular function—in particular,increased type I collagen expression.Dynamic in nature,the actin cytoskeleton continuously polymerizes and depolymerizes in response to changes in the cellular microenvironment.In this viewpoint,we discuss the recent developments underlying cytoskeletal actin dynamics in liver fibrosis,including how the cellular microenvironment affects HSC function and the molecular mechanisms that regulate the actininduced increase in collagen expression typical of activated HSCs. | NOUR HIJAZI DON C.ROCKEY ZENGDUN SHI | 2022 | BIOCELL2022，46，9： | 2 |
| 19 | MicroRNA expression profile and lipid metabolism characteristics in liver of rat undergoing high-fat diet显示文摘This study aimed to investigate the microRNA expression profile and the characteristics of lipid metabolism in the livers of rats undergoing a high-fat diet.Fifty male Sprague-Dawley(SD)rats were divided into a standard chow group(C group,N=10)and a high-fat diet group(H group,N=40).After 12 weeks,the rat body weight,body length,fat mass,and serum lipid concentration were measured.The expression profile of microRNAs and the gene and protein expression levels involved in lipid metabolism in rat liver were detected.Body fat and serum lipid concentrations were all significantly higher in the H group than those in the C group(p<0.05 or p<0.01).The expression of 10 microRNAs showed significant differences in the liver(p<0.05).In particular,the let-7 family expression levels significantly increased(p<0.05)in the H group compared with those in the C group.Compared with the C group,the high-fat diet resulted in low FAS,CPT1A,and ApoAI mRNA expression levels(p<0.05 or p<0.01)and high PPARαand FAT/CD36 mRNA expression levels in the H group rat liver(p<0.01).Meanwhile,the protein PPARα,FAS,CPT1A,FAT/CD36,and ApoAI expression levels were all significantly lower in the H group than those in the C group(p<0.05 or p<0.01).In conclusion,the high-fat diet increased the body fat and serum lipid levels and altered the 10 microRNA expression levels in the liver.The high-fat diet may affect hepatic carbohydrate metabolism and increase ectopic fat accumulation through let-7 family overexpression.The high-fat diet for 12 weeks decreased lipid metabolism level in the liver,thereby decreasing fatty acid synthesis,oxidation,and transport by down-regulating the PPARα,FAS,CPT1A,FAT/CD36,and ApoAI protein levels. | WEN JING CHUANFEN LI YINGLI LU LIANSHI FENG | 2019 | BIOCELL2019，43，3： | 2 |
| 20 | Exosomes derived from osteoclasts under compression stress inhibit osteoblast differentiation显示文摘Orthodontic tooth movement is triggered by orthodontic force loading on the periodontal ligament and is achieved by alveolar bone remodeling,which is regulated by intimate crosstalk between osteoclastogenesis and osteoblast differentiation.Whether the communication between osteoclasts and osteoblasts is influenced by orthodontic compression stress requires further clarification.In this study,osteoclasts were differentiated for 10 days.On day 4 of differentiation,the number of pre-osteoclasts peaked,as determined by the increased expression of RANK and the number of multinucleated cells.After 24 h of compression stress loading,on day 4,the number of osteoclasts increased,and the optimal magnitude of stress to promote osteoclastogenesis was determined as 1 g/cm2.Moreover,the results of RNA-sequencing analysis showed that the miRNA expression profile changed markedly after compression loading and that many of the altered miRNAs were associated with cell communication functions.A series of indirect co-culture experiments showed an inhibitory effect of osteoclasts on osteoblast differentiation,especially after compression.Next,we added osteoclast-derived exosomes to hPDLSCs during osteoblast differentiation.Exosomes derived from osteoclasts under compression(cEXO)showed a greater inhibitory effect on osteoblast differentiation,compared to exosomes from osteoclasts without compression(EXO).Therefore,we analyzed differentially expressed miRNAs associated with bone development functions in exosomes:miR-223-5p and miR-181a-5p were downregulated,whereas miR-133a-3p,miR-203a-3p,miR-106a-5p,and miR-331-3p were upregulated;these altered expressions may explain the enhanced inhibitory effect of compression stress. | YUE WANG YUNFEI ZHENG WEIRAN LI | 2021 | BIOCELL2021，45，2： | 2 |
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