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| 1 | In situ self-assembled organoid for osteochondral tissue regeneration with dual functional units显示文摘The regeneration of hierarchical osteochondral units is challenging due to difficulties in inducing spatial,directional and controllable differentiation of mesenchymal stem cells(MSCs)into cartilage and bone compartments.Emerging organoid technology offers new opportunities for osteochondral regeneration.In this study,we developed gelatin-based microcryogels customized using hyaluronic acid(HA)and hydroxyapatite(HYP),respectively for inducing cartilage and bone regeneration(denoted as CH-Microcryogels and OS-Microcryogels)through in vivo self-assembly into osteochondral organoids.The customized microcryogels showed good cytocompatibility and induced chondrogenic and osteogenic differentiation of MSCs,while also demonstrating the ability to self-assemble into osteochondral organoids with no delamination in the biphasic cartilage-bone structure.Analysis by mRNA-seq showed that CH-Microcryogels promoted chondrogenic differentiation and inhibited inflammation,while OS-Microcryogels facilitated osteogenic differentiation and suppressed the immune response,by regulating specific signaling pathways.Finally,the in vivo engraftment of pre-differentiated customized microcryogels into canine osteochondral defects resulted in the spontaneous assembly of an osteochondral unit,inducing simultaneous regeneration of both articular cartilage and subchondral bone.In conclusion,this novel approach for generating self-assembling osteochondral organoids utilizing tailor-made microcryogels presents a highly promising avenue for advancing the field of tissue engineering. | Zhen Yang Bin Wang Wei Liu Xiaoke Li Kaini Liang Zejun Fan Jiao Jiao Li Yudi Niu Zihao He Hui Li Du Wang Jianjing Lin Yanan Du Jianhao Lin Dan Xing | 2023 | Bioactive Materials2023，，9： | 2 |
| 2 | Auxin signaling module OsSK41-OsIAA10-OsARF regulates grain yield traits in rice显示文摘Auxin is an important phytohormone in plants, and auxin signaling pathways in rice play key roles in regulating its growth, development, and productivity. To investigate how rice grain yield traits are regulated by auxin signaling pathways and to facilitate their application in rice improvement, we validated the functional relationships among regulatory genes such as OsIAA10, OsSK41, and OsARF21 that are involved in one of the auxin(OsIAA10) signaling pathways. We assessed the phenotypic effects of these genes on several grain yield traits across two environments using knockout and/or overexpression transgenic lines.Based on the results, we constructed a model that showed how grain yield traits were regulated by OsIAA10 and OsTIR1, OsAFB2, and OsSK41 and OsmiR393 in the OsSK41-OsIAA10-OsARF module and by OsARF21 in the transcriptional regulation of downstream auxin response genes in the OsSK41-OsIAA10-OsARF module. The population genomic analyses revealed rich genetic diversity and the presence of major functional alleles at most of these loci in rice populations. The strong differentiation of many major alleles between Xian/indica and Geng/japonica subspecies and/or among modern varieties and landraces suggested that they contributed to improved productivity during evolution and breeding. We identified several important aspects associated with the genetic and molecular bases of rice grain and yield traits that were regulated by auxin signaling pathways.We also suggested rice auxin response factor(OsARF) activators as candidate target genes for improving specific target traits by overexpression and/or editing subspecies-specific alleles and by searching and pyramiding the ‘best' gene allelic combinations at multiple regulatory genes in auxin signaling pathways in rice breeding programs. | Fuying Ma Fan Zhang Yu Zhu Dengyong Lan Peiwen Yan Ying Wang Zejun Hu Xinwei Zhang Jian Hu Fuan Niu Mingyu Liu Shicong He Jinhao Cui Xinyu Yuan Ying Yan Shujun Wu Liming Cao Hongwu Bian Jinshui Yang Zhikang Li Xiaojin Luo | 2023 | Journal of Integrative Plant Biology2023，65，7： | 1 |
| 3 | High capacity adsorption of antimony in biomass-based composite and its consequential utilization as battery anode显示文摘Antimony is more than an emerging pollutant in water but a scare resource.In this study,we report an adsorbent with the record capacity so far from the balanced view of Sb(Ⅲ) and Sb(Ⅴ).The composite adsorbent was fabricated by encapsulating hollow Fe_(3)O_(4)nanosphere with the EDTA grafted chitosan,and it has superhigh adsorption capacity of for 657.1 mg/g for Sb(Ⅲ) and 467.3 mg/g for Sb(Ⅴ),respectively.The mechanism study reveals that the adsorption of Sb initializes from the Fe_(3)O_(4),propagates along the chitosan with hydrogen bond,and terminates at the inner sphere complex with the EDTA moiety in the adsorbent.In view of the ultra-high adsorption capacity of the adsorbent,the recovered adsorbent that contains abundant (>36.4%) highly dispersed antimony nanoparticles (600-FCSE-Sb) is applied to Li-ion battery anode after reduction.This article provides a new idea for connecting water treatment and electric energy storage. | Yao Wang Xinyue Zhang Na Ju Hongna Jia Zejun Sun Jiaxing Liang Rongxiu Guo Dun Niu Hong-bin Sun | 2023 | Journal of Environmental Sciences2023，，4： | 1 |
| 4 | Inhibitory effects of HS014 on glutamate release in astrocytes chronically treated with morphine显示文摘Previous studies have confirmed that the number of glial fibrillary acidic protein-positive cells significantly increases during morphine tolerance.However,morphine tolerance is reversed with melanocortin receptor antagonists,and analgesic action is enhanced accordingly.However,these mechanisms remain unclear.In the present study,following addition of morphine to Wistar rat spinal cord astrocytes,glutamate levels in the supernatant significantly increased(P < 0.05).At 30-120 minutes following addition of intervention agent to spinal cord astrocytes,naloxone significantly increased glutamate release in morphine-tolerant model cells(P < 0.05),while melanocortin receptor antagonist HS014 decreased glutamate release(P < 0.05).Additional naloxone and HS014 to astrocytes significantly decreased glutamate release compared with additional naloxone alone(P < 0.01).Results from the present study demonstrated that glutamate release was increased in spinal cord astrocytes co-cultured with morphine.Naloxone increased glutamate release,and HS014 reduced glutamate release. | Haichen Chu Zejun Niu Zhao Yang Xuefeng Zhang | 2010 | Neural Regeneration Research2010，5，19： | 1 |
| 5 | The kinase OsSK41/OsGSK5 negatively regulates amylose content in rice endosperm by affecting the interaction between OsEBP89 and OsBP5显示文摘Amylose content(AC) is the main factor determining the palatability, viscosity, transparency, and digestibility of rice(Oryza sativa)grains. AC in rice grains is mainly controlled by different alleles of the Waxy(Wx) gene. The AP2/EREBP transcription factor OsEBP89 interacts with the MYC-like protein OsBP5 to synergistically regulate the expression of Wx.Here, we determined that the GLYCOGEN SYNTHASE KINASE 5(OsGSK5, also named SHAGGY-like kinase 41 [OsSK41]) inhibits the transcriptional activation activity of OsEBP89 in rice grains during amylose biosynthesis. The loss of OsSK41 function enhanced Wx expression and increased AC in rice grains. By contrast, the loss of function of OsEBP89 reduced Wx expression and decreased AC in rice grains. OsSK41 interacts with OsEBP89 and phosphorylates four of its sites(Thr-28,Thr-30, Ser-238, and Thr-257), which makes OsEBP89 unstable and attenuates its interaction with OsBP5. Wx promoter activity was relatively weak when regulated by the phosphomimicvariantOsEBP89E–OsBP5but relatively strong when regulated by the nonphosphorylatable variant OsEBP89A–OsBP5.Therefore, OsSK41-mediated phosphorylation of OsEBP89 represents an additional layer of complexity in the regulation of amylose biosynthesis during rice grain development. In addition, our findings provide four possible sites for regulating rice grain AC via precise gene editing. | Zejun Hu Fuan Niu Peiwen Yan Kai Wang Lixia Zhang Ying Yan Yu Zhu Shiqing Dong Fuying Ma Dengyong Lan Siwen Liu Xiaoyun Xin Ying Wang Jinshui Yang Liming Cao Shujun Wu Xiaojin Luo | 2023 | Journal of Integrative Plant Biology2023，65，7： | 0 |
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