|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Reversing cold tumors to hot: An immunoadjuvant-functionalized metalorganic framework for multimodal imaging-guided synergistic photoimmunotherapy显示文摘Immunotherapy assays using immunoadjuvants and tumor antigens could greatly increase the survival rates of patients with malignant tumors.As effective carriers,metal-organic frameworks(MOFs)have been widely utilized in cancer therapy due to their remarkable histocompatibility and low toxicity.Herein,we constructed a multimodal imaging-guided synergistic cancer photoimmunotherapy by employing a specific MOF(MIL101-NH2)as the core carrier;the MOF was dual-dressed with photoacoustic and fluorescent signal donors(indocyanine green,ICG)and immune adjuvants(cytosine-phosphate-guanine sequence,CpG)and named ICGCpG@MOF.This nanocarrier could passively target the tumor site through the EPR effect and achieve multimodal imaging(fluorescence,photoacoustic,photothermal and magnetic resonance imaging)of the tumor.Synergistic cancer photoimmunotherapy was achieved via simultaneous photodynamic and photothermal methods with 808 nm laser irradiation.ICG-CpG@MOF achieved the GSH-controlled release of immunoadjuvant into the tumor microenvironment.Furthermore,the released tumor-associated antigen along with CpG could induce the transformation of tumor cells from cold to hot by activating the immune system,which significantly enhanced tumor cytotoxicity and achieved high cure rates with minimal side-effects.This strategy utilizing multimodal imaging and synergistic cancer photoimmunotherapy provides a promising approach for the diagnosis and treatment of cancer. | Zhijin Fan Hongxing Liu Yaohua Xue Jingyan Lin Yu Fu Zhaohua Xia Dongming Pan Jian Zhang Kun Qiao Zhenzhen Zhang Yuhui Liao | 2021 | Bioactive Materials2021，6，2： | 4 |
| 2 | Crystal structure of the African swine fever virus structural protein p35 reveals its role for core shell assembly显示文摘Dear Editor,The African swine fever(ASF)is a highly contagious hemorrhagic and lethal disease in domestic pigs.The ASF outbreaks of in many Asia countries,including China,Vietnam,Mongolia,and South Korea,have posed a huge threat to the pig indusry.In the attempt to stop ASF from spreading further in China's Mainland,more than 10 million pigs have been culled since August 2018.The causative agent for ASF is a DNA virus,African swine fever virus(ASFV).Although this deadly disease has been reported in Kenya nearly one hundred years ago,there has been no vaccine for protection from ASFV infection or effective treatments to cure ASF until now. | Guobang Li Dan Fu Guangshun Zhang Dongming Zhao Mingyu Li Xue Geng Dongdong Sun Yuhui Wang Cheng Chen Peng Jiao Lin Cao Yu Guo Zihe Rao | 2020 | Protein & Cell2020，11，8： | 3 |
| 3 | Structure of African swine fever virus p15 reveals its dual role for membrane-association and DNA binding显示文摘Dear Editor, African swine fever (ASF) is a highly contagious disease of domestic pigs caused by African swine fever virus (ASFV),with mortality rates approaching 90%-100%.The first ASF case was described in 1921 in Kenya.Over the past decades, ASF has spread from Sub-Saharan Africa to Europe and Asia, and pose a huge threat to global food safety.Since the first ASF case reported in August 2018, more than 10 million pigs had been culled in China at an enormous economic loss.The risk of ASFV mutating to infect humans is extremely low.However, ASF not only threatens food security but also severely strikes the world economy in many fields, such as the global supply of the blood thinner heparin,most of which is produced from pigs.Hence, ASF is a strictly monitored infectious animal disease by OIE (World Organization for Animal Health), unfortunately, there is no approved drug or vaccine against this lethal viral disease. | Dan Fu Dongming Zhao Wei Zhang Guangshun Zhang Mingyu Li Zheng Zhang Yuhui Wang Dongdong Sun Peng Jiao Cheng Chen Yu Guo Zihe Rao | 2020 | Protein & Cell2020，11，8： | 3 |
| 4 | Lipids and membrane-associated proteins in autophagy显示文摘Autophagy is essential for the maintenance of cellular homeostasis and its dysfunction has been linked to various diseases.Autophagy is a membrane driven process and tightly regulated by membrane-associated proteins.Here,we summarized membrane lipid composition,and membrane-associated proteins relevant to autophagy from a spatiotemporal perspective.In particular,we focused on three important membrane remodeling processes in autophagy,lipid transfer for phagophore elongation,membrane scission for phago-phore closure,and autophagosome-lysosome membrane fusion.We discussed the significance of the discoveries in this field and possible avenues to follow for future studies.Finally,we summarized the membrane-associated biochemical techniques and assays used to study membrane properties,with a discussion of their applications in autophagy. | Linsen Li Mindan Tong Yuhui Fu Fang Chen Shen Zhang Hanmo Chen Xi Ma Defa Li Xiaoxia Liu Qing Zhong | 2021 | Protein & Cell2021，12，7： | 3 |
| 5 | An iterative data-driven turbulence modeling framework based on Reynolds stress representation显示文摘Data-driven turbulence modeling studies have reached such a stage that the basic framework is settled,but several essential issues remain that strongly affect the performance.Two problems are studied in the current research:(1)the processing of the Reynolds stress tensor and(2)the coupling method between the machine learning model and flow solver.For the Reynolds stress processing issue,we perform the theoretical derivation to extend the relevant tensor arguments of Reynolds stress.Then,the tensor representation theorem is employed to give the complete irreducible invariants and integrity basis.An adaptive regularization term is employed to enhance the representation performance.For the coupling issue,an iterative coupling framework with consistent convergence is proposed and then applied to a canonical separated flow.The results have high consistency with the direct numerical simulation true values,which proves the validity of the current approach. | Yuhui Yin Zhi Shen Yufei Zhang Haixin Chena Song Fu | 2022 | Theoretical & Applied Mechanics Letters2022，12，5： | 1 |
| 6 | Career adaptability, job search self-efficacy and outcomes: A three-wave investigation among Chinese university graduates显示文摘 | Yanjun Guan Hong Deng Jiaqing Sun Yanan Wang Zijun Cai Lihui Ye Ruchunyi Fu Yang Wang Shu Zhang Yuhui Li | 2013 | Journal of Vocational Behavior2013，，3： | 1 |
| 7 | PD0325901, an ERK inhibitor, enhances the efficacy of PD-1 inhibitor in non-small cell lung carcinoma显示文摘ERK pathway regulated the programmed death ligand-1(PD-L1)expression which was linked to the response of programmed death-1(PD-1)/PD-L1 blockade therapy.So it is deducible that ERK inhibitor could enhance the efficacy of PD-1 inhibitor in cancer immunotherapy.In this study,PD0325901,an oral potent ERK inhibitor,strongly enhanced the efficacy of PD-1 antibody in vitro and in vivo models in non-small cell lung carcinoma(NSCLC)cells.Mechanistically,PD0325901 or shRNA-ERK1/2 significantly downregulated the PD-L1 expression in NSCLC cells and increased the CD3+T cells infiltration and functions in tumor tissue.There was a positive correlation between the p-ERK1/2 expression and PD-L1 expression in patients with NSCLC.And the patients with low p-ERK1/2 expression were observed a high response rate of PD-1/PD-L1 blockage therapy.Our results demonstrate that PD0325901,an ERK inhibitor,can enhance the efficacy of PD-1 blockage against NSCLC in vitro and in vivo models.And the combination of ERK inhibitor such as PD0325901 and PD-1/PD-L1 blockage is a promising regimen and encouraged to be further confirmed in the treatment of patients with NSCLC. | Min Luo Yuhui Xia Fang Wang Hong Zhang Danting Su Chaoyue Su Chuan Yang Shaocong Wu Sainan An Suxia Lin Liwu Fu | 2021 | Acta Pharmaceutica Sinica B2021，11，10： | 1 |
| 8 | The Dynamic Functional Network Connectivity Analysis Framework显示文摘1. Overview The human brain is a highly dynamic system with time-varying neural activity and rapidly changing neural integration [1–3]. Research using high-temporal-resolution imaging techniques (such as electroencephalography, EEG) has identified reoccurring microstates (by characterizing changes in the power of brain activity)[4,5], which are related to spontaneous thoughts and mental processes [6]. | Zening Fu Yuhui Du Vince D. Calhoun | 2019 | Engineering2019，5，2： | 1 |
| 9 | Myosin 1D and the branched actin network control the condensation of p62 bodies显示文摘Biomolecular condensation driven by liquid-liquid phase separation(LLPS)is key to assembly of membraneless organelles in numerous crucial pathways.It is largely unknown how cellular structures or components spatiotemporally regulate LLPS and condensate formation.Here we reveal that cytoskeletal dynamics can control the condensation of p62 bodies comprising the autophagic adaptor p62/SQSTM1 and poly-ubiquitinated cargos.Branched actin networks are associated with p62 bodies and are required for their conden satio n.Myosi n 1D,a bran ched acti n-associated motor protein,drives coalesce nee of small nano scale p62 bodies into large micron-scale condensates along the branched actin network.Impairment of actin cytoskeletal networks compromises the condensation of p62 bodies and retards substrate degradation by autophagy in both cellular models and Myosin ID knockout mice.Coupling of LLPS scaffold to cytoskeleton systems may represent a general mechanism by which cells exert spatiotemporal control over phase condensation processes. | Xuezhao Feng Wanqing Du Mingrui Ding Wenkang Zhao Xirenayi Xirefu Meisheng Ma Yuhui Zhuang Xiaoyu Fu Jiangfeng Shen Jinpei Zhang Xiuying Lei Daxiao Sun Qing Xi Yiliyasi Aisa Qian Chenr Ying Li Wenjuan Wang Shanjin Huang Li Yu Pilong Li Na Mi | 2022 | Cell Research2022，32，7： | 1 |
| 10 | Bromodomain-containing proteins BRD1, BRD2, and BRD13 are core subunits of SWI/SNF complexes and vital for their genomic targeting in Arabidopsis显示文摘Switch defective/sucrose non-fermentable(SWI/SNF)chromatin remodeling complexes are multi-subunit machines that play vital roles in the regulation of chromatin structure and gene expression.However,the mechanisms by which SWI/SNF complexes recognize their target loci in plants are not fully understood.Here,we show that the Arabidopsis thaliana bromodomain-containing proteins BRD1,BRD2,and BRD13 are core subunits of SWI/SNF complexes and critical for SWI/SNF genomic targeting.These three BRDs interact directly with multiple SWI/SNF subunits,including the BRAHMA(BRM)catalytic subunit.Phenotypic and transcriptomic analyses of the brd1 brd2 brd13 triple mutant revealed that these BRDs act largely redundantly to control gene expression and developmental processes that are also regulated by BRM.Genome-wide occupancy profiling demonstrated that these three BRDs extensively colocalize with BRM on chromatin.Simultaneous loss of function of three BRD genes results in reduced BRM protein levels and decreased occupancy of BRM on chromatin across the genome.Furthermore,we demonstrated that the bromodomains of BRDs are essential for genomic targeting of the BRD subunits of SWI/SNF complexes to their target sites.Collectively,these results demonstrate that BRD1,BRD2,and BRD13 are core subunits of SWI/SNF complexes and reveal their biological roles in facilitating genomic targeting of BRM-containing SWI/SNF complexes in plants. | Yaoguang Yu Wei Fu Jianqu Xu Yawen Lei Xin Song Zhenwei Liang Tao Zhu Yuhui Liang Yuanhao Hao Liangbing Yuan Chenlong Li | 2021 | Molecular Plant2021，14，6： | 1 |
| 11 | Constructing a lithiophilic polyaniline coating via in situ polymerization for dendrite-free lithium metal anode显示文摘Metallic lithium is regarded as one of the most promising electrode materials to break through the energy density bottleneck of current commercial lithium-ion batteries.However,the practical implementation of lithium metal anode is limited by the unstable electrode interface significantly,which directly induces a low Coulombic efficiency,short cycling lifespan,and dendritic lithium growth behavior.In this study,via in situ electropolymerization,lithiophilic and conformal polyaniline layer is developed to improve the initial lithium nucleation and plating process,reducing the interface charge transfer resistance and promoting uniform lithium plating/stripping behavior.Meanwhile,the polyaniline layer exhibits good adhesion to the substrate.As a result,the Li/Cu half cell delivers a high Coulombic efficiency of 99.1%for 400 cycles at 1.0 mA·cm^(−2)with polyaniline layer.In addition,long-term stable cycling at a current density of 1.0 mA·cm^(−2)for 1300 h has been achieved for lithium metal anode.This strategy provides a new perspective for the practical lithium metal batteries. | Xiaosong Xiong Qiao Qiao Qi Zhou Xinbing Cheng Lili Liu Lijun Fu Yuhui Chen Bin Wang Xiongwei Wu Yuping Wu | 2023 | Nano Research2023，16，6： | 0 |
| 12 | Self-assembly multifunctional DNA tetrahedron for efficient elimination of antibiotic-resistant bacteria显示文摘Antibiotic resistance is a major challenge in the clinical treatment of bacterial infectious diseases.Herein,we constructed a multifunctional DNA nanoplatform as a versatile carrier for bacteria-specific delivery of clinical antibiotic ciprofloxacin(CIP)and classic nanoantibiotic silver nanoparticles(AgNP).In our rational design,CIP was efficiently loaded in the self-assembly double-bundle DNA tetrahedron through intercalation with DNA duplex,and single-strand DNA-modified AgNP was embedded in the cavity of the DNA tetrahedron through hybridization.With the site-specific assembly of targeting aptamer in the well-defined DNA tetrahedron,the bacteria-specific dual-antibiotic delivery system exhibited excellent combined bactericidal properties.With enhanced antibiotic accumulation through breaking the out membrane of bacteria,the antibiotic delivery system effectively inhibited biofilm formation and promoted the healing of infected wounds in vivo.This DNAbased antibiotic delivery system provides a promising strategy for the treatment of antibiotic-resistant infections. | Tiantian Wu Yu Fu Shuang Guo Yanqiang Shi Yuxin Zhang Zhijin Fan Bin Yang Baoquan Ding Yuhui Liao | 2024 | Aggregate2024，5，1： | 0 |
| 13 | Tyrosine promotes anthocyanin biosynthesis in pansy(Viola×wittrockiana)by inducing ABA synthesis显示文摘Viola×wittrockiana(pansy)is an important ornamental plant,particularly during winter and spring.In previous studies,we found that the tyrosine decarboxylase gene of pansy(VwTYDC)was expressed differently in blotched and non-blotched areas of pansy petals,suggesting that tyrosine may have a role in anthocyanin biosynthesis.In this study,we found that virus-induced gene silencing of VwTYDC caused an accumulation of pink pigmentation in pansy petals.Likewise,exogenous tyrosine treatment(TYRT)induced the formation of black stripes in nonblotched petal areas.Metabolome analysis indicated that the contents of two anthocyanins,cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside,increased significantly in the TYRT areas.RT-qPCR results revealed that the anthocyanin-related genes VwHCT,VwC3′H,VwCHS,and VwUGT were upregulated in the same areas.Transcriptome analysis revealed that four genes involved in the abscisic acid(ABA)biosynthesis pathway(VwNCED,VwABA2,VwAAO3,and VwCYP707A)were significantly upregulated in the same TYRT areas.ABA content was measured by ESI-HPLCMS/MS,and ABA content was significantly higher in TYRT areas than in control areas.In addition,when exogenous ABA was spread onto nonblotched petal areas,anthocyanin biosynthesis genes were upregulated just as with tyrosine.Thus,transcriptome and metabolite analyses revealed a possible novel regulatory network for anthocyanin biosynthesis in which tyrosine induces ABA synthesis and ABA then promotes anthocyanin biosynthesis in pansy petals. | Zheng Cui Jia Gu Jie Li Anjin Zhao Yingge Fu Tongxin Wang Tingge Li Xueqing Li Yuhui Sheng Ying Zhao Xiqiang Song Yang Zhou Ting Peng Jian Wang | 2022 | Tropical Plants2022，1，1： | 0 |
| 14 | A mutation in CsKTN1 for the Katanin p60 protein results in miniature plant in cucumber,Cucumis sativus L.显示文摘Katanin,a microtubule shearing protein,plays an important role in plant architecture formation.However,little is known about its mechanisms in regulating plant architecture in cucumber.In the present study,through EMS mutagenesis,we identified a novel micro-plant(mp)mutant in the North China type inbred line CCMC that may be of value for cucumber breeding.The size and number of stem cells were altered in the mp mutant.Through bulked segregant analysis(BSA)sequencing approach combined with genetic mapping,the mp locus was delimited to an interval of 130.9-kb.Multiple lines of evidence suggested that the mp mutation is due to a single nucleotide polymorphism in Csa7G435510 that is predicted to encode the Katanin p60 subunit protein(CsKTN1).The expression levels of CsKTN1 decreased significantly in all tissues except the tendril of mp mutant.Subcellular localization showed that both wild-type and mutant CsKTN1 proteins were located in cell membrane,cytoplasm and nucleus of tobacco leaf cells.The mutant protein lost part of its ability to bind and shear microtubule in vitro.These findings provide new insight into the regulatory function of microtubule-shearing protein,Katanin p60,in plant architecture of cucumber. | MengFei Song WenYuan Fu YuHui Wang Feng Cheng MengRu Zhang JinFeng Chen QunFeng Lou | 2022 | Vegetable Research2022，2，1： | 0 |
| 15 | Determination of solubility of uranium in liquid sodium显示文摘An experimental technique has been developed which overcomes the two major problems common to liquid metal solubility measurements,namely,maintaining the integrity of the samples during transfer of the liquid sodium from container to whatever device is used for analysis and detecting solute at very low concentration in liquid sodium.The solubility of uranium in liquid sodium has been measured over the temperature range 150~400℃,by equilibration and sampling technique,the solubility of uranium is approximately 0.00001%. | YANG Tongzai XING Pifeng YE Shiyou LONG Kaiming FU Zhonghua HE Yuhui JIANG Tao | 2008 | Nuclear Science and Techniques2008，19，2： | 0 |
| 16 | Mulberry leaf flavonoids activate BAT and induce browning of WAT to improve type 2 diabetes via regulating the AMPK/SIRT1/PGC-1α signaling pathway显示文摘Mulberry (Morus alba L.) leaf is a well-established traditional Chinese botanical and culinary resource. It has found widespread application in the management of diabetes. The bioactive constituents of mulberry leaf, specifically mulberry leaf flavonoids (MLFs), exhibit pronounced potential in the amelioration of type 2 diabetes (T2D). This potential is attributed to their ability to safeguard pancreatic β cells, enhance insulin resistance, and inhibit α-glucosidase activity. Our antecedent research findings underscore the substantial therapeutic efficacy of MLFs in treating T2D. However, the precise mechanistic underpinnings of MLF’s anti-T2D effects remain the subject of inquiry. Activation of brown/beige adipocytes is a novel and promising strategy for T2D treatment. In the present study, our primary objective was to elucidate the impact of MLFs on adipose tissue browning in db/db mice and 3T3-L1 cells and elucidate its underlying mechanism. The results manifested that MLFs reduced body weight and food intake, alleviated hepatic steatosis, improved insulin sensitivity, and increased lipolysis and thermogenesis in db/db mice. Moreover, MLFs activated brown adipose tissue (BAT) and induced the browning of inguinal white adipose tissue (IWAT) and 3T3-L1 adipocytes by increasing the expressions of brown adipocyte marker genes and proteins such as uncoupling protein 1 (UCP1) and beige adipocyte marker genes such as transmembrane protein 26 (Tmem26), thereby promoting mitochondrial biogenesis. Mechanistically, MLFs facilitated the activation of BAT and the induction of WAT browning to ameliorate T2D primarily through the activation of AMP-activated protein kinase (AMPK)/sirtuin 1 (SIRT1)/peroxisome proliferator-activated receptor-gamma coactivator 1α (PGC-1α) signaling pathway. These findings highlight the unique capacity of MLF to counteract T2D by enhancing BAT activation and inducing browning of IWAT, thereby ameliorating glucose and lipid metabolism disorders. As such, MLFs emerge as a prospective and innovative browning agent for the treatment of T2D. | CHENG Long SHI Lu HE Changhao WANG Chen LV Yinglan LI Huimin AN Yongcheng DUAN Yuhui DAI Hongyu ZHANG Huilin HUANG Yan FU Wanxin SUN Weiguang ZHAO Baosheng | 2023 | Chinese Journal of Natural Medicines2023，21，11： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员