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| 1 | αV integrin:A new gastrin target in human pancreatic cancer cells显示文摘AIM:To analyse αV integrin expression induced by gas-trin in pancreatic cancer models. METHODS:αV integrin mRNA expression in human pan-creatic cancer cells was analysed using a 'cancer genes' array and confirmed by real-time reverse transcription-polymerase chain reaction (PCR). Western blotting and semi-quantitative immunohistochemistry were used to examine protein levels in human pancreatic cancer cell lines and pancreatic tissues, respectively. The role of αV integrin on gastrin-induced cell adhesion was examined using blocking anti-αV integrin monoclonal antibodies. Adherent cells were quantified by staining with crystal violet.RESULTS: Using a 'cancer genes' array we identified αV integrin as a new gastrin target gene in human pancreatic cancer cells. A quantitative real-time PCR approach was used to confirm αV integrin gene expression. We also demonstrate that Src family kinases and the PI 3-kinase, two signalling pathways specifically activated by the CCK-2 receptor (CCK2R), are involved in gastrin-mediated αV integrin expression. In contrast, inhibition of the ERK pathway was without any effect on αV integrin expression induced by gastrin. Our results also show that gastrin modulates cell adhesion via α V integrins. Indeed, in vitro adhesion assays performed on fibronectin show that gastrin significantly increases adhesion of pancreatic cancer cells. The use of blocking anti-αV integrin monoclonal antibodies completely reversed the increase in cell-substrate adhesion induced by gastrin. In addition, we showed in vivo that the targeted CCK2R expression in the pancreas of Elas-CCK2 mice, leads to the overexpression of αV integrin. This process may contribute to pancreatic tumour development observed in these transgenic animals. CONCLUSION:αV integrin is a new gastrin target in pancreatic cancer models and contributes to gastrin effects on cell adhesion. | Celine Cayrol Claudine Bertrand Aline Kowalski-Chauvel Laurence Daulhac Elizabeth Cohen-Jonathan-Moyal Audrey Ferrand Catherine Seva | 2011 | World Journal of Gastroenterology2011，17，40： | 4 |
| 2 | Prevalence and risk factors of infections by multiresistant bacteria in cirrhosis: A prospective study显示文摘 | Javier Fernández Juan Acevedo Miriam Castro Orlando Garcia Carlos Rodríguez de Lope Daria Roca Marco Pavesi Elsa Sola Leticia Moreira Anibal Silva Tiago Seva‐Pereira Francesco Corradi Jose Mensa Pere Ginès Vicente Arroyo | 2012 | Hepatology2012，，： | 4 |
| 3 | Mechanism for Src activation by the CCK2 receptor:Patho-physiological functions of this receptor in pancreas显示文摘瞄准:调查在活体内, CCK2 受体(CCK2R ) 是否调整蛋白质,知道在房间增长和癌症开发和分析在试管内起一个关键作用导致 Src 激活的分子的机制;特别地,那在这激活被含有在 CCK2R 顺序以内识别领域。方法:Src 和英皇家空军之阶级最低之兵的表示和激活是学习在活体内使用免疫荧光和西方污点的技术。我们使用了从在胰腺的腺泡表示了 CCK2R 的野类型或 Elas-CCK2 老鼠导出的胰腺的纸巾,显示了增加的胰腺的生长并且开发了肿瘤前损害。短暂地表示内长的 CCK2R 或 COS-7 房间的胰腺的肿瘤房间线 AR4-2J 有野类型或变异的 CCK2R 的 transfected 被用作在试管内模型学习 Src 激活的机制。Src 激活被在试管内激酶试金测量,由用 anti-phospho-ERK 抗体和在由 MTT 的导致 gastrin 的房间增长的 Src 的参与的西方的污点的英皇家空军之阶级最低之兵激活测试。结果:我们出现了因为在 Elas-CCK2 鼠标的胰的指向的 CCK2R 表达式,导致了 Src 和 ERK 小径的激活。Src 被激活由在胰腺的弦音器的 CCK2R 的 ERK 小径在上游口头的房间并且贡献了增生的效果由这受体调停了。在试管内结果证明由 CCK2R 的 Src/ERK 小径的激活要求了 NPXXY 主题,在第 7 transmembrane 领域的结束在 CCK2R 顺序以内定位了,并且建议了在这机制的 Gq 的通常认为的角色。结论:由 CCK2R 的 Src/ERK 小径的解除管制可能代表贡献房间增长,肿瘤前损害的形成和胰腺的肿瘤开发的早步。 | Audrey Ferrand Sebastien Vatinel Aline Kowalski-Chauvel Claudine Bertrand Chantal Escrieut Daniel Fourmy Marlene Dufresne Catherine Seva | 2006 | World Journal of Gastroenterology2006，12，28： | 2 |
| 4 | Glycine-extended gastrin activates two independent tyrosine-kinases in upstream of p85/p110 phosphatidylinositol 3-kinase in human colonic tumour cells显示文摘瞄准:为了调查 Src, JAK2 和 phosphatidylinositol 3-kinase (PI3K ) 小径是否涉及人的结肠的瘤房间的增长,由扩大 glycine 的促胃液素导致了(G-gly ) ,先锋在 ated 促胃液素中并且到成熟阐明在响应这肽的这三家族 ases 之间的分子的相互作用。方法:用人的结肠的瘤,房间作为一个模型衬里 HCT116,我们首先测量了 PI3K, p60-Src 和 JAK2 的激活响应 G-gly 由在试管内激酶试金。然后,我们由 MTT 测试在 G-gly-induced 房间增长调查了这些家族 ases 的参与。结果:G-gly,刺激在 HCT116 导致了 p60-Src, JAK2 和 PI3K 激活。不同小径涉及房间导致了由的人的结肠癌的增长 G-gly。而且,我们发现 Src 和 JAK2 对由这肽的 PI3K 规定必要。然而,我们没发现在二酷氨酸家族 ases 之间的任何串音。结论:我们的结果建议 p60-Src/PI3K 和 JAK2/PI3K 小径独立地行动调停人的结肠的瘤房间上的 G-gly 增生的的效果。 | Audrey Ferrand Aline Kowalski-Chauvel Claudine Bertrand Daniel Fourmy Marlene Dufresne Catherine Seva Julie Pannequin | 2006 | World Journal of Gastroenterology2006，12，12： | 2 |
| 5 | Dynamic rotational seeding and cell culture systure for vascularturb formation显示文摘 | Nasseri BA Pomerant seva I Kaazem-Mof rad MR | | 0，，： | 1 |
| 6 | FIRE : an SPSS program for variable selection in multiple linear regression analysis via the relative importance of predictors显示文摘 | LORENZO Seva U FERRANDO P J | 2010 | Behavior Research Methods2010，43，1： | 1 |
| 7 | Cholecystokinin and gastrin receptors 显示文摘 | DUFRESNE M SEVA C FOURMY D | 2006 | Physiol Rev2006，86，3： | 1 |
| 8 | Effect of eleutherococcus on subcelluar heart structures in experimental mycocardial infraction显示文摘 | Afanas seva TN Lebkove NP | 1987 | Byulleten Eksperinental Biologii Meditsiny1987，103，2： | 1 |
| 9 | Stimulation of rat pancreatic tumoral AR4-2J cell proliferation by pituitary adenylate cyclaseactivating peptide显示文摘 | Cambillau C Seva C | 1992 | Gastroenterology1992，103，3： | 1 |
| 10 | Is Sweden Being Tom Apart? Privatiza- tion and Old and New Patterns of Welfare Support显示文摘 | EDIUND J SEVA I J | 2013 | Social Policy and Administration2013，47，5： | 1 |
| 11 | Gastrin and glycine-extended progastrin processing intermedicates induce different programs of early gene activation 显示文摘 | Todisco A Takeuchi Y Seva C | 1995 | J Bilog Chem1995，270，28： | 1 |
| 12 | Five days of ceftriaxone to treat spontaneous bacterial peritonitis in cirrhotic patients显示文摘 | Franca A Giordano HM Seva Pereira T | 2002 | J-Gastroenterol2002，37，2： | 1 |
| 13 | Somatostatin inhibits AP-1 function via multiple protein phosphatases显示文摘 | Todisco A Seva C Takeuchi Y | 1995 | Am J Physiol1995，269，10： | 1 |
| 14 | Peptides trap the human immunodeficiency virus type 1 envelop glycoprotein fusion intermediate at two sites显示文摘 | He Y Vassell R Zait seva M | 2003 | J Virol2003，77，： | 1 |
| 15 | Zinc and cop- per content in developing and aging coleoptiles of wheat seedlings显示文摘 | Smirnova T A Kolomiit Seva G Y Prusov A N | 2006 | Russian Journal of Plant Physiology2006，53，4： | 1 |
| 16 | Product design enhancement using apparent usability and affective quality 显示文摘 | SEVA R R GOSIACO K G SANTOS M C | 2011 | Applied ergonomics2011，，3： | 1 |
| 17 | Growth-promoting effects of glycine-extended progastrin 显示文摘 | Seva C Dickinson CJ Yamada T | 1994 | Science1994，265，： | 1 |
| 18 | Product design enhancement using apparent usability and affective quality显示文摘 | SEVA R R GOSIACO K G T SANTOS M C E D | 2011 | Applied Ergonomics2011，42，3： | 1 |
| 19 | Growth promoting effects of glycine-extended progastrin显示文摘 | Seva C Dickinson CT Yamada T | 1994 | Science1994，265，： | 1 |
| 20 | Gastrin and glycine-extended progastrin processing intermediates induce different programs of early gene activation显示文摘 | Todisco A Takeuchi Y Seva C | 1995 | J Biol Chem1995，270，28： | 1 |
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