Preparation of κ-carra-Oligosaccharides with Microwave Assisted Acid Hydrolysis Method

查看全文 作　　者：LI [1,2]Guangsheng;ZHAO [1,2]Xia;LV [1,2]Youjing;LI [1,2]Miaomiao;YU [1,2]Guangli

机构地区：[1]Key Laboratory of Marine Drugs,Ministry of Education;[2]Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy,Ocean University of China

出　　处：《Journal of Ocean University of China》索引2015年第14卷第2期,共5页

基　　金：supported by the Special Fund for Marine Scientific Research in the Public Interest (201005024);NSFC-Shandong Joint Fund for Marine Science Research Centers (U1406402);Qingdao Science & Technology Project (11-2-2-1-hy);National Science & Technology Support Program of China (2013BAB 01B02) 摘　　要：A rapid method of microwave assisted acid hydrolysis was established to prepare κ-carra-oligosaccharides. The optimal hydrolysis condition was determined by an orthogonal test. The degree of polymerization(DP) of oligosaccharides was detected by high performance thin layer chromatography(HPTLC) and polyacrylamide gel electrophoresis(PAGE). Considering the results of HPTLC and PAGE, the optimum condition of microwave assisted acid hydrolysis was determined. The concentration of κ-carrageenan was 5 mg m L-1; the reaction solution was adjusted to p H 3 with diluted hydrochloric acid; the solution was hydrolyzed under microwave irradiation at 100 for 15℃ min. Oligosaccharides were separated by a Superdex 30 column(2.6 cm × 90 cm) using AKTA Purifier UPC100 and detected with an online refractive index detector. Each fraction was characterized by electrospray ionization mass spectrometry(ESI-MS). The data showed that odd-numbered κ-carra-oligosaccharides with DP ranging from 3 to 21 could be obtained with this method, and the structures of the oligosaccharides were consistent with those obtained by traditional mild acid hydrolysis. The new method was more convenient, efficient and environment-friendly than traditional mild acid hydrolysis. Our results provided a useful reference for the preparation of oligosaccharides from other polysaccharides. 关键词：酸水解法微波辅助寡糖制备聚丙烯酰胺凝胶电泳 HPTLC ESI-MS PAGE

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Preparation of κ-carra-Oligosaccharides with Microwave Assisted Acid Hydrolysis Method

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