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| 1 | Effectiveness of external Sanjierupi Gao on mastalgia caused by mammary gland hyperplasia: a placebo controlled trial显示文摘OBJECTIVE:To evaluate the curative effect of external application of the Chinese drug,Sanjierupi Gao,on mastalgia caused by mammary gland hyperplasia.METHODS:This randomized,double-blinded,and placebo controlled study enrolled 260 patients with mammary gland hyperplasia from five hospitals.Patients were randomly and equally divided in-to a Sanjierupi Gao treatment group and a placebo control group.An adhesive plaster was applied to the most painful area on either breast for 7 h a day.Treatment lasted for two menstrual cycles without application during menstruation.Mastalgia was used as the main index of curative effect.The change before and after treatment in days of mastalgia,the time to alleviate pain,pain extent,and severe pain scores were observed.RESULTS:Compared to the control group,the treatment group had significantly fewer days of mastalgia(P<0.01),a significantly lower severe pain score(P<0.01),and significantly less subjective pain and tenderness(P<0.05 and P<0.01,respectively).Three days before the follow-up visit,the pain score in the treatment group was significantly lower than that in the control group(P<0.05).A non-parametric test was used to compare the time to alleviate mastalgia between the two groups and found no statistical difference(Z=0.313,P=0.754).CONCLUSION:Application of Sanjierupi Gao can decrease mastalgia duration in patients with mammary gland hyperplasia during menstruation and alleviate the extent of mastalgia.The time to alleviate pain is psychologically influenced. | Yingyi Fan Xiaohua Pei Zhaolan Liu Zhongyuan Xia Dongxiao Zhang Afeng Song Duo Liu | 2013 | Journal of Traditional Chinese Medicine2013，33，5： | 7 |
| 2 | Preparation and modulation of a novel thin-walled carbon foam显示文摘By foaming and carbonization processes under atmospheric pressure, a novel thin-walled carbon foam with developed foam structure was successfully prepared from loose medium component(LMC) separated from raw coal by extraction and back-extraction method. The influences of foaming time, carbonization time, and micromolecule content on foam structure were investigated by scanning electron microscope and mercury injection data. Moreover, foaming mechanism of LMC was analyzed and expounded. The results showed that spherical pores and uniform ultrathin pore walls constitute threedimensional foam structure of carbon foam and foam structure is developed with well connectivity.The effects of foaming time, carbonization time, and micromolecule content on foam structure are significant. Especially, average pore diameters of carbon foams prepared from the extracts of LMC are much smaller. With the rise of extraction rate, average pore diameter decreases and pore size distribution is more concentrated on the aperture section of 0–10 μm. | Zhihong Qin Peng Chang Lingling Ma Lianghui Bu Zhaolan Song | 2019 | International Journal of Mining Science and Technology2019，29，2： | 3 |
| 3 | Characterization of Pythium chondricola associated with red rot disease of Pyropia yezoensis (Ueda)(Bangiales,Rhodophyta) from Lianyungang,China显示文摘Pyropia yezoensis(formerly Porphyra yezoensis)is an economically important red alga that is cultured extensively in China.The red rot disease occurs commonly during Pyropia cultivation,causing serious economic losses.An incidence of red rot disease was found in a P.yezoensis farm from mid-November to mid-December 2015 at Lianyungang,Jiangsu Province,China.Histopathological examination revealed that the naturally infected thalli were infected apparently by a pathogen,leading to red rot symptoms.The causative agent was isolated and identified as the oomycete Pythium chondricola by morphological analysis and sequence analysis of the internal transcribed spacer and cytochrome oxidase subunit 1(cox 1).In artifi cial infection experiments on the P.yezoensis blades,the P.chondricola isolate was able to cause the same characteristic histopathology seen in natural infections.P.chondricola grew well at a wide range of temperatures in the range 8-31℃,salinities at 0-45 and pH 5-9.In an orthogonal test used to determine the effects of environmental factors(temperature,salinity,and zoospore concentration)on infection,the data revealed that temperature was the most important factor to affect red rot disease development,with the optimal conditions for disease expansion being 20℃,35 salinity,and a zoospore concentration of 10^6 zoospores/mL.The results obtained from the present study prompted us to set up a comprehensive epidemiological study on Pyropia,which will provide support to maintain the healthy development of the Pyropia industry in China. | QIU Liping MAO Yunxiang TANG Lei TANG Xianghai MO Zhaolan | 2019 | Journal of Oceanology and Limnology2019，37，3： | 3 |
| 4 | Rapid detection of virulent protease secreted by Vibrio anguillarum by dot enzyme-linked immunosorbent assay显示文摘Dot enzyme-linked immunosorbent assay (dot-ELISA), indirect ELISA and Western blot were performed to detect the virulent protease secreted by Vibrio anguillarum which was isolated from the diseased left-eyed flounder, Paralichthys olivaceous. Sensitivity results showed that dot-ELISA is a more sensitive, rapid and simple technique for the protease detection. The minimal detectable amount of protease is about 7 pg in the dot-ELISA test, while 7.8 ng in the indirect ELISA and 6.25 ng in the Western blot respectively. Protease could be detected 2 h after incubation of V. anguillarum in the 2216E liquid medium but enzyme activity was very low at that period.From 6 to 12 h, the amount and enzyme activity ofprotease increased markedly and reached maximum at stationary phase. Analysis of serum samples periodically collected from the infected flounders showed that after 2 h of infection by V. anguillarum, the pathogenic bacteria could be detected in the blood of the infected flounders but no protease was found. It was 5~6 h after infection that the protease was detected in blood and then the amount increased as infection advanced. Quantitative detection of protease either incubation in the medium or from the blood of infected flounders could be accomplished in virtue of positive controls of quantificational protease standards ('marker') so that the alterations of protease secretion both in vitro and in vivo could be understood generally. In addition, the indirect ELISA and dot-ELISA were also performed to detect V. anguillarum cells. Results indicated that the sensitivity of indirect ELISA to bacteria cells is higher than that of the dot-ELISA, and that the minimal detectable amount is approximately 104 cell/mL in the indirect ELISA, while 105 cell/mL in the dot-ELISA. | ZHANG Zhendong ZHANG Peijun MO Zhaolan WANG Chunling YU Yang | 2005 | Acta Oceanologica Sinica2005，24，4： | 2 |
| 5 | Chinese herbal medicines for treatment of hand, foot and mouth disease: A systematic review of randomized clinical trials显示文摘 | Huijuan Cao Zhaolan Liu Peter Steinmann Yujie Mu Hui Luo Jianping Liu | 2011 | European Journal of Integrative Medicine2011，，1： | 2 |
| 6 | The neural circuit basis of Rett syndrome显示文摘 | Darren GOFFIN Zhaolan (Joe) ZHOU | 2012 | Frontiers of physics2012，7，5： | 1 |
| 7 | Chemical structure of monascus polysaccharide 显示文摘 | Tian Jun Li Zhaolan Shen Xiaoyun | 1998 | Journal of Nanjing University of Traditional Chinese Medicine:Natural Science Edition1998，14，4： | 1 |
| 8 | Preparation and Identification of Monoclonal Antibodies Against Vibrio anguillarum显示文摘Monoclonal antibodies (Mabs) against V.anguillarum strain M3 are prepared, and their isotypes are also characterized. Among them, C1C5 is the only Mab which does not crossreact with other eleven non-V.anguillarum strains. The proteinase K digestion test shows that the epitopes recognized by C1C5, C6C3 and C6C32 Mabs contained protein. The periodate oxidation test showed that the epitopes recognized by Mabs except C1C5 are glycosylated. In addition, results of additivity test indicate that the epitopes recognized by C6C3 and C6C32 Mabs are similar, and quite different from those recognized by Mab C1C5. | 陈师勇 Zhang Peijun Mo Zhaolan Zhang Zhendong Zou Yuxia Xu Yongli | 2004 | High Technology Letters2004，10，1： | 1 |
| 9 | Obtaining of cowpea trypsin inhibitor transgenic tabacco with insect resistance显示文摘 | Liu Chunming Zhu zhen Zhou Zhaolan | 1992 | Chin Sci Bull1992，，18： | 1 |
| 10 | Properties of Klebsiella Phage P13 and Associated Exopolysaccharide Depolymerase显示文摘The bacteriophage P13 that infects Klebsiella serotype K13 contains a heat-stable depolymerase capable of effective degradation of exopolysaccharide(EPS) produced by this microorganism. In this study, the titer of phage P13, initially 2.0 × 107 pfu mL-1, was found increasing 20 min after infection and reached 5.0 × 109 pfu mL-1 in 60 min. Accordingly, the enzyme activity of depolymerase approached the maximum 60 min after infection. Treatment at 70℃ for 30 min inactivated all the phage, but retained over 90% of the depolymerase activity. Addition of acetone into the crude phage lysate led to precipitation of the protein, with a marked increase in bacterial EPS degradation activity and a rapid drop in the titer of phage. After partial purification by acetone precipitation and ultrafiltration centrifugation, the enzyme was separated from the phage particles, showing two components with enzyme activity on Q-Sepharose Fast Flow. The soluble enzyme had an optimum degradation activity at 60℃ and pH 6.5. Transmission electron microscopy demonstrated that the phage P13 particles were spherical with a diameter of 50 nm and a short stumpy tail. It was a double-strand DNA virus consisting of a nucleic acid molecule of 45976 bp. This work provides an efficient purification operation including thermal treatment and ultrafiltration centrifugation, to dissociate depolymerase from phage particles. The characterization of phage P13 and associated EPS depolymerase is beneficial for further application of this enzyme. | LIU Yang LI Guiyang MO Zhaolan CHAI Zihan SHANG Anqi MOU Haijin | 2014 | Journal of Ocean University of China2014，13，1： | 1 |
| 11 | Evidence-based toxicity evaluation and scheduling of Chinese herbal medicines显示文摘 | Ellie J.Y. Kim Yuling Chen Johnson Q. Huang Kong M. Li Valentina Razmovski-Naumovski Josiah Poon Kelvin Chan Basil D. Roufogalis Andrew J. McLachlan Sui-Lin Mo Depo Yang Meicun Yao Zhaolan Liu Jianping Liu George Q. Li | 2013 | Journal of Ethnopharmacology2013，，1： | 1 |
| 12 | Characterization of EmpA protease in Vibrio anguillarum M3显示文摘EmpA is an extracellular metalloprotease secreted by Vibrio anguillarum.For better understanding its role in the patho-genicity of V.anguillarum strain M3,empA insertion mutant was constructed.In the mutant it decreased in extracellular proteolytic activity,swarming motility,hemolytic activity and virulence on turbot(Scophthalmus maximus).Significant decline(by 5-fold)of extracellular proteolytic activity and similar growth curve between mutant and wild type strains indicated that EmpA was the major extracellular protease of M3.LD50 of mutant increased by 38-fold compared with wild type.No pro-EmpA was detected in the su-pernatant of culture,indicating that EmpA autolyzed to mature protein after 24 h.Secretion of EmpA in M3 was similar to that in NB10 strain.Attenuated virulence of mutant was similar to that of M93Sm strain.It was demonstrated that specific operation of EmpA was different from that in previous studies and EmpA contributed to the swarming motility and hemolytic activity in V.an-guillarum strain M3.The results provides insight into understanding the function of EmpA and its potential application in vaccine development. | HAN Yifan MO Zhaolan XIAO Peng HAO Bin LI Jie YANG Guanpin | 2011 | Journal of Ocean University of China2011，10，4： | 1 |
| 13 | Super-Edge-Connectivity of G(k,d,s)(s≥k/2)显示文摘A graph G is super-edge-connected,for short super-λ,if every minimum edge-cut consists of edges adjacent to a vertex of minimum degree.Alphabet overlap graph G(k,d,s)is undirected,simple graph with vertex set V={v|v=1()kv…v;vi∈{1,2,…,d},i=1,…,k}.Two vertices u=(u1…uk)and v=(v1…vk)are adjacent if and only if us+i=vi or vs+i=ui(i=1,…,k-s).In particular G(k,d,1)is just an undirected de Bruijn graph.In this paper,we show that the diameter of G(k,d,s)is k s,the girth is 3.Finally,we prove that G(k,d,s)(s≥k/2)is super-λ. | YANG Zhaolan HUANG Shihua YANG Rong | 2014 | Wuhan University Journal of Natural Sciences2014，19，3： | 0 |
| 14 | Detection of Vibrio anguillarum and Its Virulent Metalloprotease Using the Method of ELISA显示文摘A method detecting pathogenic Vibrio anguillarum and its virulent metalloprotease is reported in this paper. The metalloprotease is isolated from extracellular product (ECP) of V.anguillarum by the cellophane plate technique and purified by gel filtration and ion exchange chromatography. Anti sera are prepared by injecting V.anguillarum cells and metalloprotease into the rabbits. Slide Agglutination Assay is used to detect V.anguillarum in the infection experiment and Enzyme Linked Immunosorbent Assay (ELISA) is carried out to detect concentration of metalloprotease. The result shows that bacterium strain M3 is able to diffuse into the viscera of infected fish through the blood circulating system 10 hours after intramuscular infection, and ELASA is a sensitive method to detect the metalloprotease with detectable amount of 7.8ng. The aim of this study is to establish a sensitive and specific method to observe the infection of V.anguillarum in the host. | 张振冬 Zhang Peijun Mo Zhaolan Chen Shiyong | 2002 | High Technology Letters2002，8，4： | 0 |
| 15 | Discrete sliding mode prediction control of uncertain switched systems显示文摘The robust stabilization problem for a class of uncertain discrete-time switched systems is presented. A predictive sliding mode control strategy is proposed,and a discrete-time reaching law is improved.By applying a predictive sliding surface and a reference trajectory,combining with the state feedback correction and rolling optimization method in the predictive control strategy,a predictive sliding mode controller is synthesized,which guarantees the asymptotic stability for the closed-loop systems.The designed control strategy has stronger robustness and chattering reduction property to conquer with the system uncertainties.In addition,a unique nonswitched sliding surface is designed.The reason is to avoid the repetitive jump of the trajectories of the state components of the closed-loop system between sliding surfaces because it might cause the possible instability.Finally,a numerical example is given to illustrate the effectiveness of the proposed theory. | He Zhaolan Wang Mao Liu Shuhuan | 2009 | Journal of Systems Engineering and Electronics2009，20，5： | 0 |
| 16 | Regulatory Effects of Xylooligosaccharides on Intestinal Microbial Flora Proliferation and Defecation of BALB/c Mice显示文摘[Objective] This study aimed to investigate the regulatory effects of xylooligosaccharides on intestinal microbial flora proliferation and defecation of BALB/c mice.[Method] In the intestinal microbial flora proliferation regulation experiment, microflora quantities of Bifidobacterium, Lactobacillus, Enterobacter and Enterococci in excrements of BALB/c mice were determined after continuous administration of high-dose[1.0 g/(kg·bw)] and low-dose[0.5 g/(kg·bw)] xylooligosaccharides for 14 d.In the defecation regulation experiment, constipation model of BALB/c mice was established with compound diphenoxylate after continuous administration of high-dose and low-dose xylooligosaccharides for 21 d, to determine the first defecation time of black excrement, weight of black excrements within 6 h and rate of intestinal propulsion of constipation mice.[Result] Compared with the control group, after continuous administration of xylooligosaccharides for 14 d, microflora quantities of Lactobacillus and Bifidobacterium in excrements of BALB/c mice in low-dose and high-dose groups both increased significantly (P<0.05).After continuous administration of xylooligosaccharides, microflora quantities of Enterococci and Lactobacillus in control group were significantly raised (P<0.05 and P<0.01, respectively) compared with those before administration; microflora quantities of Enterococci in high-dose and low-dose groups were significantly reduced (P<0.05), while microflora quantities of Lactobacillus and Bifidobacterium and the B/E value increased significantly (P<0.05, P<0.01 and P<0.01, respectively).After continuous administration of xylooligosaccharides for 21 d, compared with the model group, the first defecation time of black excrement in high-dose and low-dose groups was significantly shortened (P<0.05), the total weight of black excrements within 6 h increased significantly (P<0.05) and the rate of intestinal propulsion was significantly enhanced (P<0.01).[Conclusion] Low-dose (0.5 g/Kg.bw) and high-dose (1 g/Kg.bw) xylooligosaccharides can improve the proliferation of intestinal microbial flora and prevent mice from constipation. | Tao WEI Hongtao SHANG Zhaolan GAO Li WANG Siyu LI Xue HUANG Mengrui ZHENG | 2013 | Agricultural Biotechnology2013，2，3： | 0 |
| 17 | Temporal Behaviours of Stimulated Rayleigh-Wing Scattering at Non- and Quasi-Equilibrium of Orientational Distribution显示文摘The temporal behaviours of stimulated Rayleigh-wing scattering,at quasi-equilibrium and non-equilibrium of the molecular orientational distributions,are reported in this letter.The experimental results also show the relaxation time of molecular orientation in high alignment is longer than 80 picosecond. | WANG Hezhou ZHENG Xiguang MAO Weidong YU Zhenxin GAO Zhaolan | 1995 | Chinese Physics Letters1995，12，9： | 0 |
| 18 | Molecular and genetic insights into an infantile epileptic encephalopathy - CDKL5 disorder显示文摘背景发现在 cyclin 依赖的象 kinase 一样的变化 5 (CDKL5 ) 基因与婴儿的癫痫的 encephalopathy 被联系刺激了世界范围的研究努力理解 CDKL5 的分子、基因的基础混乱。给远这样出版的文学的大数字,这评论试图总结当前的基因研究,在建议分子的功能上拉一致,并且指向在 CDKL5 研究的知识的差距。一个系统的评论过程用 PubMed 搜索引擎被进行集中于 CDKL5 研究在的方法最近十年。我们分析了这些出版物并且总结了调查结果进四节:基因研究, CDKL5 表达式模式,分子的函数,和动物模型。我们也在每节讨论了挑战和未来方向。临床的方面上的结果, CDKL5 混乱被早发作描绘癫痫的发作,智力的残疾,和 stereotypical 行为。在研究方面,在人的病人的一系列分子、基因的研究,房间文化和动物上,模型建立了 CDKL5 的诱发性到婴儿的癫痫的 encephalopathy,并且在在大脑调整 neuronal 功能为 CDKL5 指向了一个关键角色。CDKL5 混乱的老鼠模型也被开发了,并且尤其是,表明行为的显型, CDKL5 混乱的众多的临床的症状和进展 CDKL5 研究到现出症状之前的潜的阶段的 mimicking。我们远这样学习了的给的结论,柔韧、量、敏感的结果措施的未来鉴定将是在动物模型研究的钥匙,特别地在异质接合的女性。同时, CDKL5 的分子、细胞的研究应该集中于基于机制的调查和目的揭开提供潜力救或改善的 druggable 目标 CDKL5 混乱相关的显型。 | Ailing Zhou Song Han Zhaolan Joe Zhou | 2017 | Frontiers in Biology2017，12，1： | 0 |
| 19 | Development of a PCR method for detection of Pseudoalteromonas marina associated with green spot disease in Pyropia yezoensis显示文摘Pseudoalteromonas marina is one of the potential pathogens that cause green spot disease(GSD)in Pyropia yezoensis.To prevent GSD from development and spread,an effective method to detect the pathogen at early GSD infection stages need to be established.In this research,PCR methods were established targeting the dnaA gene(encoding chromosome replication initiator protein)and the dnaN gene(encodingβsliding clamp of DNA polymeraseⅢprotein)to detect P.marina with three primer pairs pws-dnaA2(Forward,5'-ACCGCATTAACGAACTACTCGTG-3';Reverse,5'-TGCCATTACCTACAGCATGG-3'),pcs-dnaN2(Forward,5'-CTTACAACGTTATCAGCGGC-3';Reverse,5'-GTTGAGTATTAAGTGATTGAGTAAGC-3')or pws-dnaN3(Forward,5'-ACTTACAA-CGTTATCAGCGGC-3';Reverse,5'-ACTGCTGTTTGAGTCTGCTAAC-3').Three PCR methods corresponding to the three primer pairs sufficiently distinguished P.marina from 22 bacterial species,thus resulting in detection limits of 4 to 4×10^2 CFU cells or 2.37×10^1 to 2.37×10^3 fg of P.marina DNA per PCR reaction.In an artificial infection experiment ofP.yezoensis infected with P.marina,all established PCRs successfully detected P.marina at early GSD infection stages.The results show that the established PCRs are specific and sensitive,and are potential for applications in early diagnosis of GSD in Pyropia. | YANG Huichao YAN Yongwei LI Jie TANG Lei MAO Yunxiang MO Zhaolan | 2020 | Journal of Oceanology and Limnology2020，38，1： | 0 |
| 20 | Locus- and cell type-specific epigenetic switching during cellular differentiation in mammals显示文摘包括在 DNA methylation 和 histone 修正的变化,背景 Epigenomic 重构为胚胎的干细胞(转换字符) 的区别是关键的进体的房间。然而, epigenome 被重新设置到的程度和在 epigenome 的部件之间的相互影响在细胞的区别期间仍然保持糟糕定义。我们系统地从人和老鼠体的房间的二种不同类型与那些在转换字符分析了并且比较 DNA methylation,各种各样的 histone 修正,和 transcriptome 侧面的方法。我们发现了那全球 DNA methylation 的结果层次在与在种类的转换字符相比的体的房间是更低的。我们也发现有 histone 修正占有的 80% 区域在人的转换字符和二种人的体的房间类型之间不同。在 DNA methylation 和 histone 修正的约 70% 重构是地点 -- 并且房间 typespecific。有趣地, DNA methylation 的损失被不同 histone 修正的获得在一个地点伴随 -- 并且房间类型特定的举止。在倡导者区域与 epigenetic 重构联系的 transcriptional 变化的进一步的检查揭示了切换的 epigenetic 因为从稳定的基因 silencing 的基因 regulationa 转变在转换字符由 DNA methylation 调停了到压抑的 histone 修正在体的房间便于的灵活基因压抑。结论我们的调查结果证明 epigenome 在一个地点被重新设置 -- 并且类型特定的举止并且 epigenetic 切换是的房间在在人和老鼠的细胞的区别期间普通。 | Ying-Tao Zhao Maria Fasolino Zhaolan Zhou | 2016 | Frontiers in Biology2016，11，4： | 0 |
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