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| 1 | Loss of clusterin both in serum and tissue correlates with the tumorigenesis of esophageal squamous cell carcinoma via proteomics approaches显示文摘AIM: To identify the differentially secreted proteins or polypeptides associated with tumorigenesis of esophageal squamous cell carcinoma (ESCC) from serum and to find potential tumor secreted biomarkers.METHODS: Proteins from human ESCC tissue and its matched adjacent normal tissue; pre-surgery and postsurgery serum; and pre-surgery and normal control serum were separated by two-dimensional electrophoresis (2-DE)to identify differentially expressed proteins. The silverstained 2-DE were scanned with digital ImageScanner and analyzed with ImageMaster 2D Elite 3.10 software. A cluster of protein spots differentially expressed were selected and identified with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). One of the differentially expressed proteins, clusterin, was downregulated in cancer tissue and pre-surgery serum, but it was reversed in post-surgery serum. The results were confirmed by semi-quantitative reverse-transcription (RT)-PCR and western blot.RESULTS: Comparisons of the protein spots identified on the 2-DE maps from human matched sera showed that some proteins were differentially expressed, with most of them showing no differences in composition, shape or density.Being analyzed by MALDI-TOF-MS and database searching,clusterin was differentially expressed and down-regulated in both cancer tissue and pre-surgery serum compared with their counterparts. The results were also validated by RTPCR and western blot.CONCLUSION: The differentially expressed clusterin may play a key role during tumorigenesis of ESCC. The 2DE-MS based proteomic approach is one of the powerful tools for discovery of secreted markers from peripheral. | Li-YongZhang Wan-TaoYing You-ShengMao Hong-ZhiHe YuLiu Hui-XinWang FangLiu KunWang De-ChaoZhang YingWang MinWu Xiao-HongQian Xiao-HangZhao | 2003 | World Journal of Gastroenterology2003，9，4： | 26 |
| 2 | Expression of PDCD5 , a novel apoptosis related protein, in human osteoarthritic cartilage显示文摘AIM: To investigate the expression features of PDCD5 (programmed cell death 5 protein) in osteoarthritic andnormal human cartilage, and speculate on its potential functions in the pathogenesis of osteoarthritis (OA).METHODS: Articular cartilage specimens were obtained from 30 patients with OA and 16 healthy patients at thetime of arthroplasty. Expression of PDCD5 was detected by flow cytometry, immunofluorescence, RT-PCR andimmunohistochemical analysis. RESULTS: Enhanced expression and nuclear accumulation of PDCD5 in OAchondrocytes were found. PDCD5-positive chondrocytes were mainly distributed in the superficial and deep zonesof OA tissue sections, as opposed to, in the superficial and middle regions of normal healthy tissue sections.CONCLUSION: Since apoptotic chondrocyte death occurs more frequently in OA cartilage than in normal healthycartilage and PDCD5 is an apoptosis-related protein, the different expression patterns of PDCD5 in OA cartilagefrom that in normal healthy cartilage indicate that PDCD5 is involved in the pathogenesis of OA. | Ai-xinCHENG Si-quanLOU Hao-weiZHOU YingWANG Da-longMA | 2004 | Acta Pharmacologica Sinica2004，25，5： | 15 |
| 3 | Translocation of annexin I from cellular membrane to the nuclear membrane in human esophageal squamous cell carcinoma显示文摘AIM: To investigate the alteration of the annexin I subcellular localization in esophageal squarnous cell carcinoma (ESCC)and the correlation between the translocation and the tumorigenesis of ESCC.METHODS: The protein localization of annexin I was detected in both human ESCC tissues and cell line via the indirect immunofluorescence strategy.RESULTS: In the normal esophageal epithelia the annex in I was mainly located on the plasma membrane and formed a consecutive typical trammels net. Annexin I protein also expressed dispersively in cytoplasm and the nuclei without specific localization on the nuclear membrane. In esophageal cancer annexin I decreased very sharply with scattered disappearance on the cellular membrane, however it translocated and highly expressed on the nuclear membrane,which was never found in normal esophageal epithelia. In cultured esophageal cancer cell line annexin I protein was also focused on the nuclear membrane, which was consistent with the result from esophageal cancer tissues.CONCLUSION: This observation suggests that the translocation of annexin I protein in ESCC may correlate with the tumorigenesis of the esophageal cancer. | YuLiu Hui-XinWang NingLu You-ShengMao FangLiu YingWang Hai-RongZhang KunWang MinWu Xiao-HangZhao | 2003 | World Journal of Gastroenterology2003，9，4： | 10 |
| 4 | Diminution of toxic copper accumulation in toxic milk mice modeling Wilson disease by embryonic hepatocyte intrasplenic transplantation显示文摘AIM: To observe the therapeutic effect of intrasplenic transplantation with embryonic hepatocytes on amelioration of hereditary copper accumulation in toxic milk (TX) mouse modeling Wilson disease. METHODS: Donor hepatocytes were harvested from 14-d fetal liver of a pregnant homogeneous DL mouse. These cells were successively cultured, labeled with fluorescein dye Hoechst 33342 for 24 h, and sequentially infused into the spleen parenchyma of the recipient TX mice. No host immunosuppression measures were taken. Two and four weeks after transplantation, the recipients were killed for routine histologic investigation and immunohistochemistry study up to 4 wk after transplantation. The serum copper and ceruloplasmin concentrations of the recipient mice were determined by graphite furnace atomic absorption spectroscopy.RESULTS: In the following 2nd and 4th wk after transplantation, the donor hepatocytes could be visualized in the livers of 47.3% recipients. The serum ceruloplasmin and copper concentrations increased by 1.6-fold after 2 wk and 2.0-fold times after 4 wk respectively, which ultimately rose from about 30% of the normal level to nearly 60%(P<0.01). The hepatic copper concentration decreased 7.2%, 4 wk after transplantation. Pathologic examination showed that there were many actively proliferative hepatocyte precursor cells with specific embryonic hepatocyte marker AFP migrated into hepatic sinusoidsof the recipients. A large number of cells carrying hepatocytes marker and albumin were observed in the recipient spleen tissues.CONCLUSION: Embryonic hepatocytes are capable of differentiating into mature hepatocytes in vivo. After transplantation, the hereditary abnormalities of copper metabolism in TX mice could be corrected partially by intrasplenic transplantation of homogeneous embryonic hepatocytes. | ZhuShi Xiu-LingLiang Bing-XunLu Su-YuePan XiChen Qi-QiangTang YingWang FanHuang | 2005 | World Journal of Gastroenterology2005，11，24： | 8 |
| 5 | Blood Oxygenation Level-dependent Magnetic Resonance Imaging of Breast Cancer: Correlation with Carbonic Anhydrase IX and Vascular Endothelial Growth Factor显示文摘 | YingWang Min Liu Mu-Lan Jin | 2017 | Chinese Medical Journal2017，，1： | 7 |
| 6 | Early Detection of Regional and Global Left Ventricular Myocardial Function Using Strain and Strain-rate Imaging in Patients with Metabolic Syndrome显示文摘 | QinWang Qi-Wei Sun Dan Wu Ming-Wu Yang Rong-Juan Li Bo Jiang Jiao Yang Zhi-An Li YingWang Ya Yang | 2015 | Chinese Medical Journal2015，，2： | 6 |
| 7 | Contribution of C3d-P28 repeats to enhancement of immune responses against HBV-preS2/S induced by gene immunization显示文摘AIM: To investigate whether P28 derived from C3d can enhance the immune response to HBV-preS2/S induced by directly injection of naked plasmids containing variable repeats of P28 and HBV-preS2/S in fusion form.METHODS: One to four copies of C3d-P28 coding gene,amplified by PCR and modified by restriction endonuclease sdigestion, were subcloned into a eukaryotic expression vector pVAON33 to construct pVAON33-P28, pVAON33-P28.2, pVAON33-P28.3 and pVAON33-P28.4 (pVAON33-P28.[1-4]). HBV-preS2/S coding sequence was then introduced into the pVAON33-P28.[1-4] and identified by both PCR and DNA sequencing. BALB/c mice were primed by intramuscular gene immunization with 100 μg different recombinant plasmids on day 0 and were boosted by subcutaneous inoculation with HBsAg protein (1 μg) 12 wk post-priming. The levels and avidity of specific IgG in sera collected at the indicated times from each group were determined by ELISA and NaSCN-displacement ELISA,respectively.RESULTS: HBsAg specific antibody response was elicited in groups primed with plasmids pVAON33-S2/S-P28.[1-4] and pVAON33-S2/S. However, the response against HBsAg in the groups primed with pVAON33-S2/S-P28.[1-4] was significantly higher than that in pVAON33-S2/S group, the highest level of the specific antibody response was observed in the groups primed with pVAON33-S2/S-P28.4 (P<0.01).After secondary immunization with specific antigen, the acceleration of antibody levels was significantly higher and faster in the mice primed with DNA expressing preS2/S-P28 fusions than that with DNA expressing preS2/S only (P<0.05).Interestingly, mice primed with DNA expressing preS2/S-P28.4 fusions maintained the highest levels of anti-HBs antibodies in all animals. The avidity assay showed that the avidity index (AI) collected at 18 wk from mice primed with pVAON33-S2/S-P28.3 and pVAON33-S2/S-P28.4 were significantly higher than that from preS2/S-DNA vaccinated mice (P<0.01).CONCLUSION: Different repeats of C3d-P28 can enhance both humoral immune response and avidity maturation of specific antibodies induced by gene immunization, in which four copies of C3d-P28 may be necessary to achieve the most modest antibody response. | Li-XinWang WeiXu Qing-DongGuan Yi-WeiChu YingWang Si-DongXiong | 2004 | World Journal of Gastroenterology2004，10，14： | 4 |
| 8 | Distribution of Helicobacter pylori in north China显示文摘AIM: To compare the distribution of virulence-associatedgenotypes of Helicobacter pylori(H pylori) in two areas of north China with different gastric cancer risk and furthermore probe into the pathogenicity of the bacterium. METHODS: Gastric biopsies were taken from 355 subjects from Zhuanghe, a high risk area of gastric cancer, and 136 subjects from Shenyang, a low risk area of gastric cancer. A total of 149 H pylori strains isolated from these patients were studied by PCR for differences in the genotypes of cagA, vac A, and iceA.RESULTS: In patients with high risk for gastric cancer, higher frequencies of vacA s1 or s1m1b genotypes were found as compared to those from the low risk area. CONCLUSION: There is significantly different distribution of H pylori genotypes between Zhuanghe and Shenyang areas in north China. | Yue-HuaGong YingWang YuanYuan | 2005 | World Journal of Gastroenterology2005，11，23： | 4 |
| 9 | A Rapid and Simple Approach to Preparation of Monoclonal Antibody Based on DNA Immunization显示文摘Inoculation with purified specific protein is usually the first step for preparation of monoclonal antibody (mAb).But it is quite difficult to obtain pure proteins especially with natural structures. Here we attempt to replace the protein inoculation with DNA immunization in the preparation of mAb. The eukaryotic expression vectors pcDNA3-PreS2/S and pVAX-PreS2/S encoding the HBV M protein were constructed and prepared for DNA immunization. Female BALB/c mice developed a well antibody response to the target antigen after muscle injection with corresponding plasmids. The mice with effective antibodies induced were used for preparation of mAb. We found the mice immunized with three administrations of pcDNA3-PreS2/S and boosted by intrasplenic injection with the same plasmid could be exploited for preparation of mAb. And positive hybridoma cell 2D3 that can secrete specific mAb was cloned and analyzed. Our studies demonstrate that gene immunization may provide a convenient and efficient way to prepare mAbs. | YiNi KeMa JingNi XiujuanZheng YingWang SidongXiong | 2004 | Cellular & Molecular Immunology2004，1，4： | 3 |
| 10 | Relationship between Expression of Cell Adhesion Molecules and the Metastatic Mechanism in Invasive Micropapillary Carcinoma of the Breast显示文摘OBJECTIVE To explore the expression and the function of cell adhesion molecules in invasive micropapillary carcinoma (IMPC) of the breast, and to investigate the metastatic mechanism of IMPC. METHODS The expression of E-cadherin, α-catenin and β-catenin was detected by imrnunohistochemical staining in 64 cases of IMPC, and compared with that of invasive ductal carcinoma (IDC). RESULTS E-cadherin and β-catenin were mainly expressed on the cell membrane of tumors, and cc-catenin was expressed in the cytoplasm and/or on the cell membrane. The expression of E-cadherin in IMPC was significantly higher than that in IDC. Furthermore, the expression of E-cadherin was mainly on the intercellular contact surface of the tumor cell clusters in IMPC, while that on the outer surface of the tumor cell clusters decreased or could not be detected. The degree of lymph nodes metastases in IMPC was significantly higher than that in IDC. The co-expressions of α-catenin and β-catenin in cases of lymph nodes metastases along with the expression of E-cadherin in IMPC were significantly higher than that in IDC. CONCLUSIONS These findings indicated that the adhesiveness of the intercellular contact surfaces of tumor clusters in IMPC was strong, while that of the outer surface of tumor clusters was decreased or lost. It is suggested that the adhesive characteristic of the cells in IMPC might play an important role in its higher metastatic potential. | YuFan LiFu RonggangLang YingWang XiangchengZhi BaocunSun | 2004 | Chinese Journal of Clinical Oncology2004，1，1： | 2 |
| 11 | FLUID-BASED SIMULATION APPROACH FOR HIGH VOLUME CONVEYOR TRANSPORTATION SYSTEMS显示文摘High volume conveyor systems in distribution centers have very large footprint and can handle large volumes and hold thousands of items. Traditional discrete-event cell-based approach to simulate such networks becomes computationally challenging. An alternative approach, in which the traffic is represented by segments of fluid flow of different density instead of individual packages, is presented in this paper to address this challenge. The proposed fluid-based simulation approach is developed using a Hybrid Petri Nets framework. The underlying model is a combination of an extension of a Batches Petri Nets (BPN) and a Stochastic Petri Nets (SPN). The extensions are in the inclusion of random elements and relaxation of certain structural constraints. Some adaptations are also made to fit the target system modeling. The approach is presented with an example. | YingWANG ChenZHOU | 2004 | Systems Science and Systems Engineering2004，13，3： | 2 |
| 12 | Specific siRNA Downregulated TLR9 and Altered Cytokine Expression Pattern in Macrophage after CpG DNA Stimulation显示文摘Bacterial CpG DNA or synthetic oligonucleotides (ODNs) that contain unmethylated CpG motifs (CpG ODN) can directly activate antigen-presenting cells (APCs) to secrete various cytokines through the intracellular receptor TLR9. Cytokine profiles elicited by the actions of stimulatory CpG DNA on TLR9 expressed APCs are crucial to the subsequent immune responses. To date, cytokine profiles in APCs upon CpG ODN stimulation in vitro are not fully investigated. In the present study, vector-based siRNA was used to downregulate TLR9 expression. Cytokine profiles were observed in murine macrophage cell line RAW264.7 transfected with TLR9-siRNA plasmid upon CpG ODN stimulation. We found that not all the cytokine expressions by the macrophage were decreased while TLR9 was downregulated. IL-12, TNF-α, IFN-γ and IL-1β expressions were significantly decreased, but IL-6,IFN-β and IL-10 expressions were not affected. Interestingly, the level of IFN-α was even increased. This alteration of cytokines produced by TLR9-downregulated APCs upon CpG ODN stimulation might indicate that the role of CpG DNA is more complicated in the pathogenesis and prevention of diseases. Cellular & Molecular Immunology.2005;2(2):130-135. | BinQiao BaohuaLi XiuliYang HongyongZhang YiweiChu YingWang SidongXiong | 2005 | Cellular & Molecular Immunology2005，2，2： | 2 |
| 13 | Food Safety Risks and Contributing Factors of Cronobacter spp.显示文摘Cronobacter species are a group of Gram-negative opportunistic pathogens,which cause meningitis,sep-ticemia,and necrotizing enterocolitis in neonates and infants,with neurological sequelae in severe cases.Interest in Cronobacter has increased significantly in recent years due to its high virulence in children.In this review,we summarize the current understanding of the prevalence of Cronobacter species in several important food types.We discuss the response mechanisms enabling persistence in adverse growth con-ditions,as well as its pathogenicity.We emphasize the food safety concerns caused by Cronobacter and subsequent control methods and clinical treatments. | Na Ling Xiuting Jiang Stephen Forsythe Danfeng Zhang Yizhong Shen Yu Ding Juan Wang Jumei Zhang Qingping Wu Yingwang Ye | 2022 | Engineering2022，8，5： | 2 |
| 14 | Research on Remote Monitoring and Fault Diagnosis Technology of Numerical Control Machine显示文摘Based on the internet technology,it has become possible to complete remote monitoring and fault diagnosis for the numerical control machine.In order to capture the micro-shock signal induced by the incipient fault on the rotating parts,the reso- nance demodulation technology is utilized in the system.As a subsystem of the remote monitoring system,the embedded data acquisi- tion instrument not only integrates the demodulation board but also complete the collection and preprocess of monitoring data from different machines.Furthermore,through connecting to the internet,the data can be transferred to the remote diagnosis center and data reading and writing function can be finished in the database.At the same time,the problem of the IP address floating in the dial-up of web server is solved by the dynamic DNS technology.Finally,the remote diagnosis software developed on the Lab VIEW platform can analyze the monitoring data from manufacturing field.The research results have indicated that the equipment status can be monitored by the system effectively. | ZHANG Jianyu~1 GAO Lixin~1 CUI Lingli~1 LI Xianghui~2 WANG Yingwang~2 1.Key Laboratory.of Advanced Manufacturing Technology,Beijing University of Technology,Beijing 100022,China 2.Tangshan Iron and Steel Corp.LTD,Tangshan 063000,China | 2006 | 武汉理工大学学报2006，28，S2： | 1 |
| 15 | Analysis of a consensus fragment in ERIC-PCR fingerprinting of Enterobacter sakazakii显示文摘 | Yingwang Ye Qingping Wu Lin Yao Xiaohui Dong Kui Wu Jumei Zhang | 2009 | International Journal of Food Microbiology2009，，2： | 1 |
| 16 | Down-regulation of p21 contributes to apoptosis induced by HPV E6 in human mammary epithelial cells显示文摘 | Xueli Fan Yingwang Liu Jason J. Chen | 2005 | Apoptosis2005，，1： | 1 |
| 17 | A compari- son of polymerase chain reaction and international organization for standardization methods for determination of Enterobacter sakazakii contamination of infant formulas from Chinese mainland markets显示文摘 | YE YINGWANG WU QINGPING LIN YAO | 2009 | Foodlorne Pathogens Disease2009，6，10： | 1 |
| 18 | Correlates of Sexual Lifestyles Among Older Adults Living With HIV in China: Findings From the Sexual Well-being (SWELL) Study显示文摘Sexual lifestyles are closely related to overall human health and well-being.Few studies have focused on sexual lifestyles among older adults living with HIV(OALHIV),especially in low-and middle-income countries.This study is a part of the sexual well-being among older adults in China(SWELL)study,which is a multicenter cross-sectional study focused on sexual health among older adults aged 50 years and older.Participants were 680 OALHIV(≥50 years old)from the SWELL study conducted from June 2020 to December 2022.Data were collected through one-on-one interviews.We used logistic regression to assess the correlates of sexual activity and sexual satis-faction.Among all participants,37.1%were sexually active.Being older,male,in a steady relationship and employed were associated with being sexually active.The prevalence of sexual satisfaction was 69.8%among sexually active OALHIV.Being homosexual and reporting a better general health status were associated with sexual satisfaction.The OALHIV who have depressive symptoms were less likely to report sexual satisfaction.To support holistic healthy aging among OALHIV,it is important for healthcare providers to be educated about the importance of enquiring about sexual activity,satisfaction and problems and addressing concerns while conveying sex-positive attitudes during clinical reviews,as these are still critical aspects of their health and well-being. | Bingyi Wang Xin Peng Leiwen Fu Xinyi Li Tongxin Shi Bowen Liang Tian Tian Thomas Fitzpatrick Zhen Lu Weijie Zhang JunyeBian Xin Xiao YingWang Yunlong Ao Lin Ouyang YongCai Maohe Yu Guohui Wu Linghua Li Xiaojun Meng Huachun Zou | 2023 | Infectious Microbes & Diseases2023，5，3： | 1 |
| 19 | CuO Nanospheres Based Nonenzymatic Glucose Sensor显示文摘 | EliotReitz WenzhaoJia MichaelGentile YingWang YuLei | 2008 | Electroanalysis2008，，22： | 1 |
| 20 | Analysis of a consensus fragment in ERIC-PCR fingerprinting of Enter-obacter sakazakii 显示文摘 | Yingwang Y Qingping W Lin Y | 2009 | Food Microbiology2009，132，23： | 1 |
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