|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Anti-SARS-CoV-2 activities in vitro of Shuanghuanglian preparations and bioactive ingredients显示文摘Human infection with severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)causes coronavirus disease 2019(COVID-19)and there is no cure currently.The 3CL protease(3CLpro)is a highly conserved protease which is indispensable for CoVs replication,and is a promising target for development of broad-spectrum antiviral drugs.In this study we investigated the anti-SARS-CoV-2 potential of Shuanghuanglian preparation,a Chinese traditional patent medicine with a long history for treating respiratory tract infection in China.We showed that either the oral liquid of Shuanghuanglian,the lyophilized powder of Shuanghuanglian for injection or their bioactive components dose-dependently inhibited SARS-CoV-23CLpro as well as the replication of SARS-CoV-2 in Vero E6 cells.Baicalin and baicalein,two ingredients of Shuanghuanglian,were characterized as the first noncovalent,nonpeptidomimetic inhibitors of SARS-CoV-23CLpro and exhibited potent antiviral activities in a cell-based system.Remarkably,the binding mode of baicalein with SARS-CoV-23CLpro determined by X-ray protein crystallography was distinctly different from those of known 3CLpro inhibitors.Baicalein was productively ensconced in the core of the substrate-binding pocket by interacting with two catalytic residues,the crucial S1/S2 subsites and the oxyanion loop,acting as a“shield”in front of the catalytic dyad to effectively prevent substrate access to the catalytic dyad within the active site.Overall,this study provides an example for exploring the in vitro potency of Chinese traditional patent medicines and effectively identifying bioactive ingredients toward a specific target,and gains evidence supporting the in vivo studies of Shuanghuanglian oral liquid as well as two natural products for COVID-19 treatment. | Hai-xia Su Sheng Yao Wen-feng Zhao Min-jun Li Jia Liu Wei-juan Shang Hang Xie Chang-qiang Ke Hang-chen Hu Mei-na Gao Kun-qian Yu Hong Liu Jing-shan Shen Wei Tang Lei-ke Zhang Geng-fu Xiao Li Ni Dao-wen Wang Jian-ping Zuo Hua-liang Jiang Fang Bai Yan Wu Yang Ye Ye-chun Xu | 2020 | Acta Pharmacologica Sinica2020，41，9： | 32 |
| 2 | Identification of a natural mutant of HBV X protein truncated 27 amino acids at the COOH terminal and its effect on liver cell proliferation显示文摘目的:识别肝炎 B ( HBV ) X ( HBx )基因的异种并且在肝房间 proliferation.Methods 上调查自然异种的效果:我们从 188 重量的单位识别了 HBx 基因的自然异种一中国病人的 48 纸巾由 PCR 与 HBV 感染了的and , HBx 基因的异种的鉴定上的 respectively.Based ,我们克隆异种的碎片进 pcDNA3 向量。异种的生物活动是 investigated.Results : 我们从 3 重量的单位从 382 ~ 401 基础对与删除识别了 HBx 基因的自然异种一 out of 188 个病人,它在 COOH 终端从第 128 氨基酸导致了 HBx 蛋白质的表达式删除。有从在 COOH 终端的 382 基础对的删除的类似的异种从 48 肝细胞癌纸巾从染色体的 5 个案例被识别。关于异种的生物活动,我们发现短暂 transfection 没有通过到 induce apoptosis,但是支持了人的肝的增长的 HBx 蛋白质的异种由稳定的 transfection 使 L-02 房间不朽,与野类型的 HBx 蛋白质相比。数据证明变异的 stably-trans-fected L-02-X-Sera 房间和碎片 stably-transfected L-02-XA127 房间的增长被 BrdU 加入试金提高,流动血细胞计数 analysis.Lu-ciferase 记者基因试金证明颠倒 transcriptase 是起来的 NF-KB , survivin ,和人的 telomerase 的 transcriptional 活动调整了,并且西方的污点分析表明原子抗原( PCNA )是起来的 c-Myc 和增殖的房间的表达式层次在 cells.Conclusion 调整了:我们的调查结果建议自然 HBx 异种在 COOH 终端截断了 27 氨基酸支持房间增长。 | Hang ZHANG Chang-liang SHAN Nan LI Xuan ZHANG Xue-zhi ZHANG Fu-qing XU Shuai ZHANG Li-yan QIU Li-hong YE Xiao-dong ZHANG | 2008 | Acta Pharmacologica Sinica2008，29，4： | 28 |
| 3 | The second natural gas hydrate production test in the South China Sea显示文摘Clayey silt reservoirs bearing natural gas hydrates(NGH)are considered to be the hydrate-bearing reservoirs that boast the highest reserves but tend to be the most difficult to exploit.They are proved to be exploitable by the first NGH production test conducted in the South China Sea in 2017.Based on the understanding of the first production test,the China Geological Survey determined the optimal target NGH reservoirs for production test and conducted a detailed assessment,numerical and experimental simulation,and onshore testing of the reservoirs.After that,it conducted the second offshore NGH production test in 1225 m deep Shenhu Area,South China Sea(also referred to as the second production test)from October 2019 to April 2020.During the second production test,a series of technical challenges of drilling horizontal wells in shallow soft strata in deep sea were met,including wellhead stability,directional drilling of a horizontal well,reservoir stimulation and sand control,and accurate depressurization.As a result,30 days of continuous gas production was achieved,with a cumulative gas production of 86.14×104 m3.Thus,the average daily gas production is 2.87×10^4 m^3,which is 5.57 times as much as that obtained in the first production test.Therefore,both the cumulative gas production and the daily gas production were highly improved compared to the first production test.As indicated by the monitoring results of the second production test,there was no anomaly in methane content in the seafloor,seawater,and atmosphere throughout the whole production test.This successful production test further indicates that safe and effective NGH exploitation is feasible in clayey silt NGH reservoirs.The industrialization of hydrates consists of five stages in general,namely theoretical research and simulation experiments,exploratory production test,experimental production test,productive production test,and commercial production.The second production test serves as an important step from the exploratory production test to experimental production test. | Jian-liang Ye Xu-wen Qin Wen-wei Xie Hai-long Lu Bao-jin Ma Hai-jun Qiu Jin-qiang Liang Jing-an Lu Zeng-gui Kuang Cheng Lu Qian-yong Liang Shi-peng Wei Yan-jiang Yu Chunsheng Liu Bin Li Kai-xiang Shen Hao-xian Shi Qiu-ping Lu Jing Li Bei-bei Kou Gang Song Bo Li He-en Zhang Hong-feng Lu Chao Ma Yi-fei Dong Hang Bian | 2020 | China Geology2020，3，2： | 22 |
| 4 | Isolation and characterization of dengue virus serotype 2 from the large dengue outbreak in Guangdong, China in 2014显示文摘Dengue has been well recognized as a global public health threat,but only sporadic epidemics and imported cases were reported in recent decades in China.Since July 2014,an unexpected large dengue outbreak has occurred in Guangdong province,China,resulting in more than 40000 patients including six deaths.To clarify and characterize the causative agent of this outbreak,the acute phase serum from a patient diagnosed with severe dengue was subjected to virus isolation and high-throughput sequencing(HTS).Traditional real-time RT-PCR and HTS with Ion Torrent PGM detected the presence of dengue virus serotype 2(DENV-2).A clinical DENV-2 isolate GZ05/2014 was obtained by culturing the patient serum in mosquito C6/36 cells.The complete genome of GZ05/2014 was determined and deposited in Gen Bank under the access number KP012546.Phylogenetic analysis based on the complete envelope gene showed that the newly DENV-2 isolate belonged to Cosmopolitan genotype and clustered closely with other Guangdong strains isolated in the past decade.No amino acid mutations that are obviously known to increase virulence or replication were identified throughout the genome of GZ05/2014.The high homology of Guangdong DENV-2 strains indicated the possibility of establishment of local DENV-2 circulation in Guangdong,China.These results help clarify the origin of this epidemic and predict the future status of dengue in China. | ZHAO Hui ZHAO Ling Zhai JIANG Tao LI Xiao Feng FAN Hang HONG Wen Xin ZHANG Yu ZHU Qin YE Qing TONG Yi Gang CAO Wu Chun ZHANG Fu Chun QIN Cheng Feng | 2014 | Science China(Life Sciences)2014，57，12： | 21 |
| 5 | The HuangZaoSi Maize Genome Provides Insights into Genomic Variation and Improvement History of Maize显示文摘Maize is a globally important crop that was a classic model plant for genetic studies. Here, we report a 2.2 Gb draft genome sequence of an elite maize line, HuangZaoSi (HZS). Hybrids bred from HZS-improved lines (HILs) are planted in more than 60% of maize fields in China. Proteome clustering of six completed sequeneed maize genomes show that 638 proteins fall into 264 HZS-specific gene families with the majority of contributions from tandem duplication events. Resequencing and comparative analysis of 40 HZSrelated lines reveals the breeding history of HILs. More than 60% of identified selective sweeps were clustered in identity.by.descent conserved regions, and yield-related genes/QTLs were enriched in HZS characteristic selected regions. Furthermore, we dem on strated that HZS-specific family genes were not uniformly distributed in the genome but enriched in improvement/function.related genomic regions. This study provides an important and novel resource for maize genome research and expands our knowledge on the breadth of genomic variation and improvement history of maize. | Chunhui Li Wei Song Yingfeng Luo Shenghan Gao Ruyang Zhang Zi Shi Xiaqing Wang Ronghuan Wang Fengge Wang Jidong Wang Yanxin Zhao Aiguo Su Shuai Wang Xin Li Meijie Luo Shuaishuai Wang Yunxia Zhang Jianrong Ge Xinyu Tan Ye Yuan Xiaochun Bi Hang He Jianbing Yan Yuandong Wang Songnian Hu Jiuran Zhao | 2019 | Molecular Plant2019，12，3： | 15 |
| 6 | High-security-level multi-dimensional optical storage medium:nanostructured glass embedded with LiGa_(5)O_(8):Mn^(2+) with photostimulated luminescence显示文摘The launch of the big data era puts forward challenges for information preservation technology,both in storage capacity and security.Herein,a brand new optical storage medium,transparent glass ceramic(TGC)embedded with photostimulated LiGa5O8:Mn2+nanocrystals,capable of achieving bit-by-bit optical data write-in and read-out in a photon trapping/detrapping mode,is developed.The highly ordered nanostructure enables light–matter interaction with high encoding/decoding resolution and low bit error rate.Importantly,going beyond traditional 2D optical storage,the high transparency of the studied bulk medium makes 3D volumetric optical data storage(ODS)possible,which brings about the merits of expanded storage capacity and improved information security.Demonstration application confirmed the erasable-rewritable 3D storage of binary data and display items in TGC with intensity/wavelength multiplexing.The present work highlights a great leap in photostimulated material for ODS application and hopefully stimulates the development of new multi-dimensional ODS media. | Shisheng Lin Hang Lin Chonggeng Ma Yao Cheng Sizhe Ye Fulin Lin Renfu Li Ju Xu Yuansheng Wang | 2020 | Light(Science & Applications)2020，9，1： | 12 |
| 7 | Phosphorus Speciation in Wetland Sediments of Zhujiang (Pearl) River Estuary,China显示文摘Phosphorus fractions and adsorption-release characteristics of sediments in the Zhujiang(Pearl) River estuary wetland were investigated.Results showed that the total phosphorus(TP) content in surface sediments ranged from 648.9 mg/kg to 1064.0 mg/kg;inorganic phosphorus(IP) was the major fraction of TP and ranged from 422.5 mg/kg to 643.9 mg/kg.Among the inorganic phosphorus,the main fractions were phosphorus bound to Al and Fe(Fe/Al-P),and calcium-bound phosphorus(Ca-P),accounting for 23%–42% and 21%–67% of IP,respectively.The vertical distribution of TP contents were significantly positive correlated with organic phosphorus(Org-P) and Fe/Al-P contents.The bio-available phosphorus contents in vertical sediments varied from 128.6 mg/kg to 442.9 mg/kg,mainly existed in Fe-Al/P fraction,and increased from the bottom to top sediments.The transport of phosphorus in sediment-water interface was controlled by the soil characteristics.The active Fe and Al content was considered as the main factor that determines adsorption capacity in vegetated marsh wetland.The P buffering capacity of the sediments in vegetated marsh wetland was greater than that in mudflat wetland.The potential risk of eutrophication in the study area is high.Reducing terrestrial phosphorus discharge and preventing the sediment Fe/Al-P release to the interstitial water are the possible solutions to reduce the risk of eutrophication in estuary wetlands,and planting vegetation in estuary wetland can also reduce the release of phosphorus in surface sediment. | WANG Lili YE Mei LI Qusheng ZOU Hang ZHOU Yongsheng | 2013 | Chinese Geographical Science2013，23，5： | 11 |
| 8 | Spontaneous Coronary Artery Dissection as a Cause of Acute Myocardial Infarction in Young Female Population: A Single-center Study显示文摘 | Pei-Na Meng Chen Xu Wei You Zhi-Ming Wu Du-Jiang Xie Hang Zhang Chang Pan Fei Ye | 2017 | Chinese Medical Journal2017，，13： | 10 |
| 9 | Albumin and magnetic resonance imaging-liver volume to identify hepatitis B-related cirrhosis and esophageal varices显示文摘AIM:To investigate whether liver lobe volume and albumin(ALB) could predict the presence and severity of liver cirrhosis,and esophageal varices.METHODS:Seventy-one cirrhotic patients with hepatitis B and 21 healthy individuals were enrolled in this study.All the participants underwent abdominal enhanced magnetic resonance imaging to measure each liver lobe volume,and biochemical workup for testing ALB and Child-Pugh class.All cirrhotic patients underwent upper gastrointestinal endoscopy to show the presence of cirrhotic esophageal varices.Right liver lobe volume(RV),left medial liver lobe volume(LMV),left lateral liver lobe volume(LLV),and caudate lobe volume(CV) were measured using enhanced magnetic resonance imaging.The ratios of RV to ALB(RV/ALB),LMV to ALB(LMV/ALB),LLV to ALB(LLV/ALB) and CV to ALB(CV/ALB) were calculated.Statistical analyses were performed to determine whether and how the combination of liver lobe volume measured using magnetic resonance imaging and albumin could predict the presence and severity of liver cirrhosis,and the presence of esophageal varices.RESULTS:RV,LMV,LLV and CV decreased(r =-0.51-0.373; all P < 0.05),while RV/ALB increased(r = 0.424; P < 0.05),with the progress of Child-Pugh classof liver cirrhosis.RV,LMV,CV,LLV/ALB and CV/ALB could identify presence of liver cirrhosis; LLV and LMV could distinguish Child-Pugh class A from B; RV,LMV,LLV,CV,RV/ALB and LLV/ALB could distinguish class A from C; RV and LLV/ALB could differentiate B from C; and RV,RV/ALB and CV/ALB could identify presence of esophageal varices(all P < 0.05).Among these parameters,CV/ALB could best identify the presence of liver cirrhosis,with an area under receiver operating characteristic curve(AUC) of 0.860,a sensitivity of 82.0% and a specificity of 83.0%.LLV could best distinguish class A from B,with an AUC of 0.761,a sensitivity of 74.4% and a specificity of 73.1%.RV could best distinguish class A from C,with an AUC of 0.900,a sensitivity of 90.3% and a specificity of 84.5%.LLV/ALB could best distinguish class B from C,with an AUC of 0.900,a sensitivity of 93.8% and a specificity of 81.5%.RV/ALB could best identify esophageal varices,with an AUC of 0.890,a sensitivity of 80.0% and a specificity of 83.5%.CONCLUSION:The combination of liver lobe volume and ALB has potential to identify presence and severity of cirrhosis,and presence of esophageal varices. | Hang Li Tian-Wu Chen Zhen-Lin Li Xiao-Ming Zhang Cheng-Jun Li Xiao-Li Chen Guang-Wen Chen Jia-Ni Hu Yong-Quan Ye | 2015 | World Journal of Gastroenterology2015，21，3： | 9 |
| 10 | Flunarizine inhibits sensory neuron excitability by blocking voltage-gated Na+ and Ca2+ currents in trigeminal ganglion neurons显示文摘背景尽管 flunarizine 广泛地与清楚的成功为周期性偏头痛预防被使用了,它在周期性偏头痛预防的行动的机制不是完全这研究的 understood.The 目的是在tetrodotoxin抵抗的Na+隧道和尖锐地孤立的老鼠的高电压的激活的Ca2+隧道上调查 flunarizine 的效果三叉的在三叉的中心神经原的中心 neurons.Methods 钠水流和钙水流用整个房间的补丁夹钳 recordin | YE Qing WANG Qiang YAN Lan-yun WU Wen-hui LIU Sha XIAO Hang WAN Qi | 2011 | Chinese Medical Journal2011，，17： | 7 |
| 11 | Clinical applications of exosome membrane proteins显示文摘Extracellular vesicles(EVs)are small membranous particles that can mediate cell-to-cell communication and which are divided into at least three categories according to their subcellular origin and size:exosomes,microvesicles,and apoptotic bodies.Exosomes are the smallest(30–150 nm)of these EVs,and play an important role in EV-mediated cell-to-cell interactions,by transferring proteins,nucleic acids and,lipids from their parental cells to adjacent or distant cells to alter their phenotypes.Most exosome studies in the past two decades have focused on their nucleic acid composition and their transfer ofmRNAs and microRNAs to neighboring cells.However,exosomes also carry specific membrane proteins that can identify the physiological and pathological states of their parental cells or indicate their preferential target cells or tissues.Exosome membrane protein expression can also be directly employed or modified to allow exosomes to serve as drug delivery systems and therapeutic platforms,including in targeted therapy approaches.This review will briefly summarize information on exosome membrane proteins components and their role in exosome–cell interactions,including proteins associated with specific cell-interactions and diseases,and the potential for using exosome membrane proteins in therapeutic targeting approaches. | Qian Hu Hang Su Juan Li Christopher Lyon Wenfu Tang Meihua Wan Tony Ye Hu | 2020 | Precision Clinical Medicine2020，3，1： | 6 |
| 12 | Elevated skin autofluorescence is strongly associated with foot ulcers in patients with diabetes: a cross-sectional, observational study of Chinese subjects显示文摘Objective: This study was designed to evaluate the association between skin autofluorescence (AF), an indicator of advanced glycation end-products (AGEs), and foot ulcers in subjects with diabetes. Methods: In this study, 195 Chinese diabetic subjects were examined. Their feet were examined regardless of whether an ulcer was present or not. Skin AF was measured with an AGE reader. Demographic characteristics and blood data were recorded. Results: The mean values of skin AF were 2.29±0.47 for subjects without foot ulcers, and 2.80±0.69 for those with foot ulcers, a significant difference (P<0.05). Skin AF was strongly correlated with age and duration of diabetes. After adjusting for these factors, multivariate logistic regression showed that skin AF was independently associated with foot ulcerations. Conclusions: Skin AF is independently associated with diabetic foot ulcerations. It might be a useful screening method for foot ulceration risk of diabetic patients. | Hang HU Chun-mao HAN Xin-lei HU Wan-lan YE Wen-juan HUANG Andries J.SMIT | 2012 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2012，13，5： | 6 |
| 13 | High accuracy in automatic detection of atrial fibrillation for Holter monitoring显示文摘Atrial fibrillation(AF) has been considered as a growing epidemiological problem in the world,with a substantial impact on morbidity and mortality.Ambulatory electrocardiography(e.g.,Holter) monitoring is commonly used for AF diagnosis and therapy and the automated detection of AF is of great significance due to the vast amount of information provided.This study presents a combined method to achieve high accuracy in AF detection.Firstly,we detected the suspected transitions between AF and sinus rhythm using the delta RR interval distribution difference curve,which were then classified by a combination analysis of P wave and RR interval.The MIT-BIH AF database was used for algorithm validation and a high sensitivity and a high specificity(98.2% and 97.5%,respectively) were achieved.Further,we developed a dataset of 24-h paroxysmal AF Holter recordings(n=45) to evaluate the performance in clinical practice,which yielded satisfactory accuracy(sensitivity=96.3%,specificity=96.8%). | Kai JIANG Chao HUANG Shu-ming YE Hang CHEN | 2012 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2012，13，9： | 6 |
| 14 | Lipid nanoparticle-encapsulated mRNA antibody provides longterm protection against SARS-CoV-2 in mice and hamsters显示文摘Monoclonal antibodies represent important weapons in our arsenal to against the CO VID-19 pandemic.However,this potential is severely limited by the time-consuming process of developing effective antibodies and the relative high cost of manufacturing.Herein,we present a rapid and cost-effective lipid nanoparticle(LNP)encapsulated-mRNA platform for in vivo delivery of SARS-CoV-2 neutralization antibodies.Two mRNAs encoding the light and heavy chains of a potent SARS-CoV-2 neutralizing antibody HB27,which is currently being evaluated in clinical trials,were encapsulated into clinical grade LNP formulations(named as mRNA-HB27-LNP).I n vivo characterizatio n dem on strated that intravenous administratio n of mRNA-HB27-LNP in mice resulted in a longer circulating half-life compared with the original HB27 antibody in protein format.More importantly,a single prophylactic administration of mRNA-HB27-LNP provided protection against SARS-CoV-2 challenge in mice at 1,7 and even 63 days post administration.In a close contact transmission model,prophylactic administration of mRNA-HB27-LNP prevented SARS-CoV-2 in fecti on betwee n hamsters in a dose-depende nt mariner.Overall,our results dem on strate a superior Ion g-term protect!on agai nst SARS-CoV-2 conferred by a single administration of this unique mRNA antibody,highlight!ng the potential of this universal platform for antibody-based disease prevention and therapy against COVID-19 as well as a variety of other infectious diseases. | Yong-Qiang Deng Na-Na Zhang Yi-Fei Zhang Xia Zhong Sue Xu Hong-Ying Qiu Tie-Cheng Wang Hui Zhao Chao Zhou Shu-Long Zu Qi Chen Tian-Shu Cao Qing Ye Hang Chi Xiang-Hui Duan Dan-Dan Lin Xiao-Jing Zhang Liang-Zhi Xie Yu-Wei Gao Bo Ying Cheng-Feng Qin | 2022 | Cell Research2022，32，4： | 5 |
| 15 | MicroRNA-99b-3p promotes angiotensin II-induced cardiac fibrosis in mice by targeting GSK-3β显示文摘Cardiac fibrosis is a typical pathological change in various cardiovascular diseases. Although it has been recognized as a crucial risk factor responsible for heart failure, there is still a lack of effective treatment. Recent evidence shows that microRNAs (miRNAs) play an important role in the development of cardiac fibrosis and represent novel therapeutic targets. In this study we tried to identify the cardiac fibrosis-associated miRNA and elucidate its regulatory mechanisms in mice. Cardiac fibrosis was induced by infusion of angiotensin II (Ang II, 2 mg·kg^(−1)·d^(−1)) for 2 weeks via osmotic pumps. We showed that Ang II infusion induced cardiac disfunction and fibrosis accompanied by markedly increased expression level of miR-99b-3p in heart tissues. Upregulation of miR-99b-3p and fibrotic responses were also observed in cultured rat cardiac fibroblasts (CFs) treated with Ang II (100 nM) in vitro. Transfection with miR-99b-3p mimic resulted in the overproduction of fibronectin, collagen I, vimentin and α-SMA, and facilitated the proliferation and migration of CFs. On the contrary, transfection with specific miR-99b-3p inhibitor attenuated Ang II-induced fibrotic responses. Similarly, intravenous injection of specific miR-99b-3p antagomir could prevent Ang II-infused mice from cardiac dysfunction and fibrosis. We identified glycogen synthase kinase-3 beta (GSK-3β) as a direct target of miR-99b-3p. In CFs, miR-99b-3p mimic significantly reduced the expression of GSK-3β, leading to activation of its downstream profibrotic effector Smad3, whereas miR-99b-3p inhibitor caused anti-fibrotic effects. GSK-3β knockdown ameliorated the anti-fibrotic role of miR-99b-3p inhibitor. These results suggest that miR-99b-3p contributes to Ang II-induced cardiac fibrosis at least partially through GSK-3β. The modulation of miR-99b-3p may provide a new approach for tackling fibrosis-related cardiomyopathy. | You-hui Yu Yu-hong Zhang Yan-qing Ding Xue-ying Bi Jing Yuan Hang Zhou Pan-xia Wang Li-li Zhang Jian-tao Ye | 2021 | Acta Pharmacologica Sinica2021，42，5： | 5 |
| 16 | Capillary blood flow in patients with dysmenorrhea treated with acupuncture显示文摘OBJECTIVE: To observe capillary blood flow at acupoints during acupuncture treatment of primary dysmenorrhea and gain new insights into its analgesic mechanism. METHODS: Patients with primary dysmenorrhea were enrolled and randomly assigned to a treatment or control group. Subjects' symptoms were differentiated into variousTraditional Chinese Medicine(TCM) syndromes and treated for 10 sessions with puncturing acupuncture or self-pressing right-hand Hegu(LI 4), adding other acupoints based on syndrome. Laser speckle was used to compare the change in the vasomotor amplitude and perfusion of the capillaries in Hegu(LI 4) before and during the treatment. Each subject was required to finish the period pain symptoms observation form, verbal rating scales, numerical rating scale, pain rating index, face rating scale, Zung self-rating depression scale, Zung self-rating anxiety scale, and numerical rating scale before and after treatments. RESULTS: After 10 sessions, the symptom scores, pain index(PI), and visual analog scale(VAS) decreased significantly in treatment group. The volume of blood flow in Hegu(LI 4) declined slightly. No significant evidence supported that needling caused capillary contraction, but the capillary vasomotor amplitude at Hegu(LI 4) increased remarkably. CONCLUSION: Acupuncture can increase the capillary blood flow, thus promoting the flow of Qi and blood in terms of TCM theory, which facilitates pain relief. | Tao Huang Lijian Yang Shuyong Jia Xiang Mu Mozheng Wu Hang Ye Weizhe Liu Xinnong Cheng | 2013 | Journal of Traditional Chinese Medicine2013，33，6： | 5 |
| 17 | Influences of Quinclorac on Culturable Microorganisms and Soil Respiration in Flooded Paddy Soil显示文摘Objective To investigate the potential effects of herbicide quinclorac (3,7-dichloro-8-quinoline-carboxylic) on the culturable microorganisms in flooded paddy soil. Methods Total soil aerobic bacteria, actinomycetes and fungi were counted by a 10-fold serial dilution plate technique. | ZHEN-MEI LU, HANG MIN , AND YANG-FANG YE Institute of Microbiology, College of Life Science, Zhejiang University, Hangzhou 310029, Zhejiang, China | 2003 | Biomedical and Environmental Sciences2003，16，4： | 5 |
| 18 | Glomerular Disease Associated with Takayasu Arteritis:6 Cases Analysis and Review of the Literature显示文摘Objective To evaluate the clinical features,renal histopathology and therapeutic response to glucocorticoid and immunosuppressive agents in patients with glomerular disease associated with Takayasu arteritis(TA).Methods Patients with TA and renal biopsy-confirmed glomerular disease were investigated retrospectively.None of them had renal artery stenosis or occlusive changes.Results Six patients with glomerulopathy,accounting for 3.75% of the 160 TA patients admitted to our hospital at the same period,were analyzed.All of them were females with a mean age of 35.5 ± 10.0 years.Four cases presented with lower extremity edema.Laboratory tests showed that one was nephrotic syndrome,three were nephrotic range proteinuria,and two of them had mild renal dysfunction.The other two patients were asymptomatic microscopic hematuria and proteinuria.Renal pathology revealed mild immunoglobulin A nephropathy in two cases,mild mesangial proliferative glomerulonephritis(GN),membranoproliferative GN,minimal change disease,and fibrillary GN in one case respectively.Five cases received glucocorticoids and cyclophosphamide therapy.Proteinuria and microscopic hematuria disappeared in 2 to 4 weeks after the initiation of therapy in three cases.The patient with membranoproliferative GN also reached complete remission of proteinuria and recovered renal function 6 months after the treatment.Conclusions TA may induce glomerular disease as a part of its histological spectrum.Apart from ischemic glomerular disease,glomerular disease should be suspected when TA patients have microscopic hematuria or proteinuria,that may be therapeutically responsive to glucocorticoids and immunosuppressive agent in relative early phase. | Xue-mei Li Wen-ling Ye Yu-bing Wen Hang Li Li-meng Chen Dong-yan Liu Xue-jun Zeng Xue-wang Li | 2009 | Chinese Medical Sciences Journal2009，24，2： | 4 |
| 19 | The oncoprotein HBXIP promotes human breast cancer growth through down-regulating p53 via miR-18b/MDM2 and pAKT/MDM2 pathways显示文摘 | Hang Li Zhen Wang Mian Jiang Run-ping Fang Hui Shi Yu Shen Xiao-li Cai Qian Liu Kai Ye Sai-jun Fan Wei-ying Zhang Li-hong Ye | 2018 | Acta Pharmacologica Sinica2018，39，11： | 4 |
| 20 | Nodeless superconductivity in the kagome metal CsV_(3)Sb_(5)显示文摘The recently discovered kagome metal series AV3Sb5(A=K, Rb, Cs) exhibits topologically nontrivial band structures, chiral charge order and superconductivity, presenting a unique platform for realizing exotic electronic states. The nature of the superconducting state and the corresponding pairing symmetry are key questions that demand experimental clarification. Here, using a technique based on the tunneling diode oscillator, the magnetic penetration depth ?λ(T) of CsV3Sb5 was measured down to 0.07 K. A clear exponential behavior in ?λ(T) with marked deviations from a T or T2 temperature dependence was observed at low temperatures, indicating an absence of nodal quasiparticles. Temperature dependence of the superfiuid density and electronic specific heat can be described by two-gap s-wave superconductivity, consistent with the presence of multiple Fermi surfaces in CsV3Sb5. These results evidence nodeless superconductivity in CsV3Sb5 under ambient pressure, and constrain the allowed pairing symmetry. | Weiyin Duan Zhiyong Nie Shuaishuai Luo Fanghang Yu Brenden R.Ortiz Lichang Yin Hang Su Feng Du An Wang Ye Chen Xin Lu Jianjun Ying Stephen DWilson Xianhui Chen Yu Song Huiqiu Yuan | 2021 | Science China(Physics,Mechanics & Astronomy)2021，64，10： | 3 |
您所在的机构暂未开通此部分的功能，请联系管理员