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| 1 | Cloning of a gene-edited macaque monkey by somatic cell nuclear transfer显示文摘Cloning of macaque monkeys by somatic cell nucleus transfer(SCNT) allows the generation of monkeys with uniform genetic backgrounds that are useful for the development of non-human primate models of human diseases. Here, we report the feasibility of this approach by SCNT of fibroblasts from a macaque monkey(Macaca fascicularis), in which a core circadian transcription factor BMAL1 was knocked out by clustered regularly interspaced short palindromic repeat/Cas9 gene editing(see accompanying paper).Out of 325 SCNT embryos transferred into 65 surrogate monkeys, we cloned five macaque monkeys with BMAL1 mutations in both alleles without mosaicism, with nuclear genes identical to that of the fibroblast donor monkey. Further peripheral blood m RNA analysis confirmed the complete absence of the wild-type BMAL1 transcript. This study demonstrates that the SCNT approach could be used to generate cloned monkeys from fibroblasts of a young adult monkeys and paves the way for the development of macaque monkey disease models with uniform genetic backgrounds. | Zhen Liu Yijun Cai Zhaodi Liao Yuting Xu Yan Wang Zhanyang Wang Xiaoyu Jiang Yuzhuo Li Yong Lu Yanhong Nie Xiaotong Zhang Chunyang Li Xinyan Bian Mu-ming Poo Hung-Chun Chang Qiang Sun | 2019 | National Science Review2019，6，1： | 14 |
| 2 | Numerical simulation and experimental investigation of natural convection heat transfer of molten salt around fine wire显示文摘In order to get the natural convection heat transfer mechanism of molten salt, the experimental investigation of natural convective heat transfer of LiNO3was studied after it was simulated by numerical calculation. Experiment was carried out on the nat-ural convection heat transfer of air and water around the fine wire using the method of Joule heating. The results showed that the natural convection heat transfer of air and water around the fine wire agreed well with Fand's correlation. Based on the aforementioned experiment, the natural convection heat transfer of molten salt LiNO3was studied by experiment and the same results were got. Therefore, the natural convection heat transfer of molten salt can be calculated by Fand's correlation, which takes into consideration the effect of viscosity dissipation. | LU YuanWei LI XiaoLi LI Qiang WU YuTing MA ChongFang | 2013 | Science China(Technological Sciences)2013，56，7： | 13 |
| 3 | Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells显示文摘RNase III DROSHA is upregulated in multiple cancers and contributes to tumor progression by hitherto unclear mechanisms.Here,we demonstrate that DROSHA interacts withβ-Catenin to transactivate STC1 in an RNA cleavage-independent manner,contributing to breast cancer stem-like cell(BCSC)properties.DROSHA mRNA stability is enhanced by N6-methyladenosine(m^(6)A)modification which is activated by AURKA in BCSCs.AURKA stabilizes METTL14 by inhibiting its ubiquitylation and degradation to promote DROSHA mRNA methylation.Moreover,binding of AURKA to DROSHA transcript further strengthens the binding of the m^(6)A reader IGF2BP2 to stabilize m^(6)A-modified DROSHA.In addition,wild-type DROSHA,but not an m^(6)A methylation-deficient mutant,enhances BCSC stemness maintenance,while inhibition of DROSHA m^(6)A modification attenuates BCSC traits.Our study unveils the AURKA-induced oncogenic m^(6)A modification as a key regulator of DROSHA in breast cancer and identifies a novel DROSHA transcriptional function in promoting the BCSC phenotype. | Fei Peng Jie Xu Bai Cui Qilan Liang Sai Zeng Bin He Hong Zou Manman Li Huan Zhao Yuting Meng Jin Chen Bing Liu Shasha Lv Peng Chu Fan An Zifeng Wang Junxiu Huang Yajing Zhan Yuwei Liao Jinxin Lu Lingzhi Xu Jin Zhang Zhaolin Su Zhiguang Li Fangjun Wang Eric W-FLam Quentin Liu | 2021 | Cell Research2021，31，3： | 9 |
| 4 | Targeting and exploitation of tumor-associated neutrophils to enhance immunotherapy and drug delivery for cancer treatment显示文摘Neutrophils,the most abundant leukocytes in human blood,are essential fighter immune cells against microbial infection.Based on the finding that neutrophils can either restrict or promote cancer progression,tumor-associated neutrophils(TAN)are classified into anti-tumor N1 and pro-tumor N2 subsets.One of the major mechanisms underlying the tumor-promoting function of N2-TANs is suppression of adaptive immune cells,in particular,cytotoxic T lymphocytes.Currently,no established methodologies are available that can unequivocally distinguish immunosuppressive TANs and granulocytic/polymorphonuclear myeloid-derived suppressor cells(G/PMN-MDSC).In view of the critical role of PMN-MDSCs in immune evasion and resistance to cancer immunotherapy,as established from data obtained with diverse cancer models,therapeutic strategies targeting these cells have been actively developed to enhance the efficacy of immunotherapy.Here,we have reviewed the available literature on strategies targeting PMN-MDSCs and summarized the findings into four categories:(1)depletion of existing PMN-MDSCs,(2)blockade of the development of PMNMDSCs,(3)blockade of PMN-MDSC recruitment,(4)inhibition of immunosuppressive function.Owing to their high mobility to inflamed organs and ability to trespass the blood-brain barrier,neutrophils are outstanding candidate carriers in nanoparticle-based therapies.Another attractive application of neutrophils in cancer therapy is the use of neutrophil membrane-derived nanovesicles as a surrogate of extracellular vesicles for more efficient and scalable drug delivery.In the second part of the review,we have highlighted recent advances in the field of neutrophil-based cancer drug delivery.Overall,we believe that neutrophil-based therapeutics are a rapidly growing area of cancer therapy with significant potential benefits. | Yuting Zhang Guoqiang Liu Miaomiao Sun Xin Lu | 2020 | Cancer Biology & Medicine2020，17，1： | 9 |
| 5 | Natural convection heat transfer of molten salt in a single energy storage tank显示文摘在在一辆柱体坦克的熔融的盐的热转移经由模拟和实验被学习在一辆单个精力存储坦克获得它的自然传送对流热转移。模拟和在一辆柱体坦克的水的自然传送对流热转移与 Garons 关联适合很好的试验性的结果表演。然而,当因为它的高粘性和低热电导率, Garons 关联被用来预言熔融的盐的自然传送对流热转移时,重要偏差发生。然而,熔融的盐的自然传送对流热转移的模仿的数据试验性的结果与那些适合很好。因此,考虑可变物理性质的效果的关联在这研究被建议预言熔融的盐的自然传送对流热转移。从建议关联的现在的数据的偏差是不到 d 控制模拟。在 summe 枼 ? 枼期间?? | LU YuanWei YU Qiang DU WenBin WU YuTing MA ChongFang | 2016 | Science China(Technological Sciences)2016，59，8： | 7 |
| 6 | A comprehensive update:gastrointestinal microflora,gastric cancer and gastric premalignant condition,and intervention by traditional Chinese medicine显示文摘With the recent upsurge of studies in the field of microbiology,we have learned more about the complexity of the gastrointestinal microecosystem.More than 30 genera and 1000 species of gastrointestinal microflora have been found.The structure of the normal microflora is relatively stable,and is in an interdependent and restricted dynamic equilibrium with the body.In recent years,studies have shown that there is a potential relationship between gastrointestinal microflora imbalance and gastric cancer(GC)and precancerous lesions.So,restoring the balance of gastrointestinal microflora is of great significance.Moreover,intervention in gastric premalignant condition(GPC),also known as precancerous lesion of gastric cancer(PLGC),has been the focus of current clinical studies.The holistic view of traditional Chinese medicine(TCM)is consistent with the microecology concept,and oral TCM can play a two-way regulatory role directly with the microflora in the digestive tract,restoring the homeostasis of gastrointestinal microflora to prevent canceration.However,large gaps in knowledge remain to be addressed.This review aims to provide new ideas and a reference for clinical practice. | Yuting LU Huayi LIU Kuo YANG Yijia MAO Lingkai MENG Liu YANG Guangze OUYANG Wenjie LIU | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，1： | 6 |
| 7 | Genome-and transcriptome-wide association studies provide insights into the genetic basis of natural variation of seed oil content in Brassica napus显示文摘Seed oil content(SOC)is a highly important and complex trait in oil crops.Here,we decipher the genetic basis of natural variation in SOC of Brassica napus by genome-and transcriptome-wide association studies using 505 inbred lines.We mapped reliable quantitative trait loci(QTLs)that control SOC in eight environments,evaluated the effect of each QTL on SOC,and analyzed selection in QTL regions during breeding.Six-hundred and ninety-two genes and four gene modules significantly associated with SOC were identified by analyzing population transcriptomes from seeds.A gene prioritization framework,POCKET(prioritizing the candidate genes by incorporating information on knowledge-based gene sets,effects of variants,genome-wide association studies,and transcriptome-wide association studies),was implemented to determine the causal genes in the QTL regions based on multi-omic datasets.A pair of homologous genes,BnPMT6s,in two QTLs were identified and experimentally demonstrated to negatively regulate SOC.This study provides rich genetic resources for improving SOC and valuable insights toward understanding the complex machinery that directs oil accumulation in the seeds of B.napus and other oil crops. | Shan Tang Hu Zhao Shaoping Lu Liangqian Yu Guofang Zhang Yuting Zhang Qing-Yong Yang Yongming Zhou Xuemin Wang Wei Ma Weibo Xie Liang Guo | 2021 | Molecular Plant2021，14，3： | 6 |
| 8 | Performance Analysis of WLAN Medium Access Control Protocols in Simulcast Radio-Over-Fiber-Based Distributed Antenna Systems显示文摘The performance of three wireless local-area network(WLAN) media access control(MAC) protocols is investigated and compared in the context of simulcast radioover-fiber-based distributed antenna systems(RoF-DASs) where multiple remote antenna units(RAUs) are connected to one access point(AP) with different-length fiber links.The three WLAN MAC protocols under investigation are distributed coordination function(DCF) in basic access mode,DCF in request/clear to send(RTS/CTS) exchange mode,and point coordination function(PCF).In the analysis,the inter-RAU hidden nodes problems and fiber-length difference effect are both taken into account.Results show that adaptive PCF mechanism has better throughput performances than the other two DCF modes,especially when the inserted fiber length is short. | LI Jianqiang FAN Yuting CHEN Hao LU Xun XU Kun | 2014 | China Communications2014，11，5： | 5 |
| 9 | Mitochondrial replacement in macaque monkey offspring by first polar body transfer显示文摘Dear Editor,Mitochondrial DNA(mtDNA)mutations are materally inherited to the offspring and mitochondrial replacement therapy(MRT)provides.a promising approach for preventing mtDNA-related 1,2 diseases.First polar body transfer(PB1T)has been used for mitochondrial replacement to generate mouse offspring and normal human embryos that yield embryonic stem cells.However,generation of primate offspring using PB1T has not been reported.Here,we show that PB1T-based mitochondrial replacement could generate healthy macaque monkey(Macaca fascicularis),a useful non-human primate model for evaluating methods that may improve the efficiency and safety of PB1T for MRT. | Zhanyang Wang Yuzhuo Li Xianfa Yang Yan Wang Yanhong Nie Yuting Xu Xiaotong Zhang Yong Lu Tikui Zhang Qiming Liu Naihe Jing Zhen Liu Qiang Sun | 2021 | Cell Research2021，31，2： | 4 |
| 10 | Generation of obese rat model by transcription activator-like effector nucleases targeting the leptin receptor gene显示文摘The laboratory rat is a valuable mammalian model organism for basic research and drug discovery. Here we demonstrate an efficient methodology by applying transcription activator-like effector nucleases(TALENs) technology to generate Leptin receptor(Lepr) knockout rats on the Sprague Dawley(SD) genetic background. Through direct injection of in vitro transcribed m RNA of TALEN pairs into SD rat zygotes, somatic mutations were induced in two of three resulting pups. One of the founders carrying bi-allelic mutation exhibited early onset of obesity and infertility. The other founder carried a chimeric mutation which was efficiently transmitted to the progenies. Through phenotyping of the resulting three lines of rats bearing distinct mutations in the Lepr locus, we found that the strains with a frame-shifted or premature stop codon mutation led to obesity and metabolic disorders. However, no obvious defect was observed in a strain with an in-frame 57 bp deletion in the extracellular domain of Lepr. This suggests the deleted amino acids do not significantly affect Lepr structure and function. This is the first report of generating the Lepr mutant obese rat model in SD strain through a reverse genetic approach. This suggests that TALEN is an efficient and powerful gene editing technology for the generation of disease models. | Yuting Chen Wenqing Lu Na Gao Yi Long Yanjiao Shao Meizhen Liu Huaqing Chen Shixin Ye Xueyun Ma Mingyao Liu Dali Li | 2017 | Science China(Life Sciences)2017，60，2： | 4 |
| 11 | Flexible Operation Mode of Coal-fired Power Unit Coupling with Heat Storage of Extracted Reheat Steam显示文摘In order to provide more grid space for the renewable energy power,the traditional coal-fired power unit should be operated flexibility,especially achieved the deep peak shaving capacity.In this paper,a new scheme using the reheat steam extraction is proposed to further reduce the load far below 50%rated power.Two flexible operation modes of increasing power output mode and reducing fuel mode are proposed in heat discharging process.A 600 MW coal-fired power unit with 50%rated power is chosen as the research model.The results show that the power output is decreased from 300.03 MW to 210.07 MW when the extracted reheat steam flow rate is 270.70 t·h^(-1),which increases the deep peak shaving capacity by 15%rated power.The deep peak shaving time and the thermal efficiency are 7.63 h·d^(-1)and 36.91%respectively for the increasing power output mode,and they are 7.24 h·d^(-1)and 36.58%respectively for the reducing fuel mode.The increasing power output mode has the advantages of higher deep peak shaving time and the thermal efficiency,which is recommended as the preferred scheme for the flexible operation of the coal-fired power unit. | WEI Haijiao LU Yuanwei YANG Yanchun ZHANG Cancan WU Yuting LI Weidong ZHAO Dongming | 2022 | Journal of Thermal Science2022，31，2： | 3 |
| 12 | GCN2 deficiency protects mice from denervation-induced skeletal muscle atrophy via inhibiting FoxO3a nuclear translocation显示文摘 | Yuting Guo Huiwen Wang Yinglong Tang Yue Wang Mengqi Zhang Zhiguang Yang Eric Nyirimigabo Bin Wei Zhongbing Lu Guangju Ji | 2018 | Protein & Cell2018，9，11： | 3 |
| 13 | A selected population study reveals the biochemical mechanism of intramuscular fat deposition in chicken meat显示文摘Background:Increasing intramuscular fat(IMF)is an important strategy to improve meat quality,but the regulation mechanism of IMF deposition needs to be systematically clarified.Results:A total of 520 chickens from a selected line with improved IMF content and a control line were used to investigate the biochemical mechanism of IMF deposition in chickens.The results showed that the increased IMF would improve the flavor and tenderness quality of chicken meat.IMF content was mainly determined both by measuring triglyceride(TG)and phospholipid(PLIP)in muscle tissue,but only TG content was found to be decisive for IMF deposition.Furthermore,the increase in major fatty acid(FA)components in IMF is mainly derived from TGs(including C16:0,C16:1,C18:1n9c,and C18:2n6c,etc.),and the inhibition of certain very-long-chain FAs would help to IMF/TG deposition.Conclusions:Our study elucidated the underlying biochemical mechanism of IMF deposition in chicken:Prevalent accumulation of long-chain FAs and inhibitions of medium-chain FAs and very long chain FA would jointly result in the increase of TGs with the FA biosynthesis and cellular uptake ways.Our findings will guide the production of high-quality chicken meat. | Huanxian Cui Lu Liu Xiaojing Liu Yongli Wang Na Luo Xiaodong Tan Yuting Zhu Ranran Liu Guiping Zhao Jie Wen | 2022 | Journal of Animal Science and Biotechnology2022，13，5： | 2 |
| 14 | Design,synthesis,and biological evaluation of Bcr-Abl PROTACs to overcome T315I mutation显示文摘Bcr-Abl threonine 315 to isoleucine 315(T315 I)gatekeeper mutation induced drug resistance remains an unmet clinical challenge for the treatment of chronic myeloid leukemia(CML).Chemical degradation of Bcr-AblT315 Iprotein has become a potential strategy to overcome drug resistance.Herein,we first described the design,synthesis,and evaluation of a new class of selective Bcr-AblT315 I proteolysis-targeting chimeric(PROTAC)degraders based on GZD824(reported as Bcr-AblT315 Iinhibitor by our group).One of the degrader 7 o with 6-member carbon chain linkage with pomalidomide exhibits the most potent degradation efficacy with DR of 69.89%and 94.23%at 100 and 300 nmol/L,respectively,and has an IC50 value of 26.8±9.7 nmol/L against Ba/F3T315 Icells.Further,7 o also displays substantial tumor regression against Ba/F3-Bcr-AblT315 Ixenograft model in vivo. | Liang Jiang Yuting Wang Qian Li Zhengchao Tu Sihua Zhu Sanfang Tu Zhang Zhang Ke Ding Xiaoyun Lu | 2021 | Acta Pharmaceutica Sinica B2021，11，5： | 2 |
| 15 | Surface sulfur vacancies enhanced electron transfer over Co-ZnS quantum dots for efficient degradation of plasticizer micropollutants by peroxymonosulfate activation显示文摘Peroxymonosulfate(PMS)activation in heterogeneous processes is a promising water treatment technology.Nevertheless,the high energy consumption and low efficiency during the reaction are ineluctable,due to electron cycling rate limitation.Herein,a new strategy is proposed based on a quantum dots(QDs)/PMS system.Co-ZnS QDs are synthesized by a water phase coprecipitation method.The inequivalent lattice-doping of Co for Zn leads to the generation of surface sulfur vacancies(SVs),which modulates the surface of the catalyst to form an electronic nonequilibrium surface.Astonishingly,the plasticizer micropollutants can be completely degraded within only tens of seconds in the Co-Zn S QDs/PMS system due to this type of surface modulation.The interfacial reaction mechanism is revealed that pollutants tend to be adsorbed on the cobalt metal sites as the electron donors,where the internal electrons of pollutants are captured by the metal species and transferred to the surface SVs.Meanwhile,PMS adsorbed on the SVs is reduced to radicals by capturing electrons,achieving effective electron recovery.Dissolved oxygen(DO)molecules are also easily attracted to catalyst defects and are reduced to O_(2)^(·-),further promoting the degradation of pollutants. | Yuting Gu Tingting Gao Fagen Zhang Chao Lu Wenrui Cao Ziwei Fu Chun Hu Lai Lyu | 2022 | Chinese Chemical Letters2022，33，8： | 2 |
| 16 | Therapeutic potential of traditional Chinese medicine for vascular endothelial growth factor显示文摘Vascular endothelial growth factor(VEGF)is the main regulator of physiological angiogenesis during embryonic development,bone growth,and reproductive function,and it also participates in a series of pathological changes.Traditional Chinese medicine(TCM),with a history of more than 2000 years,has been widely used in clinical practice,while the exploration of its mechanisms has only begun.This review summarizes the research of recent years on the influence of TCM on VEGF.It is found that many Chinese medicines and recipes have a regulatory effect on VEGF,indicating that Chinese medicine has broad prospects as a complementary and alternative therapy,providing new treatment ideas for clinical applications and the theoretical basis for research on the mechanisms of TCM. | Yijia MAO Lingkai MENG Huayi LIU Yuting LU Kuo YANG Guangze OUYANG Yanran BAN Shuang CHEN | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，5： | 1 |
| 17 | SERS study on the synergistic effects of electric field enhancement and charge transfer in an Ag2S quantum dots/plasmonic bowtie nanoantenna composite system显示文摘Localized surface plasmon resonance(LSPR)of nanostructures and the interfacial charge transfer(CT)of semiconductor materials play essential roles in the study of optical and photoelectronic properties.In this paper,a composite substrate of Ag2S quantum dots(QDs)coated plasmonic Au bowtie nanoantenna(BNA)arrays with a metalinsulator-metal(MIM)configuration was built to study the synergistic effect of LSPR and interfacial CT using surface-enhanced Raman scattering(SERS)in the near-infrared(NIR)region.The Au BNA array structure with a large enhancement of the localized electric field(E-field)strongly enhanced the Raman signal of adsorbed p-aminothiophenol(PATP)probe molecules.Meanwhile,the broad enhanced spectral region was achieved owing to the coupling of LSPR The as-prepared Au BNA array structure facilitated enhancements of the excitation as well as the emission of Raman signal simultaneously,which was established by finite-difference time-domain simulation.Moreover,Ag2S semiconductor QDs were introduced into the BNA/PATP system to further enhance Raman signals,which benefited from the interfacial CT resonance in the BNA/Ag2S-QDs/PATP system.As a result,the Raman signals of PATP in the BNA/Ag2S-QDs/PATP system were strongly enhanced under 785 nm laser excitation due to the synergistic effect of E-field enhancement and interfacial CT.Furthermore,the SERS polarization dependence effeas of the BNA/Ag2S-QDs/PATP system were also investigated.The SERS spectra indicated that the polarization dependence of the substrate increased with decreasing polarization angles(θpola)of excitation from p-polarized(θpola=90°)excitation to s-polarized(θpola=0°)excitation.This study provides a strategy using the synergistic effect of interfacial CT and E-field enhancement for SERS applications and provides a guidance for the development of SERS study on semiconductor QD-based plasmonic substrates,and can be farther extended to other material-nanostructure systems for various optoelectronic and sensing applications. | BIN WANG CHEN ZHAO HUANYU LU TINGTING ZOU SUBHASH C.SINGH ZHI YU CHAONAN YAO XIN ZHENG JUN XING YUTING ZOU CUNZHU TONG WEILI YU BO ZHAO CHUNLEI GUO | 2020 | Photonics Research2020，8，4： | 1 |
| 18 | Airborne quinones induce cytotoxicity and DNA damage in human lung epithelial A549 cells: The role of reactive oxygen species显示文摘 | Yu Shang Ling Zhang Yuting Jiang Yi Li Ping Lu | 2014 | Chemosphere2014，，： | 1 |
| 19 | Cinnamon and nisin in alginate–calcium coating maintain quality of fresh northern snakehead fish fillets显示文摘 | Fei Lu Yuting Ding Xingqian Ye Donghong Liu | 2010 | LWT - Food Science and Technology2010，，9： | 1 |
| 20 | Iteration convergence condition modeling for single spaceborne SAR image direct positioning显示文摘Single SAR image direct positioning is to determine the ground coordinate for each pixel in the SAR image assisted with a reference DEM.During this procedure,an iterative procedure is essentially needed to solve the uncertainty in elevation of each pixel in the SAR image.However,such an iterative procedure may suffer from the problem of divergence in shaded and serious layover areas.To investigate this problem,we performed a theoretical analysis on the convergence conditions that has not been intensively studied till now.The Range-Doppler(RD)model was simplified and then the general surface is degenerated into a planar surface.Mathematical deduction was then carried out to derive the convergence conditions and the impact factors for the convergence speed were evaluated.The theoretical findings were validated by experiments for both simulated and real scenarios. | Yuting Dong Lu Zhang Mingsheng Liao | 2016 | Geo-Spatial Information Science2016，19，4： | 1 |
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