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| 1 | Characterization of microRNAs in serum: a novel class of biomarkers for diagnosis of cancer and other diseases显示文摘在各种各样的纸巾的 microRNAs (miRNAs ) 的 Dysregulated 表示与许多疾病被联系了,包括癌症。这里,我们证明 miRNAs 在象老鼠,老鼠,牛的胎儿,小牛,和马那样的人和另外的动物的浆液和血浆是在场的。在浆液的 miRNAs 的层次在一样的种类的个人之中稳定、可再现、一致。采用 Solexa,我们定序健康中国题目的所有浆液 miRNAs 并且分别地在男、女的题目发现超过 100 和 91 浆液 miRNAs。我们也为肺癌症, colorectal 癌症,和糖尿病识别了浆液 miRNAs 的特定的表示模式,提供证据那浆液 miRNAs 为各种各样的疾病包含指纹。癌症特定的浆液 miRNAs 由 Solexa 获得了的二个非小的房间肺进一步在 75 个健康施主和 152 个癌症病人的独立审判被验证,用量的反向的抄写聚合酶链反应试金。通过这些分析,我们断定浆液 miRNAs 能为各种各样的癌症和另外的疾病的察觉用作潜在的 biomarkers。 | Xi Chen Yi Ba Lijia Ma Xing Cai Yuan Yin Kehui Wang Jig ang Guo Yujing Zhang Jiangning Chen Xing Guo Qibin Li Xiaoying Li Wenjing Wang Yan Zhang Jin Wang Xueyuan Jiang Yang Xiang Chen Xu Pingping Zheng Juanbin Zhang Ruiqiang Li Hongjie Zhang Xiaobin Shang Ting Gong Guang Ning Jun Wang Ke Zen Junfeng Zhang Chen-Yu Zhang | 2008 | Cell Research2008，18，10： | 975 |
| 2 | Spatiotemporal characteristics, patterns, and causes of land-use changes in China since the late 1980s显示文摘Land-use/land-cover 变化(LUCC ) 有连接到人和自然相互作用。瓷器 Land-Use/cover 数据集(CLUD ) 从 1980 年代末在 5 年的间隔定期被更新到 2010,与基于 Landsat TMETM+ 图象的标准过程。陆地使用动态区域化方法被建议分析主要陆地使用变换。在国家规模的陆地使用变化的空间与时间的特征,差别,和原因然后被检验。主要调查结果如下被总结。越过中国的陆地使用变化(LUC ) 在最后 20 年(19902010 ) 里在空间、时间的特征显示了一个重要变化。农田变化的区域在南方减少了并且在北方,而是仍然是的全部的区域增加了几乎未改变。回收农田从东北被转移到西北。布满建筑物陆地很快膨胀了,主要在东方被散布,并且逐渐地展开到中央、西方的中国。树林首先减少了,然后增加但是荒芜的区域是反面。草地继续减少。在中国的 LUC 的不同空间模式被发现在之间迟了第 20 世纪并且早第 21 世纪。原版 13 个 LUC 地区在一些地区被边界的变化由 15 个单位代替。包括的这些变化(1 ) 的主要空间特征加速的扩大布满建筑物在 Huang-Huai-Hai 区域,东南的沿海的区域,长江的中流区域,和四川盆登陆;(2 ) 从东北中国和东方内部蒙古在北方转移了陆地开垦到绿洲在西北中国的农业区域;(3 ) 从在到稻的东北中国的喂雨的农田的连续转变回答;并且(4 ) 为在内部蒙古,黄土高原,和西南的多山的区域的南部的农业牧剧的交错群落的格林工程的谷物的有效性。在最后二十年,尽管在北方的气候变化在农田影响了变化,政策规定和经济驱动力仍然是越过中国的 LUC 的主要原因。在第 21 世纪的第一十年期间,在陆地使用模式驾驶了变化的人为的因素从单程的陆地开发转移了强调到开发和保存。动态区域化方法被用来在单位的 zoning 边界,地区的内部特征,和生长和减少的空间模式分析变化。结果揭示了变化过程的模式,也就是 LUC 的过程和在在不同阶段的特征的地区性的差别。地区的生长和减少在这动态 LUC zoning,在统一边界的变化,和在前者和后者十年之间的变化紧张的特征期间被检验。在为在不同类型和陆地使用的不同区域的变化的陆地使用和原因的模式和过程之间的其他的转变的模式被探索。 | LIU Jiyuan KUANG Wenhui ZHANG Zengxiang XU Xinliang QIN Yuanwei NING Jia ZHOU Wancun ZHANG Shuwen LI Rendong YAN Changzhe WU Shixin SHI Xuezheng JIANG Nan YU Dongsheng PAN Xianzhang CHI Wenfeng | 2014 | Journal of Geographical Sciences2014，24，2： | 245 |
| 3 | Modeling study of regional severe hazes over mid-eastern China in January 2013 and its implications on pollution prevention and control显示文摘The Nested Air Quality Prediction Model System(NAQPMS)was used to investigate the temporal and spatial variations of PM2.5over tropospheric central eastern China in January 2013.The impact of regional transport and its implications on pollution prevention and control were also examined.Comparison between simulated and observed PM2.5showed NAQPMS was able to reproduce the evolution of PM2.5during heavy haze episodes.The results indicated that regional transport of PM2.5played an important role in regional haze episodes in the city cluster including Hebei,Beijing and Tianjin(HBT).The cross-city clusters transport outside HBT and transport among cities inside HBT contributed 20%–35%and 26%–35%of PM2.5as compared with local emission,in HBT respectively.To meet the Air Quality Standards for Grade II,90%,90%and65%of emissions would have to be cut down in Hebei,Tianjin and Beijing,if non-control strategy was taken in the surrounding city clusters of HBT.This implicated that control of emissions in one city cluster is not sufficient to reduce regional haze events,and joint efforts among city clusters are essential.Besides regional transports,two-way feedback between boundary-layer evolution and PM2.5also significantly contributed to the formation of heavy hazes,which contributed 30%of monthly average PM2.5concentration in HBT. | WANG ZiFa LI Jie WANG Zhe YANG WenYi TANG Xiao GE BaoZhu YAN PinZhong ZHU LiLi CHEN XueShun CHEN HuanSheng WAND Wei LI JianJun LIU Bing WANG XiaoYan WAND Wei ZHAO YiLin LU Ning SU DeBin | 2014 | Science China Earth Sciences2014，57，1： | 106 |
| 4 | Service-aware 6G:An intelligent and open network based on the convergence of communication,computing and caching显示文摘With the proliferation of the Internet of Things(IoT),various services are emerging with totally different features and requirements,which cannot be supported by the current fifth generation of mobile cellular networks(5G).The future sixth generation of mobile cellular networks(6G)is expected to have the capability to support new and unknown services with changing requirements.Hence,in addition to enhancing its capability by 10–100 times compared with 5G,6G should also be intelligent and open to adapt to the ever-changing services in the IoT,which requires a convergence of Communication,Computing and Caching(3C).Based on the analysis of the requirements of new services for 6G,this paper identifies key enabling technologies for an intelligent and open 6G network,all featured with 3C convergence.These technologies cover fundamental and emerging topics,including 3C-based spectrum management,radio channel construction,delay-aware transmission,wireless distributed computing,and network self-evolution.From the detailed analysis of these 3C-based technologies presented in this paper,we can see that although they are promising to enable an intelligent and open 6G,more efforts are needed to realize the expected 6G network. | Yiqing Zhou Ling Liu Lu Wang Ning Hui Xinyu Cui Jie Wu Yan Peng Yanli Qi Chengwen Xing | 2020 | Digital Communications and Networks2020，6，3： | 57 |
| 5 | A life support-based comprehensive treatment regimen dramatically lowers the in-hospital mortality of patients with fulminant myocarditis: a multiple center study显示文摘Fulminant myocarditis(FM) has unacceptable high mortality. This study aimed to evaluate the therapeutic efficacy of a life support-based comprehensive treatment regimen(LSBCTR), a completely novel treatment regimen, for FM. A total of 169 FM patients recruited from January 2008 to December 2018 were divided into two groups: patients receiving LSBCTR(81 cases),which includes(i) mechanical life support(positive pressure respiration, intra-aortic balloon pump with or without extracorporeal membrane oxygenation),(ii) immunomodulation therapy using sufficient doses of glucocorticoids and immunoglobulins, and(iii) application of neuraminidase inhibitors, and those receiving conventional treatment(88 cases). The endpoints were in-hospital death and heart-transplantation. Of all the population, 44 patients(26.0%) died in hospitals. Inhospital mortality was 3.7%(3/81) for LSBCTR group and 46.6%(41/88) for traditional treatment(P<0.001). Early application of LSBCTR, mechanical life support, neuraminidase inhibitors, and immunomodulation therapy significantly contributed to reduction in in-hospital mortality. This study describes a novel treatment regimen for FM patients that dramatically reduces inhospital mortality. Its generalization and clinical application will efficiently save lives although further optimization is needed.This study offers an insight that virus infection induced inflammatory waterfall results in cardiac injury and cardiogenic shock and is the therapeutic target. | Sheng Li Shengyong Xu Chenze Li Xiao Ran Guanglin Cui Mengying He Kun Miao Chunxia Zhao Jiangtao Yan Rutai Hui Ning Zhou Yan Wang Jiangang Jiang Jing Zhang Daowen Wang | 2019 | Science China(Life Sciences)2019，62，3： | 43 |
| 6 | Programmable time-domain digital-coding metasurface for non-linear harmonic manipulation and new wireless communication systems显示文摘Optical non-linear phenomena are typically observed in natural materials interacting with light at high intensities, and they benefit a diverse range of applications from communication to sensing. However,controlling harmonic conversion with high efficiency and flexibility remains a major issue in modern optical and radio-frequency systems. Here, we introduce a dynamic time-domain digital-coding metasurface that enables efficient manipulation of spectral harmonic distribution. By dynamically modulating the local phase of the surface reflectivity, we achieve accurate control of different harmonics in a highly programmable and dynamic fashion, enabling unusual responses, such as velocity illusion. As a relevant application, we propose and realize a novel architecture for wireless communication systems based on the time-domain digital-coding metasurface, which largely simplifies the architecture of modern communication systems, at the same time yielding excellent performance for real-time signal transmission. The presented work, from new concept to new system, opens new pathways in the application of metamaterials to practical technology. | Jie Zhao Xi Yang Jun Yan Dai Qiang Cheng Xiang Li Ning Hua Qi Jun Chen Ke Guo Dong Bai Shuo Liu Shi Jin Andrea Alù Tie Jun Cui | 2019 | National Science Review2019，6，2： | 46 |
| 7 | Cardiomyocyte overexpression of miR-27b induces cardiac hypertrophy and dysfunction in mice显示文摘最近的研究开始在心脏的肥大和机能障碍的致病揭示了 microRNAs (miRNAs ) 的关键角色。在这研究,我们测试了是否转变生长 factor-β(TGF-β) 调整 miRNA 在心脏的肥大和心失败(HF ) 的发展起了一个枢轴的作用。我们观察到 miR-27b 是在 cardiomyocyte 特定的 Smad4 猛烈老鼠的心的 upregulated,它开发了心脏的肥大。在 vitro,实验证明 miR-27b 表示能被 TGF-β 禁止; 1 并且它的 overexpression 支持了 hypertrophic 房间生长,当 miR-27b 抑制导致了 hypertrophic 房间的抑制时,生长由 phenylephrine (PE ) 引起了处理。而且,有 miR-27b 的 cardiomyocyte 特定的 overexpression 的转基因的老鼠的分析表明 miR-27b overexpression 是足够的导致心脏的肥大和机能障碍。我们验证了 peroxisome 激活 proliferator 的 receptor-γ(PPAR-γ) 作为在 cardiomyocyte 的 miR-27b 的一个直接目标。一致地, miR-27b 转基因的老鼠显著地显示了 PPAR-γ 的底层;比控制鼠标。而且,在用在一只 pressure-overload-induced 老鼠的特定的 antagomir 的 miR-27b 的 vivo silencing, HF 的模型增加了心脏的 PPAR-γ表示,稀释心脏的肥大和机能障碍。我们的学习的结果表明那 TGF-β 1-regulated miR-27b 涉及心脏的肥大的规定,并且为心脏病作为一个有效治疗学的目标验证 miR-27b。 | Jian Wang Yao Song Yan Zhang Han Xiao Qiang Sun Ning Hou Shuilong Guo Youliang Wang Kaiji Fan Dawei Zhan Lagabaiyila Zha Yang Cao Zhenhua Li Xuan Cheng Youyi Zhang Xiao Yang | 2012 | Cell Research2012，22，3： | 36 |
| 8 | Fibrinogen-like protein 2 fibroleukin expression and its correlation with disease progression in murine hepatitis virus type 3-induced fulminant hepatitis and in patients with severe viral hepatitis B显示文摘AIM: To evaluate the expression of fibrinogenlike protein 2 (fgl2) and its correlation with disease progression in both mice and patients with severe viral hepatitis.METHODS: Balb/cJ or A/J mice were infected intraperitoneally (ip) with 100 PFU of murine hepatitis virus type 3 (MHV-3), liver and serum were harvested at 24, 48, and 72 h post infection for further use. Liver tissues were obtained from 23 patients with severe acute chronic (AOC) hepatitis B and 13 patients with mild chronic hepatitis B. Fourteen patients with mild chronic hepatitis B with cirrhosis and 4 liver donors served as normal controls. In addition, peripheral blood mononuclear cells (PBMC) were isolated from 30 patients (unpaired) with severe AOC hepatitis B and 10 healthy volunteers as controls. Procoagulant activity representing functional prothrombinaseactivity in PBMC and white blood cells was also assayed. A polyclonal antibody against fgl2 was used to detect the expression of both mouse and human fgl2 protein in liver samples as well as in PBMC by immunohistochemistry staining in a separate set of studies. Alanine aminotransferase (ALT) and total bilirubin (TBil) in serum were measured to assess the severity of liver injury.RESULTS: Histological changes were found in liver sections 12-24 h post MHV-3 infection in Balb/cJ mice.In association with changes in liver histology, marked elevations in serum ALT and TBil were observed. Mouse fgl2 (mfgl2) protein was detected in the endothelium of intrahepatic veins and hepatic sinusoids within the liver 24 h after MHV-3 infection. Liver tissues from the patients with severe AOC hepatitis B had classical pathological features of acute necroinflammation. Human fgl2 (hfgl2)was detected in 21 of 23 patients (91.30%)with severe AOC hepatitis B, while only 1 of 13 patients(7.69%) with mild chronic hepatitis B and cirrhosis had hfgl2 mRNA or protein expression. Twenty-eight of thirty patients (93.33%) with severe AOC hepatitis B and 1of 10 with mild chronic hepatitis B had detectable hfgl2expression in PBMC. No hfgl2 expression was found either in the liver tissue or in the PBMC from normal donors. There was a positive correlation between hfgl2expression and the severity of the liver disease as indicated by the levels of TBil. PCA significantly increased in PBMC in patients with severe AOC hepatitis B.CONCLUSION: The molecular and cellular results reported here in both mice and patients with severe viral hepatitis suggest that virus-induced hfgl2prothrombinase/fibroleukin expression and the coagulation activity associated with the encoded fgl2protein play a pivotal role in initiating severe hepatitis.The measurement of hfgl2/fibroleukin expression in PBMC may serve as a useful marker to monitor the severity of AOC hepatitis B and a target for therapeutic intervention. | Chuan-Long Zhu Wei-Ming Yan Fan Zhu Yong-Fen Zhu Dong Xi De-Ying Tian Gary Levy Xiao-Ping Luo Qin Ning | 2005 | World Journal of Gastroenterology2005，11，44： | 26 |
| 9 | Requirement and Functional Redundancy of Ib Subgroup bHLH Proteins for Iron Deficiency Responses and Uptake in Arabidopsis thaliana显示文摘在 Arabidopsis 的 bHLH 基因家庭的 Ib 亚群包含四个成员(AtbHLH38, AtbHLH39, AtbHLH100,和 AtbHLH101 ) 。AtbHLH38 和 AtbHLH39 以前被证实与象 FER 一样铁缺乏交往导致的抄写因素(合适) ,直接在 ferricchelate reductase FRO2 和高亲密关系的铁的铁 transporter IRT1 的表示的激活工作。在这个工作,我们在铁缺乏回答和举起的规定描绘了 AtbHLH100 和 AtbHLH101 的功能。酵母二混血儿的分析和双分子的荧光互补试金证明 AtbHLH100 和 AtbHLH101 能交往与合适。AtbHLH100 或 AtbHLH101 与的双表示装入激活的酵母房间 FRO2 和 IRT1 的倡导者驾驶的 GUS 表示。工厂 overexpressing 与 AtbHLH101 结合了在根显示出 FRO2 和 IRT1 的组成的表示,并且在射击积累了更多的铁。进一步, AtbHLH38, AtbHLH39, AtbHLH100,和 AtbHLH101 的单个、双、三倍的猛烈异种被产生并且描绘。在根的 FRO2 和 IRT1 表示和在射击的铁内容更急速地比双的可得到的其它和四基因的三倍的异种的在 AtbHLH39, AtbHLH100,和 AtbHLH101 的三倍的猛烈异种被减少。象在在铁缺乏下面的多重猛烈异种的 FRO2 和 IRT1 的表示一样的生理的回答的比较表明 AtbHLH100, AtbHLH38, AtbHLH101,和 AtbHLH39 在铁缺乏回答和举起起了逐渐地增加的重要作用。一起拿所有,我们断定四 Ib 亚群 bHLH 蛋白质在 Arabidopsis 为铁缺乏回答和举起的激活与微分意义被要求并且拥有冗余的功能。 | Ning Wang Yan Cui Yi Liu Huajie Fan Juan Du Zongan Huang Youxi Yuan Huilan Wu Hong-Qing Ling | 2013 | Molecular Plant2013，6，2： | 26 |
| 10 | Fibrinogen-like protein 2/fibroleukin prothrombinase contributes to tumor hypercoagulability via IL-2 and IFN-γ显示文摘AIM: To examine the role of Fibrinogen-like protein 2 (fgl2)/fibroleukin in tumor development. Fgl2 has been reported to play a vital role in the pathogenesis in MHV-3 (mouse hepatitis virus) induced fulminant and severe hepatitis,spontaneous abortion,allo-and xeno-graft rejection by mediating 'immune coagulation'. METHODS: Tumor tissues from 133 patients with six types of distinct cancers and the animal tumor tissues from human hepatocellular carcinoma (HCC) model on nude mice (established from high metastasis HCC cell line MHCC97LM6) were obtained. RESULTS: Hfgl2 was detected in tumor tissues from 127 out of 133 patients as well as tumor tissues collected from human HCC nude mice. Hfgl2 was highly expressed both in cancer cells and interstitial inflammatory cells including macrophages,NK cells,and CD8+ T lymphocytes and vascular endothelial cells. Hfgl2 mRNA was localized in cells that expressed hfgl2 protein. Fibrin (nogen) co-localization with hfgl2 expression was determined by dual immunohistochemical staining. In vitro,IL-2 and IFN-γ increased hfgl2 mRNA by 10-100 folds and protein expression in both THP-1 and HUVEC cell lines. One-stage clotting assays demonstrated that THP-1 and HUVEC cells expressing hfgl2 had increased procoagulant activity following cytokines stimulation. CONCLUSION: The hfg12 contributes to the hypercoagulability in cancer and may induce tumor angiogenesis and metastasis via cytokine induction. | Kai Su Fang Chen Wei-Ming Yan Qi-Li Zeng Li Xu Dong Xi Bin Pi Xiao-Ping Luo Qin Ning | 2008 | World Journal of Gastroenterology2008，14，39： | 20 |
| 11 | The ChinaMAP analytics of deep whole genome sequences in 10,588 individuals显示文摘Metabolic diseases are the most common and rapidly growing health issues worldwide.The massive population-based human genetics is crucial for the precise prevention and intervention of metabolic disorders.The China Metabolic Analytics Project(ChinaMAP)is based on cohort studies across diverse regions and ethnic groups with metabolic phenotypic data in China.Here,we describe the centralized analysis of the deep whole genome sequencing data and the genetic bases of metabolic traits in 10,588 individuals from the ChinaMAP.The frequency spectrum of variants,population structure,pathogenic variants and novel genomic characteristics were analyzed.The individual genetic evaluations of Mendelian diseases,nutrition and drug metabolism,and traits of blood glucose and BMI were integrated.Our study establishes a large-scale and deep resource for the genetics of East Asians and provides opportunities for novel genetic discoveries of metabolic characteristics and disorders. | Yanan Cao Lin Li Min Xu Zhimin Feng Xiaohui Sun Jieli Lu Yu Xu Peina Du Tiange Wang Ruying Hu Zhen Ye Lixin Shi Xulei Tang Li Yan Zhengnan Gao Gang Chen Yinfei Zhang Lulu Chen Guang Ning Yufang Bi Weiqing Wang The ChinaMAP Consortium | 2020 | Cell Research2020，30，9： | 20 |
| 12 | Effect of Snow-cover Duration on Plant Species Diversity of Alpine Meadows on the Eastern Qinghai-Tibetan Plateau显示文摘Although snow cover plays an important role in structuring plant diversity in the alpine zone,there are few studies on the relationship between snow cover and species diversity of alpine meadows on the eastern Qinghai-Tibetan Plateau.To assess the effect of snow cover on plant species diversity of alpine meadows,we used ten parallel transects of 60m×1m for this study and described the changes in species diversity and composition associated with snow depth.With the division of snow depth into six classes,the highest species richness(S)and species diversity(H')occurred with an intermediate snow depth,i.e.,class III and class IV,showing a unimodal curve with the increase in snow depth.The relationship between snow depth and plant diversity(both richness and Shannon index)could be depicted by quadratic equations.There was no evident relationship between diversity(both S and H')and soil water content,which implied that other more important factors influenced species diversity.The patterns of diversity found in our study were largely attributed to freeze-thaw alteration,length of growing season and disturbances of livestock grazing.Furthermore,snow depth affected species composition,as evaluated by the Srensen's index of similarity.In addition,almost all species limited to one snow depth class were found only in class III and class IV,indicating that intermediate snow depth was suitable for the survival and growth of many alpine species. | CHEN Wennian WU Yan WU Ning LUO Peng | 2008 | Journal of Mountain Science2008，5，4： | 19 |
| 13 | Liproxstatin-1 is an effective inhibitor of oligodendrocyte ferroptosis induced by inhibition of glutathione peroxidase 4显示文摘Our previous studies showed that ferroptosis plays an important role in the acute and subacute stages of spinal cord injury.High intracellular iron levels and low glutathione levels make oligodendrocytes vulnerable to cell death after central nervous system trauma.In this study,we established an oligodendrocyte(OLN-93 cell line)model of ferroptosis induced by RSL-3,an inhibitor of glutathione peroxidase 4(GPX4).RSL-3 significantly increased intracellular concentrations of reactive oxygen species and malondialdehyde.RSL-3 also inhibited the main antiferroptosis pathway,i.e.,SLC7A11/glutathione/glutathione peroxidase 4(xCT/GSH/GPX4),and downregulated acyl-coenzyme A synthetase long chain family member 4.Furthermore,we evaluated the ability of several compounds to rescue oligodendrocytes from ferroptosis.Liproxstatin-1 was more potent than edaravone or deferoxamine.Liproxstatin-1 not only inhibited mitochondrial lipid peroxidation,but also restored the expression of GSH,GPX4 and ferroptosis suppressor protein 1.These findings suggest that GPX4 inhibition induces ferroptosis in oligodendrocytes,and that liproxstatin-1 is a potent inhibitor of ferroptosis.Therefore,liproxstatin-1 may be a promising drug for the treatment of central nervous system diseases. | Bao-You Fan Yi-Lin Pang Wen-Xiang Li Chen-Xi Zhao Yan Zhang Xu Wang Guang-Zhi Ning Xiao-Hong Kong Chang Liu Xue Yao Shi-Qing Feng | 2021 | Neural Regeneration Research2021，16，3： | 19 |
| 14 | Circumspinal decompression through a single posterior incision to treat thoracolumbar disc herniation显示文摘背景各种各样的外科的途径成功地在 thoracolumbar 圆盘形成疝( TLDH )的处理被使用了前面的 transthoracic 途径有的 .Although 为更好的可视化的名声比 posterolateral 和侧面的途径,它包含胸、肺的 structures.Thus 的操作,这条途径对损害呼吸 system.An 理想途径技术上费力、容易将包含足够的可视化并且通过 pos 被完成意味着年龄 51 年)通过在2008年1月和2009年12月 .Altogether 之间的一个单个以后的切口过程与征兆的 TLDH 经历了 circumspinal 解压缩, 17 个 herniated 磁盘被切除,与在在在 T12/L1 的 T11/12,5 磁盘的 T10/11,4 磁盘的 2 个磁盘和在 L1/2.Of 的 6 个磁盘这些病人, 13 被跟随在上面与 23.5 months.Clinical 结果的一个吝啬的后续时期包括起作用的时间,血损失, perioperative 复杂并发症,住院的手术后的时间,神经地位 | QI Qiang CHEN Zhong-qiang LIU Ning GUO Zhao-qing SHI Ze-feng LIU Zhong-jun LIU Xiao-guang LI Wei-shi ZENG Yan SUN Chui-guo | 2011 | Chinese Medical Journal2011，，23： | 17 |
| 15 | Establishment of hepatocellular carcinoma multidrug resistant monoclone cell line HepG2/mdr1显示文摘multidrug 抵抗(MDR ) 与 mdr1 基因和它的产品 P-glycoprotein 的表示联系了的背景是在癌房间(HCC ) 病人与化疗对待的 hepatocellular 的预后的一个主要因素。我们的学习是建立明确地,与转基因的 mdr1.Methods 的 overexpression 有关, 4.5-kb mdr1 cDNA 从 plasmid pHaMDR1-1 获得了的 multidrug 抵抗的 de novo 获得被克隆进 PCI-neo 哺乳动物的表示向量的一根稳定的 HCC MDR 房间线,后来被 liposome 转移到人的 hepatocarcinoma 房间线 HepG2 。然后,抵抗 G418 的 transfected HepG2 房间被聚类并且有教养并且在这些 HepG2 房间的 mdr1 cDNA, mRNA 和 P-glycoprotein (Pgp ) 的特定的碎片被 PCR, RT-PCR 和流动 cytometry 分别地检测。daunorubicin 的累积同时是由流动 cytometry 的 determinated。grafting 瘤的裸体老鼠模特儿被皮下地在正确腋下注射 HepG2/mdr1 房间建立。当瘤直径到达了 5 公里时, adriamycin 被注入腹洞。瘤的尺寸和生长抑制是 evaluated.Results mdr1 表示向量成功地被构造, MDR HCC 线 HepG2/mdr1 developed.The PCR 分析证明 mdr1 的特定的碎片在 HepG2/mdr1 的 cDNA 房间,然而并非在控制组 HepG2 房间。而且,在 HepG2/mdr1 房间的 mdr1 mRNA 和 Pgp 表示的特定的碎片的内容是(59. | CHEN Yong-bing YAN Mao-lin GONG Jian-ping XIA Ren-pin LIU Li-xin LI Ning LU Shi-chun ZHANG Jing-guang ZENG Dao-bing XIE Jian-guo YANG Jia-yin YAN Lü-nan | 2007 | Chinese Medical Journal2007，，8： | 16 |
| 16 | COVID-19 Outbreak Caused by Contaminated Packaging of Imported Cold-Chain Products—Liaoning Province,China,July 2020显示文摘Summary What is known about this topic?Few major outbreaks of coronavirus disease 2019(COVID-19)have occurred in China after major nonpharmaceutical interventions and vaccines have been deployed and implemented.However,sporadic outbreaks that had high possibility to be linked to cold chain products were reported in several cities of China..What is added by this report?In July 2020,a COVID-19 outbreak occurred in Dalian,China.The investigations of this outbreak strongly suggested that the infection source was from COVID-19 virus-contaminated packaging of frozen seafood during inbound unloading personnel contact.What are the implications for public health practice?Virus contaminated paper surfaces could maintain infectivity for at least 17–24 days at-25℃.Exposure to COVID-19 virus-contaminated surfaces is a potential route for introducing the virus to a susceptible population.Countries with no domestic transmission of COVID-19 should consider introducing prevention strategies for both inbound travellers and imported goods.Several measures to prevent the introduction of the virus via cold-chain goods can be implemented. | Huilai Ma1 Jianqun Zhang Ji Wang Ying Qin Cao Chen Yang Song Liang Wang Jun Meng Lingling Mao Fengqin Li Ning Li Jian Cai Yong Zhang Dayan Wang Yunting Xia Hong Wang Shaofeng Jiang Xiang Zhao Peihua Niu Wenjie Tan Tao Ma Yecheng Yao Naiying Mao Zhen Zhu Tianjiao Ji Qian Yang Baoying Huang Li Zhao Jianxing Yu Li Bai Shuangli Zhu Dongyan Wang Yan Zhang Yingwei Sun Mingchun Luan Yanhai Wang Haibo Sun Shihong Yang Zhijian Bo Xiang Ren Zhongjie Li George Fu Gao Wei Yao Wenqing Yao Zijian Feng Wenbo Xu | 2021 | China CDC weekly2021，3，21： | 16 |
| 17 | Effects of cryopreservation and phenylacetate on biological characters of adherent LAK cells from patients with hepatocellular carcinoma显示文摘AIM: To improve the preparation of adherent lymphokine-activated killer (A-LAK) cells and to study the effects ofcryopreservation and phenylacetate (PA) on biologicalcharacters of A-LAK cells.METHODS: A-LAK cells were obtained from peripheral bloodmononuclear cells (PBMCs) of the patients withhepatocellular caminoma (HCC) by using L-phenylalaninemethyl ester ( PME ) to deplete immunosuppressivermonocytes. Proliferative activity of SMMC7721 cell line aftertreatment with phenylacetate (PA) was observed. A-LAKcells were treated with the supernatant of SMMC7721 cellsthat had been pretreated with PA. The changes ofproliferation, cytotoxicity and phenotype of A-LAK cellswere investigated after cryopreservstion.RESULTS: The expansion of A-LAK cells (96.79± 69.10 foldson Day 14 ) was significantly higher then that of non-adherent LAK (NA-LAK) cells (22.77 ± 13.20) as well asconventional LAK cells (4. 64 ± 0. 91 ). PA significantlysuppressed the growth of SMMC7721 cells, and the inhibitorratio was 46 %. rite supernatant of cultured tumor cellsintensively suppressed the proliferation and cytotoxicity ofA-LAK cells, but the suppressive effect of the supernatantwas previously decreased after treatment with PA.Impairments in proliferation and cytotoxicity of A-LAK cellsimmediately after thawing of cryopreservation and recoveryafter reincubation with IL-2 were observed. The cytotoxicltyof thawed A-LAK cells on Day 5 was significantly higher thanthat of fresh A-LAK before freezing (54.8 ± 10.2 % vs 40.5 ±6. 4 %). No significant change in the percentage oflymphocyte subsets was identified in frozen A-LAK cells ascompared with that in the fresh control cells.CONCLUSION: A-LAK cells can he simply prepared by usingPME, and showed a synergistic anti-tumor effect with thecombination of PA. Cryopreseration can increase theimmunoactivities of A-LAK cells from the patients withhepatocellular caminoma . | Ning Zheng Sheng-Long Ye Rui-Xia Sun Yan Zhao Zhao-You Tang,Liver Cancer Institute and Zhongshan Hospital,Fudan University,Shanghai 200032,China | 2002 | World Journal of Gastroenterology2002，8，2： | 16 |
| 18 | Emodin alleviates intestinal mucosal injury in rats with severe acute pancreatitis via the caspase-1 inhibition显示文摘BACKGROUND:Emodin,a traditional Chinese medicine,has a therapeutic effect on severe acute pancreatitis(SAP),whereas the underlying mechanism is still unclear.Studies showed that the intestinal mucosa impairment,and subsequent release of endotoxin and proinflammatory cytokines such as IL-1β,which further leads to the dysfunction of multiple organs,is the potentially lethal mechanism of SAP.Caspase-1,an IL-1β-converting enzyme,plays an important role in this cytokine cascade process.Investigation of the effect of emodin on regulating the caspase-1 expression and the release proinflammatory cytokines will help to reveal mechanism of emodin in treating SAP.METHODS:Eighty Sprague-Dawley rats were randomly divided into four groups(n=20 each group):SAP,sham-operated(SO),emodin-treated(EM) and caspase-1 inhibitor-treated(ICE-I) groups.SAP was induced by retrograde infusion of 3.5% sodium taurocholate into the pancreatic duct.Emodin and caspase-1 inhibitor were given 30 minutes before and 12 hours after SAP induction.Serum levels of IL-1β,IL-18 and endotoxin,histopathological alteration of pancreas tissues,intestinal mucosa,and the intestinal caspase-1 m RNA and protein expressions were assessed 24 hours after SAP induction.RESULTS:Rats in the SAP group had higher serum levels of IL-1β and IL-18(P<0.05),pancreatic and gut pathological scores(P<0.05),and caspase-1 m RNA and protein expressions(P<0.05) compared with the SO group.Compared with the SAP group,rats in the EM and ICE-I groups had lower IL-1β and IL-18 levels(P<0.05),lower pancreatic and gut pathological scores(P<0.05),and decreased expression of intestine caspase-1 m RNA(P<0.05).Ultrastructural analysis by transmission electron microscopy found that rats in the SAP group had vaguer epithelial junctions,more disappeared intercellular joints,and more damaged intracellular organelles compared with those in the SO group or the EM and ICE-I groups.CONCLUSIONS:Emodin alleviated pancreatic and intestinal mucosa injury in experimental SAP.Its mechanism may partly be mediated by the inhibition of caspase-1 and its downstream inflammatory cytokines,including IL-1β and IL-18.Our animal data may be applicable in clinical practice. | Jian-Wen Ning Yan Zhang Mo-Sang Yu Meng-Li Gu Jia Xu Ali Usman Feng Ji | 2017 | Hepatobiliary & Pancreatic Diseases International2017，16，4： | 15 |
| 19 | Do pyroptosis, apoptosis, and necroptosis (PANoptosis) exist in cerebral ischemia? Evidence from cell and rodent studies显示文摘Some scholars have recently developed the concept of PANoptosis in the study of infectious diseases where pyroptosis,apoptosis and necroptosis act in consort in a multimeric protein complex,PANoptosome.This allows all the components of PANoptosis to be regulated simultaneously.PANoptosis provides a new way to study the regulation of cell death,in that different types of cell death may be regulated at the same time.To test whether PANoptosis exists in diseases other than infectious diseases,we chose cerebral ischemia/reperfusion injury as the research model,collected articles researching cerebral ischemia/reperfusion from three major databases,obtained the original research data from these articles by bibliometrics,data mining and other methods,then integrated and analyzed these data.We selected papers that investigated at least two of the components of PANoptosis to check its occurrence in ischemia/reperfusion.In the cell model simulating ischemic brain injury,pyroptosis,apoptosis and necroptosis occur together and this phenomenon exists widely in different passage cell lines or primary neurons.Pyroptosis,apoptosis and necroptosis also occurred in rat and mouse models of ischemia/reperfusion injury.This confirms that PANoptosis is observed in ischemic brain injury and indicates that PANoptosis can be a target in the regulation of various central nervous system diseases. | Wei-Tao Yan Yan-Di Yang Xi-Min Hu Wen-Ya Ning Lyu-Shuang Liao Shuang Lu Wen-Juan Zhao Qi Zhang Kun Xiong | 2022 | Neural Regeneration Research2022，17，8： | 15 |
| 20 | MiR-181b suppresses proliferation of and reduces chemoresistance to temozolomide in U87 glioma stem cells显示文摘MicroRNAs regulate self renewal and differentiation of cancer stem cells.There,we sought to identify the expression of miR-181b in glioma stem cells and investigate the biological effect of miR-181b on glioma stem cells in this study.MiR-181b expression was measured by real-time PCR in glioma stem cells isolated from U87 cells by FACS sorting.After miR-181b was overexpressed in U87 glioma stem cells by miR-181b lentiviral expression vector and/or treatment of temozolomide,secondary neurosphere assay,soft agar colony assay and MTT assay were performed.Compared with U87 cells,the expression of miR-181b was significantly decreased in U87 glioma stem cells.Overexpression of miR-181b decreased neurosphere formation by U87 glioma stem cells in vitro and suppressed colony formation in soft agar,and the cell growth inhibition rates increased in a time-dependent manner in U87 glioma stem cells infected with miR-181b lentivirus.Furthermore,miR-181b had a synergistic effect on temozolomide-induced inhibition of secondary neurosphere and soft agar colony,and on cell growth inhibition rates.MiR-181b functions as a tumor suppressor that suppresses proliferation and reduces chemoresistance to temozolomide in glioma stem cells. | Ping Li Xiaoming Lu Yingyi Wang Lihua Sun Chunfa Qian Wei Yan Ning Liu Yongping You Zhen Fu | 2010 | The Journal of Biomedical Research2010，24，6： | 14 |
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