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| 1 | Structure and Anti-influenza A(H1N1) Virus Activity of Three Polysaccharides from Eucheuma denticulatum显示文摘Three polysaccharides(EW,EH and EA) were prepared from a red alga Eucheuma denticulatum by sequential extraction with cold water,hot water and sodium hydroxide water solution.Their monosaccharide compositions,relative molecular mass and structural characterization were determined by gas chromatography,high performance 1iquid chromatography,fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy methods.EW was hybrid ι/κ/ν-carrageenan(70 ι/17κ/13ν-carrabiose),EH was mainly ι-carrageenan,and EA was mainly α-1,4-Glucan(88%) but mixed with small amount of ι-carrageenan(12%).The relative molecular mass of EW,EH and EA was 480,580 and 510 kDa,respectively.The anti-influenza A(H1N1) virus activity of these three polysaccharides was evaluated using the Madin-Darby canine kidney cells model.EW showed good anti-H1N1 virus activity,its IC 50 was 276.5 μg mL-1,and the inhibition rate to H1N1 virus was 52% when its concentration was 250 μg mL-1.The IC 50 of ι-carrageenan EH was 366.4 μg mL-1,whereas EA showed lower anti-H1N1 virus activity(IC 50 >430 μg mL-1).Available data obtained give positive evidence that the hybrid carrageenan EW from Eucheuma denticulatum can be used as potential anti-H1N1 virus inhibitor in future. | YU Guangli LI Miaomiao WANG Wei] LIU Xin ZHAO Xiaoliang LV Youjing LI Guangsheng JIAO Guangling ZHAO Xia | 2012 | Journal of Ocean University of China2012，11，4： | 5 |
| 2 | Methods for calculating glacier area and length in a mountainous area based on remote-sensing data and a digital elevation model显示文摘In a mountainous region, the glacier area and length extracted form the satellite imagery data is the projected area and length of the land surface, which can't be representative of the reality; there are always some errors. In this paper, the methods of calculating glacier area and length calculation were put forward based on satellite imagery data and a digital elevation model (DEM). The pure pixels and the mixed pixels were extracted based on the linear spectral un-mixing approach, the slop of the pixels was calculated based on the DEM, then the area calculation method was presented. The projection length was obtained from the satellite imagery data, and the elevation differences was calculated from the DEM. The length calculation method was presented based on the Pythagorean theorem. For a glacier in the study area of western Qilian Mountain, northwestern China, the projected area and length were 140.93 km2 and 30.82 km, respectively. This compares with the results calculated by the methods in this paper, which were 155.16 km2 and 32.11 km respectively, a relative error of the projected area and length extracted from the LandSat Thematic Mapper (TM) image directly reach to -9.2 percent and -4.0 percent, respectively. The calculation method is more in accord with the practicality and can provide reference for some other object's area and length monitoring in a mountainous region. | JunZhan Wang YouJing Zhang WeiMin Zhang JianJun Qu Chen Chen | 2010 | Research in Cold and Arid Regions2010，2，4： | 4 |
| 3 | Preparation of κ-carra-Oligosaccharides with Microwave Assisted Acid Hydrolysis Method显示文摘A rapid method of microwave assisted acid hydrolysis was established to prepare κ-carra-oligosaccharides. The optimal hydrolysis condition was determined by an orthogonal test. The degree of polymerization(DP) of oligosaccharides was detected by high performance thin layer chromatography(HPTLC) and polyacrylamide gel electrophoresis(PAGE). Considering the results of HPTLC and PAGE, the optimum condition of microwave assisted acid hydrolysis was determined. The concentration of κ-carrageenan was 5 mg m L-1; the reaction solution was adjusted to p H 3 with diluted hydrochloric acid; the solution was hydrolyzed under microwave irradiation at 100 for 15℃ min. Oligosaccharides were separated by a Superdex 30 column(2.6 cm × 90 cm) using AKTA Purifier UPC100 and detected with an online refractive index detector. Each fraction was characterized by electrospray ionization mass spectrometry(ESI-MS). The data showed that odd-numbered κ-carra-oligosaccharides with DP ranging from 3 to 21 could be obtained with this method, and the structures of the oligosaccharides were consistent with those obtained by traditional mild acid hydrolysis. The new method was more convenient, efficient and environment-friendly than traditional mild acid hydrolysis. Our results provided a useful reference for the preparation of oligosaccharides from other polysaccharides. | LI Guangsheng ZHAO Xia LV Youjing LI Miaomiao YU Guangli | 2015 | Journal of Ocean University of China2015，14，2： | 3 |
| 4 | IL-17A Plays a Critical Role in the Pathogenesis of Liver Fibrosis through Hepatic Stellate Cell Activation显示文摘 | Zhongming Tan Xiaofeng Qian Runqiu Jiang Qianghui Liu Youjing Wang Chen Chen Xuehao Wang Bernhard Ryffel Beicheng Sun | 2013 | The Journal of Immunology2013，，4： | 2 |
| 5 | Why Are hyperlinks To Business Web Sites created?content analysis显示文摘 | VaughanL YouJ Kipp M | 2006 | Scientometrics2006，67，2： | 1 |
| 6 | Radiation therapy versus chemotherapy as initial treatment for localized nasal natural killer (NK)/T-cell lymphoma: a single institute survey in Taiwan显示文摘 | YOUJ Y CHIK II YANGM H | 2004 | Ann Oncol2004，15，： | 1 |
| 7 | Effects of cadmium stress on the germination growth of seedling and physiological and biochemical characters of wheat [J]显示文摘 | YangYL(杨颖丽) WangWR(王文瑞) YouJ(尤佳) etal | 2012 | 西南师范大学学报2012，48，3： | 1 |
| 8 | Photothermal -chemotherapywith doxorubicin -loaded hollow gold nanospheres: Aplatform for near -infrared light -trigged drug release 显示文摘 | YouJ Zhar|g R Zhang)GD | 2012 | Journal of controlled release2012，156，2： | 1 |
| 9 | Comparison and combination of several stress relief methods for cubic boron nitride films deposited by ion beam assisted deposition 显示文摘 | FAN Y M ZHANG X W YOUJ B | 2009 | Surface and Coatings Technology2009，203，1011： | 1 |
| 10 | Radiation therapy versus chemotherapy as initial treatment for localized nasal natural killer(NK)/T-cell lymphoma: a single institute survey in Taiwan显示文摘 | YOUJ Y CHI K H YANG M H | 2004 | Ann Oncol2004，15，： | 1 |
| 11 | Thespecificeffectofmetronomeguidanceonthequalityofonepersoncardiopulmonaryresuscitationandrescuerfatigue显示文摘 | ChungTN KimSW YouJS etal | 2012 | JEmerg-Med2012，43，6： | 1 |
| 12 | Effects of laser shock processing on stress corrosion cracking susceptibility of AZ31B magnesium alloy显示文摘 | ZhangYK YouJ LuJZ etal | 2010 | Surface and Coatings Technology2010，204，24： | 1 |
| 13 | 显示文摘 | ZENGHua-jin LIANGHui-li YOUJing etal(曾华金 梁会丽 游静 等) | 2013 | 发光学报2013，34，3： | 1 |
| 14 | Novel fermentation process smengthening strengthening strategy for production of propionic acid and vitamin B12 by Propionibacterium freudenreichii显示文摘 | WANG Peng JIAO Youjing LILT Shonxin | 2014 | Joural of Industrial Microbiology Biotechnology2014，41，： | 1 |
| 15 | Use of the delta P1 approximation for recovery of optical absorption, scattering, and asymmetry coefficients in turbid media显示文摘 | Hayakawa C K HillBY YouJ S | 2004 | Appl Opt2004，43，： | 1 |
| 16 | An inverse relationship between KAIl expression,invasive ability,and MMP-2 expression and activity in bladder cancer cell lines显示文摘 | YouJ Madigan MC Rowe A | 2012 | Urol Oncol2012，30，4： | 1 |
| 17 | Comparison of ro- botic adrenalectomy with traditional laparoseopic adre- nalectomy with a lateral transperitoneal approach: a single-surgeon experience显示文摘 | YouJ Y LeeHY SonGS etal | 2013 | Int J Med Robot2013，9，3： | 1 |
| 18 | Online continuous flow ultrasonic extraction coupled with high performance liq uid chromatographic separation for determination of the fla- vonoids from root of Scutellaria baicalensis Georgi显示文摘 | YOUJ Y GAO S Q JIN H Y | 2010 | Jour nal of Chromatography A2010，1217，12： | 1 |
| 19 | Searching for potential bio- markers of cisplatin resistance in human ovarian cancer using a label-free LC/MS-based protein quantification method显示文摘 | Fitzpatrick DP YouJS Bemis KG | 2007 | Proteomics Clin Appl2007，1，3： | 1 |
| 20 | Experi ments on anisotropic etching ofSi in TMA H显示文摘 | Youj s Ki md Huhj y | 2001 | Sol Energy Mater Sol Cells2001，66，： | 1 |
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