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| 1 | Isolation of Tyl-copia-like Retrotransposon Sequences from the Apple Genome by Chromosome Walking Based on Modified SiteFinding-polymerase Chain Reaction显示文摘长终端重复(LTR ) retrotransposons 是为学习基因生物多样性,染色体进化,基因变化,克隆的基因和基因表达的强大的工具。缺乏 ofretrotransposon 顺序信息限制在在场的更高的 plants.Inthe 的研究学习的这些的发展, Ty1-copia-like retrotransposons 的 31 逆[转]录酶( RT )基因被放大编码区域使用的 RT 从苹果染色体识别 degenerateprimers.Nineteen RT 基因以碎片尺寸显示出极端异质,基础对作文和开的读框架完全。从一 266 bp 发源克隆 RT 基因, 1966 bpTy1-copia-like retrotransposon (命名 Tcrm1 )包括 RT-ribonuclease H-LTR 领域序列,被染色体在基因结构和 Tcrml 是包括在揭示的 applegenome.Dot 污点分析的一个 LTR 领域的第一 Ty1-copia-like retrotransposon 的顺序相同 hows 基于在 Tcrml 和另外的 LTR retrotransposons 之间的修改SiteFinding聚合酶链 reaction.Thecomparison 走完成在苹果的那个 Tcrm1 拷贝数字每 haploid 是近似 1x10 ~ 3copies 染色体。 | Guiling ZHAO Zhihong ZHANG Haiyue SUN He LI Hongyan DAI | 2007 | Acta Biochimica et Biophysica Sinica2007，39，9： | 8 |
| 2 | Genomic insights into the fast growth of paulownias and the formation of Paulownia witches' broom显示文摘Paulownias are among the fastest growing trees in the world,but they often suffer tremendous loss of wood production due to infection by Paulownia witches'broom(PaWB)phytoplasmas.In this study,we have sequenced and assembled a high-quality nuclear genome of Paulownia fortunei,a commonly cultivated paulownia species.The assembled genome of P.fortunei is 511.6 Mb in size,with 93.2%of its sequences anchored to 20 pseudo-chromosomes,and it contains 31985 protein-coding genes.Phylogenomic analyses show that the family Paulowniaceae is sister to a clade composed of Phrymaceae and Orobanchaceae.Higher photosynthetic efficiency is achieved by integrating C3 photosynthesis and the crassulacean acid metabolism pathway,which may contribute to the extremely fast growth habit of paulownia trees.Comparative transcriptome analyses reveal modules related to cambial growth and development,photosynthesis,and defense responses.Additional genome sequencing of PaWB phytoplasma,combined with functional analyses,indicates that the effector PaWB-SAP54 interacts directly with Paulownia PfSPLa,which in turn causes the degradation of PfSPLa by the ubiquitin-mediated pathway and leads to the formation of witches'broom.Taken together,these results provide significant insights into the biology of paulownias and the regulatory mechanism for the formation of PaWB. | Yabing Cao Guiling Sun Xiaoqiao Zhai Pingluo Xu Liming Ma Minjie Deng Zhenli Zhao Haibo Yang Yanpeng Dong Zhonghai Shang Yujie Lv Lijun Yan Haifang Liu Xibing Cao Bingbing Li Zhe Wang Xiaogai Zhao Haiyan Yu Fan Wang Wen Ma Jinling Huang Guoqiang Fan | 2021 | Molecular Plant2021，14，10： | 7 |
| 3 | Heat Aggregation Mechanisms of Hot Dry Rocks Resources in the Gonghe Basin, Northeastern Tibetan Plateau显示文摘Hot dry rock(HDR) is an important geothermal resource and clean energy source that may play an increasingly important role in future energy management. High-temperature HDR resources were recently detected in deep regions of the Gonghe Basin on the northeastern edge of the Tibetan Plateau, which led to a significant breakthrough in HDR resource exploration in China. This research analyzes the deep temperature distribution, radiogenic heat production, heat flow, and crustal thermal structure in the Qiaboqia Valley, Guide Plain, and Zhacanggou area of the Gonghe Basin based on geothermal exploration borehole logging data, rock thermophysical properties, and regional geophysical exploration data. The results are applied to discuss the heat accumulation mechanism of the HDR resources in the Gonghe Basin. The findings suggest that a low-velocity layer in the thickened crust of the Tibetan Plateau provides the most important source of constant intracrustal heat for the formation of HDR resources in the Gonghe Basin, whereas crustal thickening redistributes the concentrated layer of radioactive elements, which compensates for the relatively low heat production of the basal granite and serves as an important supplement to the heat of the HDR resources. The negative effect is that the downward curvature of the lithospheric upper mantle caused by crustal thickening leads to a small mantle heat flow component. As a result, the heat flows in the Qiaboqia Valley and Guide Plain of the Gonghe Basin are 106.2 and 77.6 m W/m2, respectively, in which the crust-mantle heat flow ratio of the former is 3.12:1, indicating a notably anomalous intracrustal thermal structure. In contrast, the crust-mantle heat flow ratio in the Guide Plain is 1.84:1, which reflects a typical hot crust-cold mantle thermal structure. The Guide Plain and Zhacanggou area show the same increasing temperature trend with depth, which reflects that their geothermal backgrounds and deep high-temperature environments are similar. These results provide important insight on the heat source mechanism of HDR resource formation in the Tibetan Plateau and useful guidance for future HDR resource exploration projects and target sites selection in similar areas. | LIN Wenjing WANG Guiling ZHANG Shengsheng ZHAO Zhen XING Linxiao GAN Haonan TAN Xianfeng | 2021 | Acta Geologica Sinica(English Edition)2021，95，6： | 5 |
| 4 | Genome-wide identification of agronomically important genes in outcrossing crops using OutcrossSeq显示文摘Many important crops(e.g.,tuber,root,and tree crops)are cross-pollinating.For these crops,no inbred lines are available for genetic study and breeding because they are self-incompatible,clonally propagated,or have a long generation time,making the identification of agronomically important genes difficult,particularly in crops with a complex autopolyploid genome.In this study,we developed a method,OutcrossSeq,for mapping agronomically important loci in outcrossing crops based on whole-genome low-coverage resequencing of a large genetic population,and designed three computation algorithms in OutcrossSeq for different types of outcrossing populations.We applied OutcrossSeq to a tuberous root crop(sweet potato,autopolyploid),a tree crop(walnut tree,highly heterozygous diploid),and hybrid crops(double-cross populations)to generate high-density genotype maps for the outcrossing populations,which enable precise identification of genomic loci underlying important agronomic traits.Candidate causative genes at these loci were detected based on functional clues.Taken together,our results indicate that OutcrossSeq is a robust and powerful method for identifying agronomically important genes in heterozygous species,including polyploids,in a cost-efficient way.The OutcrossSeq software and its instruction manual are available for downloading at www.xhhuanglab.cn/tool/OutcrossSeq.html. | Mengjiao Chen Weijuan Fan Feiyang Ji Hua Hua Jie Liu Mengxiao Yan Qingguo Ma Jiongjiong Fan Qin Wang Shufeng Zhang Guiling Liu Zhe Sun Changgeng Tian Fengling Zhao Jianli Zheng Qi Zhang Jiaxin Chen Jie Qiu Xin Wei Ziru Chen Peng Zhang Dong Pei Jun Yang Xuehui Huang | 2021 | Molecular Plant2021，14，4： | 4 |
| 5 | Nanocluster PtNiP supported on graphene as an efficient electrocatalyst for methanol oxidation reaction显示文摘In this study,phosphorus doped graphene supported PtNiP nanocluster electrocatalyst(PtNiP/P-graphene)was successfully prepared via a simple hypophosphite-assisted co-reduction method.The improved anchoring force and increased anchoring sites of graphene support result from phosphorus doping as well as size-confined growth effect of NaH_(2)PO_(2) leads to uniform dispersion of ultrafine PtNiP nanoclusters.Doped P also promotes the removal of CO-like intermediate by adjusting Pt electronic structure combining with alloyed Ni via electronic effects.As a result,the as-prepared PtNiP/P-graphene catalyst with more exposed active sites and optimized electronic structure of Pt alloy shows excellent electrocatalytic performances for methanol oxidation reaction(MOR)both in activity and durability in an acidic medium. | Long Yang Guoqiang Li Rongpeng Ma Shuai Hou Jinfa Chang Mingbo Ruan Wenbin Cai Zhao Jin Weilin Xu Guiling Wang Junjie Ge Changpeng Liu Wei Xing | 2021 | Nano Research2021，14，8： | 4 |
| 6 | Exercise promotes angiogenesis by enhancing endothelial cell fatty acid utilization via liver-derived extracellular vesicle miR-122-5p显示文摘Background:Angiogenesis constitutes a major mechanism responsible for exercise-induced beneficial effects.Our previous study identified a cluster of differentially expressed extracellular vesicle microRNAs(miRNAs)after exercise and found that some of them act as exerkines.However,whether these extracellular vesicle miRNAs mediate the exercise-induced angiogenesis remains unknown.Methods:A 9-day treadmill training was used as an exercise model in C57BL/6 mice.Liver-specific adeno-associated virus 8 was used to knock down microRNA-122-5p(miR-122-5p).Human umbilical vein endothelial cells were used in vitro.Results:Among these differentially expressed extracellular vesicle miRNAs,miR-122-5p was identified as a potent pro-angiogenic factor that activated vascular endothelial growth factor signaling and promoted angiogenesis both in vivo and in vitro.Exercise increased circulating levels of miR-122-5p,which was produced mainly by the liver and shuttled by extracellular vesicles in mice.Inhibition of circulating miR-122-5p or liver-specific knockdown of miR-122-5p significantly abolished the exercise-induced pro-angiogenic effect in skeletal muscles,and exerciseimproved muscle performance in mice.Mechanistically,miR-122-5p promoted angiogenesis through shifting substrate preference to fatty acids in endothelial cells,and miR-122-5p upregulated endothelial cell fatty-acid utilization by targeting 1-acyl-sn-glycerol-3-phosphate acyltransferase(AGPAT1).In addition,miR-122-5p increased capillary density in perilesional skin tissues and accelerated wound healing in mice.Conclusion:These findings demonstrated that exercise promotes angiogenesis through upregulation of liver-derived extracellular vesicle miR-122-5p,which enhances fatty acid utilization by targeting AGPAT1 in endothelial cells,highlighting the therapeutic potential of miR-122-5p in tissue repair. | Jing Lou Jie Wu Mengya Feng Xue Dang Guiling Wu Hongyan Yang Yan Wang Jia Li Yong Zhao Changhong Shi Jiankang Liu Lin Zhao Xing Zhang Feng Gao | 2022 | Journal of Sport and Health Science2022，11，4： | 4 |
| 7 | Multivalent Sn species synergistically favours the CO_(2)-into-HCOOH conversion显示文摘Although Sn-based catalysts have recently achieved considerable improvement in selective electro-catalyzing CO_(2)into HCOOH,the role of various valence Sn species is not fully understood due to the complexity and uncertainty of their evolution during the reaction process.Here,inspired by the theoretical simulations that the concomitant multivalent Sn(Sn^(0),Sn^(Ⅱ)and Sn^(Ⅳ))can significantly motivate the intrinsic activity of Sn-based catalyst,the Sn/SnO/SnO_(2)nanosheets were proposed to experimentally verify the synergistic effect of multivalent Sn species on the CO_(2)-into-HCOOH conversion.During CO_(2)reduction reaction,the Sn/SnO/SnO_(2)nanosheets,which are prepared by the sequential hydrothermal reaction,calcined crystallization and low-temperature H_(2)treatment,exhibit a high FEHCOOH of 89.6%at-0.9 VRHE as well as a large cathodic current density.Systematic experimental and theoretical results corroborate that multivalent Sn species synergistically energize the CO_(2)activation,the HCOO*adsorption,and the electron transfer,which make Sn/SnO/SnO_(2)favour the conversion from CO_(2)into HCOOH in both thermodynamics and kinetics.This proof-of-concept study establishes a relationship between the enhanced performance and the multivalent Sn species,and also provides a practicable and scalable avenue for rational engineering high-powered electrocatalysts. | Jun Wu Xue Bai Zhiyu Ren Shichao Du Ziehen Song Lei Zhao Bowen Liu Guiling Wang Honggang Fu | 2021 | Nano Research2021，14，4： | 4 |
| 8 | Genesis of Geothermal Fluid in Typical Geothermal Fields in Western Sichuan,China显示文摘The hydrogeochemical characteristics of geothermal fluids can reveal the genesis of geothermal systems and act as important references for developing and using geothermal resources.This study presents hydrogeochemical processes and thermal cycle mechanisms of typical geothermal fields in Western Sichuan.Based on the geological conditions in Western Sichuan,29 hot springs in three geothermal fields in the Batang and Litang areas were selected for hydrochemical and isotopic(δD andδ18O)analyses.Furthermore,the temperature of the thermal reservoir was calculated and the upflow cooling process of the hot springs was analyzed.Most of the subterranean hot waters in Batang and Litang are of the HCO3-Na hydrochemical type.The ion variation in Batang is primarily affected by water-rock interactions.There is a strong positive correlation between Na+,B?,and Cl?in Litang,suggesting that they have the same material source.The Na+and metaboric acid content is relatively high,which indicates that the groundwater runoff in both areas is relatively long-lasting,with reduced flow velocity;moreover,the metasilicic acid content is relatively high,which supports this conclusion.Both hydrogen and oxygen isotopes plot near the atmospheric precipitation line,indicating that groundwater recharge is functionally obtained from precipitation.The calculated thermal storage temperatures in Batang and Litang were 88–199℃ and 96–154℃,respectively.The proportion of cold water mixing in Batang was 64%–67%,while that in Litang was 60%–68%.According to the calculated results,the initial thermal cycle depth of the Batang area(4540–4780 m)was greater than that of the Litang area(3150–3960 m).The enthalpy of the deep parental geothermal fluid in Batang was 1550 J/g with a Cl^(-)concentration of 37 mg/L,while that in Litang was 2100 J/g with a Cl^(-)concentration of 48 mg/L. | ZHAO Jiayi WANG Guiling ZHANG Cuiyun XING Linxiao LI Man ZHANG Wei | 2021 | Acta Geologica Sinica(English Edition)2021，95，3： | 2 |
| 9 | Isolation and characterization of genomic retrotransposon sequences from octoploid strawberry (Fragaria?×?ananassa Duch.)显示文摘 | Yue Ma Haiyue Sun Guiling Zhao Hongyan Dai Xiuyan Gao He Li Zhihong Zhang | 2008 | Plant Cell Reports2008，，3： | 1 |
| 10 | Imaging Microdomain Ca^2+ in Muscle Cells 显示文摘 | Wang Shiqiang Wei Chaoliang Zhao Guiling | 2004 | Circ Res2004，94，8： | 1 |
| 11 | Efficient Fe based catalyst with nitrogen doped carbon material modification for propane non-oxidative dehydrogenation显示文摘Nitrogen doped carbon species(NC)was introduced into Fe based catalyst and applied into propane non-oxidative dehydrogenation.With characterizations of XRD,TEM and XPS,it was deduced that the NC modification induces the electrons transferred from NC species to Fe species,leading to electron-rich Fe species.Consequently,propane conversion and propene selectivity were increased by suppressing the by-products formation.It was also confirmed that such a modification strategy could be also extended to ones with different supports,iron precursors,and NC precursors. | Yuming Li Qiyang Zhang Xue Yu Guiling Jiang Yajun Wang Guiyuan Jiang Zhen Zhao Chunming Xu State | 2020 | Carbon Resources Conversion2020，3，1： | 1 |
| 12 | Catalytic enantioselective domino oxa-michael/aldol condensations: asymmetric synthesis of benzopyran derivatives显示文摘 | Sundfin H Ibrahem I Zhao guiling | 2007 | Chem Eur J2007，13，2： | 1 |
| 13 | A new catalyst for urea oxidation: NiCo2S4 nanowires modified 3D carbon sponge显示文摘Urea oxidation is a significant reaction for utilizing urea-rich wastewater or human urine as sustainable power sources which can ease the water eutrophication while generate electricity. A direct urea-hydrogen peroxide fuel cell is a new kind of fuel cell employing urea as fuel and hydrogen peroxide as oxidant which possesses a larger cell voltage. Herein, this work tries to promote the kinetics process of urea oxidation by preparing low-cost and high-efficient NiCo2S4 nanowires modified carbon sponge electrode. The carbon sponge used in this work with a similar three-dimensional multi-channel structure to Ni foam, is prepared by carbonizing recycled polyurethane sponge which is also a process of recycling waste. The performance of the prepared catalyst in an alkaline solution is investigated in a three-electrode system.With the introduction of Co element to the catalyst, a reduced initial urea oxidation potential and a high performance are obtained. Furthermore, a direct urea-hydrogen peroxide fuel cell is assembled using the NiCo2S4 nanowires modified carbon sponge anode. Results indicate that the prepared catalyst provides a chance to solve the current problems that hinder the development of urea electrooxidation(high initial urea oxidation potential, low performance, and high electrode costs). | Biaopeng Li Congying Song Jianjun Rong Jing Zhao Hong-En Wang Ping Yang Ke Ye Kui Cheng Kai Zhu Jun Yan Dianxue Cao Guiling Wang | 2020 | Journal of Energy Chemistry2020，29，11： | 1 |
| 14 | Preparation of sintered foam materials by alkali-activated coal fly ash显示文摘 | Yelong Zhao Junwei Ye Xiaobin Lu Mangang Liu Yuan Lin Weitao Gong Guiling Ning | 2009 | Journal of Hazardous Materials2009，，1： | 1 |
| 15 | New insights into the dispersion history and adaptive evolution of taxon Aegilops tauschii in China显示文摘Aegilops tauschii,the wild progenitor of wheat D-genome and a valuable germplasm for wheat improvement,has a wide natural distribution from eastern Turkey to China.However,the phylogenetic relationship and dispersion history of Ae.tauschii in China has not been scientifically clarified.In this study,we genotyped 208 accessions(with 104 in China)using dd RAD sequencing and 55K SNP array,and classified the population into six sublineages.Three possible spreading routes or events were identified,resulting in specific distribution patterns,with four sublineages found in Xinjiang,one in Qinghai,two in Shaanxi and one in Henan.We also established the correlation of SNP-based,karyotypebased and spike-morphology-based techniques to demonstrate the internal classification of Ae.tauschii,and developed consensus dataset with 1245 putative accessions by merging data previously published.Our analysis suggested that eight inter-lineage accessions could be assigned to the putative Lineage 3and these accessions would help to conserve the genetic diversity of the species.By developing the consensus phylogenetic relationships of Ae.tauschii,our work validated the hypothesis on the dispersal history of Ae.tauschii in China,and contributed to the efficient and comprehensive germplasm-mining of the species. | Hao Li Fang Nie Lele Zhu Menghua Mu Ruixiao Fan Jingyao Li Aaqib Shaheen Yifan Liu Can Li Wenjuan Liu Huihui Liang Xinpeng Zhao Shenglong Bai Guanghui Guo Zheng Li Yiheng Hu Yuannian Jiao Jonathan Adams Assaf Distelfeld Guiling Sun Suoping Li Yun Zhou Chun-Peng Song | 2022 | Journal of Genetics and Genomics2022，49，3： | 0 |
| 16 | Grassland Science in a New Era显示文摘Grasslands are one of the major biomes on Earth,covering approximately 25%of the terrestrial planet.Human history is deeply intertwined with grassland biomes,where we,as a natural species,first walked upright 2 million years ago.Today,grassland ecosystems continue to play an important role in people's livelihoods by producing meat and dairy products,providing habitats for biodiversity,and delivering essential ecosystem services such as climate regulation and cultural heritage. | Guiling Zhao Lingli Liu Zeng-Yu Wang Zhenong Jin Jin-Sheng He | 2023 | Fundamental Research2023，3，2： | 0 |
| 17 | Three-Dimensional Reconstruction of Wood Carving Cultural Relics Based on CT Tomography Data显示文摘Three-dimensional reconstruction of wood carving based on CT tomography data is important.In this paper,we propose a novel 3D variational framework for this task,which includes two procedures.First,a fitting approach is applied to a sequence of wood carving images acquired by CT scanner.The regions of interest(ROIs)can be obtained for the second segmentation after fitting.Second,we utilise a 3D TV(total variation)L1 variational model to directly segment the 3D volume of the ROIs.In addition,the TV-L1 model can smooth the volume and gives a clear 3D volume rendering result.By introducing a dual auxiliary variable,the fast primal-dual method is developed to improve the computational efficiency of the 3D TV-L1 model.Extensive experimental results are conducted to demonstrate the performance of the proposed framework. | Guiling Zhao Zongji Deng Jun Shen Zhaowen Qiu Jing Huang | 2017 | 国际计算机前沿大会会议论文集2017，，1： | 0 |
| 18 | Trends in grassland science:Based on the shift analysis of research themes since the early 1900s显示文摘Grasslands,one of the major terrestrial ecosystems,are essential for the maintenance of ecological and production functions;however,they are undergoing extensive degradation.The development and cutting-edge explorations in grassland science are critical to addressing challenges such as climate change and the increasing influence of human activities.To identify research trends in grassland science,latent Dirichlet allocation(LDA)topic modelling was used to conduct an automated content analysis on 123,829 papers available on Web of Science Core Collection from 1900 to 2020.Results from this analysis showed that grassland research has become increasing multidisciplinary,accompanied by a pronounced reduction in the relative frequency of traditional production-oriented research and an increase in the themes focusing on ecological functions and modern technologies.Changes in research activities have been uneven globally,with a significant increase in the number of publications in China and Brazil,which probably reflects an increased support from various governmental agencies in these countries.Additionally,in 2019,China surpassed the United States in terms of the total number of publications.Further,this study identified important topics and emerging challenges in grassland research,such as biodiversity conservation,climate changes,and genetic considerations.Comprehensive improvement of education,research,global cooperation,and funding strategies will be necessary to promote grassland science research on frontier themes and to effectively address the social and environmental challenges in the new era. | Guiling Zhao | 2023 | Fundamental Research2023，3，2： | 0 |
| 19 | Cloning and characterization of two putative seven-transmembrane receptor genes from cotton显示文摘用 cDNA 结束(种族) 的快速的扩大 -PCR, 编码通常认为的 seven-transmembrane 受体(指明的 Gh7TMpR1 和 Gh7TMpR2 ) 的二全身的 cDNAs 从棉花植物被克隆。南部的污点和地点多型性分析的 ApaL1 限制表明 Gh7TMpR1 从祖先的 A 被导出双染色体,当 Gh7TMpR2 从 D 潜水艇染色体时。北污点杂交显示 Gh7TMpR1 和 Gh7TMpR2 在纤维开发的延伸阶段优先地被表示。Gh7TMpR1 蛋白质的多数位于膜结构以内并且显示了分发的一个 punctuate 模式。在分裂酵母的 Gh7TMpR1 的 Overexpression 破坏了极的生长并且引起了圆细胞的形成。这些结果建议 GhT7MpR1 可以在棉花纤维开发起一个关键作用,也许,作为发信号的受体,那涉及控制纤维延伸。 | Peng Gao Piming Zhao Juan Wang Haiyun Wang Guiling Wang Guixian Xia | 2008 | Progress in Natural Science:Materials International2008，18，10： | 0 |
| 20 | Discussion on the Relationship between Different Earthquake Magnitude Scales and the Effect of Seismic Station Sites on Magnitude Estimation显示文摘Based on the earthquake catalog reported by the Chinese digital seismic network in recent years,we select the earthquakes with both surface wave magnitude and local magnitude and fit them into a relationship between the two magnitudes.The systematic difference is found from the formula which has been used for 30 years.Because of a large dynamic range and wide frequency range of the current digital observation system,in addition to a larger number of stations and earthquakes being used compared to before,the relation obtained in this paper seems more reliable.Our calculation shows that there is no significant difference before and after magnitude conversion so we suggest the abandonment of magnitude conversion.The site response of a station consists of amplification at different frequencies.The amplification is equal to about 1 and changes little with frequency at stations located on basement rock,and it is greater than 1 at low frequency ranges and less than 1 at high frequency ranges at stations located on sediment layers.The difference between magnitudes from single station located on sediment layer and the average magnitude from the whole network increases from negative to positive with period.It seems that there is no fixed station correction factor and the station correction method does not work to improve the accuracy and magnitude estimates. | Zhang Hongzhi Diao Guiling Zhao Mingchun Wang Qincai Zhang Xiao Huang Yuan | 2008 | Earthquake Research in China2008，22，1： | 0 |
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