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| 1 | Characterization of disinfection byproduct formation potential in 13 source waters in China显示文摘The formation potential of four trihalomethanes (THMFP) and seven haloacetic acids (HAA 7 FP) in 13 source waters taken from four major water basin areas in China was evaluated using the simulated distribution system (SDS) chlorination method.The specific ultraviolet absorbance (SUVA 254:the ratio of UV 254 to dissolved organic carbon (DOC)),which ranged between 0.9 and 5.0 L/(mg·m),showed that the organic compounds in different source waters exhibited different reactivities with chlorine.The HAA 7 FP of source waters ranged from 20 to 448 μg/L and the THMFP ranged from 29 to 259 μg/L.The HAA 7 FP concentrations were higher than the THMFP concentrations in all but one of the samples.Therefore,the risks of haloacetic acids (HAAs) should be of concern in some source waters.TCM (chloroform) and BDCM (bromodichloromethane) were the major THM constituents,while TCAA (trichloroacetic acid) and DCAA (dichloroacetic acid) were the major HAA species.Br-THM (brominated THM species) were much higher than BrHAA (brominated HAA species),and the formation of Br-DBP (Br-THM and Br-HAA) should be of concern when the bromide concentration is over 100 μg/L. | Junzhi Zhang Jianwei Yu Wei An Juan Liu Yongjing Wang Youjun Chen Jia Tai Min Yang | 2011 | Journal of Environmental Sciences2011，23，2： | 13 |
| 2 | Effects of different forms of yeast Saccharomyces cerevisiae on growth performance,intestinal development,and systemic immunity in early-weaned piglets显示文摘The present study was conducted to determine effects of different forms of yeast(Saccharomyces cerevisiae,strain Y200007) on the growth performance,intestinal development,and systemic immunity in early-weaned piglets.A total of 96 piglets(14-d old,initial average body weight of 4.5 kg) were assigned to 4 dietary treatments:(1) basal diet without yeast(Control);(2) basal diet supplemented with 3.00 g/kg live yeast(LY);(3) basal diet supplemented with 2.66 g/kg heat-killed whole yeast(HKY);and(4) basal diet supplemented with 3.00 g/kg superfine yeast powders(SFY).Diets and water were provided ad libitum to the piglets during 3-week experiment.Growth performance of piglets was measured weekly.Samples of blood and small intestine were collected at days 7 and 21 of experiment.Dietary supplementation with LY and SFY improved G:F of piglets at days 1-21 of the experiment(P<0.05) compared to Control group.Serum concentrations of growth hormone(GH),triiodothyronine(T_3),tetraiodothyronine(T_4),and insulin growth factor 1(IGF-1) in piglets at day 21 of the experiment were higher when fed diets supplemented with LY and SFY than those in Control group(P < 0.05).Compared to Control group,contents of serum urea nitrogen of piglets were reduced by the 3 yeast-supplemented diets(P< 0.05).Diets supplemented with LY increased villus height and villus-to-crypt ratio in duodenum and jejunum of piglets(P < 0.05) compared to other two groups at day 7 of the experiment.Feeding diets supplemented with LY and SFY increased(P< 0.05) serum concentrations of IgA,IL-2,and IL-6 levels in piglets compared to Control.The CD4^+/CD8^+ ratio and proliferation of T-lymphocytes in piglets fed diets supplemented with LY were increased compared to that of Control group at day 7 of the experiment(P < 0.05).In conclusion,dietary supplementation with both LY and SFY enhanced feed conversion,small intestinal development,and systemic immunity in early-weaned piglets,with better improvement in feed conversion by dietary supplementation with LY,while dietary supplementation with SFY was more effective in increasing systemic immune functions in early-weaned piglets. | Zongyong Jiang Shaoyong Wei Zhilin Wang Cui Zhu Shenglan Hu Chuntian Zheng Zhuang Chen Youjun Hu Li Wang Xianyong Ma Xuefen Yang | 2016 | Journal of Animal Science and Biotechnology2016，7，1： | 13 |
| 3 | Regulation of Actin Dynamics in Pollen Tubes:Control of Actin Polymer Level显示文摘Actin cytoskeleton undergoes rapid reorganization in response to internal and external cues. How the dynamics of actin cytoskeleton are regulated, and how its dynamics relate to its function are fundamental questions in plant cell biology. The pollen tube is a well characterized actin-based cell morphogenesis in plants. One of the striking features of actin cytoskeleton characterized in the pollen tube is its surprisingly low level of actin polymer. This special phenomenon might relate to the function of actin cytoskeleton in pollen tubes. Understanding the molecular mechanism underlying this special phenomenon requires careful analysis of actin-binding proteins that modulate actin dynamics directly. Recent biochemical and biophysical analyses of several highly conserved plant actin-binding proteins reveal unusual and un- expected properties, which emphasizes the importance of carefully analyzing their action mechanism and cellular activity. In this review, we highlight an actin monomer sequestering protein, a barbed end capping protein and an F-actin severing and dynamizing protein in plant. We propose that these proteins function in harmony to regulate actin dynamics and maintain the low level of actin polymer in pollen tubes. | Naizhi Chen Xiaolu Qu Youjun Wu Shanjin Huang | 2009 | Journal of Integrative Plant Biology2009，51，8： | 9 |
| 4 | Dietary supplementation with a high dose of daidzein enhances the antioxidant capacity in swine muscle but experts pro-oxidant function in liver and fat tissues显示文摘Background: Although isoflavones are natural dietary antioxidants, they may have toxicological effects. This study aimed to evaluate the redox system in tissues of finishing pigs by supplementation with high dose of daidzein(640 mg/kg).Results: The supplementation of high dose of daidzein for 64 d increased the activity of superoxide dismutase and total antioxidant capacity in longissimus muscle but down-regulated the expression of reactive oxygen species(ROS)-producing enzyme NADPH oxidase-2 and cyclooxygenase-2. In contrast, high-level supplementation with daidzein exerted pro-oxidant changes in back fat, abdominal fat, liver, and plasma, as reflected by increased contents of malondialdehyde, a lipid peroxidation product, in these tissues. Furthermore, daidzein supplementation resulted in higher expression of ROS-producing enzymes, including NADPH oxidase-1 and cyclooxygenase-1in liver, 5-lipoxygenase(5-LOX) in backfat and NADPH oxidase-2 both in abdominal fat and backfat. The supplementation of daidzein did not affect meat quality parameters in longissimus muscle, including marbling score,eye muscle areas, intramuscular fat, shear force, drip loss, p H and meat color.Conclusions: This experiment suggests that dietary supplementation of finishing pigs with daidzein at a high dose level improves redox status in muscle but exerts pro-oxidant effect in liver and fat tissues. | Wei Chen Xianyong Ma Yingcai Lin Yunxia Xiong Chuntian Zheng Youjun Hu Deqian Yu Zongyong Jiang | 2016 | Journal of Animal Science and Biotechnology2016，7，3： | 8 |
| 5 | A genome-wide analysis of SWEET gene family in cotton and their expressions under different stresses显示文摘Background: The SWEET(Sugars will eventually be exported transporters) gene family plays multiple roles in plant physiological activities and development process. It participates in reproductive development and in the process of sugar transport and absorption, plant senescence and stress responses and plant-pathogen interaction. However,thecomprehensive analysis of SWEET genes has not been reported in cotton.Results: In this study, we identified 22, 31, 55 and 60 SWEET genes from the sequenced genomes of Gossypium arboreum, G. raimondii, G. hirsutum and G. barbadense, respectively. Phylogenetic tree analysis showed that the SWEET genes could be divided into four groups, which were further classified into 14 sub-clades. Further analysis of chromosomal location, synteny analysis and gene duplication suggested that the orthologs showed a good collinearity and segmental duplication events played a crucial role in the expansion of the family in cotton. Specific MtN3_slv domains were highly conserved between Arobidopsis and cotton by exon-intron organization and motif analysis. In addition, the expression pattern in different tissues indicated that the duplicated genes in cotton might have acquired new functions as a result of sub-functionalization or neo-funtionalization. The expression pattern of SWEET genes showed that the different genes were induced by diverse stresses. The identification and functional analysis of SWEET genes in cotton may provide more candidate genes for genetic modification.Conclusion: SWEET genes were classified into four clades in cotton. The expression patterns suggested that the duplicated genes might have experienced a functional divergence. This work provides insights into the evolution of SWEET genes and more candidates for specific genetic modification, which will be useful in future research. | ZHAO Lanjie YAO Jinbo CHEN Wei LI Yan L Youjun GUO Yan WANG Junyi YUAN Li LIU Ziyang ZHANG Yongshan | 2018 | Journal of Cotton Research2018，1，2： | 6 |
| 6 | Effects of soil nutrients and climate factors on belowground biomass in an alpine meadow in the source region of the Yangtze-Yellow rivers, Tibetan Plateau of China显示文摘Improving our knowledge of the effects of environmental factors(e.g.soil conditions,precipitation and temperature) on belowground biomass in an alpine grassland is essential for understanding the consequences of carbon storage in this biome.The object of this study is to investigate the relative importance of soil nutrients and climate factors on belowground biomass in an alpine meadow in the source region of the Yangtze and Yellow rivers,Tibetan Plateau.Soil organic carbon(SOC),total nitrogen(TN) and total phosphorous(TP) contents and belowground biomass were measured at 22 sampling sites across an alpine meadow on the Tibetan Plateau.We analyzed the data by using the redundancy analysis to determine the main environmental factors affecting the belowground biomass and the contribution of each factor.The results showed that SOC,TN and TP were the main factors that influenced belowground biomass,and the contribution of SOC,TN and TP on biomass was in the range of 47.87%–72.06% at soil depths of 0–30 cm.Moreover,the combined contribution of annual mean temperature(AMT) and mean annual precipitation(MAP) on belowground biomass ranged from 0.92% to 4.10%.A potential mechanism for the differences in belowground biomass was caused by the variations in soil nitrogen and phosphorous,which were coupled with SOC.A significant correlation was observed between MAP and soil nutrients(SOC,TN and TP) at the soil depth of 0–10 cm(P<0.05).We concluded that precipitation is an important driving force in regulating ecosystem functioning as reflected in variations of soil nutrients(SOC,TN and TP) and dynamics of belowground biomass in alpine grassland ecosystems. | WANG Haiming SUN Jian LI Weipeng WU Jianbo CHEN Youjun LIU Wenhui | 2016 | Journal of Arid Land2016，8，6： | 5 |
| 7 | Unexpected complexity of multidrug resistance in the mcr-1-harbouring Escherichia coli显示文摘Dear Editor,The discovery that the mobile colistin resistance gene(mcr-1)is encoded by plasmids and is prevalent in food animals and human beings worldwide(Hasman et al.,2015;Liu et al.,2015)has challenged greatly our traditional idea that polymyxin(consisting of two isoforms:polymyxin B and polymyxin E(colistin))acts as an ultimate line of refuge in the clinical treatment against the severe infections by the multidrug-resistant Gram-negative pathogens(Nation et al.,2015;Paterson and Harris,2015).To make | Rongsui Gao Yue Li Jingxia Lin Chen Tan Youjun Feng | 2016 | Science China(Life Sciences)2016，59，7： | 5 |
| 8 | Global Planted Forest Development: Opportunities,Challenges and Policy Choices显示文摘In recent decades, there has seen a dramatic expansion of global planted forest area and their great impact on human life. It is reported in Forest Resource Assessment 2010 that the current area of global planted forests is about 264 million ha, representing only 7% of the total forest area but able to meet the two thirds of the global demand for logs. Planted forests can not only provide timber, fiber, fuel and non-wood forest products, but also contribute to carbon sequestration, restoration of degraded land, landscape rehabilitation and watershed protection. Besides, planted forests can also provide the recreation and amusement for people. In such sense, in the coming decades, planted forests, driven by various factors such as social- economic conditions, market, consumers' demand and new technologies, will have good opportunities for development, and will also increase their contribution to achieving the series of development objectives worldwide. In the meanwhile, the development of planted forests is also constrained by the factors like policies, laws, regulations, technologies and funds, and therefore faced with the challenges in terms of eco- environment preservation, economic development and protection of community interests. Based on the long- term monitoring and research of planted forest development as well as the field study of planted forests abroad, the paper identified the opportunities and challenges in the development of global planted forests through such methods as the SWOT, policy analysis and the others, and proposed the policy choices for promoting the sustainable development of planted forests: 1) There is a need to further understand the functions of planted forests and the importance of the degraded land restoration; 2) A good institutional guarantee and investment environment should be created for the planted forest development; 3) The advanced concepts and methods in terms of the planted forests management should be actively promoted and applied; 4) There is a need to put more focus on environment management and social responsibility; 5) The intensive management of planted forests needs to rely on science and technology support; 6) The international cooperation should be strengthened to jointly facilitate the sustainable development of planted forests. | HE Youjun CHEN Jie LI Zhiyong Research Institute of Forestry Policy and Information, Chinese Academy of Forestry, Beijing 100091,P.R. China | 2010 | Chinese Forestry Science and Technology2010，9，2： | 4 |
| 9 | Image of Landscapes in Ancient Water Towns——Case Study on Zhouzhuang and Tongli of Jiangsu Province显示文摘Researchers in several disciplines and fields agree that the image establishing has both perceptual and affec-tive evaluations.The paper comparatively analyzes the image of landscapes in ancient water towns held by 1619 tourists after the investigations of Zhouzhuang and Tongli of Jiangsu Province.Based on the elements of the image of urban landscapes,the perceptual/cognitive image of tourist landscapes in water towns is developed including path,node,landmark,important courtyard,district and edge.Particularly the important courtyard plays the great role on the per-ceptive image of tourists,however,the perceptual/cognitive images of the district and the edge are obviously weak.Meanwhile,the finding showed that the affective images of tourist landscapes in Zhouzhuang differ with those of Tongli by the method of paired comparison.The main reason is that the affective images are influenced by the impressive scenery spots.Furthermore,the commercialization of streets and pollution of rivers are easy to be functioned negatively. | LI Yabing ZHANG Jie CHEN Youjun | 2006 | Chinese Geographical Science2006，16，4： | 4 |
| 10 | A Highly Efficient Agrobacterium-Mediated Method for Transient Gene Expression and Functional Studies in Multiple Plant Species显示文摘Although the use of stable transformation technology has led to great insight into gene function,its application in high-throughput studies remains arduous.Agro-infiltration have been widely used in species such as Nicotiana benthamiana for the rapid detection of gene expression and protein interaction analysis,but this technique does not work efficiently in other plant species,including Arabidopsis thaliana.As an efficient high-throughput transient expression system is currently lacking in the model plant species A.thaliana,we developed a method that is characterized by high efficiency,reproducibility,and suitability for transient expression of a variety of functional proteins in A.thaliana and 7 other plant species,including Brassica oleracea,Capsella rubella,Thellungiella salsuginea,Thellungiella halophila,Solanum tuberosum,Capsicum annuum,and N.benthamiana.Efficiency of this method was independently verified in three independent research facilities,pointing to the robustness of this technique.Furthermore,in addition to demonstrating the utility of this technique in a range of species,we also present a case study employing this method to assess protein–protein interactions in the sucrose biosynthesis pathway in Arabidopsis. | Youjun Zhang Moxian Chen Beata Siemiatkowska Mitchell Rey Toleco Yue Jing Vivien Strotmann Jianghua Zhang Yvonne Stahl Alisdair R.Fernie | 2020 | Plant Communications2020，1，5： | 4 |
| 11 | Influence of metal ions on sulfonamide antibiotics biochemical behavior in fiber coexisting system显示文摘Metal ions and fiber are common compounds in the livestock and poultry manure,which will affect the fate of organic compounds in aqueous environment. However,limited research has addressed the effect of coexisting metal ions and fiber on the biodegradation of sulfonamide antibiotics. Accordingly, a compositing study was performed to assess the effect of metal ions(Fe3+and Cu2+) on the biodegradation of sulfadimethoxine sodium salt(SDM) in the presence of fiber. The enhanced adsorption of SDM onto fiber in the presence of metal ions can be attributed to the π+–π electron donor acceptor(EDA) interaction. The microbial(Phanerochaete chrysosprium) could easily attach onto fiber forming attached microbial, and the degradation rates of SDM of immobilized bacteria in the presence of Fe3 +were 100%, which were significantly higher than those of free bacteria(45%). This study indicates that Fe3 +and fiber could enhance the biodegradation of SDM. Fiber acts as adsorbent, carrier, and substrate which enhanced the removal of SDM. | Lan Zhang Ruihuan Chen Yun Liu Youjun Deng Zhongpei Li Yuanhua Dong | 2019 | Journal of Environmental Sciences2019，31，6： | 3 |
| 12 | Diversified variants of the mcr-1-carrying plasmid reservoir in the swine lung microbiota显示文摘Dear Editor,Emergence of bacteria with multiple drug resistance is a major problem in global public health.Polymyxin comprising polymyxin E(colistin)and polymyxin B is generally recognized as the last-resort antibiotics with | Zhencui Li Chen Tan Jingxia Lin Youjun Feng | 2016 | Science China(Life Sciences)2016，59，9： | 3 |
| 13 | RNA interference-mediated silencing of vATPase subunits A and E affect survival and development of the 28-spotted ladybeetle,Henosepilachna vigintioctopunctata显示文摘RNA interference(RNAi)has emerged as a powerful tool for developing novel management strategies for controlling insect pests.The 28-spotted ladybeetle,Henosepilachna vigintioctopunctata is one of the most important pests attacking solanaceous plants in Asia.In this study,the potential of dietary RNAi to manage H.vigintioctopunctata was investigated using both in vitro synthesized and bacterially expressed double-stranded RNAs(dsRNAs)of HvvATPase A and HvvATPase E.The expression levels of HvvATPase A and HvvATPase E were higher in Malpighian tubules than in other tissue types.The silencing of HvvATPase A and HvvATPase E led to significant mortality in H.vigintioctopunctata larvae.In addition,the ingestion of HvvATPase A and HvvATPase E significantly deterred feeding behavior and subsequently arrested the development of H.vigintioctopunctata.Notably,the bacterially expressed dsRNAs consistently caused higher mortality in larvae and adults.Finally,the nontarget effects of the dsRNAs of H.vigintioctopunctata on the predatory ladybeetle Propylaea japonica were evaluated.P.japonica 1st instar larvae were administered vATPase A and vATPase E dsRNAs from H.vigintioctopunctata and P.japonica under the worst-case scenario,in which dsGFP served as negative control.There were significant effects of dsHvvATPase A on P.japonica at the transcriptional level but not at the organismal level,whereas dsHvvATPase E did not effect P.japonica at either the transcriptional or the organismal level.Collectively,the results of the study suggest that HvvATPase A and HvvATPase E can act as novel molecular targets for the control of H.vigintioctopunctata. | Wei Guo Mujuan Guo Chunxiao Yang Zhuoqi Liu Shimin Chen Jing Lu Baoli Qiu Youjun Zhang Xuguo Zhou Huipeng Pan | 2021 | Insect Science2021，28，6： | 3 |
| 14 | Discovery of multi-drug resistant,MCR-1 and ESBL-coproducing ST117 Escherichia coli from diseased chickens in northeast China显示文摘An endemic multi-drug resistant ST117 E. coli isolate coproducing MCR-1 and 3 ESBL loci was, for the first time, detected from diseased chicken, Liaoning Province, in Northeast China, from 2011 to 2012. Wholegenome sequencing revealed 5 unique plasmids, namely pHXH-1, pHXH-2, pHXH-3, pHXH-4 and pHXH-5). Among them, pHXH1 and pHXH4 encode ESBL, and pHXH-5 mediates MCR-1 colistin resistance. The results indicate that the potentially-national dissemination of MCR-1-positive pathogens with pan-drug resistance proceeds via food chains. | Sijia Ding Xiaohu Han Jun Li Weifan Gao Zeliang Chen Youjun Feng | 2018 | Science Bulletin2018，63，16： | 2 |
| 15 | Elementary Experiences,Problems and Countermeasures of County Forestry Economy显示文摘County forestry economy is a strong power for regional green economic development,and an important way to improve farmers' income and promote living environment in urban and rural areas. According to the investigation of ten typical counties or cities with forestry industrial advantage,this paper sums up the elementary experiences of county forestry economy,analyzes its prominent problems. Countermeasures are brought forward to improve the development of county forestry economy. | Shaozhi CHEN Baomin FAN Rong ZHAO Youjun HE Decheng ZHANG | 2013 | Asian Agricultural Research2013，5，3： | 2 |
| 16 | In situ time-resolved FTIRS study of adsorption and oxidation of ethylene glycol on Pt(100) electrode显示文摘Adsorption and oxidation of ethylene glyco(EG) on Pt(100) electrode were studied by in situ timeresolved FTIRS (TRFTIRS). The TRFTIR spectra recordedat 0.10 V illustrate that an IR band appears near 2050 cm?at t > 5 s, corresponding to linearly bonded CO formed indissociative adsorption of EG. The TRFTIR results haveconfirmed also that CO species are distributed uniformly onPt(100) surface. Another band appears near 2342 cm?1 at > 70 s, associating with IR absorption of CO2 produced inthe direct oxidation of EG. With the increase of electrodepotential, the direct oxidation of EG becomes gradually themain reaction. When the potential is above 0.40 V, the oxidation of EG occurs mainly via the reactive intermediates, i.especies containing –COOH determined by in situ TRFTIRS. | FAN Youjun ZHOU Zhiyou FAN Chunjie ZHEN Chunhua CHEN Shengpei SUN Shigang | 2005 | Chinese Science Bulletin2005，50，18： | 2 |
| 17 | Cosmological parameter fittings with the BICEP2 data显示文摘Combining the latest Planck,Wilkinson Microwave Anisotropy Probe(WMAP),and baryon acoustic oscillation(BAO)data,we exploit the recent cosmic microwave background(CMB)B-mode power spectra data released by the BICEP2 collaboration to constrain the cosmological parameters of the ACDM model,especially the primordial power spectra parameters of the scalar and the tensor modes,ns,s,r,nt.We obtain constraints on the parameters for a lensed ACDM model using the Markov Chain Monte Carlo(MCMC)technique,the marginalized 68%bounds are r=0.03070.09140.1043,ns=0.00610.00610.9617,s=0.01050.00970.0175,nt=0.45150.45790.5198.We find that a blue tilt for nt is favored slightly,but it is still well consistent with flat or even red tilt.Our r value is slightly smaller than the one obtained by the BICEP group,in that we permit nt as a free parameter without imposing the single-field slow roll inflation consistency relation.When we impose this relation,then r=0.04460.06090.2130.For most other parameters,the best fit values and measurement errors are not altered significantly by the introduction of the BICEP2 data. | WU FengQuan LI YiChao LU YouJun CHEN XueLei | 2014 | Science China(Physics,Mechanics & Astronomy)2014，57，8： | 2 |
| 18 | Study on Design of Interplex Method for GNSS Signal显示文摘 | FENG Youjun WANG Zhong CHEN Dandan | 2014 | China Communications2014，11，A02： | 2 |
| 19 | Effect of powders refinement on the tribological behavior of Ni-based composite coatings by laser cladding显示文摘 | Lingqian Wang Jiansong Zhou Youjun Yu Chun Guo Jianmin Chen | 2012 | Applied Surface Science2012，，17： | 2 |
| 20 | Direct control of store-operated calcium channels by ultrafast laser显示文摘Ca^(2+) channels are essential to cell birth,life,and death.They can be externally activated by optogenetic tools,but this requires robust introduction of exogenous optogenetic genes for expression of photosensitive proteins in biological systems.Here we present femtoSOC,a method for direct control of Ca^(2+)channels solely by ultrafast laser without the need for optogenetic tools or any other exogenous reagents.Specifically,by focusing and scanning wavelength-tuned low-power femtosecond laser pulses on the plasma membrane for multiphoton excitation,we directly induced Ca^(2+) influx in cultured cells.Mechanistic study reveals that photoexcited flavins covalently bind cysteine residues in Orai1 via thioether bonds,which facilitates Orai1 polymerization to form store-operated calcium channels(SOCs)independently of STIM1,a protein generally participating in SOC formation,enabling all-optical activation of Ca^(2+) influx and downstream signaling pathways.Moreover,we used femtoSOC to demonstrate direct neural activation both in brain slices in vitro and in intact brains of living mice in vivo in a spatiotemporal-specific manner,indicating potential utility of femtoSOC. | Pan Cheng Xiaoying Tian Wanyi Tang Juan Cheng Jin Bao Haipeng Wang Sisi Zheng Youjun Wang Xunbin Wei Tunan Chen Hua Feng Tian Xue Keisuke Goda Hao He | 2021 | Cell Research2021，31，7： | 2 |
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