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| 1 | Torsional optomechanical cooling of a nanofiber显示文摘We demonstrate the optomechanical cooling of a tapered optical nanofiber by coupling the polarization of light to the mechanical angular momentum of the system. The coupling is enabled by birefringence in the fiber and does not make use of an optical resonator. We find evidence for cooling in the distribution of thermally driven amplitude fluctuations and the noise spectrum of the torsional modes. Our proof-of-principle demonstration shows cavity-less cooling of the torsional degree of freedom of a macroscopically extended nanofiber. | Dianoiang Su Pablo Solano Jeffrey D.Wack Luis A.Orozco Yanting Zhao | 2022 | Photonics Research2022，10，2： | 1 |
| 2 | Deep learning quantified mucus-tumor ratio predicting survival of patients with colorectal cancer using whole-slide images显示文摘Background:In colorectal cancer(CRC),mucinous adenocarcinoma differs from other adenocarcinomas in gene-phenotype,morphology,and prognosis.However,mucinous components are present in a large number of adenocarcinomas,and the prognostic value of mucus proportion has not been investigated.Artificial intelligence provides a way to quantify mucus proportion on whole-slide images(WSIs)accurately.We aimed to quantify mucus proportion by deep learning and further investigate its prognostic value in two CRC patient cohorts.Methods:Deep learning was used to segment WSIs stained with hematoxylin and eosin.Mucus-tumor ratio(MTR)was defined as the proportion of mucinous component in the tumor area.A training cohort(N=419)and a validation cohort(N=315)were used to evaluate the prognostic value of MTR.Survival analysis was performed using the Cox proportional hazard model.Result:Patients were stratified tomucus-low andmucus-high groups,with 24.1%as the threshold.In the training cohort,patients with mucus-high had unfavorable outcomes(hazard ratio for high vs.low 1.88,95%confidence interval 1.18–2.99,P=0.008),with 5-year overall survival rates of 54.8%and 73.7%in mucus-high and mucus-lowgroups,respectively.The resultswere confirmed in the validation cohort(2.09,1.21–3.60,0.008;62.8%vs.79.8%).The prognostic value of MTR was maintained in multivariate analysis for both cohorts.Conclusion:The deep learning quantified MTR was an independent prognostic factor in CRC.With the advantages of advanced efficiency and high consistency,our method is suitable for clinical application and promotes precision medicine development. | Ke Zhao Lin Wu Yanqi Huang Su Yao Zeyan Xu Huan Lin Huihui Wang Yanting Liang Yao Xu Xin Chen Minning Zhao Jiaming Peng Yuli Huang Changhong Liang Zhenhui Li Yong Li Zaiyi Liu | 2021 | Precision Clinical Medicine2021，4，1： | 0 |
| 3 | IQGAP3 promotes the progression of glioma as an immune and prognostic marker显示文摘Background:IQGAP3 plays a crucial role in regulating cell proliferation,division,and cytoskeletal organization.Abnormal expression of IQGAP3 has been linked to various tumors,but its function in glioma is not well understood.Methods:Various methods,including genetic differential analysis,single-cell analysis,ROC curve analysis,Cox regression,Kaplan-Meier analysis,and enrichment analysis,were employed to analyze the expression patterns,diagnostic potential,prognostic implications,and biological processes involving IQGAP3 in normal and tumor tissues.The impact of IQGAP3 on immune infiltration and the immune microenvironment in gliomas was evaluated using immunofluorescence.Additionally,the cBioPortal database was used to analyze copy number variations and mutation sites of IQGAP3.Experimental validation was also performed to assess the effects of IQGAP3 on glioma cells and explore underlying mechanisms.Results:High IQGAP3 expression in gliomas is associated with an unfavorable prognosis,particularly in wild-type IDH and 1p/19q non-codeleted gliomas.Enrichment analysis revealed that IQGAP3 is involved in regulating the cell cycle,PI3K/AKT signaling,p53 signaling,and PLK1-related pathways.Furthermore,IQGAP3 expression may be closely related to the immunosuppressive microenvironment of glioblastoma.BRD-K88742110 and LY-303511 are potential drugs for targeting IQGAP3 in anti-glioma therapy.In vitro experiments showed that downregulation of IQGAP3 inhibits the proliferation and migration of glioma cells,with the PLK1/PI3K/AKT pathway potentially playing a crucial role in IQGAP3-mediated glioma progression.Conclusion:IQGAP3 shows promise as a valuable biomarker for diagnosis,prognosis,and immunotherapeutic strategies in gliomas. | XIAOFENG GAO JUANJUAN GE XUZHENG GAO NA MEI YANTING SU SHIGANG SHAN WENBIN QIAN JIANGHENG GUAN ZHENWANG ZHANG LONG WANG | 2024 | Oncology Research2024，32，4： | 0 |
| 4 | Efficient TurboID-based proximity labelling method for identifying terminal sialic acid glycosylation in living cells显示文摘TurboID,a proximity labelling method based on mutant biotin ligase,is an efficient new technique for recognizing protein-protein interactions and has been successfully applied to living cells.Sialic acid is typically the terminal monosaccharide attached to many glycoproteins and plays many important roles in many biological processes.However,the lack of enrichment methods for terminal sialic acid glycosylation in vivo hinders the identification and analysis of this glycosylation.Here,we introduce TurboID to identify terminal sialic acid glycosylation in living cells.SpCBM,the carbohydrate-binding domain of sialidase from Streptococcus pneumoniae,is fused with TurboID and overexpressed in HeLa cells.After streptavidin-based purification and detection by mass spectrometry,31 terminal sialic acid N-glycosylated sites and 1359 putative terminal sialic acid glycosylated proteins are identified,many of which are located in the cytoplasm and nucleus. | Wu Liu Yunfeng Long Yongfen Bao Yang Li Mengxue Deng Xiuyi Yang He Zhu Yanting Su | 2022 | Acta Biochimica et Biophysica Sinica2022，54，12： | 0 |
| 5 | Synergistic effects of AAGL and anti-PD-1 on hepatocellular carcinoma through lymphocyte recruitment to the liver显示文摘Objective:Therapy for hepatocellular carcinoma(HCC)is a major challenge,and targeted therapies provide only a modest benefit in terms of overall survival.Treatment with antibodies to programmed cell death protein 1(PD-1)/PD-L1 can restore the functions of tumor-infiltrating T cells in HCC and has shown clinical efficacy in 20%of patients with advanced HCC.Novel approaches are urgently needed to treat HCC and to augment the efficacy of immunotherapy.Methods:Tumor-bearing mice were treated with Agrocybe aegerita galectin(AAGL)alone or in combination with anti-PD-1,and the tumor sizes and lifespans of mice were determined.Transcriptome analysis,cytokine analysis,flow cytometry analysis of the number and proportion of immune cell subsets in the liver and spleen,and molecular and cellular analyses of tumors were used to define the underlying mechanisms.Results:AAGL significantly inhibited the growth of liver tumors in a dose-dependent manner.Furthermore,AAGL increased the expression of multiple cytokines and chemokines in tumor-bearing mouse livers;this effect was associated with the activation and migration of T cells and macrophages,in agreement with the in vitro results.Importantly,the aggregation of T cells and macrophages induced by AAGL in tumor-bearing mouse livers clearly enhanced the response to PD-1 blockade immunotherapy.Conclusions:The results showed that AAGL induced the activation and migration of lymphocytes to the liver,and that the combination of AAGL and anti-PD-1 may be a promising strategy for HCC treatment. | Xiangdong Ye Xueqing Wang Wenhui Yu Qing Yang Yan Li Yanxia Jin Yanting Su Jiaqi Song Bo Xu Hui Sun | 2021 | Cancer Biology & Medicine2021，18，4： | 0 |
| 6 | Optomechanical feedback cooling of a 5 mm long torsional mode显示文摘We report three orders of magnitude optical cooling of the fundamental torsional mode of a 5 mm long,550 nm diameter optical nanofiber.The rotation of the nanofiber couples to the polarization of guided laser fields.We use a weak laser probe to monitor the rotation and use feedback to modulate the polarization of an auxiliary drive laser providing torque.Our results present a tool for the optomechanical control of large-scale torsional resonators,with metrological applications and potential implications for studying macroscopic objects in quantum states. | DIANQIANG SU YUAN JIANG PABLO SOLANO LUIS A.OROZCO JOHN LAWALL YANTING ZHAO | 2023 | Photonics Research2023，11，12： | 0 |
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