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| 1 | Early Paleozoic ridge subduction in the Chinese Altai: Insight from the abrupt change in zircon Hf isotopic compositions显示文摘Zircons were separated from granitoids, gneisses, and sedimentary rocks of the Chinese Altai. Those with igneous characteristics yielded U-Pb ages of 280-2800 Ma, recording a long history of magmatic activity in the region. Zircon Hf isotopic compositions show an abrupt change at ~420 Ma, indicating that prior to that time the magmas came from both ancient and juvenile sources, whereas younger magmas were derived mainly from juvenile material. This may imply that the lithosphere was signifi- cantly modified in composition by a rapid addition of melt from the mantle. We suggest that this dramatic change was due to the onset of ridge subduction, which can account not only for the formation of voluminous granitoids, mafic rocks with complex compositions, and the association of adakite + high-Mg andesite + boninite + Nb-enriched basalt, but also for the coeval high-T, low-P metamorphism. | SUN Min LONG XiaoPing CAI KeDa JIANG YingDe WANG BuYun YUAN Chao ZHAO GuoChun XIAO WenJiao WU FuYuan | 2009 | Science China Earth Sciences2009，52，9： | 33 |
| 2 | Recent advances in gel polymer electrolyte for high-performance lithium batteries显示文摘Lithium batteries (LBs) have become increasingly important energy storage systems in our daily life. However, their practical applications are still severely plagued by the safety issues from liquid electrolyte, especially when the batteries are exposed to mechanical, thermal, or electrical abuse conditions. Gel polymer electrolytes (GPEs) are being considered as an effective solution to replace currently available organic liquid electrolyte for building safer LBs. This review provides recent advancements in GPEs applied for high-performance LBs. On the one hand, from the environmental and economic point of view, the skeletons of GPEs changed from traditional polymer to renewable and degradable polymer. On the other hand, in addition to being as a component with good electrochemical and physical characterizations, the GPEs also need to provide some functions for addressing the concerns of lithium (Li) dendrites, unstable cathode electrolyte interface, dissolution and migration of transition metal ions,'shuttle effect' of polysulfides, and so on. Finally, to synchronously meet the challenges from the advanced cathode and Li metal anode, the bio-based GPEs with multi-functionality are proposed to develop high-energy/powerdensity batteries in the future. | Ming Zhu Jiaxin Wu Yue Wang Mingming Song Lei Long Sajid Hussain Siyal Xiaoping Yang Gang Sui | 2019 | Journal of Energy Chemistry2019，28，10： | 17 |
| 3 | The discovery of the oldest rocks in the Kuluketage area and its geological implications显示文摘The basement rocks in the Kuluketage area are composed predominately of tonalite-trondhjemite-granodiorite rocks, and occured mainly in Xinger and Korla. U-Pb dating of TTG gneiss near Korla yielded a late Neoarchean weighted mean 207Pb/206Pb age of 2.65 Ga, which is the oldest published age for the TTG rocks in the Kuluketage area and thus suggests that Archean terrane in the area was formed in the late Neoarchean. The Korla gneiss is much younger than the TTG rocks in the northern Altyn Tagh, eastern Tarim Craton, indicating that the oldest terrane of the Tarim Craton was exposed probably in the northern Altyn Tagh. Until late Neoarchean, the Tarim continent extends to the Kuluketage area and finally had generated a relatively large uniform Archean basement within the craton. Zircon Hf isotopic analyses of the TTG gneiss give low εHf(t) values (-5 to 1) with Paleoarchean to Mesoarchean two-stage model ages (TDM2) between 3.0 and 3.3 Ga, suggesting that the basement rocks in the northern Tarim Craton were derived dominately from partial melting of Paleoarchean to Mesoarchean juvenile crustal material. The Hf model ages, therefore, indicate that no continent crust older than 3.3 Ga existed in the Kuluketage area. | LONG XiaoPing YUAN Chao SUN Min XIAO WenJiao ZHAO GuoChun ZHOU KeFa WANG YuJing HU AiQin | 2011 | Science China Earth Sciences2011，54，3： | 16 |
| 4 | Prevalence of vitamin D deficiency in girls with idiopathic central precocious puberty显示文摘在维生素 D 缺乏和自发的中央早熟的发身(ICPP ) 之间的关系最近被记录了。在这研究, 280 个女孩与 ICPP 诊断了,类似的年龄的 188 个正常发身控制女孩被注册并且回顾地学习了。有的 ICPP 组显著地降低浆液 25-hydroxyvitamin D (25 [哦] D ) 层次比控制组。而且,一种非线性的关系在浆液 25 之间被发现[哦] D 和 ICPP,和为浆液 25 的一个截止的点[哦] D 有或没有调整不同惊讶因素为 ICPP 在 31.8 ng/ml 被发现。有浆液 25 的女孩[哦] D31.8 ng/ml 有更低的机会比率(unadjusted:或 0.36, 95% CI 0.15 到 0.83, P < 0.05;调整的高度和重量:或 0.44, 95% CI 0.18 到 1.08, P = 0.072;BMI 调整了:或 0.36, 95% CI 0.16 到 0.84, P < 0.05 ) 。有 25 的 ICPP 题目[哦] D 缺乏从二另外的亚群比这些题目有一个更高的身体团索引(BMI ) 。关联分析证明那维生素 D 水平在 ICPP 组与 BMI 和一些新陈代谢的参数被相关。我们的学习建议那维生素 D 地位可以与 ICPP 风险被联系并且可以在 ICPP 上有阀值效果。 | Yue Zhao Wenjun Long Caiqi Du Huanhuan Yang Shimin Wu Qin Ning Xiaoping Luo | 2018 | Frontiers of Medicine2018，12，2： | 16 |
| 5 | Carbon pools and fluxes in the China Seas and adjacent oceans显示文摘The China Seas include the South China Sea, East China Sea, Yellow Sea, and Bohai Sea. Located off the Northwestern Pacific margin, covering 4700000 km^2 from tropical to northern temperate zones, and including a variety of continental margins/basins and depths, the China Seas provide typical cases for carbon budget studies. The South China Sea being a deep basin and part of the Western Pacific Warm Pool is characterized by oceanic features; the East China Sea with a wide continental shelf, enormous terrestrial discharges and open margins to the West Pacific, is featured by strong cross-shelf materials transport; the Yellow Sea is featured by the confluence of cold and warm waters; and the Bohai Sea is a shallow semiclosed gulf with strong impacts of human activities. Three large rivers, the Yangtze River, Yellow River, and Pearl River, flow into the East China Sea, the Bohai Sea, and the South China Sea, respectively. The Kuroshio Current at the outer margin of the Chinese continental shelf is one of the two major western boundary currents of the world oceans and its strength and position directly affect the regional climate of China. These characteristics make the China Seas a typical case of marginal seas to study carbon storage and fluxes. This paper systematically analyzes the literature data on the carbon pools and fluxes of the Bohai Sea,Yellow Sea, East China Sea, and South China Sea, including different interfaces(land-sea, sea-air, sediment-water, and marginal sea-open ocean) and different ecosystems(mangroves, wetland, seagrass beds, macroalgae mariculture, coral reefs, euphotic zones, and water column). Among the four seas, the Bohai Sea and South China Sea are acting as CO_2 sources, releasing about0.22 and 13.86–33.60 Tg C yr^(-1) into the atmosphere, respectively, whereas the Yellow Sea and East China Sea are acting as carbon sinks, absorbing about 1.15 and 6.92–23.30 Tg C yr^(-1) of atmospheric CO_2, respectively. Overall, if only the CO_2 exchange at the sea-air interface is considered, the Chinese marginal seas appear to be a source of atmospheric CO_2, with a net release of 6.01–9.33 Tg C yr^(-1), mainly from the inputs of rivers and adjacent oceans. The riverine dissolved inorganic carbon (DIC) input into the Bohai Sea and Yellow Sea, East China Sea, and South China Sea are 5.04, 14.60, and 40.14 Tg C yr^(-1),respectively. The DIC input from adjacent oceans is as high as 144.81 Tg C yr^(-1), significantly exceeding the carbon released from the seas to the atmosphere. In terms of output, the depositional fluxes of organic carbon in the Bohai Sea, Yellow Sea, East China Sea, and South China Sea are 2.00, 3.60, 7.40, and 5.92 Tg C yr^(-1), respectively. The fluxes of organic carbon from the East China Sea and South China Sea to the adjacent oceans are 15.25–36.70 and 43.93 Tg C yr^(-1), respectively. The annual carbon storage of mangroves, wetlands, and seagrass in Chinese coastal waters is 0.36–1.75 Tg C yr^(-1), with a dissolved organic carbon(DOC) output from seagrass beds of up to 0.59 Tg C yr^(-1). Removable organic carbon flux by Chinese macroalgae mariculture account for 0.68 Tg C yr^(-1) and the associated POC depositional and DOC releasing fluxes are 0.14 and 0.82 Tg C yr^(-1), respectively. Thus, in total, the annual output of organic carbon, which is mainly DOC, in the China Seas is 81.72–104.56 Tg C yr^(-1). The DOC efflux from the East China Sea to the adjacent oceans is 15.00–35.00 Tg C yr^(-1). The DOC efflux from the South China Sea is 31.39 Tg C yr^(-1). Although the marginal China Seas seem to be a source of atmospheric CO_2 based on the CO_2 flux at the sea-air interface, the combined effects of the riverine input in the area, oceanic input, depositional export,and microbial carbon pump(DOC conversion and output) indicate that the China Seas represent an important carbon storage area. | Nianzhi JIAO Yantao LIANG Yongyu ZHANG Jihua LIU Yao ZHANG Rui ZHANG Meixun ZHAO Minhan DAI Weidong ZHAI Kunshan GAO Jinming SONG Dongliang YUAN Chao LI Guanghui LIN Xiaoping HUANG Hongqiang YAN Limin HU Zenghu ZHANG Long WANG Chunjie CAO Yawei LUO Tingwei LUO Nannan WANG Hongyue DANG Dongxiao WANG Si ZHANG | 2018 | Science China Earth Sciences2018，61，11： | 12 |
| 6 | Petrogenesis and Geodynamic Implications of the Carboniferous Granitoids in the Dananhu Belt,Eastern Tianshan Orogenic Belt显示文摘This paper presents new LA-ICP-MS zircon U-Pb geochronology,whole-rock major and trace element geochemistry,and Sr-Nd isotopes systematically on porphyritic granitic and K-feldspar granitic intrusions from the Dananhu belt,eastern Tianshan orogenic belt(ETOB).Zircon U-Pb dating indicates that the porphyritic granitic and K-feldspar granitic plutons were formed at 357±3 and 311±3 Ma,respectively.The porphyritic granites show geochemical and isotopic characteristics(high Si O2,low Mg O and Mg^#,depleted Sr-Nd isotopic values(about 0.7034 and 6.13,respectively),with Nb/Ta(13.3–14.7)and Zr/Hf(31.0–33.9)ratios)similar to those of the crustal-derived magmas.The above characteristics suggest they were probably originated from juvenile lower crustal materials.The K-feldspar granites also have high Si O2,low Mg O and Mg^#,depleted Sr-Nd isotopic values(0.7033–0.7046 and 4.41–5.67,respectively).But some trace elements contents vary widely,with variable Nb/Ta(12.7–22.7),Zr/Hf(21.3–36.1)and Nb/La(0.38–1.07)ratios,indicating that the K-feldspar granites were formed by partial melting of juvenile lower crustal materials with old crustal materials.Combined with previous data on Carboniferous granitoids in the Dananhu belt,we infer that all the Carboniferous granitic plutons in the Dananhu belt were most likely emplaced in an island arc environment(Dananhu arc).Subsequently,a tectonic transition from oceanic subduction to post-collisional extension probably occurred in the ETOB. | Long Du Chao Yuan Xu-Ping Li Yunying Zhang Zongying Huang Xiaoping Long | 2019 | Journal of Earth Science2019，30，6： | 6 |
| 7 | Expression of human FUS/TLS in yeast leads to protein aggregation and cytotoxicity,recapitulating key features of FUS proteinopathy显示文摘Mutations in the fused in sarcoma/translocated in liposarcoma(FUS/TLS)gene have been associated with amyotrophic lateral sclerosis(ALS).FUS-positive neuropathology is reported in a range of neurodegenerative diseases,including ALS and fronto-temporal lobar degeneration with ubiquitin-positive pathology(FTLDU).To examine protein aggregation and cytotoxicity,we expressed human FUS protein in yeast.Expression of either wild type or ALS-associated R524S or P525L mutant FUS in yeast cells led to formation of aggregates and cytotoxicity,with the two ALS mutants showing increased cytotoxicity.Therefore,yeast cells expressing human FUS protein recapitulate key features of FUSpositive neurodegenerative diseases.Interestingly,a significant fraction of FUS expressing yeast cells stained by propidium iodide were without detectable protein aggregates,suggesting that membrane impairment and cellular damage caused by FUS expression may occur before protein aggregates become microscopically detectable and that aggregate formation might protect cells from FUS-mediated cytotoxicity.The N-terminus of FUS,containing the QGSY and G rich regions,is sufficient for the formation of aggregates but not cytotoxicity.The C-terminal domain,which contains a cluster of mutations,did not show aggregation or cytotoxicity.Similar to TDP-43 when expressed in yeast,FUS protein has the intrinsic property of forming aggregates in the absence of other human proteins.On the other hand,the aggregates formed by FUS are thioflavin T-positive and resistant to 0.5%sarkosyl,unlike TDP-43 when expressed in yeast cells.Furthermore,TDP-43 and FUS display distinct domain requirements in aggregate formation and cytotoxicity. | Kazuo Fushimi Charles Long Neha Jayaram Xiaoping Chen Liming Li Jane Y.Wu | 2011 | Protein & Cell2011，2，2： | 4 |
| 8 | Geochronology and geochemistry of Late Carboniferous dykes in the Aqishan-Yamansu belt,eastern Tianshan:Evidence for a post-collisional slab breakoff显示文摘With aim of providing constraints on the Late Paleozoic tectonic evolution of the southern Central Asian Orogenic Belt(CAOB),an integrated study was conducted on the geochronological and geochemical data for dioritic,granitic and diabase dykes from the Aqishan-Yamansu belt in the eastern Tianshan,NW China.Zircon U-Pb dating indicates that the dioritic and granitic dykes were both emplaced in the Late Carboniferous(~311 Ma and^315 Ma).The dioritic dykes show adakitic characteristics and have high Na2 O and positiveεHf(t)values(+12 to+17),which suggest an origin from partial melts of a subducted oceanic slab.The granitic dykes have high SiO2 and K2 O contents and are characterized by en riched light rare earth elements(LREE)and slightly flat heavy rare earth elements(HREE),with negative Eu and Nb-Ta-Ti anomalies.These dykes are alkali-calcic and show geochemical features of highly fractionated Itype granites.Their positiveεHf(t)values(+16 to+17)suggest that they were derived from a juvenile accreted oceanic crustal sou rce.The coeval diabase dykes have low SiO2 and K2 O contents but high TiO2,MgO and Mg#(54-59).They are enriched in LREE and show characteristics of enriched mid-ocean ridge basalts(E-MORB).The relatively high Ba/Th,slightly low Th/Ta ratios,and negative Nb-Ta anomalies imply a mantle source metasomatised by slab-derived fluids.Thus,these basic dykes were generated likely by partial melting of the upwelling asthenosphere mantle with a slight influence of slab-derived fluids.Therefore,we suggest that the formation of these Late Carboniferous dykes were triggered by a post-collisional slab breakoff and the Aqishan-Yamansu belt was a continental arc formed by southdipping subduction of the Kangguer oceanic plate. | Xiaoping Long Bin Wu Min Sun Chao Yuan Wenjiao Xiao Rui Zuo | 2020 | Geoscience Frontiers2020，11，1： | 3 |
| 9 | The adaptive strategies of yaks to live in the Asian highlands显示文摘The yak(Bos grunniens),an indigenous herbivore raised at altitudes between 3,000 and 5,000 m above sea level,is closely linked to more than 40 ethnic communities and plays a vital role in the ecological stability,livelihood security,socio-economic development,and ethnic cultural traditions in the Asian highlands.They provide the highlanders with meat,milk,fibres,leather and dung(fuel).They are also used as pack animals to transport goods,for travel and ploughing,and are important in many religious and traditional ceremonies.The Asian highlands are known for an extremely,harsh environment,namely low air temperature and oxygen content and high ultraviolet light and winds.Pasture availability fluctuates greatly,with sparse pasture of poor quality over the long seven-month cold winter.After longterm natural and artificial selections,yaks have adapted excellently to the harsh conditions:1)by genomics,with positively selected genes involved in hypoxia response and energy metabolism;2)anatomically,including a short tongue with a weak sense of taste,and large lung and heart;3)physiologically,by insensitivity to hypoxic pulmonary vasoconstriction,maintaining foetal haemoglobin throughout life,and low heart rate and heat production in the cold season;4)behaviourlly,by efficient grazing and selecting forbs with high nutritional contents;5)by low nitrogen and energy requirements for maintenance and low methane emission and nitrogen excretion,namely,‘Low-Carbon’and‘Nitrogen-Saving’traits;6)by harboring unique rumen microbiota with a distinct maturation pattern,that has coevolved with host metabolism.This review aims to provide an overview of the comprehensive adaptive strategies of the yak to the severe conditions of the highlands.A better understanding of these strategies that yaks employ to adapt to the harsh environment could be used in improving their production,breeding and management,and gaining benefits in ecosystem service and a more resilient livelihood to climate change in the Asian highlands. | Xiaoping Jing Luming Ding Jianwei Zhou Xiaodan Huang Allan Degen Ruijun Long | 2022 | Animal Nutrition2022，，2： | 2 |
| 10 | Ammonia-induced oxidative stress triggered proinflammatory response and apoptosis in pig lungs显示文摘Ammonia,a common toxic gas,is not only one of the main causes of haze,but also can enter respiratory tract and directly affect the health of humans and animals.Pig was used as an animal model for exploring the molecular mechanism and dose effect of ammonia toxicity to lung.In this study,the apoptosis of type II alveolar epithelial cells was observed in high ammonia exposure group using transmission electron microscopy.Gene and protein expression analysis using transcriptome sequencing and western blot showed that low ammonia exposure induced T-cell-involved proinflammatory response,but high ammonia exposure repressed the expression of DNA repair-related genes and affected ion transport.Moreover,high ammonia exposure significantly increased 8-hydroxy-2-deoxyguanosine (8-OHdG) level,meaning DNA oxidative damage occurred.In addition,both low and high ammonia exposure caused oxidative stress in pig lungs.Integrated analysis of transcriptome and metabolome revealed that the up-regulation of LDHB and ND2 took part in high ammonia exposure-affected pyruvate metabolism and oxidative phosphorylation progress,respectively.Inclusion,oxidative stress mediated ammonia-induced proinflammatory response and apoptosis of porcine lungs.These findings may provide new insights for understanding the ammonia toxicity to workers in livestock farms and chemical fertilizer plants. | Daojie Li Long Shen Di Zhang Xiaotong Wang Qiankun Wang Wenhao Qin Yun Gao Xiaoping Li | 2023 | Journal of Environmental Sciences2023，，4： | 2 |
| 11 | Influence of RNA interference on the mitochondrial subcellular localization of alpha-synuclein and on the formation of Lewy body-like inclusions in the cytoplasm of human embryonic kidney 293 cells induced by the overexpression of alphasynuclein显示文摘The specific and effective α-synuclein RNA interference (RNAi) plasmids, and the α-synuclein-pEGFP recombinant plasmids were co-transfected into human embryonic kidney 293 (HEK293) cells using the lipofectamine method. Using an inverted fluorescence microscope, α-synuclein proteins were observed to aggregate in the cytoplasm and nucleus. Wild-type α-synuclein proteins co-localized with mitochondria. Hematoxylin-eosin staining revealed round eosinophilic bodies (Lewy body-like inclusions) in the cytoplasm of some cells transfected with α-synuclein-pEGFP plasmid. However, the formation of Lewy body-like inclusions was not observed following transfection with the RNAi pSYN-1 plasmid. RNAi blocked Lewy body-like inclusions in the cytoplasm of HEK293 cells induced by wild-type α-synuclein overexpression, but RNAi did not affect the subcellular localization of wild-type α-synuclein in mitochondria. | Tao Chen Xiaoping Liao Guoqiang Wen Yidong Deng Min Guo Zhigang Long Feng Ouyang | 2012 | Neural Regeneration Research2012，7，2： | 2 |
| 12 | Conserved and differentiated functions of CIK receptor kinases in modulating stem cell signaling in Arabidopsis显示文摘The shoot apical meristem(SAM)and root apical meristem(RAM)act as pools of stem cells that give rise to aboveground and underground tissues and organs in higher plants,respectively.The CLAVATA3(CLV3)-WUSCHEL(WUS)negative-feedback loop acts as a core pathway controlling SAM homeostasis,while CLV3/EMBRYO SURROUNDING REGION(ESR)40(CLE40)and WUSCHEL-RELATED HOMEOBOX5(WOX5),homologs of CLV3 and WUS,direct columella stem cell fate.Moreover,CLV3 INSENSITIVE KINASES(CIKs)have been shown to be essential for maintaining SAM homeostasis,whereas whether they regulate the distal root meristem remains to be elucidated.Here,we report that CIKs are indispensable for transducing the CLE40 signal to maintain homeostasis of the distal root meristem.We found that the cik mutant roots displayed disrupted quiescent center and delayed columella stem cell(CSC)differentiation.Biochemical assays demonstrated that CIKs interact with ARABIDOPSIS CRINKLY4(ACR4)in a ligand-independent manner and can be phosphorylated by ACR4 in vitro.In addition,the phosphorylation of CIKs can be rapidly induced by CLE40,which partially depends on ACR4.Although CIKs act as conserved and redundant regulators in the SAM and RAM,our results demonstrated that they exhibit differentiated functions in these meristems. | Yafen Zhu Chong Hu Yanwei Cui Li Zeng Sunjingnan Li Mingsong Zhu Fanhui Meng Shuting Huang Li Long Jing Yi Jia Li Xiaoping Gou | 2021 | Molecular Plant2021，14，7： | 2 |
| 13 | Geochemistry, zircon U–Pb ages and Lu–Hf isotopes of early Paleozoic plutons in the northwestern Chinese Tianshan: Petrogenesis and geological implications显示文摘 | Zongying Huang Xiaoping Long Alfred Kr?ner Chao Yuan Qiang Wang Min Sun Guochun Zhao Yujing Wang | 2013 | LITHOS2013，，： | 1 |
| 14 | Sup- pression for dispersion induced phase noise of an opti- cally generated millimeter wave employing optical spec- trum processing 显示文摘 | Li Long Zhang Guoqiang Zheng Xiaoping | 2012 | Opt Lett2012，37，19： | 1 |
| 15 | Gaussian Process Modeling of Process Optimization and Parameter Correlation for Injection Molding显示文摘 | Xiaoping Liao Wei Xia Fengying Long | 2010 | 材料科学与工程(中英文版)2010，4，10： | 1 |
| 16 | HIF-1-Mediated Suppression of Acyl-CoA Dehydrogenases and Fatty Acid Oxidation Is Critical for Cancer Progression显示文摘 | De Huang Tingting Li Xinghua Li Long Zhang Linchong Sun Xiaoping He Xiuying Zhong Dongya Jia Libing Song Gregg L. Semenza Ping Gao Huafeng Zhang | 2014 | Cell Reports2014，，6： | 1 |
| 17 | The growth of H-harmonic functions on the Heisenberg group显示文摘We discuss the relationship between the frequency and the growth of H-harmonic functions on the Heisenberg group.Precisely,we prove that an H-harmonic function must be a polynomial if its frequency is globally bounded.Moreover,we show that a class of H-harmonic functions are homogeneous polynomials provided that the frequency of such a function is equal to some constant. | LIU HaiRong TIAN Long YANG XiaoPing | 2014 | Science China Mathematics2014，57，4： | 1 |
| 18 | RNA interference blocking the apoptosis in HEK293 cells induced by overexpression of alpha-synuclein显示文摘BACKGROUND:Overexpression ofα-synuclein can induce cell apoptosis.RNA interference(RNAi) may block specific gene function and cause gene silencing. OBJECTIVE:To construct a specific and effective RNAi plasmid for theα-synuclein gene and investigate if RNAi can block apoptosis in HEK293 cells,induced by overexpression of wild-typeα-synuclein. DESIGN,TIME AND SETTING:A contrast experiment based on genetically engineered cytobiology was performed at the State Key Lab of Medical Genetics of China,Xiangya Medical College of Central South University,between October 2004 and October 2008. MATERIALS:HEK293 cells and pBSHH1 plasmid were provided by the State Key Lab of Medical Genetics of China;OligDNA sequence by Sagon Bioengineering Company,Shanghai; Lipofectamine 2000 by Invitrogen,USA;α-synuclein monoclonal antibody,Hoechst 33258,and MTT; by Sigma,USA;Horseradish peroxidase-coupled goat anti-rat IgG by KPL,USA;FACSan flow cytometry by BD,USA. METHODS:Four target sites were used to construct hairpin RNA pBSHH1 vectors-pSYNi-1, pSYNi-2,pSYNi-3 and pSYNi-4-which were cloned in the pBSHH1 plasmid.HEK293 cells were transfected using Lipofectamine 2000.In addition,a non-transfect group and a negative plasmid transfect group were established.The cultured HEK293 cells were processed as follows: transfection of blank plasmid(blank control group),transfection ofα-synuclein-pEGFP and RNAi negative vector(negative control group),and transfection ofα-synuclein-pEGFP and pSYNi-1 (transfection group).Cells in all groups were transfected with Lipofectamine 2000 for 48 hours. MAIN OUTCOME MEASURES:Expression ofα-synuclein mRNA and protein were detected by RT-PCR and Western blot.Cell morphology was observed under an inverted fluorescence microscope;cell viability was measured using MTT method;and cell apoptosis was determined with Annexin V-PE flow cytometry. RESULTS:α-synuclein mRNA and protein expressions were significantly decreased in the pSYNi-1 group when compared with the non-transfect and negative plasmid transfect groups(P<0.05).The expressions were partially decreased in the pSYNi-2 group,but there was no significant difference in the pSYNi-3 and pSYNi-4 groups.Hoechst staining indicated that cell nuclei were enlarged in the negative control group,coloring was not uniform,and chromatin was accumulated and appeared spot-like.The nucleus coloring was uniform in the transfection group compared to negative control group.Cell viability in the negative control group was significantly lower than blank control group with cell apoptosis being significantly increased(P<0.05).In comparison with negative control group, cell viability was significantly increased in the transfection group and cell apoptosis was significantly decreased(P<0.05). CONCLUSION:pSYNi-1 can inhibitα-synuclein gene expression and block apoptosis of HEK293 cells induced by overexpression of wild-typeα-synuclein. | Tao Chen Beisha Tang Xiaoping Liao Guoqiang Wen Xinxiang Yan Jifeng Guo Yuhu Zhang Feng Ouyang Zhigang Long Li Cao Jing Li | 2009 | Neural Regeneration Research2009，4，7： | 1 |
| 19 | Geochronological and geochemical study of mafic dykes from the northwest Chinese Altai: Implications for petrogenesis and tectonic evolution显示文摘 | Keda Cai Min Sun Chao Yuan Guochun Zhao Wenjiao Xiao Xiaoping Long Fuyuan Wu | 2010 | Gondwana Research2010，，4： | 1 |
| 20 | Accretionary orogenesis of the Chinese Altai: Insights from Paleozoic granitoids显示文摘 | Chao Yuan Min Sun Wenjiao Xiao Xianhua Li Hanlin Chen Shoufa Lin Xiaoping Xia Xiaoping Long | 2007 | Chemical Geology2007，，1： | 1 |
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