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| 1 | Flow cytometric detection of hepatitis C virus antigens in infected peripheral blood leukocytes: Binding and entry显示文摘AIM: We designed two synthetic-core-specific peptides core 1 (C1) and core 2 (C2), and an E1-specific peptide (E1). We produced specific polyclonal antibodies againstthese peptides and used the antibodies for detection of HCV antigens on surface and within infected peripheral blood leukocytes.METHODS: Peripheral blood from a healthy individual who tested negative for HCV RNA was incubated with HCV type 4 infected serum for 1 h and 24 h at 37 ℃. Cells were stained by direct and indirect immunofluorescence and measured by flow cytometry.RESULTS: After 1 h of incubation, antibodies against C1,C2, and E1 detected HCV antigens on the surface of 27%,26% and 73% of monocytes respectively, while 10%, 5% and 9% of lymphocytes were positive with anti-C1, anti-C2 and anti-E1 respectively. Only 1-3% of granulocytes showed positive staining with anti-C1, anti-C2 and anti E1 antibodies. After 24 h of incubation, we found no surface staining with anti-C1, anti-C2 or anti-E1. Direct immunostaining using anti-C2 could not detect intracellular HCV antigens, after 1 h of incubation with the virus, while after 24 h of incubation, 28% of infected cells showed positive staining. Only plus strand RNA was detectable intracellularly as early as 1 h after incubation, and remained detectable throughout 48 h post-infection.Interestingly, minus RNA strand could not be detected after 1 h, but became strongly detectable intracellularly after 24 h post-infection.CONCLUSION: Monocytes and lymphocytes are the preferred target cells for HCV infection in peripheral blood leukocytes. Our specific anti-core and anti-E1 antibodies are valuable reagents for demonstration of HCV cell cycle.Also, HCV is capable of infecting and replicating in peripheral blood mononuclear cells as confirmed by detection of minus strand HCV RNA as well as intracellular staining of core HCV antigen. | Mostafa K El-Awady Ashraf A Tabll El-Rashdy M Redwan Samar Youssef Moataza H Omran Fouad Thakeb Maha El-Demellawy | 2005 | World Journal of Gastroenterology2005，11，33： | 4 |
| 2 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 3 | The pharmacokinetics of the prostaglandin E1 analogue misoprostol in plasma and colostrum after postpartum oral administration显示文摘 | Hany Abdel-Aleem José Villar A Metin Gülmezoglu Sayed A Mostafa Alaa A Youssef Mahmoud Shokry Bernhard Watzer | 2002 | European Journal of Obstetrics and Gynecology2002，，1： | 2 |
| 4 | 在有煽动性、非煽动性的腹的疼痛显型的孩子的 interleukin-6 和桅杆房间的反的关系显示文摘 AIM: To investigate interleukin-6 (IL-6), mast cells, enterochromaffin cells, 5-hydroxytryptamine, and substance P in the gastrointestinal mucosa of children with abdominal pain. METHODS: Formalin-fixed paraffin-embedded gas-trointestinal biopsy blocks from patients (n = 48) with non-inflammatory bowel disease (irritable bowel syndrome and functional abdominal pain) and inflammatory bowel disease were sectioned and stained for IL-6, mast cells, enterochromaffin cells, 5-hydroxytryptamine, and substance P. All children had chronic abdominal pain as part of their presenting symptoms. Biopsy phenotype was confirmed by a pathologist, blinded to patient information. Descriptive statistics, chi-square, and independent sample t tests were used to compare differences between the inflammatory and non-inflammatory groups. RESULTS: The cohort (n = 48), mean age 11.9 years (SD = 2.9), 54.2% females, 90% Caucasian, was comprised of a non-inflammatory (n = 26) and an inflammatory (n = 22) phenotype. There was a significant negative correlation between substance P expression and mast cell count (P = 0.05, r = -0.373). Substance P was found to be expressed more often in female patient biopsies and more intensely in the upper gastrointestinal mucosa as compared to the lower mucosa. There were significantly increased gastrointestinal mucosal immunoreactivity to IL-6 (P = 0.004) in the inflammatory phenotype compared to non-inflammatory. Additionally, we found significantly increased mast cells (P = 0.049) in the mucosa of the non-inflammatory phenotype compared to the inflammatory group. This difference was particularly noted in the lower colon biopsies. CONCLUSION: The findings of this study yield preliminary evidence in identifying biomarkers of undiagnosed abdominal pain in children and may suggest candidate genes for future evaluation. | Wendy A Henderson Ravi Shankar Tara J Taylor Arseima Y Del Valle-Pinero David E Kleiner Kevin H Kim Nader N Youssef | 2012 | World Journal of Gastrointestinal Pathophysiology2012，3，6： | 2 |
| 5 | Early detection of hepatocellular carcinoma co-occurring with hepatitis C virus infection: A mathematical model显示文摘AIM: To develop a mathematical model for the early detection of hepatocellular carcinoma(HCC) with a panel of serum proteins in combination with α-fetoprotein(AFP).METHODS: Serum levels of interleukin(IL)-8, soluble intercellular adhesion molecule-1(s ICAM-1), soluble tumor necrosis factor receptor Ⅱ(s TNF-RⅡ), proteasome, and β-catenin were measured in 479 subjects categorized into four groups:(1) HCC concurrent with hepatitis C virus(HCV) infection(n = 192);(2) HCV related liver cirrhosis(LC)(n = 96);(3) Chronic hepatitis C(CHC)(n = 96); and(4) Healthy controls(n = 95). The R package and different modules for binary and multi-class classifiers based on generalized linear models were used to model the data. Predictive power was used to evaluate the performance of the model. Receiver operating characteristic curve analysis over pairs of groups was used to identify the best cutoffs differentiating the different groups. RESULTS: We revealed mathematical models, based on a binary classifier, made up of a unique panel of serum proteins that improved the individual performance of AFP in discriminating HCC patients from patients with chronic liver disease either with or without cirrhosis. We discriminated the HCC group from the cirrhotic liver group using a mathematical model(-11.3 + 7.38 × Prot + 0.00108 × s ICAM + 0.2574 ×β-catenin + 0.01597 × AFP) with a cutoff of 0.6552, which achieved 98.8% specificity and 89.1% sensitivity. For the discrimination of the HCC group from the CHC group, we used a mathematical model [-10.40 + 1.416 × proteasome + 0.002024 × IL + 0.004096 × s ICAM-1 +(4.251 × 10-4) × s TNF + 0.02567 ×β-catenin + 0.02442 × AFP] with a cutoff 0.744 and achieved 96.8% specificity and 89.7% sensitivity. Additionally, we derived an algorithm, based on a binary classifier, for resolving the multi-class classification problem by using three successive mathematical model predictions of liver disease status. CONCLUSION: Our proposed mathematical model may be a useful method for the early detection of different statuses of liver disease co-occurring with HCV infection. | Abdel-Rahman Nabawy Zekri Amira Salah El-Din Youssef Yasser Mabrouk Bakr Reham Mohamed Gabr Ola Sayed Ahmed Mostafa Hamed Elberry Ahmed Mahmoud Mayla Mohamed Abouelhoda Abeer A Bahnassy | 2016 | World Journal of Gastroenterology2016，22，16： | 2 |
| 6 | Radiolarian and Planktic Foraminifera Biostratigraphy of the Early Albian Organic Rich Beds of Fahdene Formation,Northern Tunisia显示文摘The lower part of Fahdene Formation outcropping in northeastern Tunisia is represented by pelagic sequences and characterized by an important siliciclastic fraction that includes radiolarian and organic-rich beds of Allam Member.?Litho-biostratigraphic analysis based on planktic foraminifera and radiolaria shows that deposition of organic-rich beds of Allam Member is confined to the Microhedbergella rischi Zone through the lower part of Ticinella primula Zone.?Age diagnostic radiolaria recovered from these beds has been identified to constrain a direct age of black shale deposition.The assemblages can be correlated with the radiolarian biochronozone U.A.10–11 indicating an Early Albian to early Middle Albian in age.Radiolarian assemblages composed of species characteristic of U.A.10 biochronozone(A.montisserei,D.gracilis)are associated with radiolarian taxa belonging to Costata Zone(U.A.9 Pseudoeucyrtis hanni and Thanarla pseudodecora),which can be correlated with Dercourt Member in the Ionian Zone and Paquier level of the Vocontian Basin(southeast of France).Biostratigraphic and abundance curves analysis has demonstrated significant diversification of cryptocephalic Nassellaria and Archaeodictyomitrae,probably adapted to eutrophic conditions that characterized the Aptian-Albian transition.Ecological conditions may have governed the stratigraphic range of many cosmopolitan taxa(i.e.,Pseudodictyomitrae lodogaensis)compared with stratigraphic distributions schemes reported from other domains.The timing of black shales deposition was diachronic due to local geodynamic conditions and upwelling currents distribution.The Allam black shales are correlative with the oceanic anoxic event OAE1b which is characterized by the widespread of supraregionnally organic-rich beds in the Mediterranean Tethys basins across the Aptian-Albian transition. | M Ben Fadhel T Zouaghi A Amri M Ben Youssef | 2014 | Journal of Earth Science2014，25，1： | 2 |
| 7 | Affine equivalence in the AES round function显示文摘 | Youssef A M Tavares S E | 2005 | Discrete Applied Mathematics2005，148，2： | 1 |
| 8 | Modelling and opti mization of multiple-aspect RMS configurations显示文摘 | YOUSSEF A M A ELMARAGHY H A | 2006 | Interna tional Journal of Production Research2006，44，22： | 1 |
| 9 | Surface modification of Ti-6Al-4V alloy by nitrogen ion implantation 显示文摘 | Budzynski P Youssef A A Sielanko J | 2006 | Wear2006，261，： | 1 |
| 10 | Musa genetic diversityrevealed by SRAP and AFLP显示文摘 | Youssef M James A C Rivera M R | 2011 | Molecular Biotechnology2011，47，3： | 1 |
| 11 | Curcumin and its carboeyelie analogs: structure-activity in relation to antioxidant and selected biological properties显示文摘 | BHULLAR S JHA A YOUSSEF D | 2013 | Molecules2013，18，5： | 1 |
| 12 | Hashash,Nonlinear Ground-Response Analysis of Turkey Flat Shallow Stiff-soil Site to Strong Ground Motion显示文摘 | Annie 0 L Kwok Jonathan P Stewart Youssef M A | 2008 | Bulletin ot' the Seis- mological Society of America February2008，98，： | 1 |
| 13 | Effect of simultane-ous down-regulation of pectate lyase and endo-(3-l,4-glucanasegenes on strawberry fruit softening显示文摘 | YOUSSEF S M IRAIDA A L^PEZ-ARANDA J M’ RAFAELS VICTORIANO V GIORGIO C ROSARIO B-P FERNAN-DO P-A OUESADA M A MERCADO J A | 2013 | Molecular Breeding2013，31，： | 1 |
| 14 | Haemodynamic alterations induced by toxic level of sodium taurocholate 显示文摘 | Youssef SA Ramadan A Afifi NA | 1991 | Dtsch Tierarztl Wochenschr1991，98，2： | 1 |
| 15 | Optimal configura tion selection for reconfigurable manufacturing systems显示文摘 | YOUSSEF A M A ELMARAGHY H A | 2007 | International Journal of Flexible Manufacturing Systems2007，19，2： | 1 |
| 16 | Epidemiological aspects ofmango malformation disease caused by Fusarium mangiferae andsource of infection in seedlings cultivated in orchards in Egypt显示文摘 | YOUSSEF S A MAYMONB M ZVEIBIL A GUETA D K SZTEJN-BERG A SHALABY A FREEMAN S | 2007 | Plant Pathology2007，56，： | 1 |
| 17 | Paliperidone extendedrelease tablet s in schizophrenia patients previously treated with risperidone 显示文摘 | CANUSO C M YOUSSEF E A BOSSIE C A | 2008 | Int Clin Psychopharmacol2008，23，4： | 1 |
| 18 | Realtime speckle reduction and coherence enhancement in ultrasound imaging via nonlinear anisotropic diffusion显示文摘 | KHALED Z A YOUSSEF M K KADAH Y M | 2002 | IEEE Transactions on Biomedical Engineering2002，49，9： | 1 |
| 19 | Stress- strain model for concrete confined by FRP composites 显示文摘 | Youssef M N Feng M Q Mosallam A S | 2007 | Composites: Part B2007，38，56： | 1 |
| 20 | Musa genetic diversity revealed by SRAP and AFLP 显示文摘 | Youssef M James A C Rivera-Madrid R | 2011 | Molecular Bio- technology2011，47，3： | 1 |
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