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| 1 | Essential roles of exosome and circRNA_101093 on ferroptosis desensitization in lung adenocarcinoma显示文摘Background:Resistance to ferroptosis,a regulated cell death caused by irondependent excessive accumulation of lipid peroxides,has recently been linked to lung adenocarcinoma(LUAD).Intracellular antioxidant systems are required for protection against ferroptosis.The purpose of the present studywas to investigate whether and how extracellular system desensitizes LUAD cells to ferroptosis.Methods:Established human lung fibroblasts MRC-5,WI38,and human LUAD H1650,PC9,H1975,H358,A549,and H1299 cell lines,tumor and matched normal adjacent tissues of LUAD,and plasma from healthy individuals and LUAD patients were used in this study.Immunohistochemistry and immunoblotting were used to analyze protein expression,and quantitative reverse transcription-PCR was used to analyze mRNA expression.Cell viability,cell death,and the lipid reactive oxygen species generationwere measured to evaluate the responses to ferroptosis.Exosomes were observed using transmission electron microscope.The localization of arachidonic acid(AA)was detected using click chemistry labeling followed by confocal microscopy.Interactions between RNAs and proteins were detected using RNA pull-down,RNA immunoprecipitation and photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation methods.Proteomic analysis was used to investigate RNA-regulated proteins,and metabolomic analysis was performed to analyze metabolites.Cellderived xenograft,patient-derived xenograft,cell-implanted intrapulmonary LUAD mouse models and plasma/tissue specimens from LUAD patients were used to validate the molecular mechanism.Results:Plasma exosome from LUAD patients specifically reduced lipid peroxidation and desensitized LUAD cells to ferroptosis.A potential explanation is that exosomal circRNA_101093(cir93)maintained an elevation in intracellular cir93 in LUAD to modulate AA,a poly-unsaturated fatty acid critical for ferroptosisassociated increased peroxidation in the plasma membrane.Mechanistically,cir93 interacted with and increased fatty acid-binding protein 3(FABP3),which transported AA and facilitated its reaction with taurine.Thus,global AA was reduced,whereas N-arachidonoyl taurine(NAT,the product of AA and taurine)was induced.Notably,the role of NAT in suppressing AA incorporation into the plasma membrane was also revealed.In pre-clinical in vivo models,reducing exosome improved ferroptosis-based treatment.Conclusion:Exosome and cir93 are essential for desensitizing LUAD cells to ferroptosis,and blocking exosome may be helpful for future LUAD treatment. | Xiao Zhang Yunhua Xu Lifang Ma Keke Yu Yongjie Niu Xin Xu Yi Shi Susu Guo Xiangfei Xue Yikun Wang Shiyu Qiu Jiangtao Cui Hong Wang Xiaoting Tian Yayou Miao Fanyu Meng Yongxia Qiao Yongchun Yu Jiayi Wang | 2022 | Cancer Communications2022，42，4： | 10 |
| 2 | Intermediate-depth earthquakes beneath the Pamir-Hindu Kush Region:Evidence for collision between two opposite subduction zones显示文摘We employed a double-difference algorithm(hypoDD)to relocate earthquakes within the region bounded by 66°E-78°E and 32°N-42°N in the period of 1964?2003 reported by the International Seismological Center(ISC).The improved hypocentral locations delineate a double-layered Wadati-Benioff zone in the eastern Hindu Kush intermediate seismic belt.Based on this feature and other evidences,we propose that the intermediate-depth earthquakes beneath the Pamir-Hindu Kush region may occur in two collided subduction zones with opposite dip directions. | Xiaoting Lou Chen Cai Chunquan Yu Jieyuan Ning | 2009 | Earthquake Science2009，22，6： | 6 |
| 3 | Auditory characteristics of noise-exposed memberscrossing age-related groups显示文摘Objective: To report audiological characteristics in a group of noise-exposed crew members on board ships.Methods and materials: Clinical and audiological measurements including pure-tone thresholds, acoustic immittance results and tinnitus questionnaires were collected from both the ship crew members(study subjects) and their land based colleagues(controls).Results: 1) Noise exposed crew members showed not only high frequency, but also low frequency hearing loss; 2) Hearing impairment increased with age, with 65.5% of crew members younger than 50 years showing normal hearing while only 14.9% of those older than 50 years had normal hearing; 3)hearing loss gradually increased with the extension of on board career time; and 4) Most study subjects reported high pitch tinnitus, significantly more than the control group although not significantly different among different age groups.Conclusion: Noise induced hearing impairment from working on board ships shows specific frequency and age characteristics. Understanding these characteristics is important for advancing relevant studies and for effective prevention of noise-induced hearing loss in ship crew members. | Liu Chenqing Ding Daxiong Zhu Yuhua Wang Hongyang Cheng Xiaoting Zhao Zhenhua Cao Juyang Zhai Suoqiang Yu Ning | 2018 | Journal of Otology2018，13，2： | 3 |
| 4 | Anti-oxidant and anti-inflammatory activities of ultrasonicassistant extracted polyphenol-rich compounds from Sargassum muticum显示文摘Polypehnol is an important,potentially bioactive component of Sargassum muticum.In this study,ultrasonic assisted extraction of polyphenol-rich substances was performed using a 38%ethanol solution at a solid:liquid ratio of 1:30 at 68℃ for 32min,determined by single-factor and response surface methodology(RSM)optimization.The content of polyphenol was 5.66mg/g in the crude extract.Further extraction showed that the polyphenol mainly distributed in ethyl acetate(SKEE)and water phases(SKEW).The anti-oxidation test by electron spin resonance(ESR)spectrum showed that the SKEE had the strongest scavenging activity on DPPH(1,1-diphenyl-2-picrylhydrazyl)and alkyl radicals.SKEE was shown noncytotoxic but could inhibit the generation of cellular ROS,showing protective effects in H2O2 and AAPHinduced Vero cells and UV-B irradiated HaCaT cells.SKEE also signifi cantly inhibited the release of NO of LPS-induced RAW 264.7 cells.Therefore,the polyphenol-rich extracts in ethanol and ethyl acetate showed excellent anti-oxidant and anti-infl ammatory activities,which is beneficial to the development of high-value bio-substances. | YU Yuan WANG Le FU Xiaoting WANG Lei FU Xiaodan YANG Min HAN Zhenlian MOU Haijin JEON You-Jin | 2019 | Journal of Oceanology and Limnology2019，37，3： | 3 |
| 5 | Rapid inactivation of multidrug-resistant bacteria and enhancement of osteoinduction via titania nanotubes grafted with polyguanidines显示文摘The rapid in situ inhibition of bacterial contamination and subsequent infection without inducing drug resistance is highly vital for the successful implantation and long-term service of titanium(Ti)-based orthopedic implants.However,the instability and potential cytotoxicity of current coatings have deterred their clinical practice.In this study,anodic oxidized titania nanotubes(TNT)were modified with antibacterial polyhexamethylene guanidine(PG)with the assistance of 3,4-dihydroxyphenylacetic acid.Interestingly,the prepared TNT-PG coating exhibited superior in vitro antibacterial activity than flat Ti-PG coating and effectively killed typical pathogens such as Escherichia coli and superbug methicillinresistant Staphylococcus aureus with above 4-log reduction(>99.99%killed)in only 5 min.TNT-PG coating also exerted excellent hemocompatibility with red blood cells and nontoxicity toward mouse pre-osteoblasts(MC3 T3-E1)in 1 week of coculture.In addition,the efficient in vivo anti-infective property of this coating was observed in a rat subcutaneous infection model.More importantly,TNT-PG coating improved the expression of alkaline phosphatase and enhanced the extracellular matrix mineralization of pre-osteoblasts,denoting its osteoinductive capacity.This versatile TNT-PG coating with excellent antibacterial activity and biocompatibility could be a promising candidate for advanced orthopedic implant applications. | Wei Feng Nian Liu Lingling Gao Qian Zhou Luofeng Yu Xiaoting Ye Jingjing Huo Xiao Huang Peng Li Wei Huang | 2021 | Journal of Materials Science & Technology2021，，10： | 2 |
| 6 | New Algorithm Rules Out Acute-on-chronic Liver Failure Development within 28 Days from Acute Decompensation of Cirrhosis显示文摘Background and Aims:Approximately 10%of patients with acute decompensated(AD)cirrhosis develop acute-on-chronic liver failure(ACLF)within 28 days.Such cases have high mortality and are difficult to predict.Therefore,we aimed to establish and validate an algorithm to identify these patients on hospitalization.Methods:Hospitalized patients with AD who developed ACLF within 28 days were considered pre-ACLF.Organ dysfunction was defined accord-ing to the chronic liver failure-sequential organ failure as-sessment(CLIF-SOFA)criteria,and proven bacterial infec-tion was taken to indicate immune system dysfunction.A retrospective multicenter cohort and prospective one were used to derive and to validate the potential algorithm,re-spectively.A miss rate of<5%was acceptable for the calcu-lating algorithm to rule out pre-ACLF.Results:In the deri-vation cohort(n=673),46 patients developed ACLF within 28 days.Serum total bilirubin,creatinine,international normalized ratio,and present proven bacterial infection at admission were associated with the development of ACLF.AD patients with≥2 organ dysfunctions had a higher risk for pre-ACLF patients[odds ratio=16.58195%confidence interval:(4.271-64.363),p<0.001].In the derivation co-hort,67.5%of patients(454/673)had≤1 organ dysfunction and two patients(0.4%)were pre-ACLF,with a miss rate of 4.3%(missed/total,2/46).In the validation cohort,65.9%of patients(914/1388)had≤1 organ dysfunction,and four(0.3%)of them were pre-ACLF,with a miss rate of 3.4%(missed/total,4/117).Conclusions:AD patients with≤1 organ dysfunction had a significantly lower risk of developing ACLF within 28 days of admission and could be safely ruled out with a pre-ACLF miss rate of<5%. | Xiaoting Tang Hai Li Guohong Deng Xin Zheng Xianbo Wang Yan Huang Yanhang Gao Zhongji Meng Zhiping Qian Feng Liu Xiaobo Lu Yu Shi Beiling Li Wenyi Gu Xiaomei Xiang Yan Xiong Yixin Hou Jun Chen Na Gao Sen Luo Liujuan Ji Jing Li Rongjiong Zheng Haotang Ren Jinjun Chen | 2023 | Journal of Clinical and Translational Hepatology2023，11，3： | 1 |
| 7 | Better prognostic determination of cT3 rectal cancer through measurement of distance to mesorectal fascia:A multicenter study显示文摘Objective:To forward the magnetic resonance imaging(MRI)based distance between the deepest tumor invasion and mesorectal fascia(DMRF),and to explore its prognosis differentiation value in cT3 stage rectal cancer with comparison of cT3 substage.Methods:This was a retrospective,multicenter cohort study including cT3 rectal cancer patients undergoing neoadjuvant chemoradiotherapy followed by radical surgery from January 2013 to September 2014.DMRF and cT3 substage were evaluated from baseline MRI.The cutoff of DMRF was determined by disease progression.Multivariate cox regression was used to test the prognostic values of baseline variables.Results:A total of 804 patients were included,of which 226(28.1%)developed progression.A DMRF cutoff of7 mm was chosen.DMRF category,the clock position of the deepest position of tumor invasion(CDTI)and extramural venous invasion(EMVI)were independent predictors for disease progression,and hazard ratios(HRs)were 0.26[95%confidence interval(95%CI),0.13-0.56],1.88(95%CI,1.33-2.65)and 1.57(95%CI,1.13-2.18),respectively.cT3 substage was not a predictor for disease progression.Conclusions:The measurement of DMRF value on baseline MRI can better distinguish cT3 rectal cancer prognosis rather than cT3 substage,and was recommended in clinical evaluation. | Xiaoyan Zhang Qiaoyuan Lu Xiangjie Guo Wuteng Cao Hongmei Zhang Tao Yu Xiaoting Li Zhen Guan Xueping Li Ruijia Sun Yingshi Sun | 2021 | Chinese Journal of Cancer Research2021，33，5： | 1 |
| 8 | Pharmacological modulation of autophagy for Alzheimer's disease therapy:Opportunities and obstacles显示文摘Alzheimer's disease(AD)is a prevalent and deleterious neurodegenerative disorder characterized by an irreversible and progressive impairment of cognitive abilities as well as the formation of amyloidβ(Aβ)plaques and neurofibrillary tangles(NFTs)in the brain.By far,the precise mechanisms of AD are not fully understood and no interventions are available to effectively slow down progression of the disease.Autophagy is a conserved degradation pathway that is crucial to maintain cellular homeostasis by targeting damaged organelles,pathogens,and disease-prone protein aggregates to lysosome for degradation.Emerging evidence suggests dysfunctional autophagy clearance pathway as a potential cellular mechanism underlying the pathogenesis of AD in affected neurons.Here we summarize the current evidence for autophagy dysfunction in the pathophysiology of AD and discuss the role of autophagy in the regulation of AD-related protein degradation and neuroinflammation in neurons and glial cells.Finally,we review the autophagy modulators reported in the treatment of AD models and discuss the obstacles and opportunities for potential clinical application of the novel autophagy activators for AD therapy. | Zhiqiang Deng Yu Dong Xiaoting Zhou Jia-Hong Lu Zhenyu Yue | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 1 |
| 9 | Pooled Plasmid Sequencing Reveals the Relationship Between Mobile Genetic Elements and Antimicrobial Resistance Genes in Clinically Isolated Klebsiella pneumoniae显示文摘Plasmids remain important microbial components mediating the horizontal gene transfer(HGT)and dissemination of antimicrobial resistance.To systematically explore the relationship between mobile genetic elements(MGEs)and antimicrobial resistance genes(ARGs),a novel strategy using single-molecule real-time(SMRT)sequencing was developed.This approach was applied to pooled conjugative plasmids from clinically isolated multidrug-resistant(MDR)Klebsiella pneumoniae from a tertiary referral hospital over a 9-month period.The conjugative plasmid pool was obtained from transconjugants that acquired antimicrobial resistance after plasmid conjugation with 53 clinical isolates.The plasmid pool was then subjected to SMRT sequencing,and 82 assembled plasmid fragments were obtained.In total,124 ARGs(responsible for resistance to b-lactam,fluoroquinolone,and aminoglycoside,among others)and 317 MGEs[including transposons(Tns),insertion sequences(ISs),and integrons]were derived from these fragments.Most of these ARGs were linked to MGEs,allowing for the establishment of a relationship network between MGEs and/or ARGs that can be used to describe the dissemination of resistance by mobile elements.Key elements involved in resistance transposition were identified,including IS26,Tn3,IS903 B,ISEcp1,and ISKpn19.As the most predominant IS in the network,a typical IS26-mediated multi-copy composite transposition event was illustrated by tracing its flanking 8-bp target site duplications(TSDs).The landscape of the pooled plasmid sequences highlights the diversity and complexity of the relationship between MGEs and ARGs,underpinning the clinical value of dominant HGT profiles. | Yan Jiang Yanfei Wang Xiaoting Hua Yue Qu Anton Y.Peleg Yunsong Yu | 2020 | Genomics, Proteomics & Bioinformatics2020，18，5： | 1 |
| 10 | Anthraquinone Covalently Modified Carbon Nanotubes for Efficient and Steady Electrocatalytic H2O2 Generation显示文摘Anthraquinone(AQ)modified carbon materials could be endowed with significantly improved oxygen re-duction reaction(ORR)activity.However,the application of these materials in the generation of hydrogen peroxide(H2O2)has been rarely investigated.For this motivation,AQ covalently modified carbon nanotube(AQ-CNT)was pur-posely synthesized for H2O2 generation.It was found that the cumulative H2O2 concentration reached up to 187.18 mg(Lh)over AQ(40)-CNT catalyst,nearly 2.0 times higher than that over CNT,and being superior to those over most carbon materials reported.The enhanced activity stemmed from the improved mass transfer fficiency of oxygen and the enhanced electrocatalytic activity.Noteworthily,the AQ(40)-CNT material exhibited satisfactory stability for H2O2 generation,which was ascribed to the strong interaction force of C-N covalent bond.The present work could provide a vital idea for designing electrode material with simultancously improved activity and stability for H2O2 gencration. | YU Fangyuan WANG Kai WANG Chuan HE Xinxia LIAO Yang ZHAO Shilin MAO Hui LI Xiaoting MA Jun | 2020 | Chemical Research in Chinese Universities2020，36，6： | 1 |
| 11 | BAX and CDKN1A polymorphisms correlated with clinical outcomes of gastric cancer patients treated with postoperative chemotherapy显示文摘 | Xiaoting Wang Youdong Lin Fenghua Lan Yinghao Yu Xuenong Ouyang Wei Liu Feilai Xie Xuzhou Wang Qiaojia Huang | 2014 | Medical Oncology2014，，： | 1 |
| 12 | Prognostic value of differential CCND1 expression in patients with resected gastric adenocarcinoma显示文摘 | Liqiang Ma Xiaoting Wang Fenghua Lan Yinghao Yu Xuenong Ouyang Wei Liu Feilai Xie Qiaojia Huang | 2015 | Medical Oncology2015，，1： | 1 |
| 13 | An Algorithm for Determination of Projectile Attitude Angles in Projectile Trajectory Prediction显示文摘A fast and accurate algorithm is established in this paper to increase the precision of ballistic trajectory prediction.The algorithm is based on the six-degree-of-freedom(6 DOF)trajectory equations,to estimate the projectile attitude angles in every measuring time.Hereby,the algorithm utilizes the Davidon-Fletcher-Powell(DFP)method to solve nonlinear equations and Doppler radar trajectory test information containing only position coordinates of the projectile to reconstruct the angular information.The″position coordinates by the test″and″angular displacements by reconstruction″at the end phase of the radar measurement are used as an initial value for the trajectory computation to extrapolate the trajectory impact point.The numerical simulations validate the proposed method and demonstrate that the estimated impact point agrees very well with the real one.Morover,other artillery trajectory can be predicted by the algorithm,and other trajectory models,such as 4 DOF and 5 DOF models,can also be incorporated into the proposed algorithm. | Zha Qicheng Rui Xiaoting Yu Hailong Zhou Qinbo | 2018 | Transactions of Nanjing University of Aeronautics and Astronautics2018，35，2： | 0 |
| 14 | Comparison of genetic impact on growth and wood traits between seedlings and clones from the same plus trees of Pinus koraiensis显示文摘To evaluate the relationships among clones and open pollinated families from the same plus trees and to select elite breeding materials,growth,and wood characteristics of 33-year-old Pinus korainsis clones and families were measured and analyzed.The results show that growth and wood characters varied significantly.The variation due to clonal effects was higher than that of family effects.The ratio of genetic to phenotypic coefficient of variation of clones in growth and wood traits was above 90%,and the repeatability of these characteristics was more than 0.8,whereas the ratio of genetic to phenotypic coefficient of variation of families was above 90%.The broad-sense heritability of all characteristics exceeded 0.4,and the narrow-sense family heritability of growth traits was less than 0.3.Growth characteristics were positively correlated with each other,but most wood properties were weakly correlated in both clones and families.Fiber length and width were positively correlated between clones and families.Using the membership function method,eleven clones and four families were selected as superior material for improved diameter growth and wood production,and two families from clonal and open-pollinated trees showed consistently better performance.Generally,selection of the best clones is an effective alternative to deployment of families as the repeatability estimates from clonal trees were higher than narrow-sense heritability estimates from open pollinated families.The results provide valuable insight for improving P.koraiensis breeding programs and subsequent genetic improvement. | Xiaoting Liu Zhimin Lu Jingui Wu Yuchun Yang Shuchun Li Lin Wu Qingshan Xu Qi Yu Li Zhao Jian Sun Wei Zheng Mulualem Tigabu Xiyang Zhao | 2023 | Journal of Forestry Research2023，34，2： | 0 |
| 15 | Generation mechanism and control of high-frequency vibration for tracked vehicles显示文摘A crawler system provides much larger ground contact,leading to excellent terrain adaptability.Due to its structural characteristics,high‐frequency vibration proportional to the vehicle speed is generated during the driving process.This is a result of the polygon and rolling effects between the track and the wheels.A field test of a tracked vehicle is performed to monitor movement signals of the chassis and a rocker arm.Their corresponding power spectral density distributions confirm the correctness of the frequency‐calculation equation.Then,a novel elastic track tensioning device with a damper is designed as a cushion between the idler and the chassis.Depending on its geometry,the equivalent damping coefficient for a dynamic model is evaluated.Subsequently,the damping is altered in response to different operating conditions by a hybrid damping fuzzy semiactive control system.The controller accounts for both chassis and track vibration.Based on the transfer matrix method for multibody systems,a dynamical model of the track system is developed.Control performances are evaluated using two numerical simulations of obstacle crossing and off‐road driving operations.Results indicate that the proposed semiactive tensioner is substantially better than the conventional one.This paper provides a novel feasible scheme for vibration reduction of tracked vehicles. | Pingxin Wang Xiaoting Rui Feifei Liu Guoping Wang Hailong Yu Bin He Junjie Gu | 2023 | International Journal of Mechanical System Dynamics2023，3，2： | 0 |
| 16 | Development of Alternative to Animal Testing on Cosmetics by Middle-out Strategy显示文摘 | Shujun Cheng Yu Chen Yihui Ke Xiaoting Qu Yao Qin Jiating Xu | 2016 | China Detergent & Cosmetics2016，1，4： | 0 |
| 17 | TBBPA rather than its main derivatives enhanced growth of endometrial cancer via p53 ubiquitination显示文摘Tetrabromobisphenol A(TBBPA)and its derivatives widely exist in various environments and biota.Although the available data indicate that TBBPA exposure is highly associated with the increased incidence of endometrial cancer(EC),the effects of TBBPA and its main derivatives on EC proliferation and the involved crucial mechanism remain unclear.The present study aimed to investigate the effects of TBBPA and its derivatives under environmental concentrations on the proliferation of EC,and the crucial mechanism on the progression of EC caused by bromine fame retardants exposure.In this research,TBBPA and two of the most common TBBPA derivatives including TBBPA bis(2-hydroxyethyl ether)(TBBPABHEE)and TBBPA bis(dibromopropyl ether)(TBBPA-BDBPE)were screened for their capacities in induced EC proliferation and explored the related mechanism by in vitro cell culture model and in vivo mice model.Under environmental concentrations,TBBPA promoted the proliferation of EC,the main derivatives of TBBPA(TBBPA-BHEE and TBBPA-BDBPE)did not present the similar facilitation effects.The ubiquitination degradation of p53 was crucial in TBBPA induced EC proliferation,which resulted in the increase of downstream cell cycle and decrease of apoptosis.The further molecular docking result suggested the high affinity between TBBPA and ubiquitinated proteasome.This finding revealed the effects of TBBPA and its derivatives on EC proliferation,thus providing novel insights into the underlying mechanisms of TBBPA-caused EC. | Hongyan Yu Jingxu Zhang Jing Liu Ruonan Pan Yu Wang Xiaoting Jin Rifat Zubair Ahmed Yuxin Zheng | 2024 | Journal of Environmental Sciences2024，，3： | 0 |
| 18 | Less pressure contributes to gravity-driven membrane ultrafiltration with greater performance:Enhanced driving efficiency and reduced disinfection by-products formation potential显示文摘Gravity-driven membrane(GDM)systems have been well developed previously;however,impacts of driving(i.e.,transmembrane)pressure on their performance received little attention,which may infuence GDM performance.In this study,we evaluated 4 GDM systems via altering the transmembrane pressure from 50 mbar to 150 mbar with 2 groups,treating surface water in Beijing,China.Results showed that less driving pressure was more favorable.Specifically,compared to groups(150 mbar),groups under a pressure of 50 mbar were found to have greater normalized permeability and lower total resistance.During the whole operation period,the quality of effuents was gradually improved.For example,the removal efficiency of UV254was significantly improved;particularly,under low driving pressure,the removal efficiency of UV254in PES GDM system increased by 11.91%,as compared to the corresponding system under high driving pressure.This observation was consistent with the reduction on disinfection by-products(DBPs)formation potential;groups under 50 mbar achieved better DBPs potential control,indicating the advantages of lower driving pressure.Biofilms were analyzed and responsible for these differences,and distinct distributions of bacteria communities of two GDM systems under 50 and 150 mbar may be responsible for various humic-like substances removal efficiency.Overall,GDM systems under less pressure should be considered and expected to provide suggestions on the design of GDM systems in real applications. | Xiaoting Wu Yufei Li Zhaoyang Su Long Tian Muhammad Saboor Siddique Wenzheng Yu | 2024 | Journal of Environmental Sciences2024，，3： | 0 |
| 19 | B-lymphoid tyrosine kinase-mediated FAM83A phosphorylation elevates pancreatic tumorigenesis through interacting with β-catenin显示文摘Abnormal activation of Wnt/β-catenin-mediated transcription is closely associated with the malignancy of pancreatic cancer.Family with sequence similarity 83 member A(FAM83A)was shown recently to have oncogenic effects in a variety of cancer types,but the biological roles and molecular mechanisms of FAM83A in pancreatic cancer need further investigation.Here,we newly discovered that FAM83A binds directly toβ-catenin and inhibits the assembly of the cytoplasmic destruction complex thus inhibiting the subsequent phosphorylation and degradation.FAM83A is mainly phosphorylated by the SRC non-receptor kinase family member BLK(B-lymphoid tyrosine kinase)at tyrosine 138 residue within the DUF1669 domain that mediates the FAM83A-β-catenin interaction.Moreover,FAM83A tyrosine 138 phosphorylation enhances oncogenic Wnt/β-catenin-mediated transcription through promotingβ-catenin-TCF4 interaction and showed an elevated nucleus translocation,which inhibits the recruitment of histone deacetylases by TCF4.We also showed that FAM83A is a direct downstream target of Wnt/β-catenin signaling and correlates with the levels of Wnt target genes in human clinical pancreatic cancer tissues.Notably,the inhibitory peptides that target the FAM83A-β-catenin interaction significantly suppressed pancreatic cancer growth and metastasis in vitro and in vivo.Our results revealed that blocking the FAM83A cascade signaling defines a therapeutic target in human pancreatic cancer. | Cefan Zhou Xiaoting Zhu Nanxi Liu Xueying Dong Xuewen Zhang Huili Huang Yu Tang Shicheng Liu Mengyu Hu Ming Wang Xiaoling Deng Shi Li Rui Zhang Yuan Huang Hao Lyu Shuai Xiao Sang Luo Declan William Ali Marek Michalak Xing-Zhen Chen Zhentian Wang Jingfeng Tang | 2023 | Signal Transduction and Targeted Therapy2023，8，3： | 0 |
| 20 | simple and effective aerosol pathogen disinfection test for a flowing air disinfector显示文摘Aerosol transmission is an important disease transmission route and has been especially pertinent to hospital and biosafety laboratories during the SARS-CoV-2 pandemic.The thermal resistance of airborne SARS-CoV-2 is lower than that of Bacillus subtilis spores,which are often used to test the effectiveness of SARS-CoV-2 and other pathogen disinfection methods.Herein,we propose a new method to test the disinfection ability of a flowing air disinfector(a digital electromagnetic induction air heater)using B.subtilis spores.The study provides an alternative air disinfection test method.The new test system combined an aerosol generator and a respiratory filter designed in-house and could effectively recover spores on the filter membrane at the air outlet after passing through the flowing air disinfector.The total number of bacterial spores used in the test was within the range of 5×10^(5)–5×10^(6)colony-forming units(CFUs)specified in the technical standard for disinfection.The calculation was based on the calculation method in Air Disinfection Effect Appraisal Test in Technical Standard for Disinfection(2002 Edition).At an air speed of 3.5 m/s,we used a digital electromagnetic induction air heater to disinfect flowing air containing 4.100×10^(6)CFUs of B.subtilis spores and determined that the minimum disinfection temperature was 350℃for a killing rate of 99.99%.At 400℃,additional experiments using higher spore concentrations(4.700×10^(6)±1.871×10^(5)CFU)and a higher airspeed(4 m/s)showed that the killing rate remained>99.99%.B.subtilis spores,as a biological indicator for testing the efficiency of dry-heat sterilization,were killed by the high temperatures used in this system.The proposed method used to test the flowing air disinfector is simple,stable,and effective.This study provides a reference for the development of test systems that can assess the disinfection ability of flowing air disinfectors. | Xuling Liu Zhiran Qin Linqing Wang Xiaoting Xie Yifang Fu Jianhai Yu Zuxin Liang Xiaoen He Jingshu Li Hong Dai Jinxiu Yao Qinghua Wu Weiwei Xiao Li Zhu Chengsong Wan Bao Zhang Wei Zhao | 2023 | Journal of Biosafety and Biosecurity2023，5，1： | 0 |
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