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| 1 | Over-expression of WOX1 Leads to Defects in Meristem Development and Polyamine Homeostasis in Arabidopsis显示文摘In plants,the meristem has to maintain a separate population of pluripotent cells that serve two main tasks,i.e.,self-maintenance and organ initiation,which are separated spatially in meristem.Prior to our study,WUS and WUS-like WOX genes had been reported as essential for the development of the SAM.In this study,the consequences of gain of WOX1 function are described.Here we report the identification of an Arabidopsis gain-of-function mutant wox1-D,in which the expression level of the WOX1(WUSCHEL HOMEOBOX 1) was elevated and subtle defects in meristem development were observed.The wox1-D mutant phenotype is dwarfed and slightly bushy,with a smaller shoot apex.The wox1-D mutant also produced small and dark green leaves,and exhibited a failure in anther dehiscence and male sterility.Molecular evidences showed that the transcription of the stem cell marker gene CLV3 was down-regulated in the meristem of wox1-D but accumulated in the other regions,i.e.,in the root-hypocotyl junction and at the sites for lateral root initiation.The fact that the organ size and cell size in leaves of wox1-D are smaller than those in wild type suggests that cell expansion is possibly affected in order to have partially retarded the development of lateral organs,possibly through alteration of CLV3 expression pattern in the meristem.An S-adenosylmethionine decarboxylase(SAMDC) protein,SAMDC1,was found able to interact with WOX1 by yeast two-hybrid and pull-down assays in vitro.HPLC analysis revealed a significant reduction of polyamine content in wox1-D.Our results suggest that WOX1 plays an important role in meristem development in Arabidopsis,possibly via regulation of SAMDC activity and polyamine homeostasis,and/or by regulating CLV3 expression. | Yanxia Zhang Renhong Wu Genji Qin Zhangliang Chen Hongya Gu Li-Jia Qu | 2011 | Journal of Integrative Plant Biology2011，53，6： | 23 |
| 2 | Tiny YOLO Optimization Oriented Bus Passenger Object Detection显示文摘The real-time collection of bus passenger object detection is an essential part of developing a smart bus system.The difficulty of object detection mainly lies in the objective factors,such as:clothing,hair style and accessories,light,etc.Traditional object detection met hods with the arti ficial feature ext rac tion suffers from insufficien t st reng th in expression,generalization,and recogni tion rate.The objec t detection met hod based on deep learning mainly uses the convol utio nal neural net work in deep learning to learn features from a large set of data.The learned features can describe the rich infbrmation inherent in the data,and improve the expression ability of the features as well as the recognition accuracy.Due to too many parameters of the Convolutional neural network(CNN)model,the amount of calculation is too large to be opera ted on the vehicle terminal.To reduce calculation burden and improve the operation speed,we employs the depthwise separable convolution method to optimize the convolutional layer of tiny YOLO net work model.It decomposes a complete convolution operation into depthwise convolution and pointwise convolution,thus reducing the parameter amount of the CNN and improving the operation speed.The experiment results reveal that the speed of bus passenger object detection detected by our improved model is 4 times faster than the previous one but with the nearly same detection accuracy. | ZHANG Shuo WU Yanxia MEN Chaoguang LI Xiaosong | 2020 | Chinese Journal of Electronics2020，29，1： | 15 |
| 3 | Evaluating and Improving Wind Forecasts over South China: The Role of Orographic Parameterization in the GRAPES Model显示文摘Unresolved small-scale orographic(SSO) drags are parameterized in a regional model based on the Global/Regional Assimilation and Prediction System for the Tropical Mesoscale Model(GRAPES TMM). The SSO drags are represented by adding a sink term in the momentum equations. The maximum height of the mountain within the grid box is adopted in the SSO parameterization(SSOP) scheme as compensation for the drag. The effects of the unresolved topography are parameterized as the feedbacks to the momentum tendencies on the first model level in planetary boundary layer(PBL)parameterization. The SSOP scheme has been implemented and coupled with the PBL parameterization scheme within the model physics package. A monthly simulation is designed to examine the performance of the SSOP scheme over the complex terrain areas located in the southwest of Guangdong. The verification results show that the surface wind speed bias has been much alleviated by adopting the SSOP scheme, in addition to reduction of the wind bias in the lower troposphere. The target verification over Xinyi shows that the simulations with the SSOP scheme provide improved wind estimation over the complex regions in the southwest of Guangdong. | Shuixin ZHONG Zitong CHEN Daosheng XU Yanxia ZHANG | 2018 | Advances in Atmospheric Sciences2018，35，6： | 12 |
| 4 | A human circulating immune cell landscape in aging and COVID-19显示文摘Age-associated changes in immune cells have been linked to an increased risk for infection.However,a global and detailed characterization of the changes that human circulating immune cells undergo with age is lacking.Here,we combined scRNA-seq,mass cytometry and sCATAC-seq to compare immune cell types in peripheral blood collected from young and old subjects and patients with COVID-19.We found that the immune cell landscape was reprogrammed with age and was characterized by T cell polarization from naive and memory cells to effector,cytotoxic,exhausted and reg-ulatory cells,along with increased late natural killer cells,age-associated B cells,inflammatory monocytes and age-associated dendritic cells.In addition,the expression of genes,which were implicated in coron-avirus susceptibility,was upregulated in a cell subtype-specific manner with age.Notably,COVID-19 promoted age-induced immune cell polarization and gene expression related to inflammation and cellular senes-cence.Therefore,these findings suggest that a dysreg-ulated immune system and increased gene expression associated with SARS-CoV-2 susceptibility may at least partially account for COVID-19 vulnerability in the elderly. | Yingfeng Zheng Xiuxing Liu Wenqing Le Lihui Xie He Li Wen Wen Si Wang Shuai Ma Zhaohao Huang Jinguo Ye Wen Shi Yanxia Ye Zunpeng Liu Moshi Song Weiqi Zhang Jing-Dong J.Han Juan Carlos lzpisua Belmonte Chuanle Xiao Jing Qu Hongyang Wang Guang-Hui Liu Wenru Su | 2020 | Protein & Cell2020，11，10： | 10 |
| 5 | Vitamin C alleviates aging defects in a stem cell model for Werner syndrome显示文摘沃纳症候群(WS ) 是主要影响从中层导出的纸巾的早衰混乱。我们最近用 WRN 缺乏的人的间充质的干细胞(MSC ) 开发了一个新奇人的 WS 模型。这个模型概括 WS 的许多 phenotypic 特征。基于很多化学药品的一幅屏幕,这里,我们发现维生素 C 在出现在WRN缺乏的 MSC 的早衰为许多特征施加很有效的营救包括细胞生长拘捕,增加了反应的氧种层次, telomere 摩擦,煽动性的因素的过多的分泌物,象原子薄板和 heterochromatin 的组织的破坏一样。而且,维生素 C 在一个老鼠模型在 vivo 恢复 MSC 的生存能力。定序分析的 RNA 显示维生素 C 改变涉及染色质冷凝作用,房间周期规定, DNA 复制,和 DNA 损坏的一系列基因的表示在 WRN 缺乏的 MSC 的修理小径。我们的结果为 WS MSC 作为一个恢复因素识别维生素 C,它保持作为 WS 的处理的一种新奇类型被使用的潜力。 | Ying Li Weizhou Zhang Liang Chang Yan Han Liang Sun Xiaojun Gong Hong Tang Zunpeng Liu Huichao Deng Yanxia Ye Yu Wang Jian Li Jie Qiao Jing Qu Weiqi Zhang Guang-Hui Liu | 2016 | Protein & Cell2016，7，7： | 10 |
| 6 | Developing superior alleles of yield genes in rice by artificial mutagenesis using the CRISPR/Cas9 system显示文摘Rice yield is an important and complex agronomic trait controlled by multiple genes.In recent decades,dozens of yield-associated genes in rice have been cloned,many of which can increase production in the form of loss or degeneration of function.However,mutations occurring randomly under natural conditions have provided very limited genetic resources for yield increases.In this study,potentially yield-increasing alleles of two genes closely associated with yield were edited artificially.The recently developed CRISPR/Cas9system was used to edit two yield genes:Grain number 1a(Gn1a)and DENSE AND ERECT PANICLE1(DEP1).Several mutants were identified by a target sequence analysis.Phenotypic analysis confirmed one mutant allele of Gn1a and three of DEP1 conferring yield superior to that conferred by other natural high-yield alleles.Our results demonstrate that favorable alleles of the Gnla and DEP1 genes,which are considered key factors in rice yield increases,could be developed by artificial mutagenesis using genome editing technology. | Liyu Huang Ru Zhang Guangfu Huang Yanxia Li Getachew Melaku Shilai Zhang Haitao Chen Yanjuan Zhao Jing Zhang Yesheng Zhang Fengyi Hu | 2018 | The Crop Journal2018，6，5： | 8 |
| 7 | Satellite-observed trends in the Arctic sea ice concentration for the period 1979–2016显示文摘Arctic sea ice cover has decreased dramatically over the last three decades. This study quanti?es the sea ice concentration(SIC) trends in the Arctic Ocean over the period of 1979–2016 and analyzes their spatial and temporal variations. During each month the SIC trends are negative over the Arctic Ocean, wherein the largest(smallest) rate of decline found in September(March) is-0.48%/a(-0.10%/a).The summer(-0.42%/a) and autumn(-0.31%/a) seasons show faster decrease rates than those of winter(-0.12%/a) and spring(-0.20%/a) seasons. Regional variability is large in the annual SIC trend. The largest SIC trends are observed for the Kara(-0.60%/a) and Barents Seas(-0.54%/a), followed by the Chukchi Sea(-0.48%/a), East Siberian Sea(-0.43%/a), Laptev Sea(-0.38%/a), and Beaufort Sea(-0.36%/a). The annual SIC trend for the whole Arctic Ocean is-0.26%/a over the same period. Furthermore, the in?uences and feedbacks between the SIC and three climate indexes and three climatic parameters, including the Arctic Oscillation(AO), North Atlantic Oscillation(NAO), Dipole anomaly(DA), sea surface temperature(SST), surface air temperature(SAT), and surface wind(SW), are investigated. Statistically, sea ice provides memory for the Arctic climate system so that changes in SIC driven by the climate indices(AO, NAO and DA) can be felt during the ensuing seasons. Positive SST trends can cause greater SIC reductions, which is observed in the Greenland and Barents Seas during the autumn and winter. In contrast, the removal of sea ice(i.e., loss of the insulating layer) likely contributes to a colder sea surface(i.e., decreased SST), as is observed in northern Barents Sea. Decreasing SIC trends can lead to an in-phase enhancement of SAT, while SAT variations seem to have a lagged in?uence on SIC trends. SW plays an important role in the modulating SIC trends in two ways: by transporting moist and warm air that melts sea ice in peripheral seas(typically evident inthe Barents Sea) and by exporting sea ice out of the Arctic Ocean via passages into the Greenland and Barents Seas, including the Fram Strait, the passage between Svalbard and Franz Josef Land(S-FJL),and the passage between Franz Josef Land and Severnaya Zemlya(FJL-SZ). | WANG Yunhe BI Haibo HUANG Haijun LIU Yanxia LIU Yilin LIANG Xi FU Min ZHANG Zehua | 2019 | Journal of Oceanology and Limnology2019，37，1： | 7 |
| 8 | Release of VEGF and BMP9 from injectable alginate based composite hydrogel for treatment of myocardial infarction显示文摘Myocardial infarction(MI)is one of cardiovascular diseases that pose a serious threat to human health.The pathophysiology of MI is complex and contains several sequential phases including blockage of a coronary artery,necrosis of myocardial cells,inflammation,and myocardial fibrosis.Aiming at the treatment of different stages of MI,in this work,an injectable alginate based composite hydrogel is developed to load vascular endothelial active factor(VEGF)and silk fibroin(SF)microspheres containing bone morphogenetic protein 9(BMP9)for releasing VEGF and BMP9 to realize their respective functions.The results of in vitro experiments indicate a rapid initial release of VEGF during the first few days and a relatively slow and sustained release of BMP9 for days,facilitating the formation of blood vessels in the early stage and inhibiting myocardial fibrosis in the long-term stage,respectively.Intramyocardial injection of such composite hydrogel into the infarct border zone of mice MI model via multiple points promotes angiogenesis and reduces the infarction size.Taken together,these results indicate that the dual-release of VEGF and BMP9 from the composite hydrogel results in a collaborative effect on the treatment of MI and improvement of heart function,showing a promising potential for cardiac clinical application. | Yong Wu Tianqi Chang Weiqian Chen Xiaoyu Wang Jingjing Li Yueqiu Chen You Yu Zhenya Shen Qian Yu Yanxia Zhang | 2021 | Bioactive Materials2021，6，2： | 7 |
| 9 | B cells biology in systemic lupus erythematosus—from bench to bedside显示文摘Systemic lupus erythematosus(SLE) is a debilitating autoimmune disease that can involve multi-organs. B cells play a central role in the immunopathogenesis via antibody-dependent and antibody-independent ways. Excessive autoantibodies production, hyperresponsiveness and prolonged survival of autoreactive B cells are characteristics of SLE. In this article, mechanisms of self-tolerance loss of B cells and promising B cell-targeting therapies in lupus are reviewed and discussed. | ZHAO LiDan YE YanXia ZHANG Xuan | 2015 | Science China(Life Sciences)2015，58，11： | 7 |
| 10 | Changes in Physicochemical,Cooking and Sensory Characteristics of Rice Shifted from Low-temperature Storage显示文摘The physicochemical, cooking, and sensory characteristics of stored rice were measured in order to investigate the quality changes in it after low-temperature storage. The quality of the stored rice was compared to that of the rice stored at ambient temperature(20℃, 30%–70% RH) at a 25 d interval during 200 d of storage. The rice was stored in a temperature controller at 4℃ for 3 months and later under a simulated condition similar to that of the main rice consumption areas in China(35℃, 80% RH and 30℃, 70% RH). The results showed that the fatty acid value, b value, and moisture content of rice stored at 35℃, 80% RH and 30℃, 70% RH had increased significantly, whereas its L value had decreased as compared to the rice stored at ambient temperature. Higher temperature storage caused a greater water uptake, whereas the dry mass in the residual cooking water notably reduced under the storage at 35℃ as compared to that at 20℃. Hardness increased and adhesiveness reduced under the storage at 35℃ as compared to that at 20℃. The shelf life of the stored rice which was shifted from a low temperature to three storage conditions used in this study was 75 d, 100 d and 150 d, respectively, in the main rice consumption areas of China. | AN Yanxia ZHOU Xianqing ZHANG Yurong | 2018 | Grain & Oil Science and Technology2018，1，1： | 6 |
| 11 | Investigation of carbon fiber composite stiffened skin with vacuum assisted resin infusion/prepreg co-curing process显示文摘Co-cured vacuum assisted resin infusion process(co-VARI process),which combined vacuum assisted resin infusion(VARI)with prepreg vacuum bag only process(VBO),was adopted to fabricate T-shaped stiffened skin with non-crimp fabric(NCF)stiffener and prepreg skin.During compaction stage of co-VARI process,prepreg resin impregnated fiber fabric under elevated temperature and vacuum pressure.This phenomenon was characterized by fluorescent micrographs with different holding temperature and time.Its influences on processing quality and mechanical performance for co-VARI stiffened skin with different filler materials at triangular region were further analyzed by optical micrographs and pull-off test,respectively.The results show that increasing holding temperature and prolonging holding time can promote prepreg resin impregnation in fiber fabric.Moderate prepreg resin impregnation is favorable to reduce resin rich region and increase fiber volume fraction at prepreg-fabric interface.Moreover,prepreg resin impregnation effect plays significant roles on pull-off performance for co-VARI stiffened skin with fabric filler but has negligible influences on specimens with prepreg filler.In addition,compared with stiffened skin with fabric filler,superior processing quality and pull-off performances are achieved for co-VARI stiffened skin with prepreg core filler.These results are helpful to optimize processing procedures and fabricate composite structure by coVARI process. | MA XuQiang GU YiZhuo LI Min LI YanXia ZHANG ZuoGuang | 2014 | Science China(Technological Sciences)2014，57，10： | 6 |
| 12 | A SVM-kNN method for quasar-star classification显示文摘We integrate k-Nearest Neighbors(kNN) into Support Vector Machine(SVM) and create a new method called SVM-kNN.SVM-kNN strengthens the generalization ability of SVM and apply kNN to correct some forecast errors of SVM and improve the forecast accuracy.In addition,it can give the prediction probability of any quasar candidate through counting the nearest neighbors of that candidate which is produced by kNN.Applying photometric data of stars and quasars with spectral classification from SDSS DR7 and considering limiting magnitude error is less than 0.1,SVM-kNN and SVM reach much higher performance that all the classification metrics of quasar selection are above 97.0%.Apparently,the performance of SVM-kNN has slighter improvement than that of SVM.Therefore SVM-kNN is such a competitive and promising approach that can be used to construct the targeting catalogue of quasar candidates for large sky surveys. | PENG NanBo ZHANG YanXia ZHAO YongHeng | 2013 | Science China(Physics,Mechanics & Astronomy)2013，56，6： | 6 |
| 13 | Robust YAG:Ce single crystal for ultra-high efficiency laser lighting显示文摘At present,solid-state laser-driven lighting fabricated with blue laser diode(LD)and phosphor has been greatly developed in high-power lighting applications.The major obstacle is the luminescence saturation threshold of the fluorescence conversion material(YAG:Ce).In this work,we successfully prepared a series of high-quality and robust YAG:Ce single crystals.With high quantum efficiency(QE=92%),regular crystal structure and high thermal conductivity,the single-crystal has excellent ability to deal with high radiation flux and thermal accumulation generated by high power.Matching the single-crystal with the laser,the luminous efficiency up to 274 lm/W and saturation threshold of 12 W/mm^(2) were obtained.In addition,the influence of Ce doping concentration and sample processing conditions on the indicators in laser-driven white lighting was systematically studied.The results are of great significance and are believed to promote the development of solid-state laser-driven lighting. | Yaqian Zhang Jianming Liu Yujie Zhang Haisheng Yang Yanxia Yu Qingyun He Xiaojuan Liang Yongfu Liu Weidong Xiang | 2022 | Journal of Rare Earths2022，40，5： | 5 |
| 14 | Fabrication of aluminum matrix composite reinforced with carbon nanotubes显示文摘1.0wt.% carbon nanotube (CNT) reinforced 2024Al matrix composite was fabricated by cold isostatic press and subsequent hot extrusion techniques. The mechanical properties of the composite were measured by a tensile test. Mean-while,the fracture surfaces were examined using field emission scanning electron microscopy. The experimental results show that CNTs are dispersed homogeneously in the composite and that the interfaces of the Al matrix and the CNT bond well. Although the tensile strength and the Young’s modulus of the composite are enhanced markedly,the elongation does not decrease when compared with the matrix material fabricated under the same process. The reasons for the increments may be the extraordinary mechanical properties of CNTs,and the bridging and pulling-out role of CNTs in the Al matrix composite. | DENG Chunfeng ZHANG Xuexi MA Yanxia WANG Dezun | 2007 | Rare Metals2007，26，5： | 5 |
| 15 | Arctic sea ice volume export through the Fram Strait from combined satellite and model data:1979–2012显示文摘By combing satellite-derived ice motion and concentration with ice thickness fields from a popular model PIOMAS we obtain the estimates of ice volume flux passing the Fram Strait over the 1979–2012 period. Since current satellite and field observations for sea ice thickness are limited in time and space, the use of PIOMAS is expected to fill the gap by providing temporally continued ice thickness fields. Calculated monthly volume flux exhibits a prominent annual cycle with the peak record in March(roughly 145 km3/month) and the trough in August(10 km^3/month). Annual ice volume flux(1 132 km^3) is primarily attributable to winter(October through May) outflow(approximately 92%). Uncertainty in annual ice volume export is estimated to be 55 km^3(or 5.7%). Our results also verified the extremely large volume flux appearing between late 1980 s and mid-1990 s. Nevertheless, no clear trend was found in our volume flux results. Ice motion is the primary factor in the determination of behavior of volume flux. Ice thickness presented a general decline trend may partly enhance or weaken the volume flux trend. Ice concentration exerted the least influences on modulating trends and variability in volume flux. Moreover, the linkage between winter ice volume flux and three established Arctic atmospheric schemes were examined. Compared to NAO, the DA and EOF3 mechanism explains a larger part of variations of ice volume flux across the strait. | ZHANG Zehua BI Haibo SUN Ke HUANG Haijun LIU Yanxia YAN Liwen | 2017 | Acta Oceanologica Sinica2017，36，1： | 4 |
| 16 | Construction of cDNA library from iron-deficiency induced maize roots and screening and identification of iron-stress gene Fdr3显示文摘To isolate Fe-deficient related (Fdr) genes, an expression cDNA library of 4.5×105 pfu/μg has been constructed from maize roots in iron-stress. 6 clones have been screened from the cDNA library by differential hybridization screening. It is proved that an Fdr3 cDNA clone expressed stronger under iron-deficient condition than under iron-sufficient one by Northern blot and Western blot. | YIN Liping QI Xiaoting LIU Xianglin HUO Chunyan BAI Yanxia QIU Zesheng ZHANG Fusuo | 2000 | Chinese Science Bulletin2000，45，12： | 4 |
| 17 | Comparative evaluation of the impact of GRAPES and MM5 meteorology on CMAQ prediction over Pearl River Delta, China显示文摘The community multiscale air quality (CMAQ) model was used to forecast air quality over the Pearl River Delta region from December 2013 to January 2014.The pollution forecasting performance of CMAQ coupled with two different meteorological models,i.e.,the global/regional assimilation and prediction system (GRAPES) and the fifth-generation mesoscale model (MM5),was assessed by comparison with observational data.The effects of meteorological factors and physicochemical processes on the forecast results were discussed through process analysis.The results showed that both models exhibited good performance but that of GRAPES-CMAQ was better.GRAPES was superior in predicting the overall variation tendencies of meteorological fields,but it showed large deviations in atmospheric pressure and wind speed.This contributed to the higher correlation coefficients of the pollutants with GRAPES-CMAQ but with greater deviations.The underestimations of nitrate and ammonium salt contributed to the underestimations of both particulate matter and extinction coefficients.Source emissions made the only positive contributions to surface layer SO2,CO,and NO.It was found that O3 originated primarily from horizontal and vertical transport and that its consumption was predominantly via chemical processes.Conversely,NO2 was found derived primarily from chemical production. | Tao Deng Yiang Chen Qilin Wan Yanxia Zhang Xuejiao Deng Yanyan Huang Guangfeng Dai Fei Li | 2018 | Particuology2018，16，5： | 4 |
| 18 | A model for nanogrinding based on direct evidence of ground chips of silicon wafers显示文摘Nanometer chips were directly fabricated using face nanogrinding carried out by ultrafine diamond grits at room temperature. Direct evidence for ground nanometer chips is cuboid, and the average ratio of width to thickness is 1.49. Chips of 9.0 nm in thickness, 13.3 nm in width, and 16.0 in diagonal were achieved and confirmed using transmission electron microscopy. Based on the nanometer chips observed, a model was proposed according to the mass conservation and fundamental mechanism of face grinding. The surface roughness and thickness of damaged layers measured experimentally are in good agreement with the prediction of the developed model. The feed rate significantly affects the surface roughness and thickness of damaged layers, when keeping the wheel and table speeds constant, respectively. | ZHANG ZhenYu HUO YanXia GUO DongMing | 2013 | Science China(Technological Sciences)2013，56，9： | 4 |
| 19 | Application of regional citrate anticoagulation in patients at high risk of bleeding during intermittent hemodialysis:a prospective multicenter randomized controlled trial显示文摘Objective:Safe and effective anticoagulation is essential for hemodialysis patients who are at high risk of bleeding.The purpose of this trial is to evaluate the effectiveness and safety of two-stage regional citrate anticoagulation(RCA)combined with sequential anticoagulation and standard calcium-containing dialysate in intermittent hemodialysis(IHD)treatment.Methods:Patients at high risk of bleeding who underwent IHD from September 2019 to May 2021 were prospectively enrolled in 13 blood purification centers of nephrology departments,and were randomly divided into RCA group and saline flushing group.In the RCA group,0.04 g/mL sodium citrate was infused from the start of the dialysis line during blood draining and at the venous expansion chamber.The sodium citrate was stopped after 3 h of dialysis,which was changed to sequential dialysis without anticoagulant.The hazard ratios for coagulation were according to baseline.Results:A total of 159 patients and 208 sessions were enrolled,including RCA group(80 patients,110 sessions)and saline flushing group(79 patients,98 sessions).The incidence of severe coagulation events of extracorporeal circulation in the RCA group was significantly lower than that in the saline flushing group(3.64%vs.20.41%,P<0.001).The survival time of the filter pipeline in the RCA group was significantly longer than that in the saline flushing group((238.34±9.33)min vs.(221.73±34.10)min,P<0.001).The urea clearance index(Kt/V)in the RCA group was similar to that in the saline flushing group with no statistically significant difference(1.12±0.34 vs.1.08±0.34,P=0.41).Conclusions:Compared with saline flushing,the two-stage RCA combined with a sequential anticoagulation strategy significantly reduced extracorporeal circulation clotting events and prolonged the dialysis time without serious adverse events. | Xiaoyan TANG Dezheng CHEN Ling ZHANG Ping FU Yanxia CHEN Zhou XIAO Xiangcheng XIAO Weisheng PENG Li CHENG Yanmin ZHANG Hongbo LI Kehui LI Bizhen GOU Xin WU Qian YU Lijun JIAN Zaizhi ZHU Yu WEN Cheng LIU Hen XUE Hongyu ZHANG Xin HE Bin YAN Liping ZHONG Bin HUANG Mingying MAO | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，11： | 4 |
| 20 | Impact of diabetes on promoting the growth of breast cancer显示文摘Background:Type Ⅱ diabetes mellitus(DM2)is a significant risk factor for cancers,including breast cancer.However,a proper diabetic breast cancer mouse model is notwell-established for treatment strategy design.Additionally,the precise diabetic signaling pathways that regulate cancer growth remain unresolved.In the present study,we established a suitable mouse model and demonstrated the pathogenic role of diabetes on breast cancer progression.Methods:We successfully generated a transgenic mouse model of human epidermal growth factor receptor 2 positive(Her2^(+) or ERBB2)breast cancer with DM2 by crossing leptin receptor mutant(Lepr^(db/+))mice with (MMTV-ErbB2/neu)mice.Themousemodelswere administrated with antidiabetic drugs to assess the impacts of controlling DM2 in affecting tumor growth.Magnetic resonance spectroscopic imaging was employed to analyze the tumor metabolism.Results:Treatment with metformin/rosiglitazone in MMTV-ErbB2/Lepr^(db/db) mousemodel reduced serum insulin levels,prolonged overall survival,decreased cumulative tumor incidence,and inhibited tumor progression.Anti-insulin resistance medications also inhibited glycolytic metabolism in tumors in vivo as indicated by the reduced metabolic flux of hyperpolarized ^(13)C pyruvate-to-lactate reaction.The tumor cells from MMTV-ErbB2/Lepr^(db/db) transgenic mice treated with metformin had reprogrammed metabolism by reducing levels of both oxygen consumption and lactate production.Metformin decreased the expression of Myc and pyruvate kinase isozyme 2(PKM2),leading to metabolism reprogramming.Moreover,metformin attenuated the mTOR/AKT signaling pathway and altered adipokine profiles.Conclusions:MMTV-ErbB2/Lepr^(db/db) mouse model was able to recapitulate diabetic HER2^(+) human breast cancer.Additionally,our results defined the signaling pathways deregulated in HER2^(+) breast cancer under diabetic condition,which can be intervened by anti-insulin resistance therapy. | Ping-Chieh Chou Hyun Ho Choi Yizhi Huang Enrique Fuentes-Mattei Guermarie Velazquez-Torres Fanmao Zhang Liem Phan Jaehyuk Lee Yanxia Shi James A.Bankson Yun Wu Huamin Wang Ruiying Zhao Sai-Ching Jim Yeung Mong-Hong Lee | 2021 | Cancer Communications2021，41，5： | 4 |
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