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| 1 | Germline acquisition of Cas9/RNA-mediated gene modifications in monkeys显示文摘 | Yongchang Chen Yiqiang Cui Bin Shen Yuyu Niu Xiaoyang Zhao Lei Wang Jianying Wang Wei Li Qi Zhou Weizhi Ji Jiahao Sha Xingxu Huang | 2015 | Cell Research2015，25，2： | 8 |
| 2 | Induction of matrix metalloproteinase-9 and-2 activity in mouse blastocyst by fibronectin-integrin interaction显示文摘Fibronectin, a major extracellular matrix, plays an important role in embryo implantation by mediating embryo adhesion and outgrowth. In this work, mouse blastocysts produced pro-matrix metalloproteinase-9, pro-matrix metalloproteinase-2 and 64 ku matrix metalloproteinase-2 when they were co-cultured with fibronectin. In contrast, mouse blastocysts did not produce these proteinases without fibronectin. Focal adhesion kinase is a fundamental molecule of integrin signaling pathway and its antisense oligodeoxynucleiotide inhibited blastocyst matrix metalloproteinases expression induced by fibronectin. The results indicated that fibronectin triggered matrix metalloproteinase-9 and -2 expression in mouse blastocyst through its integrin receptors and subsequent signaling pathway, which enhanced the synchronization of blastocyst invasiveness and uterine receptivity and ensured the accuracy of events relative to implantation in timing and spatiality. | TIE Guodong CAO Yujing ZHAO Xingxu Duan Enkui | 2000 | Chinese Science Bulletin2000，45，14： | 7 |
| 3 | Generation of fertile offspring from Kitw/Kitwv mice through differentiation of gene corrected nuclear transfer embryonic stem cells显示文摘 | Yan Yuan Quan Zhou Haifeng Wan Bin Shen Xuepeng Wang Mei Wang Chunjing Feng Mingming Xie Tiantian Gu Tao Zhou Rui Fu Xingxu Huang Qi Zhou Jiahao Sha Xiao-Yang Zhao | 2015 | Cell Research2015，25，7： | 7 |
| 4 | Generation and characterization of stable pig pregastrulation epiblast stem cell lines显示文摘Pig epiblast-derived pluripotent stem cells are considered to have great potential and broad prospects for human therapeutic model developme nt and livestock breeding.Despite on going attempts since the 1990s,no stably defined pig epiblast-derived stem cell line has bee n established.Here,guided by in sights from a large-scale sin gle-cell tran scriptome an alysis of pig embryos from embryonic day(E)0 to E14,specifically,the tracing of pluripotency changes during epiblast development,we developed an in vitro culture medium for establishing and maintaining stable pluripotent stem cell lines from pig E10 pregastrulation epiblasts(pgEpiSCs).Enabled by chemical inhibition of WNT-related signaling in combination with growth factors in the FGF/ERK,JAK/STAT3,and Activin/Nodal pathways,pgEpiSCs maintain their pluripotency transcriptome features,similar to those of E10 epiblast cells,and normal karyotypes after more than 240 passages and have the potential to differentiate into three germ layers.Strikingly,ultradeep in situ Hi-C analysis revealed functional impacts of chromatin 3D-spatial associations on the transcriptional regulation of pluripote ncy marker genes in pgEpiSCs.I n practice,we con firmed that pgEpiSCs readily tolerate at least three rounds of successive gene editi ng and gene rated cloned gen e-edited live piglets.Our findings deliver on the long-a nticipated promise of pig pluripote nt stem cells and open new avenues for biological research,animal husbandry,and regenerative biomedicine. | Minglei Zhi Jinying Zhang Qianzi Tang Dawei Yu Shuai Gao Dengfeng Gao Pengliang Liu Jianxiong Guo Tang Hai Jie Gao Suying Cao Zimo Zhao Chongyang Li Xiaogang Weng Mengnan He Tianzhi Chen Yingjie Wang Keren Long Deling Jiao Guanglei Li Jiaman Zhang Yan Liu Yu Lin Daxin Pang Qianqian Zhu Naixin Chen Jingjing Huang Xinze Chen Yixuan Yao Jingcang Yang Zicong Xie Xianya Huang Mengxin Liu Ran Zhang Qiuyan Li Yiliang Miao Jianhui Tian Xingxu Huang Hongsheng Ouyang Bofeng Liu Wei Xie Qi Zhou Zhonghua Liu Caihong Zheng Mingzhou Li Jianyong Han | 2022 | Cell Research2022，32，4： | 3 |
| 5 | IGF-Ⅱ and IGFBP-1 reversely regulate blastocyst implantation in mouse显示文摘Insulin-like growth factor (IGF)-Ⅱ and IGF binding protein (IGFBP)-1, members of IGF family, are important in the cyclic development of endometrium and the blastocyst implantation. In the present study, the indirect immunofluorescence showed that IGF- Ⅱ and IGFBP-1 were specifically expressed at the maternal-fetal interface. In a co-culture system, IGF-Ⅱ significantly enhanced the attachment and outgrowth of the blastocyst on monolayer of uterine epithelial cells, while IGFBP-1 did not affect the blastocyst attachment, but markedly inhibited the blastocyst outgrowth. The results of zymography showed that IGF- Ⅱ enhanced the activities of MMP-2 and MMP-9, while IGFBP-1 did not affect the activities of MMP-2 and MMP-9. In conclusion, the equilibrium between the invasion of tro-phoblast and the inhibition of deciduas may be regulated by the interaction between the IGF- Ⅱ -expressing invading cy-totrophoblast and maternal deciduas-derived IGFBP-1. | TIAN Yongqiang, TIE Guodong, CAO Yujing, ZHAO Xingxu & DUAN Enkui1. State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China 2. College of Vet Medicine, Gansu Agricultural University, Lanzhou 730070, China | 2002 | Chinese Science Bulletin2002，47，21： | 3 |
| 6 | Physiological and genome-wide gene expression analyses of cold-induced leaf rolling at the seedling stage in rice(Oryza sativa L.)显示文摘Leaf rolling and discoloration are two chilling-injury symptoms that are widely used as indicators for the evaluation of cold tolerance at the seedling stage in rice. However, the difference in cold-response mechanisms underlying these two traits remains unknown. In the present study, a cold-tolerant rice cultivar, Lijiangxintuanheigu, and a cold-sensitive cultivar, Sanhuangzhan-2, were subjected to low-temperature treatments and physiolog-ical and genome-wide gene expression analyses were conducted. Leaf rolling occurred at temperatures lower than 11℃, whereas discoloration appeared at moderately low temperatures such as 13℃. Chlorophyll contents in both cultivars were significantly decreased at 13℃, but not altered at 11℃. In contrast, the relative water content and relative electrolyte leakage of both cultivars decreased significantly at 11℃, but did not change at 13℃. Expression of genes associated with calcium signaling and abscisic acid (ABA) degradation was significantly altered at 11℃ in comparison with 25℃ and 13℃. Numerous genes in the DREB, MYB, bZIP, NAC, Zinc finger, bHLH, and WRKY gene families were differentially expressed. Many aquaporin genes and the key genes in trehalose and starch synthesis were down regulated at 11℃ in comparison with 25℃ and 13℃. These results suggest that the two chilling injury symptoms are temperature-specific and are controlled by different mechanisms. Cold-induced leaf rolling is associated with calcium and ABA signaling pathways and is regulated by multiple transcriptional regulators. The suppression of aquaporin genes and reduced accumulation of soluble sugars under cold stress results in a reduction in cellular water potential and consequently leaf rolling. | Jingfang Dong Junliang Zhao Shaohong Zhang Tifeng Yang Qing Liu Xingxue Mao Hua Fu Wu Yang Bin Liu | 2019 | The Crop Journal2019，7，4： | 1 |
| 7 | Regulation of mouse blastocyst adhesion,outgrowth and matrix metalloproteinase-2 by focal adhesion kinase显示文摘The interaction of extracellular matrix-integrin markedly influences the adhesion, outgrowth, differentiation and expression of serine proteinases by the blastocyst, so it is regarded as a vital factor in blastocyst implantation. Al- though the mechanism of extracellular interactions between extracellular matrix and integrins has been well elucidated, the roles of the signaling molecules in the extracellular ma- trix-integrin signal transduction pathway in blastocyst im- plantation are unknown. This limits the understanding of blastocyst implantation and ECM-integrin signal transduc- tion pathway. In the present study, in vitro blastocyst culture and indirect immunocytochemistry, matrix metallopro- teinases (MMPs) zymography and antisense oligodeoxynu- cleotide (ODN) were used to investigate the expression of a fundamental molecule of integrin-dependent signal trans- duction pathways, focal adhesion kinase (FAK), in mouse blastocysts and its influence on mouse blastocyst adhesion, outgrowth and MMP-2. The results showed that mouse blas- tocysts expressed FAK. FAK protein was clustered in the peripheral migrating trophoblast cells and dispersed in the central area of blastocyst outgrowth. Fibronectin triggered pro-MMP-2 and 64 kD MMP-2 activities. The antisense ODN to FAK attenuated pro-MMP-2 and 64 kD MMP-2 activities which decreased abruptly and tended to disappear with increasing concentrations of the antisense ODN. Both mouse blastocyst adhesion and outgrowth on fibronectin were also influenced by the antisense ODN. Up to 20 mg/mL of the antisense ODN concentration, the adhesion and out- growth rates were decreased in a dose-dependent manner. The results indicated that FAK influenced mouse blastocyst adhesion, outgrowth and MMP-2 activity by intracellular signal transduction. In other words, FAK regulates mouse implantation in terms of blastocyst adhesive and invasive abilities. | TIE Guodong TIAN Yongqiang CHEN Shuyi CAO Yujing LIU Zelong DUAN Enkui ZHAO Xingxu | 2003 | Chinese Science Bulletin2003，48，5： | 0 |
| 8 | Association of self-reported health with alcohol consumption: a study conducted among Chinese and Canadian middle-aged and older adults显示文摘There has been lack of studies that examine the relationship between self-rated health(SRH)and alcohol consumption,particularly in middle-aged and older adult populations.This study aims to investigate association of SRH status with alcohol consumption in middle-aged and older adult populations.The study is a cross-sec-tional study design that was based on the China Health and Retired Longitudinal study(CHARLS)and the Canadian Alcohol and Drug Monitoring Survey(CAD-UMS)and the Canadian Alcohol,Tobacco and Drug Survey(CTADS).Measure of health is SRH status and self-reported alcohol use.Multinomial logistic regres-sion was performed to model the data and the odds ratio of self-reported excel-lent,very good,good and fair(versus poor)for current and former drinkers versus nondrinkers and current drinkers versus former drinkers was estimated in models after adjusting for covariates.This study found that in both Chinese and Canadian adult populations,current drinkers reported better health status than former drink-ers;lifetime nondrinkers reported better health status than former drinkers.Current drinkers reported better health status than nondrinkers.Canadian moderate drinkers(1.3-44 g per day)tended to report better health compared with occasional drinkers and Chinese heavy drinkers(45+grams per day)reported better health status com-pared with occasional drinkers in both populations.This study found current drink-ers tended to have better health status than former drinker and lifetime nondrinkers in adult populations.Nondrinkers tended to report better health status than former drinkers.Low and medium drinkers reported better health status. | Jinhui Zhao Hongpeng Sun Yana Ma Xingxue Cheng | 2020 | China Population and Development Studies2020，4，4： | 0 |
| 9 | Direct evidence of CRISPR-Cas9-mediated mitochondrial genome editing显示文摘Pathogenic mitochondrial DNA(mtDNA)mutations can cause a variety of human diseases.The recent development of genome-editing technologies to manipulate mtDNA,such as mitochondria-targeted DNA nucleases and base editors,offer a promising way for curing mitochondrial diseases caused by mtDNA mutations.The CRISPR-Cas9 system is a widely used tool for genome editing;however,its application in mtDNA editing is still under debate.In this study,we developed a mito-Cas9 system by adding the mitochondria-targeted sequences and 30 untranslated region of nuclear-encoded mitochondrial genes upstream and downstream of the Cas9 gene,respectively. | Rui Bi Yu Li Min Xu Quanzhen Zheng Deng-Feng Zhang Xiao Li Guolan Ma Bolin Xiang Xiaojia Zhu Hui Zhao Xingxu Huang Ping Zheng Yong-Gang Yao | 2022 | The Innovation2022，3，6： | 0 |
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