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| 1 | Serum MicroRNA Signatures Identified in a Genome-Wide Serum MicroRNA Expression Profiling Predict Survival of Non-Small-Cell Lung Cancer(摘要)显示文摘 | Hu, ZB Chen, X Zhao, Y Tian, T Jin, GF Shu, YQ Chen, YJ Xu, L Zen, K Zhang, CY Shen, HB | 2010 | 南京医科大学学报(自然科学版)2010，30，7： | 141 |
| 2 | Diagnosis, treatment, and prevention of 2019 novel coronavirus infection in children: experts' consensus statement显示文摘Since the outbreak of 2019 novel coronavirus infection (2019-nCoV) in Wuhan City,China,by January 30,2020,a total of 9692 confirmed cases and 15,238 suspected cases have been reported around 31 provinces or cities in China.Among the confirmed cases,1527 were severe cases,171 had recovered and been discharged at home,and 213 died.And among these cases,a total of 28 children aged from 1 month to 17 years have been reported in China.For standardizing prevention and management of 2019-nCoV infections in children,we called up an experts' committee to formulate this experts' consensus statement.This statement is based on the Novel Coronavirus Infection Pneumonia Diagnosis and Treatment Standards (the fourth edition) (National Health Committee) and other previous diagnosis and treatment strategies for pediatric virus infections.The present consensus statement summarizes current strategies on diagnosis,treatment,and prevention of 2019-nCoV infection in children. | Kunling Shen Yonghong Yang Tianyou Wang Dongchi Zhao Yi Jiang Runming Jin Yuejie Zheng Baoping Xu Zhengde Xie Likai Lin Yunxiao Shang Xiaoxia Lu Sainan Shu Yan Bai Jikui Deng Min Lu Leping Ye Xuefeng Wang Yongyan Wang Liwei Gao | 2020 | World Journal of Pediatrics2020，16，3： | 56 |
| 3 | Diagnosis and treatment recommendations for pediatric respiratory infection caused by the 2019 novel coronavirus显示文摘Since December 2019,an epidemic caused by novel coronavirus (2019-nCoV) infection has occurred unexpectedly in China.As of 8 pm,31 January 2020,more than 20 pediatric cases have been reported in China.Of these cases,ten patients were identified in Zhejiang Province,with an age of onset ranging from 112 days to 17 years.Following the latest National recommendations for diagnosis and treatment of pneumonia caused by 2019-nCo V (the 4th edition) and current status of clinical practice in Zhejiang Province,recommendations for the diagnosis and treatment of respiratory infection caused by 2019-nCoV for children were drafted by the National Clinical Research Center for Child Health,the National Children's Regional Medical Center,Children's Hospital,Zhejiang University School of Medicine to further standardize the protocol for diagnosis and treatment of respiratory infection in children caused by 2019-nCoV. | Zhi-Min Chen Jun-Fen Fu Qiang Shu Ying-Hu Chen Chun-Zhen Hua Fu-Bang Li Ru Lin Lan-Fang Tang Tian-Lin Wang Wei Wang Ying-Shuo Wang Wei-Ze Xu Zi-Hao Yang Sheng Ye Tian-Ming Yuan Chen-Mei Zhang Yuan-Yuan Zhang | 2020 | World Journal of Pediatrics2020，16，3： | 45 |
| 4 | SlMYB75,an MYB-type transcription factor,promotes anthocyanin accumulation and enhances volatile aroma production in tomato fruits显示文摘Genetic manipulation of genes to upregulate specific branches of metabolic pathways is a method that is commonly used to improve fruit quality.However,the use of a single gene to impact several metabolic pathways is difficult.Here,we show that overexpression of the single gene SlMYB75(SlMYB75-OE)is effective at improving multiple fruit quality traits.In these engineered fruits,the anthocyanin content reached 1.86mg g−1 fresh weight at the red-ripe stage,and these SlMYB75-OE tomatoes displayed a series of physiological changes,including delayed ripening and increased ethylene production.In addition to anthocyanin,the total contents of phenolics,flavonoids and soluble solids in SlMYB75-OE fruits were enhanced by 2.6,4,and 1.2 times,respectively,compared to those of wild-type(WT)fruits.Interestingly,a number of aroma volatiles,such as aldehyde,phenylpropanoid-derived and terpene volatiles,were significantly increased in SlMYB75-OE fruits,with some terpene volatiles showing more than 10 times higher levels than those in WT fruits.Consistent with the metabolic assessment,transcriptomic profiling indicated that the genes involved in the ethylene signaling,phenylpropanoid and isoprenoid pathways were greatly upregulated in SlMYB75-OE fruits.Yeast one-hybrid and transactivation assays revealed that SlMYB75 is able to directly bind to the MYBPLANT and MYBPZM cis-regulatory elements and to activate the promoters of the LOXC,AADC2 and TPS genes.The identification of SlMYB75 as a key regulator of fruit quality attributes through the transcriptional regulation of downstream genes involved in several metabolic pathways opens new avenues towards engineering fruits with a higher sensory and nutritional quality. | Wei Jian Haohao Cao Shu Yuan Yudong Liu Juanfang Lu Wang Lu Ning Li Jianhui Wang Jian Zou Ning Tang Chan Xu Yulin Cheng Yanqiang Gao Wanpeng Xi Mondher Bouzayen Zhengguo Li | 2019 | Horticulture Research2019，6，1： | 39 |
| 5 | Microstructural Evolution and Mechanical Properties of Inconel 625 Alloy during Pulsed Plasma Arc Deposition Process显示文摘Pulsed plasma arc deposition(PPAD),which combines pulsed plasma cladding with rapid prototyping,is a promising technology for manufacturing near net shape components due to its superiority in cost and convenience of processing.In the present research,PPAD was successfully used to fabricate the Ni-based superalloy Inconel 625 components.The microstructures and mechanical properties of deposits were investigated by scanning electron microscopy(SEM),optical microscopy(OM),transmission electron microscopy(TEM) with energy dispersive spectrometer(EDS),microhardness and tensile testers.It was found that the as-deposited structure exhibited homogenous columnar dendrite structure,which grew epitaxially along the deposition direction.Moreover,some intermetallic phases such as Laves phase,minor MC(NbC, TiC) carbides and needle-likeδ-Ni_3Nb were observed in y-Ni matrix.Precipitation mechanism and distribution characteristics of these intermetallic phases in the as-deposited 625 alloy sample were analyzed.In order to evaluate the mechanical properties of the deposits,microhardness was measured at various location (including transverse plane and longitudinal plane).The results revealed hardness was in the range of 260—285 HV_(0.2).In particular,microhardness at the interface region between two adjacent deposited layers was slightly higher than that at other regions due to highly refined structure and the disperse distribution of Laves particles.Finally,the influence of precipitation phases and fabrication strategies on the tensile properties of the as-deposited samples was investigated.The failure modes of the tensile specimens were analyzed with fractography. | Fujia Xu Yaohui Lv Yuxin Liu Fengyuan Shu Peng He Binshi Xu | 2013 | Journal of Materials Science & Technology2013，29，5： | 33 |
| 6 | Influence of Atmospheric Particulate Matter on Ozone in Nanjing,China:Observational Study and Mechanistic Analysis显示文摘Particulate matter with diameters of 2.5 μm or smaller(PM_(2.5)) and ozone(O_3) are major pollutants in the urban atmosphere. PM_(2.5) can affect O_3 by altering the photolysis rate and heterogeneous reactions. However, these two processes and their relative importance remain uncertain. In this paper, with Nanjing in China as the target city, we investigate the characteristics and mechanism of interactions between particles and O_3 based on ground observations and numerical modeling.In 2008, the average concentrations of PM_(2.5) and O_3 at Caochangmen station are 64.6 ± 47.4 μg m^(-3) and 24.6 ± 22.8 ppb,respectively, while at Pukou station they are 94.1 ± 63.4 μg m^(-3) and 16.9 ± 14.9 ppb. The correlation coefficient between PM_(2.5) and O_3 is -0.46. In order to understand the reaction between PM_(2.5) and O_3, we construct a box model, in which an aerosol optical property model, ultraviolet radiation model, gas phase chemistry model, and heterogeneous chemistry model,are coupled. The model is employed to investigate the relative contribution of the aforementioned two processes, which vary under different particle concentrations, scattering capability and VOCs/NOxratios(VOCs: volatile organic compounds;NOx: nitric oxide and nitrogen dioxide). Generally, photolysis rate effect can cause a greater O_3 reduction when the particle concentrations are higher, while heterogeneous reactions dominate O_3 reduction with low-level particle concentrations.Moreover, in typical VOC-sensitive regions, O_3 can even be increased by heterogeneous reactions. In Nanjing, both processes lead to O_3 reduction, and photolysis rate effect is dominant. Our study underscores the importance of photolysis rate effect and heterogeneous reactions for O_3, and such interaction processes should be fully considered in future atmospheric chemistry modeling. | Yawei QU Tijian WANG Yanfeng CAI Shekou WANG Pulong CHEN Shu LI Mengmeng LI Cheng YUAN Jing WANG Shaocai XU | 2018 | Advances in Atmospheric Sciences2018，35，11： | 33 |
| 7 | Prognostic value of preoperative serum tumor markers in gastric cancer显示文摘AIM:To evaluate the prognostic value of preoperative carcinoembryonic antigen(CEA), carbohydrate antigen(CA)19-9, and CA50 in patients undergoing D2 resection.METHODS:We evaluated 363 patients with gastric cancer who underwent gastrectomy at our hospital from January 2006 to December 2009. Blood samples were obtained from each patient within 1 wk before surgery. The cut-off values for serum CEA, CA19-9,and CA50 were 5 ng/mL, 37 U/mL, and 20 U/mL, respectively. The correlation between preoperative tumor marker levels and prognosis was studied by means of univariate and multivariate analyses.RESULTS:The preoperative serum positive rates of CEA, CA19-9 and CA50 were 24.0%, 18.9% and24.5%, respectively. The positivity rate of serum CEA was significantly correlated with age(P < 0.001), sex(P = 0.022), tumor size(P = 0.007) and depth of invasion(P = 0.018); CA19-9 with tumor size(P = 0.042)and lymph node metastasis(P < 0.001); and CA50 onlywith lymph node metastasis(P = 0.001). In multivariate analysis, tumor size, T category, N category, vascular or neural invasion, and adjuvant chemotherapy were independent prognostic factors for overall survival. CA19-9 had an independent prognostic significance in patients without adjuvant chemotherapy(P = 0.027).CONCLUSION:Preoperative serum CEA, CA19-9 and CA50 are prognostic in patients with gastric cancer. Only CA19-9 is an independent prognostic factor after surgery without adjuvant chemotherapy. | Ze-Bo Huang Xin Zhou Jun Xu Yi-Ping Du Wei Zhu Jian Wang Yong-Qian Shu Ping Liu | 2014 | World Journal of Clinical Oncology2014，5，2： | 30 |
| 8 | Antidiabetic Effects of Gegen Qinlian Decoction via the Gut Microbiota Are Attributable to Its Key Ingredient Berberine显示文摘Gegen Qinlian Decoction(GQD),a traditional Chinese medicine(TCM)formula,has long been used for the treatment of common metabolic diseases,including type 2 diabetes mellitus.However,the main limitation of its wider application is ingredient complexity of this formula.Thus,it is critically important to identify the major active ingredients of GQD and to illustrate mechanisms underlying its action.Here,we compared the effects of GQD and berberine,a hypothetical key active pharmaceutical ingredient of GQD,on a diabetic rat model by comprehensive analyses of gut microbiota,short-chain fatty acids,proinflammatory cytokines,and ileum transcriptomics.Our results show that berberine and GQD had similar effects on lowering blood glucose levels,modulating gut microbiota,inducing ileal gene expression,as well as relieving systemic and local inflammation.As expected,both berberine and GQD treatment significantly altered the overall gut microbiota structure and enriched many butyrate-producing bacteria,including Faecalibacterium and Roseburia,thereby attenuating intestinal inflammation and lowering glucose.Levels of short-chain fatty acids in rat feces were also significantly elevated after treatment with berberine or GQD.Moreover,concentration of serum proinflammatory cytokines and expression of immune-related genes,including Nfkb1,Stat1,and Ifnrg1,in pancreatic islets were significantly reduced after treatment.Our study demonstrates that the main effects of GQD can be attributed to berberine via modulating gut microbiota.The strategy employed would facilitate further standardization and widespread application of TCM in many diseases. | Xizhan Xu Zezheng Gao Fuquan Yang Yingying Yang Liang Chen Lin Han Na Zhao Jiayue Xu Xinmiao Wang Yue Ma Lian Shu Xiaoxi Hu Na Lyu Yuanlong Pan Baoli Zhu Linhua Zhao Xiaolin Tong Jun Wang | 2020 | Genomics, Proteomics & Bioinformatics2020，18，6： | 29 |
| 9 | Efficacy and safety of a novel anti-HER2 therapeutic antibody RC48 in patients with HER2-overexpressing,locally advanced or metastatic gastric or gastroesophageal junction cancer:a single-arm phase II study显示文摘Background:Current treatment options for human epidermal growth factor receptor 2(HER2)-overexpressing gastric cancer at third-line have shown limited clinical benefit.Further,there is no specific treatment for HER2 immunohistochemistry(IHC)2+and fluorescence in-situ hybridization-negative patients.Here,we report the efficacy and safety of a novel anti-HER2 antibody RC48 for patients with HER2-overexpressing,advanced gastric or gastroesophageal junction cancer.Methods:Patients with HER2-overexpressing(IHC 2+or 3+),locally advanced or metastatic gastric or gastroesophageal junction cancer who were under at least second-line therapy were eligible and received RC482.5 mg/kg alone every 2 weeks.The primary endpoint was the objective response rate(ORR)assessed by an independent review committee.Secondary endpoints included progressionfree survival(PFS),overall survival(OS),duration of response,time to progression,disease control rate,and safety.Results:Of 179 patients screened,125 were eligible and received RC48 treatment.The ORR was 24.8%(95%confidence interval[CI]:17.5%-33.3%).The median PFS and OS were 4.1 months(95%CI:3.7-4.9 months)and 7.9 months(95%CI:6.7-9.9 months),respectively.The most frequently reported adverse events were decreased white blood cell count(53.6%),asthenia(53.6%),hair loss(53.6%),decreased neutrophil count(52.0%),anemia(49.6%),and increased aspartate aminotransferase level(43.2%).Serious adverse events(SAEs)occurred in 45(36.0%)patients,and RC48-related SAEs were mainly decreased neutrophil count(3.2%).Seven patients had adverse events that led to death were not RC48-related.Conclusions:RC48 showed promising activity with manageable safety,suggesting potential application in patients with HER2-overexpressing,advanced gastric or gastroesophageal junction cancer who have previously received at least two lines of chemotherapy. | Zhi Peng Tianshu Liu Jia Wei Airong Wang Yifu He Liuzhong Yang Xizhi Zhang Nanfeng Fan Suxia Luo Zhen Li Kangsheng Gu Jianwei Lu Jianming Xu Qingxia Fan Ruihua Xu Liangming Zhang Enxiao Li Yuping Sun Guohua Yu Chunmei Bai Yong Liu Jiangzheng Zeng Jieer Ying Xinjun Liang Nong Xu Chao Gao Yongqian Shu Dong Ma Guanghai Dai Shengmian Li Ting Deng Yuehong Cui Jianmin Fang Yi Ba Lin Shen | 2021 | Cancer Communications2021，41，11： | 29 |
| 10 | Quantitative real-time RT-PCR detection for CEA, CK20 and CK19 mRNA in peripheral blood of colorectal cancer patients显示文摘This study is aimed at establishing a sensitive approach to detect disseminated tumor cells in peripheral blood and evaluate its clinical significance. A total of 198 blood samples including 168 from colorectal carcinoma (CRC) patients and 30 from healthy volunteers were examined by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) to evaluate the expression of carcinoembryonic antigen (CEA), cytokeratin 20 (CK20) and cytokeratin 19 (CK19) mRNA. CEA mRNA was detected in 35.8% of patients and 3.3% of controls, CK20 mRNA in 28.3% of patients and 6.7% of controls, and CK19 mRNA in 41.9% of patients and 3.3% of controls. CEA and CK20 mRNA positive ratio increased with the advancing Dukes stages, but there was no significant difference in positive ratio between any two stages (P>0.05). Also, relatively high positive ratio of CEA, CK20 and CK19 mRNA expression was observed in some CRC patients with earlier Dukes stages. A higher positive ratio was obtained when two or three detection markers were combined compared to a single marker. Our study indicates that quanti-tative real-time RT-PCR detection for CEA, CK20 and CK19 mRNA in peripheral blood is a valuable tool for monitoring early stage dissemination of CRC cells in blood circulation. | XU Dong LI Xu-fen ZHENG Shu JIANG Wen-zhi | 2006 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2006，7，6： | 27 |
| 11 | Oridonin induces apoptosis via PI3K/Akt pathway in cervical carcinoma HeLa cell line显示文摘瞄准:调查 oridonin 的导致 apoptosis 效果,从 Rabdosia rubescens 孤立的 diterpenoid,在人的颈的癌 HeLa,房间排队。方法:染色质的词法分析,原子冷凝作用,和破碎用染色的 Hoechst 33342 被监视。房间生存能力用 3- 被估计(4, 5-dimethylthiazol-(2 )-yl)-2, 5-diphenyl tetrazolium 溴化物(MTT ) 试金。在 HeLa 房间线的房间 apoptosis 和 apoptosis 相关的激活被流动血细胞计数并且西方的弄污评估。结果:Oridonin 在一个剂量依赖者和时间依赖者时尚压制了 HeLa 房间线的增长。Oridonin 处理在下面调整了蛋白质激酶 B (Akt ) 的激活,叉头框类 O (FOXO ) 抄写因素的表达式,和肝糖 synthase 激酶 3 (GSK3 ) 。Oridonin 也导致了 caspase-3 和 poly 腺苷 diphosphate- 核糖聚合酶劈开的激活伴随的细胞色素 c 的版本。另外, Z-D (OMe )-E(OMe)-V-D(OMe)- FMK (z-DEVD-fmk ) , caspases 的一个禁止者,阻止的 caspase-3 激活和废除导致 oridonin 的细胞死亡。最后, HeLa 房间的 oridonin 处理排队在下面调整了 apoptosis 蛋白质的禁止者的表示。结论:我们的结果证明导致 oridonin 的 apoptosis 包含了几条分子的小径。Oridonin 可以在 HeLa 房间线压制 phosphatidylinositol 3-kinase (Akt, FOXO,和 GSK3 ) 的组成地激活的目标,禁止 caspase 依赖的 apoptosis 的增长和正式就职。 | Hong-zhen HU Yue-bo YANG Xiang-dong XU Hong-wei SHEN Yi-min SHU Zi REN Xiao-mao LI Hui-ming SHEN Hai-tao ZENG | 2007 | Acta Pharmacologica Sinica2007，28，11： | 26 |
| 12 | Furazolidone-based triple and quadruple eradication therapy for Helicobacter pylori infection显示文摘AIM: To evaluate the efficacy of furazolidone-based triple and quadruple therapy in eradicating Helicobacter pylori(H. pylori) in a multi-center randomized controlled trial.METHODS: A total of 720 H. pylori positive patients with duodenal ulcer disease were enrolled at 10 different hospitals in Jiangxi province in China. The patients were randomly assigned to four treatment groups as follows: patients in Groups 1 and 3 received rabeprazole(10 mg), amoxicillin(1000 mg) and furazolidone(100 mg) twice daily for 7 and 10 d, respectively; patients in Groups 2 and 4 received rabeprazole(10 mg), bismuth(220 mg), amoxicillin(1000 mg) and furazolidone(100 mg) twice daily for 7 and 10 d, respectively. The primary outcome measure was H. pylori eradication rate 4 wk after treatment by intention-to-treat and per protocol analysis, while the secondary outcome measures were symptom and sign changes at the end of treatment and 4 wk after the end of treatment, as well as the proportion of patients who developed adverse events.RESULTS: The demographic data of the four groups were not significantly different. Overall, 666 patients completed the scheme and were re-assessed with the 13C-urea breath test. The intention-to-treat analysis of the H. pylori eradication rates in Groups 1, 2, 3 and 4 were 74.44%, 82.78%, 78.89% and 86.11%, respectively. The H. pylori eradication rate in Group 4 was significantly higher than that in Group 1. According tothe per protocol analysis, the H. pylori eradication rates in Groups 1, 2, 3 and 4 were 81.21%, 89.22%, 85.54% and 92.26%, respectively. The H. pylori eradication rate in Group 4 was significantly higher than that in Group 1. The number of adverse events was 15(8.3%), 16(8.9%), 15(8.3%) and 17(9.4%) in Groups 1, 2, 3 and 4, respectively, including dizziness, vomiting, diarrhea, nausea, skin rash, itchy skin, and malaise. The symptoms were relieved without special treatment in all of the patients.CONCLUSION: Both 7- and 10-d quadruple furazolidone-based therapies achieve satisfactory H. pylori eradication rates. | Yong Xie Yin Zhu Hong Zhou Zhi-Fa Lu Zhen Yang Xu Shu Xiao-Bai Guo Hui-Zhen Fan Jian-Hua Tang Xue-Ping Zeng Jian-Bo Wen Xiao-Qing Li Xing-Xing He Jiu-Hong Ma Dong-Sheng Liu Cai-Bin Huang Ning-Jian Xu Nong-Rong Wang Nong-Hua Lu | 2014 | World Journal of Gastroenterology2014，20，32： | 24 |
| 13 | Large Scale Photochemical Synthesis of M@TiO_(2) Nanocomposites (M = Ag, Pd, Au, Pt) and Their Optical Properties, CO Oxidation Performance, and Antibacterial Effect显示文摘Well-dispersed M@TiO_(2)(M=Ag,Pd,Au,Pt)nanocomposite particles with a diameter of 200-400 nm can be synthesized on a large scale by a clean photochemical route which does not require any additives using spherical rutile nanoparticles as a support.The sizes of Pt,Au,and Pd nanoparticles formed on the surface of TiO_(2) particles are about 1 nm,5 nm,and 5 nm,respectively,and the diameter of Ag nanoparticles is in the range 2-20 nm.Moreover,the noble metal nanoparticles have good dispersity on the particles of the TiO_(2) support,resulting in excellent catalytic activities.Complete conversion in catalytic CO oxidation is reached at temperatures as low as 333 and 363 K,respectively,for Pt@TiO_(2) and Pd@TiO_(2) catalysts.In addition,the antibacterial effects of the as-synthesized TiO_(2) nanoparticles,silver nanoparticles,and Au@TiO_(2) and Ag@TiO_(2) nanocomposites have been tested against Gram-negative Escherichia coli(E.coli)bacteria.The results demonstrate that the presence of the TiO_(2) matrix enhances the antibacterial effect of silver nanoparticles,and the growth of E.coli can be completely inhibited even if the concentration of Ag in Ag@TiO_(2) nanocomposite is very low(10μg/mL). | Shao Feng Chen Jian Ping Li Kun Qian Wei Ping Xu Yang Lu Wei Xin Huang Shu Hong Yu | 2010 | Nano Research2010，3，4： | 20 |
| 14 | Laser altimetry data of Chang'E-1 and the global lunar DEM model显示文摘The Laser AltiMeter (LAM), as one of the main payloads of Chang'E-1 probe, is used to measure the topography of the lunar surface. It performed the first measurement at 02:22 on November 28th, 2007. Up to December 4th 2008, the total number of measurements was approximately 9.12 million, covering the whole surface of the Moon. Using the LAM data, we constructed a global lunar Digtal Elevation Model (DEM) with 3 km spatial resolution. The model shows pronounced morphological characteristics, legible and vivid details of the lunar surface. The plane positioning accuracy of the DEM is 445 m (1σ), and the vertical accuracy is 60 m (1σ). From this DEM model, we measured the full range of the altitude difference on the lunar sur-face, which is about 19.807 km. The highest point is 10.629 km high, on a peak between crater Korolev and crater Dirichlet-Jackson at (158.656°W, 5.441°N) and the lowest point is -9.178 km in height, inside crater Antoniadi (172.413°W, 70.368°S) in the South Pole-Aitken Basin. By comparison, the DEM model of Chang'E-1 is better than the USA ULCN2005 in accuracy and resolution and is probably identical to the DEM of Japan SELENE, but the DEM of Chang'E-1 reveals a new lowest point, clearly lower than that of SELENE. | LI ChunLai1, REN Xin1, LIU JianJun1, ZOU XiaoDuan1, MU LingLi1, WANG JianYu2, SHU Rong2, ZOU YongLiao1, ZHANG HongBo1, Lü Chang1, LIU JianZhong1, ZUO Wei1, SU Yan1, WEN WeiBin1, BIAN Wei1, WANG Min1, XU Chun1, KONG DeQing1, WANG XiaoQian1, WANG Fang1, GENG Liang1, ZHANG ZhouBin1, ZHENG Lei1, ZHU XinYing1, LI JunDuo1 & OUYANG ZiYuan1 1 National Astronomical Observatories, Chinese Academy of Sciences, Beijing 100012, China 2 Shanghai Institute of Technical Physics, Chinese Academy of Sciences, Shanghai 200083, China | 2010 | Science China Earth Sciences2010，53，11： | 19 |
| 15 | Apoptosis induction with polo-like kinase-1 antisense phosph-orothioate oligodeoxynucleotide of colon cancer cell line SW480显示文摘AIM: To investigate the effects of polo-like kinase-1 (PLK1) antisense phosphorothioate oligodeoxynucleotide (ASODN) on apoptosis and cell cycle of human colon cancer cell line 5W480.METHODS: After SW480 colon cancer cells were transfected with PLK1 ASODN, Northern and Western blot analyses were used to examine PLK1 gene expression in cancer cells. We studied apoptosis using terminal uridine deoxynucleotidyl nick end labeling. Apoptosis and cell cycle of SW480 cells were examined by fluorescence-activated cell sorter scan.RESULTS: The levels of PLK1 mRNA and protein were greatly inhibited by PLK1 ASODN in SW480 cancer cells transfected with PLK1 ASODN. Apoptosis index (AI) induced PLK1 ASODN in a time- and dose-dependent manner.Results from FLM showed that sub-2N DNA content of transfected cancer cells was significantly increased and arrested at G2/M compared with control groups.CONCLUSION: PLK1 ASODN can induce apoptosis of human colon cancer cell line SW480. | Yu Fan Shu Zheng Ze-Feng Xu Jia-Yi Ding | 2005 | World Journal of Gastroenterology2005，11，29： | 18 |
| 16 | Pretreatment HIV drug resistance in adults initiating antiretroviral therapy in China,2017显示文摘Background:After the scale-up of antiretroviral therapy(ART)for HIV infected people,increasing numbers of patients have pretreatment drug resistance(PDR).In this study,the prevalence of PDR was evaluated in adults initiating antiretroviral therapy in China.Methods:Blood samples were obtained from 1943 patients who initiated antiretroviral therapy(ART)in 2017 from 13 provinces or cities in China.Pol sequences were used to analyze drug resistance and construct transmission networks.Logistic regression model was used to estimate the potential factors associated with PDR.Results:In total,1711 eligible patients(76.0%male;87.8%aged≥25 years)were included,of which 117(6.8%)had PDR.The highest rates of PDR were 12.2%in Liangshan Prefecture of Sichuan and 9.3 and 8.9%in Dehong and Lincang Prefecture of Yunnan.A multivariate logistic regression analysis revealed that PDR was significantly higher among intravenous drug users(adjusted Odds Ratio(aOR)=2.64,95%CI:1.57–4.44)and individuals from Liangshan,Dehong,and Lincang(aOR=2.04,95%CI:1.26–3.30).In total,754 sequences were used to generate 164 transmission networks.Five transmission networks had two or three sequences containing the same mutations,two networks contained subjects from Liangshan,and one network contained subjects from Dehong.Conclusions:Overall,the PDR prevalence was moderate,with a particularly high prevalence in areas with severe HIV epidemics.These results indicate the importance of continuous PDR monitoring in patients initiating antiretroviral therapy. | Rui-Hua Kang Shu-Jia Liang Yan-Ling Ma Shu Liang Lin Xiao Xin-Hui Zhang Hong-Yan Lu Xiao-Qin Xu Shui-Bin Luo Xiao-Guang Sun Lin Chen Jian-Mei He Guo-Hui Wu Ling-Jie Liao Hui Xing Yi-Ming Shao Yu-Hua Ruan | 2020 | Infectious Diseases of Poverty2020，9，3： | 17 |
| 17 | Association of Dietary Pattern during Pregnancy and Gestational Diabetes Mellitus: A Prospective Cohort Study in Northern China显示文摘Objective To examine the association of maternal dietary patterns during pregnancy with gestational diabetes mellitus(GDM) in northern China.Methods The dietary intakes of pregnant women were recorded twice by 24‐hour dietary recalls for three days prior to having been diagnosed with GDM, at 5‐15 and 24‐28 gestational weeks, respectively.GDM was diagnosed, and serum glycosylated hemoglobin(HbA1c) was measured at 24‐28 weeks.Dietary patterns were assessed by factor analysis. The association of the dietary pattern with GDM and HbA1c was examined by multiple logistic models.Results Of 753 participants, 64(8.5%) were diagnosed with GDM. Four dietary patterns were identified: Western pattern(dairy, baked/fried food and white meat), traditional pattern(light‐colored vegetables, fine grain, red meat and tubers), mixed pattern(edible fungi, shrimp/shellfish and red meat)and prudent pattern(dark‐colored vegetables and deep‐sea fish). Compared with the prudent pattern,both the Western pattern and the traditional pattern were associated with an increased risk of GDM(a OR = 4.40, 95% CI: 1.58‐12.22; a OR = 4.88, 95% CI: 1.79‐13.32) and a high level of HbA1c(a OR = 12.37,95% CI: 1.47‐103.91; a OR = 26.23, 95% CI: 2.54‐270.74). Compared to the lowest quartile(Q), Q3 of the Western pattern scores and Q3‐Q4 of the traditional pattern scores were associated with a higher risk of GDM.Conclusion The consumption of the Western pattern or the traditional pattern during pregnancy may increase the risk of GDM. | DU Hong Yi JIANG Hong O Karmin CHEN Bo XU Lin Ji LIU Shu Ping YI Jian Ping HE Geng Sheng QIAN Xu | 2017 | Biomedical and Environmental Sciences2017，30，12： | 16 |
| 18 | Preoperative assessment of hilar cholangiocarcinoma:combination of cholangiography and CT angiography显示文摘BACKGROUND:Hilar cholangiocarcinoma is one of the most difficult carcinomas to manage because of the location of the main tumor at the hepatic hilus and the complex anatomy of the biliary,arterial,and portal systems.To plan an operation,it is important to acquire accurate information about the relationship between hilar cholangiocarcinoma and adjacent vessels.This study aimed to evaluate the clinical value of cholangiography combined with spiral CT three-dimensional (3D)angiography for a preoperative assessment of hilar cholangiocarcinoma. METHODS:From March 2007 to August 2009,cholangiography was performed in 13 patients with hilar cholangiocarcinoma. Meanwhile,contrast-enhanced abdominal scanning was performed using 16-slice spiral CT,and the 3D images of the hepatic artery and portal vein were acquired.The level and range of invasion of the hepatic artery,the portal vein,and the bile duct,the preoperative Bismuth classification,and T-staging were recorded and compared with those after surgical exploration. RESULTS:The hepatic artery and portal vein were recon- structed successfully in all these patients.Percutaneous transhepatic cholangiography was performed in 9 patients,endoscopic retrograde cholangiopancreatography in 1,and magnetic resonance cholangiopancreatography in 3.The CT angiography records of invasion of the hepatic artery were consistent with the results of explorations in these patients. The data from 5 of the 13 patients were consistent with those on invasion of the portal vein.The results of the Bismuth classification and the T-staging system were consistent with those of surgical exploration in 12 of the 13 patients.Seven of 8 patients who were estimated to be suitable for operation based on images were curatively treated and 5 who were judged to be unsuitable for curative operation by cholangiography and CT angiography were confirmed intraoperatively and underwent palliative procedures. CONCLUSIONS:Cholangiography combined with multi-slice spiral 3D CT angiography can satisfactorily delineate the local invasion of hilar cholangiocarcinoma and accurately evaluate the resectability.This approach,therefore,contributes to the planning of safe operation. | Yu, Shi-An Zhang, Cheng Zhang, Jia-Min Mao, Gen-Jun Xu, Long-Tang Wu, Xiao-Kang Shu, Jin-Er Lv, Guang-Hong Zheng, Zhang-Dong | 2010 | Hepatobiliary & Pancreatic Diseases International2010，9，2： | 15 |
| 19 | A radiomic nomogram based on an apparent diffusion coefficient map for differential diagnosis of suspicious breast findings显示文摘Objective: To develop and validate a radiomic nomogram based on an apparent diffusion coefficient(ADC) map for differentiating benign and malignant lesions in suspicious breast findings classified as Breast Imaging Reporting and Data System(BI-RADS) category 4 on breast magnetic resonance imaging(MRI).Methods: Eighty-eight patients diagnosed with BI-RADS 4 findings on breast MRI in the First Affiliated Hospital of China Medical University from December 2014 to December 2015 were retrospectively analyzed in this study. Sixty-three were randomized electronically to establish forecasting models, and the other 25 were used for validation. Radiomic features based on the ADC map were generated automatically by Artificial Intelligence Kit software(A.K. software; GE Healthcare, China). Feature reduction was conducted using the Mann-Whitney test and Spearman correlation after pre-treatment. A prediction model of ADC radiomics was established by logistic linear regression and cross-validation. A nomogram was established based on ADC radiomic features,pharmacokinetics and clinical features, including the morphology and ADC value for breast BI-RADS 4 lesions on MRI.Results: A total of 396 radiomic features were extracted automatically by the A.K. software. Five features were selected after pre-processing, Mann-Whitney tests and Spearman correlation analysis. The area under the ROC curve of the prediction model comprising ADC radiomic features was 0.79 when the cutoff value was 0.45, and the accuracy, sensitivity and specificity were 80.0%, 0.813 and 0.778, respectively. A visualized differential nomogram based on the radiomic score, pharmacokinetics and clinical features was established. The decision curve showed good consistency.Conclusions: ADC radiomic features could provide an important reference for differential diagnosis between benign and malignant lesions in suspicious BI-RADS 4 lesions. The visualized nomogram based on ADC radiomic features, pharmacokinetics and clinical features may have good prospects for clinical application. | Bin Hu Ke Xu Zheng Zhang Ruimei Chai Shu Li Lina Zhang | 2018 | Chinese Journal of Cancer Research2018，30，4： | 15 |
| 20 | Characterization and Evaluation of OsLCT1 and OsN ramp5 Mutants Generated Through CRISPR/Cas9-Mediated Mutagenesis for Breeding Low Cd Rice显示文摘To explore how rice(Oryza sativa L.) can be safely produced in Cd-polluted soil, OsLCT1 and OsNramp5 mutant lines were generated by CRISPR/Cas9-mediated mutagenesis. One of OsLCT1 mutant(lct1×1) and two of OsNramp5 mutants(nramp5×7 and nramp5×9) were evaluated for grain Cd accumulation and agronomic performances. In paddy field soil containing approximately 0.9 mg/kg Cd, lct1×1 grains contained approximately 40%(0.17 mg/kg) of the Cd concentration of the wild type parental line, less than the China National Food Safety Standard(0.20 mg/kg). Both OsNramp5 mutants showed low grain Cd accumulation(< 0.06 mg/kg) in the paddy(approximately 0.9 mg/kg Cd) or in pots in soil spiked with 2 mg/kg Cd. However, only nramp5×7 showed normal growth and yield, whereas the growth of nramp5×9 was severely impaired. The study showed that lct1×1 could be used to produce rice grains safe for human consumption in lightly contaminated paddy soils and nramp5×7 used in soils contaminated by much higher levels of Cd. | LIU Songmei JIANG Jie LIU Yang MENG Jun XU Shouling TAN Yuanyuan LI Youfa SHU Qingyao HUANG Jianzhong | 2019 | Rice science2019，26，2： | 15 |
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