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| 1 | Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures显示文摘 | Bin-Kuan Chou Prashant Mali Xiaosong Huang Zhaohui Ye Sarah N Dowey Linda MS Resar Chunlin Zou Alex Zhang Jay Tong Linzhao Cheng | 2011 | Cell Research2011，21，3： | 38 |
| 2 | Down.regulation of E.cadherin enhances prostate cancer chemoresistance via Notch signaling显示文摘Background:The chemoresistance of prostate cancer(PCa)is invariably associated with the aggressiveness and metastasis of this disease.New emerging evidence indicates that the epithelial-to-mesenchymal transition(EMT)may play pivotal roles in the development of chemoresistance and metastasis.As a hallmark of EMT,E-cadherin is suggested to be a key marker in the development of chemoresistance.However,the molecular mechanisms underlying PCa chemoresistance remain unclear.The current study aimed to explore the association between EMT and chemoresistance in PCa as well as whether changing the expression of E-cadherin would affect PCa chemoresistance.Methods:Parental PC3 and DU145 cells and their chemoresistant PC3-Tx R and DU145-Tx R cells were analyzed.PC3-Tx R and DU145-Tx R cells were transfected with E-cadherin-expressing lentivirus to overexpress E-cadherin;PC3 and DU145 cells were transfected with small interfering RNA to silence E-cadherin.Changes of EMT phenotype-related markers and signaling pathways were assessed by Western blotting and quantitative real-time polymerase chain reaction.Tumor cell migration,invasion,and colony formation were then evaluated by wound healing,transwell,and colony formation assays,respectively.The drug sensitivity was evaluated using MTS assay.Results:Chemoresistant PC3-Tx R and DU145-Tx R cells exhibited an invasive and metastatic phenotype that associated with EMT,including the down-regulation of E-cadherin and up-regulation of Vimentin,Snail,and N-cadherin,comparing with that of parental PC3 and DU145 cells.When E-cadherin was overexpressed in PC3-Tx R and DU145-Tx R cells,the expression of Vimentin and Claudin-1 was down-regulated,and tumor cell migration and invasion were inhibited.In particular,the sensitivity to paclitaxel was reactivated in E-cadherin-overexpressing PC3-Tx R and DU145-Tx R cells.When E-cadherin expression was silenced in parental PC3 and DU145 cells,the expression of Vimentin and Snail was up-regulated,and,particularly,the sensitivity to paclitaxel was decreased.Interestingly,Notch-1 expression was up-regulated in PC3-Tx R and DU145-Tx R cells,whereas the E-cadherin expression was down-regulated in these cells comparing with their parental cells.The use ofγ-secretase inhibitor,a Notch signaling pathway inhibitor,significantly increased the sensitivity of chemoresistant cells to paclitaxel.Conclusion:The down-regulation of E-cadherin enhances PCa chemoresistance via Notch signaling,and inhibiting the Notch signaling pathway may reverse PCa chemoresistance. | Wenchu Wang Lihui Wang Atsushi Mizokami Junlin Shi Chunlin Zou Jinlu Dai Evan T. Keller Yi Lu Jian Zhang | 2017 | Chinese Journal of Cancer2017，36，3： | 16 |
| 3 | Labeling of cynomolgus monkey bone marrow-derived mesenchymal stem cells for cell tracking by multimodality imaging显示文摘Recently,transplantation of allogeneic and autologous cells has been used for regenerative medicine.A critical issue is monitoring migration and homing of transplanted cells,as well as engraftment efficiency and functional capability in vivo.Monitoring of superparamagnetic iron oxide(SPIO) particles by magnetic resonance imaging(MRI) has been used in animal models and clinical settings to track labeled cells.A major limitation of MRI is that the signals do not show biological characteristics of transplanted cells in vivo.Bone marrow mesenchymal stem cells(MSCs) have been extensively investigated for their various therapeutic properties,and exhibit the potential to differentiate into cells of diverse lineages.In this study,cynomolgus monkey MSCs(cMSCs) were labeled with Molday ION Rhodamine-BTM(MIRB),a new SPIO agent,to investigate and characterize the biophysical and MRI properties of labeled cMSCs in vitro and in vivo.The results indicate that MIRB is biocompatible and useful for cMSCs labeling and cell tracking by multimodality imaging.Our method is helpful for detection of transplanted stem cells in vivo,which is required for understanding mechanisms of cell therapy. | REN ZhenHua WANG JiaYin ZOU ChunLin GUAN YunQian ZHANG Yu Alex | 2011 | Science China(Life Sciences)2011，54，11： | 9 |
| 4 | Inducible regulation of GDNF expression in human neural stem cells显示文摘Glial cell derived neurotrophic factor (GDNF) holds promises for treating neurodegenerative diseases such as Parkinson's disease. Human neural stem cells (hNSCs) have proved to be a suitable cell delivery vehicle for the safe and efficient introduction of GDNF into the brain. In this study, we used hNSCs-infected with a lentivirus encoding GDNF and the hygromycin resistance gene as such vehicles. A modified tetracycline operator 7 (tetO7) was inserted into a region upstream of the EF1- promoter to drive GDNF expression. After hygromycin selection, hNSCs were infected with a lentivirus encoding a KRAB-tetracycline repressor fusion protein (TTS). TTS bound to tetO7 and suppressed the expression of GDNF in hNSCs. Upon administration of doxycycline (Dox) the TTS-tetO7 complex separated and the expression of GDNF resumed. The hNSCs infected with GDNF expressed the neural stem cell specific markers, nestin and sox2, and exhibited no significant change in proliferation rate. However, the rate of apoptosis in hNSCs expressing GDNF was lower compared with normal NSCs in response to actinomycin treatment. Furthermore, a higher percentage of Tuj-1 positive cells were obtained from GDNF-producing NSCs under conditions that induced differentiation compared to control NSCs. The inducible expression of GDNF in hNSCs may provide a system for the controllable delivery of GDNF in patients with neurodegenerative diseases. | WANG ShuYan REN Ping GUAN YunQian ZOU ChunLin FU LinLin ZHANG Yu | 2013 | Science China(Life Sciences)2013，56，1： | 5 |
| 5 | Characterizing the induction of diabetes in juvenile cynomolgus monkeys with different doses of streptozotocin显示文摘Juvenile(2-3 years old) cynomolgus monkeys are frequently used as recipients in non-human primate islet transplantation studies.The aim of this study was to examine the effects of different doses of streptozotocin(STZ),and find the optimal dose for inducing diabetes in these monkeys.Fifteen juvenile(2-3 years old) cynomolgus monkeys were separated into three groups and administered with different doses of STZ(100,68 or 60 mg kg-1).Basal and glucose-stimulated blood glucose,insulin,and C-peptide levels,as well as body weights were monitored.Hepatic and renal function tests and pancreatic immunohistochemistry were performed before and after STZ treatment.Monkeys treated with both 100 and 68 mg kg-1 of STZ exhibited continuous hyperglycemia,which coincided with a nearly complete loss of islet--cells.Two monkeys received 60 mg kg-1 of STZ,but only one became completely diabetic.During the first week following STZ treatment,hepatic and renal function slightly increased in these three groups.However,24 hours post-STZ,serum total bile acid levels were significantly increased in monkeys treated with 100 mg kg-1 than those treated with 68 mg kg-1 of STZ(P<0.05).These data suggest that 100 mg kg-1 and 68 mg kg-1 of STZ can safely induce diabetes in cynomolgus monkeys aged 2-3 years,but 68 mg kg-1 of STZ,rather than 100 mg kg-1 of STZ,may be more appropriate for inducing diabetes in these monkeys.Furthermore,body surface area,rather than body weight,was a more reliable determinant of dosage,where 700 mg m-2 of STZ should be the lower limit for inducing diabetes in juvenile monkeys. | ZOU ChunLin WANG JiaYin WANG ShuYan HUANG Fen REN ZhenHua CHEN ZhiGuo ZHANG YU | 2012 | Science China(Life Sciences)2012，55，3： | 3 |
| 6 | Prostate cancer tends to metastasize in the bone-mimicking microenvironment via activating NF-κB signaling显示文摘Prostate cancer preferentially metastasizes to the bone. However, the underlying molecular mechanisms are still unclear. To explore the effects of a bone-mimicking microenvironment on PC3 prostate cancer cell growth and metastasis, we used osteoblast differentiation medium(ODM; minimal essential medium alpha supplemented with L-ascorbic acid) to mimic the bone microenvironment. PC3 cells grown in ODM underwent epithelial-mesenchymal transition and showed enhanced colony formation, migration, and invasion abilities compared to the cells grown in normal medium. PC3 cells grown in ODM showed enhanced metastasis when injected in mice. A screening of signaling pathways related to invasion and metastasis revealed that the NF-κB pathway was activated, which could be reversed by Bay 11-7082, a NF-κB pathway inhibitor. These results indicate that the cells in different culture conditions manifested significantly different biological behaviors and the NF-κB pathway is a potential therapeutic target for prostate cancer bone metastasis. | Haibo Tong Chunlin Zou Siyuan Qin Jie Meng Evan T.Keller Jian Zhang Yi Lu | 2018 | The Journal of Biomedical Research2018，32，5： | 3 |
| 7 | E-cadherin表达下调激活Notch信号通路增强前列腺癌化疗耐药的机制研究显示文摘背景与目的前列腺癌(prostate cancer,PCa)化疗耐药与其侵袭性和转移性密切相关。最新研究显示上皮细胞–间充质转化(epithelial-to-mesenchymal transition,EMT)可能在前列腺癌化疗耐药和转移中发挥重要作用。因此,作为EMT的标志物,E-cadherin被认为有可能与肿瘤化疗耐药有重要关系。然而,PCa化疗耐药的分子机制尚不清楚。本研究旨在探讨EMT与PCa化疗耐药的关系,以及E-cadherin表达变化是否影响PCa化疗耐药。方法以亲代PC3和DU145细胞及其化疗耐药PC3-TxR和DU145-TxR细胞作为研究对象。用E-cadherin过表达的慢病毒感染PC3-TxR和DU145-TxR细胞,使E-cadherin过表达;同时,用小干扰RNA转染PC3和DU145细胞,使E-cadherin表达沉默。采用蛋白免疫印迹和实时定量聚合酶链反应分析EMT相关标志物及信号通路的变化。分别通过划痕、transwell和克隆形成实验检测肿瘤细胞的迁移、侵袭和克隆形成能力。采用MTS法检测细胞对紫杉醇的药物敏感性。结果与亲代PC3和DU145细胞相比,化疗耐药PC3-TxR和DU145-TxR细胞表现出EMT相关的标志物表达改变,包括E-cadherin表达下调和Vimentin、Snail与N-cadherin表达上调。当E-cadherin在PC3-TxR和DU-145-TxR细胞中过表达时,Vimentin和Caldun-1表达下调,肿瘤细胞的迁移和侵袭受到抑制。特别是E-cadherin过表达以后,PC3-TxR和DU145-TxR细胞对紫杉醇的敏感性被重新激活。并且,在亲代PC3和DU145细胞中沉默E-cadherin表达后,Vimentin和Snail表达上调,对紫杉醇的敏感性降低。有趣的是,与亲代PC3和DU145细胞相比,PC3-TxR和DU145-TxR细胞中Notch-1表达上调,而E-cadherin在这些细胞中的表达下调。我们的研究还显示,Notch信号通路抑制剂γ-分泌酶抑制剂可显著增加化疗耐药细胞对紫杉醇的敏感性。结论 E-cadherin下调可以激活Notch信号通路,从而增强PCa化疗耐药,抑制Notch信号通路可能逆转PCa化疗耐药。 | Wenchu Wang Lihui Wang Atsushi Mizokami Junlin Shi Chunlin Zou Jinlu Dai Evan T.Keller Yi Lu Jian Zhang | 2019 | 癌症2019，38，3： | 1 |
| 8 | Low Incidence of DNA Sequence Variation in Human Induced Pluripotent Stem Cells Generated by Nonintegrating Plasmid Expression显示文摘 | Linzhao Cheng Nancy F. Hansen Ling Zhao Yutao Du Chunlin Zou Frank X. Donovan Bin-Kuan Chou Guangyu Zhou Shijie Li Sarah N. Dowey Zhaohui Ye Settara C. Chandrasekharappa Huanming Yang James C. Mullikin P. Paul Liu | 2012 | Cell Stem Cell2012，，3： | 1 |
| 9 | The underlying mechanism of variety–water–nitrogen–stubble damage interactions on yield formation in ratoon rice with low stubble height under mechanized harvesting显示文摘Agronomic measures are the key to promote the sustainable development of ratoon rice by reducing the damage from mechanical crushing to the residual stubble of the main crop, thereby mitigating the impact on axillary bud sprouting and yield formation in ratoon rice. This study used widely recommended conventional rice Jiafuzhan and hybrid rice Yongyou 2640 as the test materials to conduct a four-factor block design field experiment in a greenhouse of the experimental farm of Fujian Agricultural and Forestry University, China from 2018 to 2019.The treatments included fertilization and no fertilization, alternate wetting and drying irrigation and continuous water flooding irrigation, and plots with and without artificial crushing damage on the rice stubble. At the same time, a 13C stable isotope in-situ detection technology was used to fertilize the pot experiment. The results showed significant interactions among varieties, water management, nitrogen application and stubble status.Relative to the long-term water flooding treatment, the treatment with sequential application of nitrogen fertilizer coupled with moderate field drought for root-vigor and tiller promotion before and after harvesting of the main crop, significantly improved the effective tillers from low position nodes. This in turn increased the effective panicles per plant and grains per panicle by reducing the influence of artificial crushing damage on rice stubble and achieving a high yield of the regenerated rice. Furthermore, the partitioning of 13C assimilates to the residual stubble and its axillary buds were significantly improved at the mature stage of the main crop, while the translocation rate to roots and rhizosphere soil was reduced at the later growth stage of ratooning season rice. This was triggered by the metabolism of hormones and polyamines at the stem base regulated by the interaction of water and fertilizer at this time. We therefore suggest that to achieve a high yield of ratoon rice with low stubble height under mechanized harvesting, the timely application of nitrogen fertilizer is fundamental,coupled with moderate field drying for root-vigor preservation and tiller promotion before and after the mechanical harvesting of the main crop. | Jingnan Zou Ziqin Pang Zhou Li Chunlin Guo Hongmei Lin Zheng Li Hongfei Chen Jinwen Huang Ting Chen Hailong Xu Bin Qin Puleng Letuma Weiwei Lin Wenxiong Lin | 2024 | Journal of Integrative Agriculture2024，23，3： | 0 |
| 10 | Single-cell transcriptomics reveals cell type diversity of human prostate显示文摘Extensive studies have been performed to describe the phenotypic changes occurring during malignant transformation of the prostate.However,the cell types and associated changes that contribute to the development of prostate diseases and cancer remain elusive,largely due to the heterogeneous composition of prostatic tissues.Here,we conduct a comprehensive evaluation of four human prostate tissues by singlecell RNA sequencing(sc RNA-seq)to analyze their cellular compositions.We identify 18 clusters of cell types,each with distinct gene expression profiles and unique features;of these,one cluster of epithelial cells(Ep)is found to be associated with immune function.In addition,we characterize a special cluster of fibroblasts and aberrant signaling changes associated with prostate cancer(PCa).Moreover,we provide insights into the epithelial changes that occur during the cellular senescence and aging.These results expand our understanding of the unique functional associations between the diverse prostatic cell types and the contributions of specific cell clusters to the malignant transformation of prostate tissues and PCa development. | Yang Chen Peng Zhang Jinling Liao Jiwen Cheng Qin Zhang Tianyu Li Haiying Zhang Yonghua Jiang Fangxing Zhang Yanyu Zeng Linjian Mo Haibiao Yan Deyun Liu Qinyun Zhang Chunlin Zou Gong-Hong Wei Zengnan Mo | 2022 | Journal of Genetics and Genomics2022，49，11： | 0 |
| 11 | Multifunctional electroactive bio-adhesive for robustly-integrated wound therapy and postoperative wound-status warning and assessment显示文摘Wound abnormalities such as secondary wound laceration and inflammation are common postoperative health hazards during clinical procedures.The continuous treatment,healing induction,and real-time visualization of wound status and complications,including wound re-tearing,inflammation,and morphology,are key focal points for comprehensive healthcare.Herein,an on-demand quadruple energy dissipative strategy was proposed for the nanoengineering of a physically and chemically synergistic double-layer gelatin-based bio-adhesive(DLGel)by combining a multi-network adhesive layer and a versatile electroactive energy dissipative layer based on contrivable interlocking micro-pillar arrays and crosslinked polymer chains.The subtly multiple energy dissipation designs enable DLGel with robust adhesive strength to omnipotently wet and dynamic tissue,providing a basis for reliable wound closure.DLGel achieves comprehensive wound-healing induction through electrical stimulation and possesses reversible underwater light/thermal adhesion,excellent hemostatic performance,outstanding antimicrobial properties,and self-repair capability.Furthermore,a novel deep-learning strategy is creatively established to respond to mechanical deformation due to wound anomalies.This strategy translates biological information into visual graphics,providing real-time early warning and assessment of postoperative wound-abnormality/-morphology,such as laceration,inflammation,and necrosis.Therefore,DLGel and its associated signal collection and processing protocol enable the integration of reliable wound closure,wound healing,and real-time postoperative wound-status warning and assessment within the unobservable and undetectable“black box”regions in a context of non-clinical comprehensive therapy. | Ouyang Yue Xuechuan Wang Mengdi Hou Siwei Sun Manhui Zheng Xiaoliang Zou Zhongxue Bai Chunlin Liu Xinhua Liu | 2024 | Nano Research2024，17，5： | 0 |
| 12 | A Niche-Based Evolutionary Tabu Search Algorithm for Optimal Coordination of Protection Relays in Power Networks显示文摘In this paper, refusal of operation and the degree of constraint violation are considered as one part of the objective function for optimizing the protection relay setting coordination of over current relays in power systems; a new expression of objec- tive function in this optimization problem is proposed. To combine the advantages of both Tabu search (TS) and evolutionary algo- rithms (EAs), a new niche-based evolutionary Tabu search algo- rithm (named NETS) is presented to solve this optimization prob- lem. Moreover, in two cases of power networks, comparisons be- tween NETS and two published algorithms are given. Experimen- tal results show the expression of the objective function for pro- tection relay setting is feasible and reasonable, and the proposed algorithm NETS exhibits a good performance. | YUAN Rongxiang ZOU Xiufen XU Chunlin | 2009 | Wuhan University Journal of Natural Sciences2009，14，3： | 0 |
| 13 | 寨卡病毒基因组RNA结构的综合分析揭示了病毒感染性的关键决定因显示文摘文章简介寨卡病毒(ZIKV)通过蚊虫叮咬传播,对人类健康有严重的威胁。寨卡病毒包括两个株系:一个比较古老的非洲株系,另一种是当前流行的亚洲株系(又称美洲株系)。理解流行株系和非流行株系的基因组差异在病毒传播过程中的作用,对于深入了解寨卡病毒的机制十分重要。 | 李盼 魏逸凡 梅淼 唐磊 Lei Sun Wenze Huang Jianyu Zhou Chunlin Zou Shaojun Zhang 秦成峰 姜涛 戴剑锋 谭旭 张强锋 | 2019 | 科学新闻2019，0，2： | 0 |
| 14 | Classification,Protection and Sustainable Utilization of Living Heritage Bridges Based on Value Evaluation:A Case Study of Hangzhou显示文摘Living heritage bridge is a part of living heritage,which not only plays a traffic function in today's society,but also has historical,cultural and artistic value of heritage bridge,and has gradually become an important part of world heritage protection.In this paper,the living heritage bridges in Hangzhou are taken as samples,which are divided into four categories:“use value”,“landscape value”,“social and cultural value”and“historical value”.Different protection schemes are put forward for different types of bridges,aiming at classifying and protecting the living heritage bridges in Hangzhou and providing reference for the protection of living heritage bridges in China. | ZHANG Chunlin ZOU Wanting | 2023 | Journal of Landscape Research2023，15，1： | 0 |
| 15 | Production Performance of Different Generations of Muchuan Blackbone Chicken显示文摘In order to formulate feasible conservation measures and develop and utilize Muchuan black-bone chicken rationally,two generations of Muchuan black-bone chicken were bred,and their production performance and slaughter traits( at the age of 300 d) were compared. The results showed that the reproductive performance,slaughter performance and body size of the Muchuan black-bone chicken of Generation 1( G_1)were all improved compared with those of the Generation 0( G_0)( P <0. 05). At the age of 300 d,the live weight,carcass weight,and leg muscle weight of the G_1 were significantly higher than those of the G_0( P <0. 05); and the chest width of the cocks of the G_1 was significantly higher than that of the G_0( P <0. 05). It suggests that after rigorous selection and utilization of modern poultry breeding techniques,the reproductive performance,slaughter performance and survival rate of Muchuan black-bone chicken can be improved significantly. | Mohan QIU Zengrong ZHANG Xiaosong JIANG Huarui DU Qingyun LI Chunlin YU Xia XIONG Lihe ZOU Chaowu YANG | 2018 | Asian Agricultural Research2018，10，8： | 0 |
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